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The map label for this gene is eno

Identifier: 71891945

GI number: 71891945

Start: 206853

End: 208151

Strand: Direct

Name: eno

Synonym: BPEN_162

Alternate gene names: 71891945

Gene position: 206853-208151 (Clockwise)

Preceding gene: 71891944

Following gene: 71891946

Centisome position: 26.13

GC content: 33.03

Gene sequence:

>1299_bases
ATGCCTAAGATTTTAAATATTGTTGGACGTGAGGTTATTGATTCTCGTGGTAATCCAACAGTAGAGACCGAAGTATATAT
CAAAGGTGGTTCTAGATTAGCATCTGTTCCGTCTGGAGCATCTGTTGGTTCTCAAGAAGCTTTAGAGTTAAGGGATAATA
ATAACAATCGGTTTTTTGGAAAAGGGGTCACAAAAGCGGTCAGTTTTGTAAATGGACCCATTCATAAAGCATTAATAGGA
ATGGATGTAACGCAACAACGTGTTATTGATACTATTATGATTGATTTGGATGGAACCAGTAATAAATCAAAATTTGGTGC
TAATGCTATCTTGAGCGTTTCTTTAGCAGTTGCAAAAGCAGCCTCGGCATTTAAAAATGTTCCATTATATCAGCATATTT
CTGATTTGTATGACGCGCCAAAAAATACACTTGTTATGCCACTCCCTATGATGAATATTATTAATGGCGGTAAGCATGCT
GATAATAATTTAGATATTCAGGAATTTATGATTATCCCGATTGGAGCAAAAACCATCAAAGAAGCAATCCAGATGGGATC
TGAAATATCATATAATTTAAGAGTAATATTAAGTAATAAAGGAATATCCACGCAATTAGGTGACGAAGGTGGATATGCTC
CTAATCTTAATTCTCATGCCACTGCATTAGAATTAATAAAAGAATCTATAGAACAATCTGGTTATATTTTAAAAGAAGAT
ATTGTCCTAGCAATAGATTGTGCTGCATCTGAATTGTTTGATACATCCACCAATAAGTATAATATAAAAAGTGAAAAAAA
GTTTTTTACTTCTGAAGAATTTACTTATTATTTGTCATCATTAGCGCAAAAATACTCGATAGTTTCTATTGAAGATGGAC
AAAGTGAATACGATTGGGATGGATTTGCTTACCAAACCAAAGTTCTTGGTAATAAAATTCAATTAATAGGAGACGACTTG
TTTGCGACTAACATAAATTTGTTACAAATGGGTATTAATCGAAATATTGCAAATTCTATTTTAATTAAATGCAATCAGAT
AGGATCTTTAACTGAAACACTAGAAACGATTAAAATGGCTAAAAATGCAGGTTACGGTACAATTATTTCACATCGTTCCG
GAGAAACTGAAGATACAAGCATTGCTGACATAGCCGTGGGTACCTCTGCCGGTCAAATAAAAACTGGACCTGTGCGTTGT
TCTGAACGAGTAGCTAAATATAATCAACTAATTCGTATAGAAGAAATGTTAGGAAAAAAAGGAGTGTTTTATAGATTTAA
AAATAAACTTATGTCATAG

Upstream 100 bases:

>100_bases
CATCGTCATAATAAATTAATTTAGTACATACGAGTCAATGTTTATGATATTATTTTTTTGTTCGCATGAAAATCAAGATT
AATTCACAAAGGAAATAAAA

Downstream 100 bases:

>100_bases
TTGATGACTTAATTCAGCATATTAAATATATATATAAAATAGATAAAAGAATATTTTTATAGAAAAATTAAAAGATGAAG
CAATTTGTATGCTTTATACG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPKILNIVGREVIDSRGNPTVETEVYIKGGSRLASVPSGASVGSQEALELRDNNNNRFFGKGVTKAVSFVNGPIHKALIG
MDVTQQRVIDTIMIDLDGTSNKSKFGANAILSVSLAVAKAASAFKNVPLYQHISDLYDAPKNTLVMPLPMMNIINGGKHA
DNNLDIQEFMIIPIGAKTIKEAIQMGSEISYNLRVILSNKGISTQLGDEGGYAPNLNSHATALELIKESIEQSGYILKED
IVLAIDCAASELFDTSTNKYNIKSEKKFFTSEEFTYYLSSLAQKYSIVSIEDGQSEYDWDGFAYQTKVLGNKIQLIGDDL
FATNINLLQMGINRNIANSILIKCNQIGSLTETLETIKMAKNAGYGTIISHRSGETEDTSIADIAVGTSAGQIKTGPVRC
SERVAKYNQLIRIEEMLGKKGVFYRFKNKLMS

Sequences:

>Translated_432_residues
MPKILNIVGREVIDSRGNPTVETEVYIKGGSRLASVPSGASVGSQEALELRDNNNNRFFGKGVTKAVSFVNGPIHKALIG
MDVTQQRVIDTIMIDLDGTSNKSKFGANAILSVSLAVAKAASAFKNVPLYQHISDLYDAPKNTLVMPLPMMNIINGGKHA
DNNLDIQEFMIIPIGAKTIKEAIQMGSEISYNLRVILSNKGISTQLGDEGGYAPNLNSHATALELIKESIEQSGYILKED
IVLAIDCAASELFDTSTNKYNIKSEKKFFTSEEFTYYLSSLAQKYSIVSIEDGQSEYDWDGFAYQTKVLGNKIQLIGDDL
FATNINLLQMGINRNIANSILIKCNQIGSLTETLETIKMAKNAGYGTIISHRSGETEDTSIADIAVGTSAGQIKTGPVRC
SERVAKYNQLIRIEEMLGKKGVFYRFKNKLMS
>Mature_431_residues
PKILNIVGREVIDSRGNPTVETEVYIKGGSRLASVPSGASVGSQEALELRDNNNNRFFGKGVTKAVSFVNGPIHKALIGM
DVTQQRVIDTIMIDLDGTSNKSKFGANAILSVSLAVAKAASAFKNVPLYQHISDLYDAPKNTLVMPLPMMNIINGGKHAD
NNLDIQEFMIIPIGAKTIKEAIQMGSEISYNLRVILSNKGISTQLGDEGGYAPNLNSHATALELIKESIEQSGYILKEDI
VLAIDCAASELFDTSTNKYNIKSEKKFFTSEEFTYYLSSLAQKYSIVSIEDGQSEYDWDGFAYQTKVLGNKIQLIGDDLF
ATNINLLQMGINRNIANSILIKCNQIGSLTETLETIKMAKNAGYGTIISHRSGETEDTSIADIAVGTSAGQIKTGPVRCS
ERVAKYNQLIRIEEMLGKKGVFYRFKNKLMS

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=47.9357798165138, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=46.7741935483871, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=47.6851851851852, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=47.6851851851852, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=43.6194895591647, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI169201331, Length=345, Percent_Identity=28.9855072463768, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI169201757, Length=345, Percent_Identity=28.9855072463768, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI239744207, Length=345, Percent_Identity=28.9855072463768, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=66.1176470588235, Blast_Score=551, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17536383, Length=439, Percent_Identity=47.1526195899772, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71995829, Length=439, Percent_Identity=47.1526195899772, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32563855, Length=195, Percent_Identity=44.6153846153846, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=46.0136674259681, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=46.0136674259681, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=46.0136674259681, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321693, Length=425, Percent_Identity=46.1176470588235, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=46.2790697674419, Blast_Score=345, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580918, Length=420, Percent_Identity=46.9047619047619, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580916, Length=420, Percent_Identity=46.9047619047619, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580920, Length=420, Percent_Identity=46.9047619047619, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580914, Length=420, Percent_Identity=46.9047619047619, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI281360527, Length=420, Percent_Identity=46.9047619047619, Blast_Score=347, Evalue=9e-96,
Organism=Drosophila melanogaster, GI17137654, Length=420, Percent_Identity=46.9047619047619, Blast_Score=347, Evalue=9e-96,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BLOPB (Q493N5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277675.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q493N5
- SMR:   Q493N5
- STRING:   Q493N5
- GeneID:   3563108
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_162
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   CBLO291272:BPEN_162-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47210; Mature: 47078

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 343-343 BINDING 159-159 BINDING 168-168 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKILNIVGREVIDSRGNPTVETEVYIKGGSRLASVPSGASVGSQEALELRDNNNNRFFG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCCCCCCCCCEEEEEECCCCEEEC
KGVTKAVSFVNGPIHKALIGMDVTQQRVIDTIMIDLDGTSNKSKFGANAILSVSLAVAKA
CHHHHHHHHHCCCHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
ASAFKNVPLYQHISDLYDAPKNTLVMPLPMMNIINGGKHADNNLDIQEFMIIPIGAKTIK
HHHHHCCCHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHH
EAIQMGSEISYNLRVILSNKGISTQLGDEGGYAPNLNSHATALELIKESIEQSGYILKED
HHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECC
IVLAIDCAASELFDTSTNKYNIKSEKKFFTSEEFTYYLSSLAQKYSIVSIEDGQSEYDWD
EEEEEECCHHHHHCCCCCCEECCHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCC
GFAYQTKVLGNKIQLIGDDLFATNINLLQMGINRNIANSILIKCNQIGSLTETLETIKMA
CEEEEEHHCCCEEEEEECCHHHCCCEEEEECCCCCCCHHHEEEECCCCCHHHHHHHHHHH
KNAGYGTIISHRSGETEDTSIADIAVGTSAGQIKTGPVRCSERVAKYNQLIRIEEMLGKK
HCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCC
GVFYRFKNKLMS
CHHHHHHHHHCC
>Mature Secondary Structure 
PKILNIVGREVIDSRGNPTVETEVYIKGGSRLASVPSGASVGSQEALELRDNNNNRFFG
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCCCCCCCCCEEEEEECCCCEEEC
KGVTKAVSFVNGPIHKALIGMDVTQQRVIDTIMIDLDGTSNKSKFGANAILSVSLAVAKA
CHHHHHHHHHCCCHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
ASAFKNVPLYQHISDLYDAPKNTLVMPLPMMNIINGGKHADNNLDIQEFMIIPIGAKTIK
HHHHHCCCHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHH
EAIQMGSEISYNLRVILSNKGISTQLGDEGGYAPNLNSHATALELIKESIEQSGYILKED
HHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECC
IVLAIDCAASELFDTSTNKYNIKSEKKFFTSEEFTYYLSSLAQKYSIVSIEDGQSEYDWD
EEEEEECCHHHHHCCCCCCEECCHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCC
GFAYQTKVLGNKIQLIGDDLFATNINLLQMGINRNIANSILIKCNQIGSLTETLETIKMA
CEEEEEHHCCCEEEEEECCHHHCCCEEEEECCCCCCCHHHEEEECCCCCHHHHHHHHHHH
KNAGYGTIISHRSGETEDTSIADIAVGTSAGQIKTGPVRCSERVAKYNQLIRIEEMLGKK
HCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCC
GVFYRFKNKLMS
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA