| Definition | Candidatus Blochmannia pennsylvanicus str. BPEN, complete genome. |
|---|---|
| Accession | NC_007292 |
| Length | 791,654 |
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The map label for this gene is mrcB [H]
Identifier: 71891942
GI number: 71891942
Start: 201636
End: 203972
Strand: Direct
Name: mrcB [H]
Synonym: BPEN_159
Alternate gene names: 71891942
Gene position: 201636-203972 (Clockwise)
Preceding gene: 71891938
Following gene: 71891943
Centisome position: 25.47
GC content: 31.58
Gene sequence:
>2337_bases ATGAATCAATTATCATATAAGTGTCATTCTGTCATTGTGCAACGAGCTTTATTTTTTTTATACACTGTAATGTGCTTTTT TTTCTTTATTATCGCTTTAATTATAGCGTATGGAATATTTTTAGATAAGAAGGTTCAAAGTCGTATTGATGGAAAGGTTT GGAGATTACCTACGATTATTTATAGCCGTATGATTAATGTAGAACCTAATATGTCGTATCATAAAAATGATATAATTCAT TTATTGGAAACTTTACAGTATCGTCAAGTCTCTAAAATTACTCATTCAGGAGAATTTGTTGTACATAATAATGACATTGA ATTATTTCGTAGGCCTTTTAATTTTCCAAGTGGAGAAGAAGGAGAAATGCATGTATCCTTTTTTTTTAATCAAAAAAAAT TACTCCGCATTTATAACCAAGACACAAAGAATAATTTTGGTTTATTTCGATTTGATCCCAAGATCATATCTATTTTATAT GCTCCTAATAAGCAACAAAGATTATTTGTACCGAGATCAGGGTTTCCAGATATATTAATAGATATGTTGTTAGCTGTTGA AGATCGTTATTTTTATCAGCATGATGGTATTCAGATATCTTCTATTGGTCGTGCTTTTTTAGCAAATGTTTTTTCTGGTC GTACTGTACAAGGAGGAAGTACTTTAACGCAACAATTAGTAAAAAATTTATTTTTAAATAATACACGATCATTGTGGCGT AAATTTAATGAAGCCTATATGGCTTTAATTTTCGATCATCGATATAGTAAGGACCGCATTCTTGAATTATATTTGAATGA AATATATTTTGGTCAAAACGGAAATGATCAGATTCATGGATTTCCTTTAGCTAGTTTTTATTATTTTGGTCGCCCGATAA ATGAATTAAGTATAGATCAACAAGCAATGTTAGTGGGGATGATAAAAGGCGCGTCTTTATATAATCCCTGGAAAAATCCA CACATAACACTGGAACGTCGCAATTTAATACTCAAATTGTTAGAACATAGAGATATTATTGATAAAAAAATGTGTGCTAT TCTTAGTGCACGCCCTTTAGGAACGCAGCCAAAGGATGAAATATTAATTTTACAACCTGCTTTTACACAAATGGTAAATA AAGAAATACAAAATATTGATCGAATTAATGATTTTTCTGGAATAAAAATATTTACTACGTTAGATCCTATTTCTCAAAAA GCAGCAGAACAGGCAATGGAATTAGGCATTCATAAATTAAGACATTATTATAACGTAAAAGATTTAGAAGGGGCCATAGT TGTAGTAGATCGATTTAGTGGAAAAATACGAGCTATGGTAGGAGGGTCTGATCCCCATTTTTCTGGATTTAATCGTGCTA TGCATGCTCGTCGTTCTATAGGTTCATTAGCGAAGCCAGCAATTTATTTAGCAGCTTTAAGTCATCCTGATAAATATCAC CTCAATACATGGATTGCTGATGAGCCTATTCATTTACAACAACCAAATGGAGTAATTTGGTCCCCTAAAAATTATGACCG AAAATTTCGAGGCAAAGTCACTCTTATTGATGCTTTTATTAAATCTCTCAATGTACCTACTGTTAATTTAGGTATGACAA TAGGATTAGACGCTATTTCTGATGTTTTAAAAAAATTGGGCATTTCGCCTAATTTTATTTCTTCTTTTCCATCTATCCTT TTAGGATCCATTAGTTTGACACCTATAGAAGTTGCTCAGGAGTTTCAAACTATTGCTAGCGGCGGGCAATATTCTGCCTT ATCATCTGTTTGTTATATTATGAACGAAGAAGATATAGTATTATATCAAAATTTTACCAAGACAGAGCGTGTTATTTTCC CTCAAGCAGCTTATTTAACGTTATATGCAATGCAACAAGTAGCAACACGAGGAACTTCTTGTATTTTATCTTTAAAATTT CCTTGTTCTCAATTAGCCGCAAAAACTGGAACTACTAATGATCTTCGTGATAGTTGGTTTGTTGGAATTGATGACACAGA AGTAACTGTGATTTGGATAGGACGTGACAATAACGGAAGAACTAAGTTAACAGGTACTAATGGCGCATTAACTCTATATA ATTTGTATTTAGATAATAAAAATCCTACTCCTTTACATTTAGAACTACCTAGTGGTATTAAGTACATACCTATAGATAAT TGTGGTAATTTTACACTACATGATAGCAAAAATAATGGTTGTCAGGTTTTGCCAGTGTGGGTGAATGATTGGCAATTATT ATTTCAGTCACTTAAAGAATCAAAAATAATCTCATATAATCAGCAACCAATAAATACTGTAGCAGAACAAGGTATTACAA TACAAATGAATAGATAA
Upstream 100 bases:
>100_bases CATTATGTGACCTACTATCACGTATAATCAAAGGAAAATAAATGATAACCGATTATCAATTTACCTGAGTATTGTAACGT ATATCAATAATATAATTTTT
Downstream 100 bases:
>100_bases ATAATTTTTACTATAAATGATTGTTAATTGTTTGGAAAAACAGCAGTACGATTTATATAATAACGTCCAATGATACTTTA CGTCACTTTTATTTTATTCT
Product: penicillin-binding protein 1b
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 778; Mature: 778
Protein sequence:
>778_residues MNQLSYKCHSVIVQRALFFLYTVMCFFFFIIALIIAYGIFLDKKVQSRIDGKVWRLPTIIYSRMINVEPNMSYHKNDIIH LLETLQYRQVSKITHSGEFVVHNNDIELFRRPFNFPSGEEGEMHVSFFFNQKKLLRIYNQDTKNNFGLFRFDPKIISILY APNKQQRLFVPRSGFPDILIDMLLAVEDRYFYQHDGIQISSIGRAFLANVFSGRTVQGGSTLTQQLVKNLFLNNTRSLWR KFNEAYMALIFDHRYSKDRILELYLNEIYFGQNGNDQIHGFPLASFYYFGRPINELSIDQQAMLVGMIKGASLYNPWKNP HITLERRNLILKLLEHRDIIDKKMCAILSARPLGTQPKDEILILQPAFTQMVNKEIQNIDRINDFSGIKIFTTLDPISQK AAEQAMELGIHKLRHYYNVKDLEGAIVVVDRFSGKIRAMVGGSDPHFSGFNRAMHARRSIGSLAKPAIYLAALSHPDKYH LNTWIADEPIHLQQPNGVIWSPKNYDRKFRGKVTLIDAFIKSLNVPTVNLGMTIGLDAISDVLKKLGISPNFISSFPSIL LGSISLTPIEVAQEFQTIASGGQYSALSSVCYIMNEEDIVLYQNFTKTERVIFPQAAYLTLYAMQQVATRGTSCILSLKF PCSQLAAKTGTTNDLRDSWFVGIDDTEVTVIWIGRDNNGRTKLTGTNGALTLYNLYLDNKNPTPLHLELPSGIKYIPIDN CGNFTLHDSKNNGCQVLPVWVNDWQLLFQSLKESKIISYNQQPINTVAEQGITIQMNR
Sequences:
>Translated_778_residues MNQLSYKCHSVIVQRALFFLYTVMCFFFFIIALIIAYGIFLDKKVQSRIDGKVWRLPTIIYSRMINVEPNMSYHKNDIIH LLETLQYRQVSKITHSGEFVVHNNDIELFRRPFNFPSGEEGEMHVSFFFNQKKLLRIYNQDTKNNFGLFRFDPKIISILY APNKQQRLFVPRSGFPDILIDMLLAVEDRYFYQHDGIQISSIGRAFLANVFSGRTVQGGSTLTQQLVKNLFLNNTRSLWR KFNEAYMALIFDHRYSKDRILELYLNEIYFGQNGNDQIHGFPLASFYYFGRPINELSIDQQAMLVGMIKGASLYNPWKNP HITLERRNLILKLLEHRDIIDKKMCAILSARPLGTQPKDEILILQPAFTQMVNKEIQNIDRINDFSGIKIFTTLDPISQK AAEQAMELGIHKLRHYYNVKDLEGAIVVVDRFSGKIRAMVGGSDPHFSGFNRAMHARRSIGSLAKPAIYLAALSHPDKYH LNTWIADEPIHLQQPNGVIWSPKNYDRKFRGKVTLIDAFIKSLNVPTVNLGMTIGLDAISDVLKKLGISPNFISSFPSIL LGSISLTPIEVAQEFQTIASGGQYSALSSVCYIMNEEDIVLYQNFTKTERVIFPQAAYLTLYAMQQVATRGTSCILSLKF PCSQLAAKTGTTNDLRDSWFVGIDDTEVTVIWIGRDNNGRTKLTGTNGALTLYNLYLDNKNPTPLHLELPSGIKYIPIDN CGNFTLHDSKNNGCQVLPVWVNDWQLLFQSLKESKIISYNQQPINTVAEQGITIQMNR >Mature_778_residues MNQLSYKCHSVIVQRALFFLYTVMCFFFFIIALIIAYGIFLDKKVQSRIDGKVWRLPTIIYSRMINVEPNMSYHKNDIIH LLETLQYRQVSKITHSGEFVVHNNDIELFRRPFNFPSGEEGEMHVSFFFNQKKLLRIYNQDTKNNFGLFRFDPKIISILY APNKQQRLFVPRSGFPDILIDMLLAVEDRYFYQHDGIQISSIGRAFLANVFSGRTVQGGSTLTQQLVKNLFLNNTRSLWR KFNEAYMALIFDHRYSKDRILELYLNEIYFGQNGNDQIHGFPLASFYYFGRPINELSIDQQAMLVGMIKGASLYNPWKNP HITLERRNLILKLLEHRDIIDKKMCAILSARPLGTQPKDEILILQPAFTQMVNKEIQNIDRINDFSGIKIFTTLDPISQK AAEQAMELGIHKLRHYYNVKDLEGAIVVVDRFSGKIRAMVGGSDPHFSGFNRAMHARRSIGSLAKPAIYLAALSHPDKYH LNTWIADEPIHLQQPNGVIWSPKNYDRKFRGKVTLIDAFIKSLNVPTVNLGMTIGLDAISDVLKKLGISPNFISSFPSIL LGSISLTPIEVAQEFQTIASGGQYSALSSVCYIMNEEDIVLYQNFTKTERVIFPQAAYLTLYAMQQVATRGTSCILSLKF PCSQLAAKTGTTNDLRDSWFVGIDDTEVTVIWIGRDNNGRTKLTGTNGALTLYNLYLDNKNPTPLHLELPSGIKYIPIDN CGNFTLHDSKNNGCQVLPVWVNDWQLLFQSLKESKIISYNQQPINTVAEQGITIQMNR
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=735, Percent_Identity=56.734693877551, Blast_Score=874, Evalue=0.0, Organism=Escherichia coli, GI1788867, Length=518, Percent_Identity=26.4478764478764, Blast_Score=150, Evalue=2e-37, Organism=Escherichia coli, GI87082258, Length=259, Percent_Identity=31.6602316602317, Blast_Score=137, Evalue=2e-33, Organism=Escherichia coli, GI1789601, Length=126, Percent_Identity=36.5079365079365, Blast_Score=78, Evalue=2e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 88891; Mature: 88891
Theoretical pI: Translated: 9.28; Mature: 9.28
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNQLSYKCHSVIVQRALFFLYTVMCFFFFIIALIIAYGIFLDKKVQSRIDGKVWRLPTII CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH YSRMINVEPNMSYHKNDIIHLLETLQYRQVSKITHSGEFVVHNNDIELFRRPFNFPSGEE HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCC GEMHVSFFFNQKKLLRIYNQDTKNNFGLFRFDPKIISILYAPNKQQRLFVPRSGFPDILI CCEEEEEEECCHHHHHHHCCCCCCCCCEEEECCEEEEEEECCCCCCEEEECCCCCHHHHH DMLLAVEDRYFYQHDGIQISSIGRAFLANVFSGRTVQGGSTLTQQLVKNLFLNNTRSLWR HHHHHHHHCEEEEECCEEEHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCHHHHHH KFNEAYMALIFDHRYSKDRILELYLNEIYFGQNGNDQIHGFPLASFYYFGRPINELSIDQ HHHHHHEEEEEECCCCHHHHHHHHHHHEEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCH QAMLVGMIKGASLYNPWKNPHITLERRNLILKLLEHRDIIDKKMCAILSARPLGTQPKDE HHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC ILILQPAFTQMVNKEIQNIDRINDFSGIKIFTTLDPISQKAAEQAMELGIHKLRHYYNVK EEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC DLEGAIVVVDRFSGKIRAMVGGSDPHFSGFNRAMHARRSIGSLAKPAIYLAALSHPDKYH CCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE LNTWIADEPIHLQQPNGVIWSPKNYDRKFRGKVTLIDAFIKSLNVPTVNLGMTIGLDAIS EEEEECCCCEEEECCCCEEECCCCCCHHHCCCEEEHHHHHHHCCCCEEEECEEECHHHHH DVLKKLGISPNFISSFPSILLGSISLTPIEVAQEFQTIASGGQYSALSSVCYIMNEEDIV HHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCEE LYQNFTKTERVIFPQAAYLTLYAMQQVATRGTSCILSLKFPCSQLAAKTGTTNDLRDSWF EEECCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCCEE VGIDDTEVTVIWIGRDNNGRTKLTGTNGALTLYNLYLDNKNPTPLHLELPSGIKYIPIDN EEECCCEEEEEEEEECCCCCEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEECC CGNFTLHDSKNNGCQVLPVWVNDWQLLFQSLKESKIISYNQQPINTVAEQGITIQMNR CCCEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCEEECCCCHHHHHHHCCEEEEECC >Mature Secondary Structure MNQLSYKCHSVIVQRALFFLYTVMCFFFFIIALIIAYGIFLDKKVQSRIDGKVWRLPTII CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH YSRMINVEPNMSYHKNDIIHLLETLQYRQVSKITHSGEFVVHNNDIELFRRPFNFPSGEE HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCC GEMHVSFFFNQKKLLRIYNQDTKNNFGLFRFDPKIISILYAPNKQQRLFVPRSGFPDILI CCEEEEEEECCHHHHHHHCCCCCCCCCEEEECCEEEEEEECCCCCCEEEECCCCCHHHHH DMLLAVEDRYFYQHDGIQISSIGRAFLANVFSGRTVQGGSTLTQQLVKNLFLNNTRSLWR HHHHHHHHCEEEEECCEEEHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCHHHHHH KFNEAYMALIFDHRYSKDRILELYLNEIYFGQNGNDQIHGFPLASFYYFGRPINELSIDQ HHHHHHEEEEEECCCCHHHHHHHHHHHEEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCH QAMLVGMIKGASLYNPWKNPHITLERRNLILKLLEHRDIIDKKMCAILSARPLGTQPKDE HHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC ILILQPAFTQMVNKEIQNIDRINDFSGIKIFTTLDPISQKAAEQAMELGIHKLRHYYNVK EEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC DLEGAIVVVDRFSGKIRAMVGGSDPHFSGFNRAMHARRSIGSLAKPAIYLAALSHPDKYH CCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE LNTWIADEPIHLQQPNGVIWSPKNYDRKFRGKVTLIDAFIKSLNVPTVNLGMTIGLDAIS EEEEECCCCEEEECCCCEEECCCCCCHHHCCCEEEHHHHHHHCCCCEEEECEEECHHHHH DVLKKLGISPNFISSFPSILLGSISLTPIEVAQEFQTIASGGQYSALSSVCYIMNEEDIV HHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCEE LYQNFTKTERVIFPQAAYLTLYAMQQVATRGTSCILSLKFPCSQLAAKTGTTNDLRDSWF EEECCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCCEE VGIDDTEVTVIWIGRDNNGRTKLTGTNGALTLYNLYLDNKNPTPLHLELPSGIKYIPIDN EEECCCEEEEEEEEECCCCCEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEECC CGNFTLHDSKNNGCQVLPVWVNDWQLLFQSLKESKIISYNQQPINTVAEQGITIQMNR CCCEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCEEECCCCHHHHHHHCCEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]