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The map label for this gene is holD [H]

Identifier: 71891897

GI number: 71891897

Start: 134912

End: 135334

Strand: Direct

Name: holD [H]

Synonym: BPEN_114

Alternate gene names: 71891897

Gene position: 134912-135334 (Clockwise)

Preceding gene: 71891896

Following gene: 71891898

Centisome position: 17.04

GC content: 30.97

Gene sequence:

>423_bases
ATGCTTACTAATAATAGAAATAAACCACATACGTTTATTTTTCAGGAATTAGGTATTACACTTTGGAAATTACGATATCC
TGTAGTTTTAGACAGCATAAACCCGATTAAGTTATGGCCATCTCTTCGTTTAGTATTAATATCACCGGATGTTCCGATAT
CATTAAATGATCCTTTTATTAAAGATGTTATACGTGCCATGCAGATTAACCCTAGAGAAGTATGCGCGTTGACCACTAAC
CAAATTAAAATTTTAATTATTCCATCAGAGTTTTCTTATTATTGTTGGTGGATTGGTACTAAAGTATCAAATAATTTGAA
TAGTATATGTCTAACTACTCCTGCGTTGTCAATATTAAAATGTGATATTCACGCAAAGCGTGATTTATGGCGACAAATTA
GTGATATTAGACTGAATATTTAA

Upstream 100 bases:

>100_bases
TTATTTTTTAGTACATATGTTAAATGTATGAACATTATTATCAGAATAAATTATTTATATTATTTTCTACTAAATAATGA
TATGTGAAGTATGAATGACT

Downstream 100 bases:

>100_bases
GTTATTTAATCAATATTTTATCGGTGATAAAAAATATATAAAAAATAATACAGTGATATTATCTCGATTTTTTAATTTTT
AAAATTATATGCATACAACT

Product: DNA polymerase III, psi subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLTNNRNKPHTFIFQELGITLWKLRYPVVLDSINPIKLWPSLRLVLISPDVPISLNDPFIKDVIRAMQINPREVCALTTN
QIKILIIPSEFSYYCWWIGTKVSNNLNSICLTTPALSILKCDIHAKRDLWRQISDIRLNI

Sequences:

>Translated_140_residues
MLTNNRNKPHTFIFQELGITLWKLRYPVVLDSINPIKLWPSLRLVLISPDVPISLNDPFIKDVIRAMQINPREVCALTTN
QIKILIIPSEFSYYCWWIGTKVSNNLNSICLTTPALSILKCDIHAKRDLWRQISDIRLNI
>Mature_140_residues
MLTNNRNKPHTFIFQELGITLWKLRYPVVLDSINPIKLWPSLRLVLISPDVPISLNDPFIKDVIRAMQINPREVCALTTN
QIKILIIPSEFSYYCWWIGTKVSNNLNSICLTTPALSILKCDIHAKRDLWRQISDIRLNI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown [H]

COG id: COG3050

COG function: function code L; DNA polymerase III, psi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790831, Length=120, Percent_Identity=35.8333333333333, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004615
- InterPro:   IPR018382
- ProDom:   PD032475 [H]

Pfam domain/function: PF03603 DNA_III_psi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16255; Mature: 16255

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNNRNKPHTFIFQELGITLWKLRYPVVLDSINPIKLWPSLRLVLISPDVPISLNDPFI
CCCCCCCCCCEEEEHHHCCEEEEEECCEEECCCCCEEECCCEEEEEECCCCCEEECCHHH
KDVIRAMQINPREVCALTTNQIKILIIPSEFSYYCWWIGTKVSNNLNSICLTTPALSILK
HHHHHHHCCCCHHEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCEEEEECCCCEEEE
CDIHAKRDLWRQISDIRLNI
ECCCHHHHHHHHHHHEEECC
>Mature Secondary Structure
MLTNNRNKPHTFIFQELGITLWKLRYPVVLDSINPIKLWPSLRLVLISPDVPISLNDPFI
CCCCCCCCCCEEEEHHHCCEEEEEECCEEECCCCCEEECCCEEEEEECCCCCEEECCHHH
KDVIRAMQINPREVCALTTNQIKILIIPSEFSYYCWWIGTKVSNNLNSICLTTPALSILK
HHHHHHHCCCCHHEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCEEEEECCCCEEEE
CDIHAKRDLWRQISDIRLNI
ECCCHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8389364; 8366044; 2828880; 7610040; 9278503; 8505305; 1575709 [H]