Click here to switch to the map view.

The map label for this gene is truB

Identifier: 71891893

GI number: 71891893

Start: 128757

End: 129539

Strand: Direct

Name: truB

Synonym: BPEN_110

Alternate gene names: 71891893

Gene position: 128757-129539 (Clockwise)

Preceding gene: 71891892

Following gene: 71891894

Centisome position: 16.26

GC content: 32.06

Gene sequence:

>783_bases
GTGAAGCGATATAAGAATGTAAGAAATAGAGATATTAATGGCATTTTATTATTAGATAAACCTAAAGGAATATCTTCTGG
TTTATTCTTAAATAAGATCAAAAAATTGTTTAATGCTAAAAAAATAGGACATACTGGTACTTTGGATCCTTTAGCCACAG
GAATGCTGCCTGTTTGTTTTGGTAAAGCGACTAAGCTTGCTAAATACTTGTTACATTCTGATAAACGATATAAAGTCAGC
GCTCAGCTGGGAGTAAGTACTGATACTTTTGATTCAGATGGGACGATTATTAGTGTGTCGCCTGTACAGTCAAATGATTA
TATATTAGAACAATGTTTGGAATCATTTATAGGAATAAGAAATCAAATTCCTCCTATGTTTTCATCATTAAAATATCGTG
GAGTACCTTTATATAAATACGCCAGAAAAGGAATCTATTTTCCTAGAAAACCCAGGTCGATTCATATATACAATCTGAGT
CTTATTAAAAAAACAGAAAATATTATCGAGTTAGATGTCCATTGTTCTACGGGTACTTATATTCGTAGTATAGTGAATGA
TATTGGAGAGTATTTAGGTTGCGGAGCACATGTCATAGAATTACGTCGTTTATCAGTAGGACAGTACATTTCTTCTTCTA
TGATAAATCCAGCAACTTTAGAAGCTATTTTTTACAATGATTCTTTTGATGATGTACAAGTGTTTTGCAAATTGGATGCA
TTCTTGACACCAATGAATATGATCATGCTTGAGCTTGCGAATATATCCAATGAAAAATGTTGA

Upstream 100 bases:

>100_bases
TGATTCTTCATTAATTGAAGGAGCAAAAGTATGTAGTTTAATTTCTACTACAGAGAAATTATGATATAAAAGTTAAATTA
ATAATGCATTAAGGTTTCTA

Downstream 100 bases:

>100_bases
CATTTTCTATATATGTTATTCTTGCGTCAAGAATATAAAATGGATGCATTTTGATAATTGATACAGTCAAATTACGTGAA
CGTATGAGTGTTGATTATAT

Product: tRNA pseudouridine 5S synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKRYKNVRNRDINGILLLDKPKGISSGLFLNKIKKLFNAKKIGHTGTLDPLATGMLPVCFGKATKLAKYLLHSDKRYKVS
AQLGVSTDTFDSDGTIISVSPVQSNDYILEQCLESFIGIRNQIPPMFSSLKYRGVPLYKYARKGIYFPRKPRSIHIYNLS
LIKKTENIIELDVHCSTGTYIRSIVNDIGEYLGCGAHVIELRRLSVGQYISSSMINPATLEAIFYNDSFDDVQVFCKLDA
FLTPMNMIMLELANISNEKC

Sequences:

>Translated_260_residues
MKRYKNVRNRDINGILLLDKPKGISSGLFLNKIKKLFNAKKIGHTGTLDPLATGMLPVCFGKATKLAKYLLHSDKRYKVS
AQLGVSTDTFDSDGTIISVSPVQSNDYILEQCLESFIGIRNQIPPMFSSLKYRGVPLYKYARKGIYFPRKPRSIHIYNLS
LIKKTENIIELDVHCSTGTYIRSIVNDIGEYLGCGAHVIELRRLSVGQYISSSMINPATLEAIFYNDSFDDVQVFCKLDA
FLTPMNMIMLELANISNEKC
>Mature_260_residues
MKRYKNVRNRDINGILLLDKPKGISSGLFLNKIKKLFNAKKIGHTGTLDPLATGMLPVCFGKATKLAKYLLHSDKRYKVS
AQLGVSTDTFDSDGTIISVSPVQSNDYILEQCLESFIGIRNQIPPMFSSLKYRGVPLYKYARKGIYFPRKPRSIHIYNLS
LIKKTENIIELDVHCSTGTYIRSIVNDIGEYLGCGAHVIELRRLSVGQYISSSMINPATLEAIFYNDSFDDVQVFCKLDA
FLTPMNMIMLELANISNEKC

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=218, Percent_Identity=36.2385321100917, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI215599015, Length=196, Percent_Identity=30.6122448979592, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4503337, Length=196, Percent_Identity=30.6122448979592, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI2367200, Length=239, Percent_Identity=51.8828451882845, Blast_Score=259, Evalue=9e-71,
Organism=Caenorhabditis elegans, GI17553978, Length=204, Percent_Identity=30.3921568627451, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=34.0314136125654, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=239, Percent_Identity=27.1966527196653, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364189, Length=206, Percent_Identity=33.0097087378641, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364187, Length=206, Percent_Identity=33.0097087378641, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364185, Length=206, Percent_Identity=33.0097087378641, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364183, Length=206, Percent_Identity=33.0097087378641, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI62471759, Length=206, Percent_Identity=33.0097087378641, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17975520, Length=206, Percent_Identity=33.0097087378641, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BLOPB (Q493T5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277623.1
- ProteinModelPortal:   Q493T5
- SMR:   Q493T5
- STRING:   Q493T5
- GeneID:   3563168
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_110
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2558346
- BioCyc:   CBLO291272:BPEN_110-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 29278; Mature: 29278

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: ACT_SITE 49-49 BINDING 44-44 BINDING 77-77 BINDING 180-180 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYKNVRNRDINGILLLDKPKGISSGLFLNKIKKLFNAKKIGHTGTLDPLATGMLPVCF
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHH
GKATKLAKYLLHSDKRYKVSAQLGVSTDTFDSDGTIISVSPVQSNDYILEQCLESFIGIR
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
NQIPPMFSSLKYRGVPLYKYARKGIYFPRKPRSIHIYNLSLIKKTENIIELDVHCSTGTY
HCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCHH
IRSIVNDIGEYLGCGAHVIELRRLSVGQYISSSMINPATLEAIFYNDSFDDVQVFCKLDA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHHH
FLTPMNMIMLELANISNEKC
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRYKNVRNRDINGILLLDKPKGISSGLFLNKIKKLFNAKKIGHTGTLDPLATGMLPVCF
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHH
GKATKLAKYLLHSDKRYKVSAQLGVSTDTFDSDGTIISVSPVQSNDYILEQCLESFIGIR
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
NQIPPMFSSLKYRGVPLYKYARKGIYFPRKPRSIHIYNLSLIKKTENIIELDVHCSTGTY
HCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCHH
IRSIVNDIGEYLGCGAHVIELRRLSVGQYISSSMINPATLEAIFYNDSFDDVQVFCKLDA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHHH
FLTPMNMIMLELANISNEKC
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA