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The map label for this gene is rrmJ

Identifier: 71891885

GI number: 71891885

Start: 117742

End: 118368

Strand: Direct

Name: rrmJ

Synonym: BPEN_100

Alternate gene names: 71891885

Gene position: 117742-118368 (Clockwise)

Preceding gene: 71891884

Following gene: 71891886

Centisome position: 14.87

GC content: 36.52

Gene sequence:

>627_bases
ATGGTAAATAGCAAATATTCTATGTGTTCTTCAGGATGGTTACAAAAACATTTCAAAGACCAGTATGTGAAAGCAGCACA
AAGACAGAAACTGCGGTCACGGTCTTGGTTTAAGCTTGATGCAATAAATCGCATAGATGCATTGTTTTCTACTGGTATGA
CAGTAATAGATTTGGGATCAGCGCCTGGCGGGTGGGCGCTGTATGTAAAAAGTAAAATAGGAAAGACAGGGCGTATTGTA
GCGTGTGACATATTATCTATGCGTAATATTTCTGGGGTGAATTTTTTGAAAGGAGATTGTTCTAATCCTAATATTTCTAA
AATATTATATAAATGGGTAGGACAGAAACAGGTTAATGTGGTTTTATCTGATATGGCTCCTAATACAACCGGTATATCAA
TAATCGATGTAAGTCGATCTATACATCTTGGGAATTTGGCGTTAAATATATGTCGTAACGTATTAATGCATAGAGGTGCT
TTTTTAGTGAAAGTTTTTCAGGGAAAGGGTTTGGATAAATACTTGTGTGATGTGCATTCCTTATTTAACATAGTAAGGAT
CCGTAAGCCAGATGCCTCTAGGTCTCATTCTCGTGAGGTTTATATTGTAGCGAAAGAACGTAAGTAA

Upstream 100 bases:

>100_bases
AATTTTTATTTTTTTGTTTAAGTAAGATTGAAAGAAATAGAAATTATCGCCATTTTATCAATGTTGTTTATTCAAGAAAA
TATTTGAGAAGTAACCCGAT

Downstream 100 bases:

>100_bases
GCATAATTTATATGTAGATATGTTAAAATTATTTAAAATTTTGTTGTAATGCGAGGTTAATCTTTTGAGTGACATGGCGA
AAAATCTGGGTATCTGGTTA

Product: ribosomal RNA large subunit (23S) methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVNSKYSMCSSGWLQKHFKDQYVKAAQRQKLRSRSWFKLDAINRIDALFSTGMTVIDLGSAPGGWALYVKSKIGKTGRIV
ACDILSMRNISGVNFLKGDCSNPNISKILYKWVGQKQVNVVLSDMAPNTTGISIIDVSRSIHLGNLALNICRNVLMHRGA
FLVKVFQGKGLDKYLCDVHSLFNIVRIRKPDASRSHSREVYIVAKERK

Sequences:

>Translated_208_residues
MVNSKYSMCSSGWLQKHFKDQYVKAAQRQKLRSRSWFKLDAINRIDALFSTGMTVIDLGSAPGGWALYVKSKIGKTGRIV
ACDILSMRNISGVNFLKGDCSNPNISKILYKWVGQKQVNVVLSDMAPNTTGISIIDVSRSIHLGNLALNICRNVLMHRGA
FLVKVFQGKGLDKYLCDVHSLFNIVRIRKPDASRSHSREVYIVAKERK
>Mature_208_residues
MVNSKYSMCSSGWLQKHFKDQYVKAAQRQKLRSRSWFKLDAINRIDALFSTGMTVIDLGSAPGGWALYVKSKIGKTGRIV
ACDILSMRNISGVNFLKGDCSNPNISKILYKWVGQKQVNVVLSDMAPNTTGISIIDVSRSIHLGNLALNICRNVLMHRGA
FLVKVFQGKGLDKYLCDVHSLFNIVRIRKPDASRSHSREVYIVAKERK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=203, Percent_Identity=33.4975369458128, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI194097365, Length=198, Percent_Identity=30.3030303030303, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=55.2884615384615, Blast_Score=249, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17553474, Length=208, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71987550, Length=190, Percent_Identity=28.4210526315789, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71987561, Length=190, Percent_Identity=28.4210526315789, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17553860, Length=190, Percent_Identity=28.4210526315789, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319535, Length=204, Percent_Identity=30.3921568627451, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319796, Length=187, Percent_Identity=28.8770053475936, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859957, Length=190, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356387, Length=211, Percent_Identity=29.8578199052133, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=29.064039408867, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24647580, Length=205, Percent_Identity=27.8048780487805, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_BLOPB (Q493U3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277615.1
- ProteinModelPortal:   Q493U3
- SMR:   Q493U3
- STRING:   Q493U3
- GeneID:   3563181
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_100
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- BioCyc:   CBLO291272:BPEN_100-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23350; Mature: 23350

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: ACT_SITE 164-164 BINDING 63-63 BINDING 65-65 BINDING 83-83 BINDING 99-99 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSKYSMCSSGWLQKHFKDQYVKAAQRQKLRSRSWFKLDAINRIDALFSTGMTVIDLGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCEEEECCC
APGGWALYVKSKIGKTGRIVACDILSMRNISGVNFLKGDCSNPNISKILYKWVGQKQVNV
CCCCEEEEEECCCCCCCCEEEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHCCCCEEEE
VLSDMAPNTTGISIIDVSRSIHLGNLALNICRNVLMHRGAFLVKVFQGKGLDKYLCDVHS
EEECCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
LFNIVRIRKPDASRSHSREVYIVAKERK
HHHHHHCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVNSKYSMCSSGWLQKHFKDQYVKAAQRQKLRSRSWFKLDAINRIDALFSTGMTVIDLGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCEEEECCC
APGGWALYVKSKIGKTGRIVACDILSMRNISGVNFLKGDCSNPNISKILYKWVGQKQVNV
CCCCEEEEEECCCCCCCCEEEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHCCCCEEEE
VLSDMAPNTTGISIIDVSRSIHLGNLALNICRNVLMHRGAFLVKVFQGKGLDKYLCDVHS
EEECCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
LFNIVRIRKPDASRSHSREVYIVAKERK
HHHHHHCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA