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The map label for this gene is greA

Identifier: 71891884

GI number: 71891884

Start: 116889

End: 117365

Strand: Direct

Name: greA

Synonym: BPEN_099

Alternate gene names: 71891884

Gene position: 116889-117365 (Clockwise)

Preceding gene: 71891883

Following gene: 71891885

Centisome position: 14.77

GC content: 33.75

Gene sequence:

>477_bases
ATGAAGCGCATACCAATGACTTTACGTGGAGCTGAAAAGTTAAGAGAGGAACTAGATTATTTAAGAAATGTTCTTCGTCC
AGAAATTATAAAGAATATTTCCGAAGCGCGGGAGCATGGCGATTTAAAAGAAAATGCTGAGTATCATGCTGCTCGAGAAC
AACAAGGATTTTGTGAGGGTCGTATTCAAGAAATAGAGTCTAAACTTTTTAATGCACATATTATAGATATTACCAAATTA
ACTACTGACGATAAAGTAGTTTTTGGCGCTACGGTTGGTGTTGAAAATTTGGATACAACATATAGACAAACGTATAGTAT
TGTAGGGGATGATGAGGCAAATCTTAAGGAACATATGATTTCTATCAATTCTCCTATTGCTAGAGGTTTGATTGGTCATA
AAGAAGGAGATATTGTTCGAATTAATACTCCAAAAGGAAAAGTAAGATATAAAATACTCCAGGTTAAATATTGTTAA

Upstream 100 bases:

>100_bases
TTTTTAAGAAGATAAAATTATAATGTTTAATTAAATCATGCTTTTGTATGTAGCGTGATTGTTTATTGTTTACATGTATG
CAATATAATAAAGGTATAAC

Downstream 100 bases:

>100_bases
ACATATTTTTATTTTCGTTTTTATTTTAAATTTTTTACAAAATGATGATTATGAAAGTAAAAAATAAAATTTTATTCGTA
ATCGTTATATTAATTAATAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKRIPMTLRGAEKLREELDYLRNVLRPEIIKNISEAREHGDLKENAEYHAAREQQGFCEGRIQEIESKLFNAHIIDITKL
TTDDKVVFGATVGVENLDTTYRQTYSIVGDDEANLKEHMISINSPIARGLIGHKEGDIVRINTPKGKVRYKILQVKYC

Sequences:

>Translated_158_residues
MKRIPMTLRGAEKLREELDYLRNVLRPEIIKNISEAREHGDLKENAEYHAAREQQGFCEGRIQEIESKLFNAHIIDITKL
TTDDKVVFGATVGVENLDTTYRQTYSIVGDDEANLKEHMISINSPIARGLIGHKEGDIVRINTPKGKVRYKILQVKYC
>Mature_158_residues
MKRIPMTLRGAEKLREELDYLRNVLRPEIIKNISEAREHGDLKENAEYHAAREQQGFCEGRIQEIESKLFNAHIIDITKL
TTDDKVVFGATVGVENLDTTYRQTYSIVGDDEANLKEHMISINSPIARGLIGHKEGDIVRINTPKGKVRYKILQVKYC

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=67.515923566879, Blast_Score=229, Evalue=9e-62,
Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=36.4779874213836, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BLOPB (Q493U4)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277614.1
- HSSP:   Q8VQD7
- ProteinModelPortal:   Q493U4
- SMR:   Q493U4
- STRING:   Q493U4
- GeneID:   3563180
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_099
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   CBLO291272:BPEN_099-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18095; Mature: 18095

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIPMTLRGAEKLREELDYLRNVLRPEIIKNISEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCHHHH
RIQEIESKLFNAHIIDITKLTTDDKVVFGATVGVENLDTTYRQTYSIVGDDEANLKEHMI
HHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHH
SINSPIARGLIGHKEGDIVRINTPKGKVRYKILQVKYC
HCCCHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure
MKRIPMTLRGAEKLREELDYLRNVLRPEIIKNISEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCHHHH
RIQEIESKLFNAHIIDITKLTTDDKVVFGATVGVENLDTTYRQTYSIVGDDEANLKEHMI
HHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHH
SINSPIARGLIGHKEGDIVRINTPKGKVRYKILQVKYC
HCCCHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA