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The map label for this gene is miaA

Identifier: 71891867

GI number: 71891867

Start: 96505

End: 97419

Strand: Direct

Name: miaA

Synonym: BPEN_081

Alternate gene names: 71891867

Gene position: 96505-97419 (Clockwise)

Preceding gene: 71891866

Following gene: 71891868

Centisome position: 12.19

GC content: 31.69

Gene sequence:

>915_bases
ATGGGTCCTACGGCTTCTGGAAAAACAAGTTTAGCTATAGATTTAAAAAAAAGAAAAGAAAAAATAGATATTATCAGCGT
TGATTCGGCTTTGATATATCGTGATATGGATATCGGTACAGCTAAACCTGTTGCTGAAGAATTAAAATTAGCCCCGCATA
AATTGATAAATATTCGTGATCCAGTTGAATGTTATTCGGCGGCAGATTTTTATCGTGATGCGGATAATGAAATGGAAAAT
ATTATACAGTCTGAACATACACCTTTTTTGGTGGGTGGAACGATGTTATATTTTAAAACTCTGCTAGATGGGTTATTTTT
TTTACCAGCAACTAACCAAAAAATTCGTGATGATCTGGAATATGAAGCAAAAAGAACGGGATGGATAAATATACACGATT
TATTAAAACATATTGATCCGATTTCGGCCAACAAAATTCACTTAAATGATCATAAGAGAATAATCCGAGCATTAGAAATT
TTTTTTATTTCTGGTAAAACATGGACAGAATTAAAATTAATTAATAATCAAAAACTGAAATATCGATTTCATCAATTTGC
TGTTGTTCCGTCAAGTCGTGATTTATTATATAAACGAATTGAGGAACGTTTCCATAGAATGTTAGATATCGGATTTGAAG
ATGAAGTTATCAAATTATTTAATCGATCTGATTTACATACAAAAGAAGTAAAATCGTCCATTTCTTGTGTAGGTTATCGT
CAAATGTGGGAGTATTTATCTGGTGATATTAACTATAATCAGATGATAATTAAAGGAATTTGTGCTACCCGGCAACTTGC
GAAACGACAATTAACCTGGTTGCGTAGATGGCCGAATTTATGCTGGTTAAATAGTGATAATTTATCTGCTGCAACTGATA
GTATATCGAAGATATTAATTGAAAAATCCATCTAA

Upstream 100 bases:

>100_bases
TTTTTGATAAATTATAGAAATGATTTCAGGTAATAACATTTTTGATTTTATATAATTAAGGGAGAATTTTATTAAACATC
AACCATTAGCTATATTTTTA

Downstream 100 bases:

>100_bases
TATATAAAATTTAAAAAATTAAATTTGAAGTTATCTGCTTAATTTAGACCGTTTAGAATAATATAAAATTAATTTTTAAG
GAAATTGAAAATGCACAAAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGPTASGKTSLAIDLKKRKEKIDIISVDSALIYRDMDIGTAKPVAEELKLAPHKLINIRDPVECYSAADFYRDADNEMEN
IIQSEHTPFLVGGTMLYFKTLLDGLFFLPATNQKIRDDLEYEAKRTGWINIHDLLKHIDPISANKIHLNDHKRIIRALEI
FFISGKTWTELKLINNQKLKYRFHQFAVVPSSRDLLYKRIEERFHRMLDIGFEDEVIKLFNRSDLHTKEVKSSISCVGYR
QMWEYLSGDINYNQMIIKGICATRQLAKRQLTWLRRWPNLCWLNSDNLSAATDSISKILIEKSI

Sequences:

>Translated_304_residues
MGPTASGKTSLAIDLKKRKEKIDIISVDSALIYRDMDIGTAKPVAEELKLAPHKLINIRDPVECYSAADFYRDADNEMEN
IIQSEHTPFLVGGTMLYFKTLLDGLFFLPATNQKIRDDLEYEAKRTGWINIHDLLKHIDPISANKIHLNDHKRIIRALEI
FFISGKTWTELKLINNQKLKYRFHQFAVVPSSRDLLYKRIEERFHRMLDIGFEDEVIKLFNRSDLHTKEVKSSISCVGYR
QMWEYLSGDINYNQMIIKGICATRQLAKRQLTWLRRWPNLCWLNSDNLSAATDSISKILIEKSI
>Mature_303_residues
GPTASGKTSLAIDLKKRKEKIDIISVDSALIYRDMDIGTAKPVAEELKLAPHKLINIRDPVECYSAADFYRDADNEMENI
IQSEHTPFLVGGTMLYFKTLLDGLFFLPATNQKIRDDLEYEAKRTGWINIHDLLKHIDPISANKIHLNDHKRIIRALEIF
FISGKTWTELKLINNQKLKYRFHQFAVVPSSRDLLYKRIEERFHRMLDIGFEDEVIKLFNRSDLHTKEVKSSISCVGYRQ
MWEYLSGDINYNQMIIKGICATRQLAKRQLTWLRRWPNLCWLNSDNLSAATDSISKILIEKSI

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=305, Percent_Identity=26.5573770491803, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1790613, Length=299, Percent_Identity=56.5217391304348, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=26.2458471760797, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=28.6713286713287, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24649830, Length=359, Percent_Identity=25.3481894150418, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BLOPB (Q493W0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277596.1
- ProteinModelPortal:   Q493W0
- SMR:   Q493W0
- STRING:   Q493W0
- GeneID:   3563051
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_081
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   CBLO291272:BPEN_081-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35347; Mature: 35216

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTASGKTSLAIDLKKRKEKIDIISVDSALIYRDMDIGTAKPVAEELKLAPHKLINIRD
CCCCCCCCCEEEEEEHHHHCCCCEEEECCEEEEEECCCCCCCHHHHHHHCCHHHHCCCCC
PVECYSAADFYRDADNEMENIIQSEHTPFLVGGTMLYFKTLLDGLFFLPATNQKIRDDLE
HHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHEECCCCCHHHHHHHH
YEAKRTGWINIHDLLKHIDPISANKIHLNDHKRIIRALEIFFISGKTWTELKLINNQKLK
HHHHHCCCEEHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHEECCCCEEEEEEECCCCCE
YRFHQFAVVPSSRDLLYKRIEERFHRMLDIGFEDEVIKLFNRSDLHTKEVKSSISCVGYR
EEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QMWEYLSGDINYNQMIIKGICATRQLAKRQLTWLRRWPNLCWLNSDNLSAATDSISKILI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
EKSI
HCCC
>Mature Secondary Structure 
GPTASGKTSLAIDLKKRKEKIDIISVDSALIYRDMDIGTAKPVAEELKLAPHKLINIRD
CCCCCCCCEEEEEEHHHHCCCCEEEECCEEEEEECCCCCCCHHHHHHHCCHHHHCCCCC
PVECYSAADFYRDADNEMENIIQSEHTPFLVGGTMLYFKTLLDGLFFLPATNQKIRDDLE
HHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHEECCCCCHHHHHHHH
YEAKRTGWINIHDLLKHIDPISANKIHLNDHKRIIRALEIFFISGKTWTELKLINNQKLK
HHHHHCCCEEHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHEECCCCEEEEEEECCCCCE
YRFHQFAVVPSSRDLLYKRIEERFHRMLDIGFEDEVIKLFNRSDLHTKEVKSSISCVGYR
EEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QMWEYLSGDINYNQMIIKGICATRQLAKRQLTWLRRWPNLCWLNSDNLSAATDSISKILI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
EKSI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA