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The map label for this gene is amiB [H]

Identifier: 71891866

GI number: 71891866

Start: 94972

End: 96243

Strand: Direct

Name: amiB [H]

Synonym: BPEN_080

Alternate gene names: 71891866

Gene position: 94972-96243 (Clockwise)

Preceding gene: 71891865

Following gene: 71891867

Centisome position: 12.0

GC content: 30.82

Gene sequence:

>1272_bases
ATGAAGTTAAAATACGAAATAATTTTCGTAATGACAATATTTTCAAGATATTTATGTGTTGAACCAGTTATAGCATCAAC
TCTTAGTTCAATCTCTGTTACCAATAATGCAAATCAAGCTATAGTAACATTAGACAGCGTTGTTGTTCCCGTGTATATGA
TATTTTCCTTACATAACCCGGAAAGAATTGTAATAGATCTTTTAAAAACATCTAAAGTTCAAAAAAATATATTTCCAATA
AATTTCAATGGCACTAATTTAGTCAAATGCATTCGAACAAATACATCTCTTAATCACCAAAGTATACGTATAGTATTAGA
TCTAACTTCTCCTGCAAATATAGGAACAGTAACACAAAAACAAATAAAAGAGCATTATAGTATTATATTAACAATTTTGA
AAAAAGAAATATTTACTATTCCTGAGGTAAATACTCGCAAAATATCACCCATTGTGCATCGTAAAATTCATGTTAATTCA
GGTCAAATGATAAATAATCAAAAGAGAATAATAGATACACGATTTAGAAAAAATCAGAATCACAATAAGACATTATCTCC
TATTATTGTAGCTATCGATGCTGGACATGGAGGGCAAGACCCAGGAGCTACAGGTCGCCATGGAATATATGAAAAAAATA
TTACAATTAATATAGCTAAAAAACTAAAAACATTATTGGATTTAGACCCGAGTTTTAAAGCTGTTATGATCCGAGACGGA
GACTATTTTTTATCGGTCATGGAACGTTCTAATTTGGCTCGAAAAAGAGAAGCAAATGTATTAGTGTCTATTCATGCAGA
TTCTTCTTTAAACACTAATGTTAGAGGGGCATCAGTTTGGGTGTTATCTAATCGTCGTGCTAAAAGTGAAATGATACATT
GGTTACAACGCAGCGAGAAACACGCAGAGTTATTGGGAGGTTTGGGAGATATACTGACTAGTTATCATAATGATCCGTAT
TTTAATCATTTAGTTTTAGACTTGCAATTTGGTTACGCTCAACGAGCAGGGTATGATATTGCAGCACATGTTTTACATCA
ATTAAAAAATATTACTCCTTTACATAAGGATATACCTGAGTATTCTAGTTTTGGTATATTACGATCTCCTGATATCCCTT
CTATTTTAGTAGAGACTGGATTTATTAGTAATGTAAAAGACGAATTTTTATTGGTTAGTAGTGGATATCAAGAAAAAATA
GCTAATGCTTTATACAAAGGCTTACGTTCTTATTTCATAATGTCACAACGAAAATTATTAGATCATTCATAA

Upstream 100 bases:

>100_bases
ATCATGATCATAAAGAATCTAGGCAAGTCATTATAAAATCTTTTAGTAATTTAGGTCACAAAATGTTAGATGCTTTGTTA
GTACGTTGGAAGTTAGACGC

Downstream 100 bases:

>100_bases
TCTTAAAAATATATATTTACTGTAAATTAATAAATAAAAAAGCAACAATTATTATATTAAATAAGTTATTATACATTCAG
TCTTTTTTAGCAATAATGAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKLKYEIIFVMTIFSRYLCVEPVIASTLSSISVTNNANQAIVTLDSVVVPVYMIFSLHNPERIVIDLLKTSKVQKNIFPI
NFNGTNLVKCIRTNTSLNHQSIRIVLDLTSPANIGTVTQKQIKEHYSIILTILKKEIFTIPEVNTRKISPIVHRKIHVNS
GQMINNQKRIIDTRFRKNQNHNKTLSPIIVAIDAGHGGQDPGATGRHGIYEKNITINIAKKLKTLLDLDPSFKAVMIRDG
DYFLSVMERSNLARKREANVLVSIHADSSLNTNVRGASVWVLSNRRAKSEMIHWLQRSEKHAELLGGLGDILTSYHNDPY
FNHLVLDLQFGYAQRAGYDIAAHVLHQLKNITPLHKDIPEYSSFGILRSPDIPSILVETGFISNVKDEFLLVSSGYQEKI
ANALYKGLRSYFIMSQRKLLDHS

Sequences:

>Translated_423_residues
MKLKYEIIFVMTIFSRYLCVEPVIASTLSSISVTNNANQAIVTLDSVVVPVYMIFSLHNPERIVIDLLKTSKVQKNIFPI
NFNGTNLVKCIRTNTSLNHQSIRIVLDLTSPANIGTVTQKQIKEHYSIILTILKKEIFTIPEVNTRKISPIVHRKIHVNS
GQMINNQKRIIDTRFRKNQNHNKTLSPIIVAIDAGHGGQDPGATGRHGIYEKNITINIAKKLKTLLDLDPSFKAVMIRDG
DYFLSVMERSNLARKREANVLVSIHADSSLNTNVRGASVWVLSNRRAKSEMIHWLQRSEKHAELLGGLGDILTSYHNDPY
FNHLVLDLQFGYAQRAGYDIAAHVLHQLKNITPLHKDIPEYSSFGILRSPDIPSILVETGFISNVKDEFLLVSSGYQEKI
ANALYKGLRSYFIMSQRKLLDHS
>Mature_423_residues
MKLKYEIIFVMTIFSRYLCVEPVIASTLSSISVTNNANQAIVTLDSVVVPVYMIFSLHNPERIVIDLLKTSKVQKNIFPI
NFNGTNLVKCIRTNTSLNHQSIRIVLDLTSPANIGTVTQKQIKEHYSIILTILKKEIFTIPEVNTRKISPIVHRKIHVNS
GQMINNQKRIIDTRFRKNQNHNKTLSPIIVAIDAGHGGQDPGATGRHGIYEKNITINIAKKLKTLLDLDPSFKAVMIRDG
DYFLSVMERSNLARKREANVLVSIHADSSLNTNVRGASVWVLSNRRAKSEMIHWLQRSEKHAELLGGLGDILTSYHNDPY
FNHLVLDLQFGYAQRAGYDIAAHVLHQLKNITPLHKDIPEYSSFGILRSPDIPSILVETGFISNVKDEFLLVSSGYQEKI
ANALYKGLRSYFIMSQRKLLDHS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=409, Percent_Identity=47.6772616136919, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI87082163, Length=377, Percent_Identity=28.1167108753316, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=36.1607142857143, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 47817; Mature: 47817

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKYEIIFVMTIFSRYLCVEPVIASTLSSISVTNNANQAIVTLDSVVVPVYMIFSLHNP
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHHHCCCC
ERIVIDLLKTSKVQKNIFPINFNGTNLVKCIRTNTSLNHQSIRIVLDLTSPANIGTVTQK
CCEEHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCHHHH
QIKEHYSIILTILKKEIFTIPEVNTRKISPIVHRKIHVNSGQMINNQKRIIDTRFRKNQN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCCCCEECCCHHHHHHHHHCCCC
HNKTLSPIIVAIDAGHGGQDPGATGRHGIYEKNITINIAKKLKTLLDLDPSFKAVMIRDG
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEHHHHHHHHHCCCCCCEEEEEECC
DYFLSVMERSNLARKREANVLVSIHADSSLNTNVRGASVWVLSNRRAKSEMIHWLQRSEK
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCHH
HAELLGGLGDILTSYHNDPYFNHLVLDLQFGYAQRAGYDIAAHVLHQLKNITPLHKDIPE
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHCCCHHHHHHHHHHHCCCCHHHCCCC
YSSFGILRSPDIPSILVETGFISNVKDEFLLVSSGYQEKIANALYKGLRSYFIMSQRKLL
HHCCCCEECCCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
DHS
CCC
>Mature Secondary Structure
MKLKYEIIFVMTIFSRYLCVEPVIASTLSSISVTNNANQAIVTLDSVVVPVYMIFSLHNP
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHHHCCCC
ERIVIDLLKTSKVQKNIFPINFNGTNLVKCIRTNTSLNHQSIRIVLDLTSPANIGTVTQK
CCEEHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCHHHH
QIKEHYSIILTILKKEIFTIPEVNTRKISPIVHRKIHVNSGQMINNQKRIIDTRFRKNQN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCCCCEECCCHHHHHHHHHCCCC
HNKTLSPIIVAIDAGHGGQDPGATGRHGIYEKNITINIAKKLKTLLDLDPSFKAVMIRDG
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEHHHHHHHHHCCCCCCEEEEEECC
DYFLSVMERSNLARKREANVLVSIHADSSLNTNVRGASVWVLSNRRAKSEMIHWLQRSEK
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCHH
HAELLGGLGDILTSYHNDPYFNHLVLDLQFGYAQRAGYDIAAHVLHQLKNITPLHKDIPE
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHCCCHHHHHHHHHHHCCCCHHHCCCC
YSSFGILRSPDIPSILVETGFISNVKDEFLLVSSGYQEKIANALYKGLRSYFIMSQRKLL
HHCCCCEECCCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
DHS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]