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The map label for this gene is dnaG [H]

Identifier: 71891847

GI number: 71891847

Start: 69956

End: 71701

Strand: Reverse

Name: dnaG [H]

Synonym: BPEN_058

Alternate gene names: 71891847

Gene position: 71701-69956 (Counterclockwise)

Preceding gene: 71891848

Following gene: 71891846

Centisome position: 9.06

GC content: 29.04

Gene sequence:

>1746_bases
ATTACCCGAATCCCAAATACAGTAATTAATGAATTGATATCACGTACTAATATTGTTGATTTAATTAGTCAACGCGTTCC
TTTAAAAAAACAAGGAAAGAATTTTTTAGCCTGTTGTCCATTTCATACTGAAAAACATCCATCATTTACTGTTAATATCG
AAAAACAGTTCTATTATTGCTTCAGCTGTGGATCACATGGAAATGTGATAGATTTTTTGATGCATTATGATCGACTTACA
TTTCTTGAAGCTATCAAAGAACTATCGATCATGCACGGAATCTCAATAGAAAAAAATCTAAATGTTTGTAAGCGCAATAA
TTATCTAAAAAATAACTTATATCAGTTAATGAATAAATTATGCGCATACTATAAAAATATCTTAACCCAAAAAAAATATT
TATACGCTTACAAATATTTGAAAAATCGTGGATTAAATACCAAAACTATTGATGATTTCAATATTGGATTTGCTCCACCC
AGATGGAATAATATAATACAAAAATTTGGTCTTACATTATACGATCAAAAATTACTTGACCAATCAGGTATGTTAATTAG
TAGTAATACAAAAAAATATAAATATGATCGCTTTCGTGACCGATTGATATTTCCTATACGAGATATAACAGGAAGAATTG
TGGCATTCGGAGGTCGTGTTATTACTCAAAACAAAATACCCAAGTATTTAAACTCACCAGATACCGAAATTTTTAAAAAA
AGTCAACATTTATATGGCTTATATGAAGCGCGTATGAAACACAAAAATCTCTCTTGCATACTTTTGGTAGAAGGTTATAT
TGATGTTATCACGTTAACACAATTTGGTATTAATTATGCAGTTGCTGCTTTAGGAACGTCTACTACTGATAATCATATTC
GATTGTTGTATAGTATTACTGATCAAATCATTTGTTGTTATGATGGCGATTATGCTGGTAAAAAAGCTGCTTGGCGTACA
TTAAATACTGCCCTTCCGTATCTTACTGATGAACGTCAAATACATTTCGCATTTTTGCCTCATGGAGAAGATCCAGATAC
ATTAATACGTAAAATAGGAAAAGACAACTTTTTAAAAAGGTTAACTCAAACACAAGATTTATCAAATTTTTTATTTGAAA
CATTATCAAAAAAAACAAATTTACAAACATTATCAGGTCGAGTTAAATTAAGTAGTCTGATATTACCTATACTTAAAAAA
ATTCCAGGGCAAACACTGAAATTATGTTTACTGCAACAATTAGGCAATAAAATAGGGATTTTAGATGATAATAAATTAAG
TCAATTGCTAACAAGAAAACCTGTTACATATACTAATAAAATAAACAGATGTTATGTAAATAACAGCATAGGGCACATAC
TGGTAGGATTATTAATACAAAATCCACGACTTTCTAAATTAGTACCAAGTGTACAAGGATTAGAACAATTGAAACAGAAT
GATATTGTAATGTTTATTGATTTAATTCAAATATGTAAAACATATCCAATATCAACTTCTGCGCAATTATTAGAACATTA
TCGTGGAAATAAATTTTTTTCAAAACTTGAATCGCTAGCATATTGGAATCATTTAATTACAAATGATATGATAGAGACTA
CTTTTGTTGATGCTTTGACCAAATTATATAATCTGACTCTTGAAAAACGCCAAGAGTTACTTATTGCTCGTGATCGTACA
TCTGGTCTAACAACGCAAGAACGTCAAGAATTATGGTTATTAAATCAAACATTATCGAAACATTAA

Upstream 100 bases:

>100_bases
AAAATACCAGGGCACATTTACCTACATATATGACATTCAACCATCCATGATGATAATCATTATGTTATCTGTTCTCAATA
ACATGAGCTAAGGCGAGACA

Downstream 100 bases:

>100_bases
TATAAAAATTACAAACTCTTTGGATTTAATGTCACTAATGAAAACAATCATGAAGATACCTCCTGAATGAAATTATTTTT
GCAATCATTTACTACATATC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MTRIPNTVINELISRTNIVDLISQRVPLKKQGKNFLACCPFHTEKHPSFTVNIEKQFYYCFSCGSHGNVIDFLMHYDRLT
FLEAIKELSIMHGISIEKNLNVCKRNNYLKNNLYQLMNKLCAYYKNILTQKKYLYAYKYLKNRGLNTKTIDDFNIGFAPP
RWNNIIQKFGLTLYDQKLLDQSGMLISSNTKKYKYDRFRDRLIFPIRDITGRIVAFGGRVITQNKIPKYLNSPDTEIFKK
SQHLYGLYEARMKHKNLSCILLVEGYIDVITLTQFGINYAVAALGTSTTDNHIRLLYSITDQIICCYDGDYAGKKAAWRT
LNTALPYLTDERQIHFAFLPHGEDPDTLIRKIGKDNFLKRLTQTQDLSNFLFETLSKKTNLQTLSGRVKLSSLILPILKK
IPGQTLKLCLLQQLGNKIGILDDNKLSQLLTRKPVTYTNKINRCYVNNSIGHILVGLLIQNPRLSKLVPSVQGLEQLKQN
DIVMFIDLIQICKTYPISTSAQLLEHYRGNKFFSKLESLAYWNHLITNDMIETTFVDALTKLYNLTLEKRQELLIARDRT
SGLTTQERQELWLLNQTLSKH

Sequences:

>Translated_581_residues
MTRIPNTVINELISRTNIVDLISQRVPLKKQGKNFLACCPFHTEKHPSFTVNIEKQFYYCFSCGSHGNVIDFLMHYDRLT
FLEAIKELSIMHGISIEKNLNVCKRNNYLKNNLYQLMNKLCAYYKNILTQKKYLYAYKYLKNRGLNTKTIDDFNIGFAPP
RWNNIIQKFGLTLYDQKLLDQSGMLISSNTKKYKYDRFRDRLIFPIRDITGRIVAFGGRVITQNKIPKYLNSPDTEIFKK
SQHLYGLYEARMKHKNLSCILLVEGYIDVITLTQFGINYAVAALGTSTTDNHIRLLYSITDQIICCYDGDYAGKKAAWRT
LNTALPYLTDERQIHFAFLPHGEDPDTLIRKIGKDNFLKRLTQTQDLSNFLFETLSKKTNLQTLSGRVKLSSLILPILKK
IPGQTLKLCLLQQLGNKIGILDDNKLSQLLTRKPVTYTNKINRCYVNNSIGHILVGLLIQNPRLSKLVPSVQGLEQLKQN
DIVMFIDLIQICKTYPISTSAQLLEHYRGNKFFSKLESLAYWNHLITNDMIETTFVDALTKLYNLTLEKRQELLIARDRT
SGLTTQERQELWLLNQTLSKH
>Mature_580_residues
TRIPNTVINELISRTNIVDLISQRVPLKKQGKNFLACCPFHTEKHPSFTVNIEKQFYYCFSCGSHGNVIDFLMHYDRLTF
LEAIKELSIMHGISIEKNLNVCKRNNYLKNNLYQLMNKLCAYYKNILTQKKYLYAYKYLKNRGLNTKTIDDFNIGFAPPR
WNNIIQKFGLTLYDQKLLDQSGMLISSNTKKYKYDRFRDRLIFPIRDITGRIVAFGGRVITQNKIPKYLNSPDTEIFKKS
QHLYGLYEARMKHKNLSCILLVEGYIDVITLTQFGINYAVAALGTSTTDNHIRLLYSITDQIICCYDGDYAGKKAAWRTL
NTALPYLTDERQIHFAFLPHGEDPDTLIRKIGKDNFLKRLTQTQDLSNFLFETLSKKTNLQTLSGRVKLSSLILPILKKI
PGQTLKLCLLQQLGNKIGILDDNKLSQLLTRKPVTYTNKINRCYVNNSIGHILVGLLIQNPRLSKLVPSVQGLEQLKQND
IVMFIDLIQICKTYPISTSAQLLEHYRGNKFFSKLESLAYWNHLITNDMIETTFVDALTKLYNLTLEKRQELLIARDRTS
GLTTQERQELWLLNQTLSKH

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=582, Percent_Identity=50.5154639175258, Blast_Score=619, Evalue=1e-178,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 67262; Mature: 67131

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIPNTVINELISRTNIVDLISQRVPLKKQGKNFLACCPFHTEKHPSFTVNIEKQFYYC
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCCEEEEEEEEEEEE
FSCGSHGNVIDFLMHYDRLTFLEAIKELSIMHGISIEKNLNVCKRNNYLKNNLYQLMNKL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHH
CAYYKNILTQKKYLYAYKYLKNRGLNTKTIDDFNIGFAPPRWNNIIQKFGLTLYDQKLLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHC
QSGMLISSNTKKYKYDRFRDRLIFPIRDITGRIVAFGGRVITQNKIPKYLNSPDTEIFKK
CCCCEEECCCCCHHHHHHHHHEEEEHHHHCCEEEEECCEEEECCCCCHHCCCCCHHHHHH
SQHLYGLYEARMKHKNLSCILLVEGYIDVITLTQFGINYAVAALGTSTTDNHIRLLYSIT
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEC
DQIICCYDGDYAGKKAAWRTLNTALPYLTDERQIHFAFLPHGEDPDTLIRKIGKDNFLKR
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHH
LTQTQDLSNFLFETLSKKTNLQTLSGRVKLSSLILPILKKIPGQTLKLCLLQQLGNKIGI
HHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
LDDNKLSQLLTRKPVTYTNKINRCYVNNSIGHILVGLLIQNPRLSKLVPSVQGLEQLKQN
CCCHHHHHHHHCCCCCHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
DIVMFIDLIQICKTYPISTSAQLLEHYRGNKFFSKLESLAYWNHLITNDMIETTFVDALT
CEEHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLYNLTLEKRQELLIARDRTSGLTTQERQELWLLNQTLSKH
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRIPNTVINELISRTNIVDLISQRVPLKKQGKNFLACCPFHTEKHPSFTVNIEKQFYYC
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCCEEEEEEEEEEEE
FSCGSHGNVIDFLMHYDRLTFLEAIKELSIMHGISIEKNLNVCKRNNYLKNNLYQLMNKL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHH
CAYYKNILTQKKYLYAYKYLKNRGLNTKTIDDFNIGFAPPRWNNIIQKFGLTLYDQKLLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHC
QSGMLISSNTKKYKYDRFRDRLIFPIRDITGRIVAFGGRVITQNKIPKYLNSPDTEIFKK
CCCCEEECCCCCHHHHHHHHHEEEEHHHHCCEEEEECCEEEECCCCCHHCCCCCHHHHHH
SQHLYGLYEARMKHKNLSCILLVEGYIDVITLTQFGINYAVAALGTSTTDNHIRLLYSIT
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEC
DQIICCYDGDYAGKKAAWRTLNTALPYLTDERQIHFAFLPHGEDPDTLIRKIGKDNFLKR
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHH
LTQTQDLSNFLFETLSKKTNLQTLSGRVKLSSLILPILKKIPGQTLKLCLLQQLGNKIGI
HHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
LDDNKLSQLLTRKPVTYTNKINRCYVNNSIGHILVGLLIQNPRLSKLVPSVQGLEQLKQN
CCCHHHHHHHHCCCCCHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
DIVMFIDLIQICKTYPISTSAQLLEHYRGNKFFSKLESLAYWNHLITNDMIETTFVDALT
CEEHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLYNLTLEKRQELLIARDRTSGLTTQERQELWLLNQTLSKH
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]