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The map label for this gene is stp [H]

Identifier: 68537177

GI number: 68537177

Start: 2435862

End: 2437370

Strand: Direct

Name: stp [H]

Synonym: jk2082

Alternate gene names: 68537177

Gene position: 2435862-2437370 (Clockwise)

Preceding gene: 68537173

Following gene: 68537178

Centisome position: 98.92

GC content: 65.41

Gene sequence:

>1509_bases
ATGAACTCCACCCATCCGCAATCCAACAACGCACCATCCGGAACGCCCCGAGGTGCGGCCGACGGAGCTACAACCTCTTC
CACGGCTGCTTCCCCGGATCCGGTCGACTCCCGTCCCGGCGGTATCGGCCTGCTGATGACGGTGCTGATCACCGCGGCCG
TCGCGTTCCAGCTGAACGCCTCCATGCTCTCCCCGGTGCTGGCCACCATGGCTCGCGAGCTGAACACCGACGAGGCCGCC
ACGGGTCTCACCCAGACCGCCTTCTTCACCGGTGGAGCCATGTGCGGCCTGTTCCTGCCGCGGCTGTCGGACATTGTCGG
CCGCCGGAAGATCCTCGTCATCATGCTGGGCGTGATGTCCCTCGGCGGCCTCATCGCCGCGCTGGCGCCCAACATTGGCG
TGCTGATGGTCGCCCGCGCCATGCAGGGCATCGCCGGCCCCACGATCCCCATGACCCTGATCATCCTGCGCAGCCAGGTC
AAGGAGGAAGTCCGCCTGGGCACGCTCATGGGTCTGCTCGCCGCCATCAACGGTGGCGTCGCTGGTATCGACGTGATCCT
GGCCGGCTGGATGGCCGAGCAATGGGGCTTCCGCTCCGTGTTCTGGCTCATCGCCATCATCGGCATCATCGCCACTCTGG
TCGTCGCCTTCGGCGCTCCGGAATCCAAGCCGTCGGACAATGTGAAAATGGACTGGGCGGGCGCTGCTCTGCTGGTGGTA
TCGGTGCTGTGCATCACGTTGGGCGTCAATGAGGCAGGCAAGGCCGGGGCAGCGAACTGGCTGTACGTCATCGCCATGTT
CATTGTCGGTGCGGCCGTCTTCCTAGCCTTCTGGAAGTACGAGAACCGCCACGACCAGCCACTGGTTGCCCCGAAATACC
TGGCCCGCCGCCACACCTGGGCGCTGCTGGCCACCACGGTTCTGACCATGACCGGCGTGTTCGCAGCAGTCAACGGCCTG
GCTATCTCCCTGGCTCAGAACCCGGATGCAGGCTTCGGCCTGGAGGCCGACAAGGCCTCCCTGTGGCTGCTGACCCCCTA
CGCCCTCATTGGTTGGGCCGTAGGACCCTTCGCCGGACGCTGGGCACCGAAGGTGGGCTACGGACGGCTGATCATCATCG
CCAACATCGCCAGCGCGGCTCTCATGCTGGCCTTGGCCTTCGTCGGCGTGCACTCCCTGCCGGTGCTGATCATCACCGTG
ATCCTATTGGGCGTCACCTACGCGGGTATCGGCAACATCGTCATCAACAACCTGGGTGTGGTGAACTCCCCGAAGGATAA
CGAGGGCTTCCTGCCCGGCATGAACTCCGCCGCCTTCAACCTGGGCGCAGGCGTGTCCTTCGCAGTGCTGCCGGTCTTCC
AGGTCGCCGGCTCCCCGCAGGGTTCTGATTCCGTCACGGGTTATGTCACCGCGTTTATCGTCGCAGCGGTGATCCTCGGC
ATCGCCGCGGCCGTGGCGGCGCTGATCCCGAAGCAGACCGTCGCGGAGCTCGACCGCGCCATCGTCTAA

Upstream 100 bases:

>100_bases
TAAGAACCCAATGGTTAGCCCTTATCAATTACCTAACTATGCATTAGAGTGCTAGCTAAACGTTCTGCTGAAGTTTTGAC
GTAACTCACGGGAGCCGACC

Downstream 100 bases:

>100_bases
CCCTCCGGCGCACAATCAACGTCGGGCAGGCGCCCAACCCGCCGGTGCCGCGCGCCAATCCACGTTTCGGCCCCCGCAAG
CCTACGCCCAACCCGCCCAC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNSTHPQSNNAPSGTPRGAADGATTSSTAASPDPVDSRPGGIGLLMTVLITAAVAFQLNASMLSPVLATMARELNTDEAA
TGLTQTAFFTGGAMCGLFLPRLSDIVGRRKILVIMLGVMSLGGLIAALAPNIGVLMVARAMQGIAGPTIPMTLIILRSQV
KEEVRLGTLMGLLAAINGGVAGIDVILAGWMAEQWGFRSVFWLIAIIGIIATLVVAFGAPESKPSDNVKMDWAGAALLVV
SVLCITLGVNEAGKAGAANWLYVIAMFIVGAAVFLAFWKYENRHDQPLVAPKYLARRHTWALLATTVLTMTGVFAAVNGL
AISLAQNPDAGFGLEADKASLWLLTPYALIGWAVGPFAGRWAPKVGYGRLIIIANIASAALMLALAFVGVHSLPVLIITV
ILLGVTYAGIGNIVINNLGVVNSPKDNEGFLPGMNSAAFNLGAGVSFAVLPVFQVAGSPQGSDSVTGYVTAFIVAAVILG
IAAAVAALIPKQTVAELDRAIV

Sequences:

>Translated_502_residues
MNSTHPQSNNAPSGTPRGAADGATTSSTAASPDPVDSRPGGIGLLMTVLITAAVAFQLNASMLSPVLATMARELNTDEAA
TGLTQTAFFTGGAMCGLFLPRLSDIVGRRKILVIMLGVMSLGGLIAALAPNIGVLMVARAMQGIAGPTIPMTLIILRSQV
KEEVRLGTLMGLLAAINGGVAGIDVILAGWMAEQWGFRSVFWLIAIIGIIATLVVAFGAPESKPSDNVKMDWAGAALLVV
SVLCITLGVNEAGKAGAANWLYVIAMFIVGAAVFLAFWKYENRHDQPLVAPKYLARRHTWALLATTVLTMTGVFAAVNGL
AISLAQNPDAGFGLEADKASLWLLTPYALIGWAVGPFAGRWAPKVGYGRLIIIANIASAALMLALAFVGVHSLPVLIITV
ILLGVTYAGIGNIVINNLGVVNSPKDNEGFLPGMNSAAFNLGAGVSFAVLPVFQVAGSPQGSDSVTGYVTAFIVAAVILG
IAAAVAALIPKQTVAELDRAIV
>Mature_502_residues
MNSTHPQSNNAPSGTPRGAADGATTSSTAASPDPVDSRPGGIGLLMTVLITAAVAFQLNASMLSPVLATMARELNTDEAA
TGLTQTAFFTGGAMCGLFLPRLSDIVGRRKILVIMLGVMSLGGLIAALAPNIGVLMVARAMQGIAGPTIPMTLIILRSQV
KEEVRLGTLMGLLAAINGGVAGIDVILAGWMAEQWGFRSVFWLIAIIGIIATLVVAFGAPESKPSDNVKMDWAGAALLVV
SVLCITLGVNEAGKAGAANWLYVIAMFIVGAAVFLAFWKYENRHDQPLVAPKYLARRHTWALLATTVLTMTGVFAAVNGL
AISLAQNPDAGFGLEADKASLWLLTPYALIGWAVGPFAGRWAPKVGYGRLIIIANIASAALMLALAFVGVHSLPVLIITV
ILLGVTYAGIGNIVINNLGVVNSPKDNEGFLPGMNSAAFNLGAGVSFAVLPVFQVAGSPQGSDSVTGYVTAFIVAAVILG
IAAAVAALIPKQTVAELDRAIV

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51761; Mature: 51761

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTHPQSNNAPSGTPRGAADGATTSSTAASPDPVDSRPGGIGLLMTVLITAAVAFQLNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SMLSPVLATMARELNTDEAATGLTQTAFFTGGAMCGLFLPRLSDIVGRRKILVIMLGVMS
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LGGLIAALAPNIGVLMVARAMQGIAGPTIPMTLIILRSQVKEEVRLGTLMGLLAAINGGV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AGIDVILAGWMAEQWGFRSVFWLIAIIGIIATLVVAFGAPESKPSDNVKMDWAGAALLVV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
SVLCITLGVNEAGKAGAANWLYVIAMFIVGAAVFLAFWKYENRHDQPLVAPKYLARRHTW
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALLATTVLTMTGVFAAVNGLAISLAQNPDAGFGLEADKASLWLLTPYALIGWAVGPFAGR
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCC
WAPKVGYGRLIIIANIASAALMLALAFVGVHSLPVLIITVILLGVTYAGIGNIVINNLGV
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VNSPKDNEGFLPGMNSAAFNLGAGVSFAVLPVFQVAGSPQGSDSVTGYVTAFIVAAVILG
CCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IAAAVAALIPKQTVAELDRAIV
HHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MNSTHPQSNNAPSGTPRGAADGATTSSTAASPDPVDSRPGGIGLLMTVLITAAVAFQLNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SMLSPVLATMARELNTDEAATGLTQTAFFTGGAMCGLFLPRLSDIVGRRKILVIMLGVMS
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LGGLIAALAPNIGVLMVARAMQGIAGPTIPMTLIILRSQVKEEVRLGTLMGLLAAINGGV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AGIDVILAGWMAEQWGFRSVFWLIAIIGIIATLVVAFGAPESKPSDNVKMDWAGAALLVV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
SVLCITLGVNEAGKAGAANWLYVIAMFIVGAAVFLAFWKYENRHDQPLVAPKYLARRHTW
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALLATTVLTMTGVFAAVNGLAISLAQNPDAGFGLEADKASLWLLTPYALIGWAVGPFAGR
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCC
WAPKVGYGRLIIIANIASAALMLALAFVGVHSLPVLIITVILLGVTYAGIGNIVINNLGV
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VNSPKDNEGFLPGMNSAAFNLGAGVSFAVLPVFQVAGSPQGSDSVTGYVTAFIVAAVILG
CCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IAAAVAALIPKQTVAELDRAIV
HHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]