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The map label for this gene is rfbE [H]

Identifier: 68537132

GI number: 68537132

Start: 2388040

End: 2388828

Strand: Direct

Name: rfbE [H]

Synonym: jk2037

Alternate gene names: 68537132

Gene position: 2388040-2388828 (Clockwise)

Preceding gene: 68537131

Following gene: 68537133

Centisome position: 96.98

GC content: 62.74

Gene sequence:

>789_bases
ATGGTTTCGATTGACACTTACGACGCTTGCGTAGACTTCCCCATCTTCGATGCGAAGACCCGCTCCATGAAGCAGACATT
CCTGGGGGCTGCCGGCGGCGCGATCGGCAGGAACTCGTCGAATACGGTTATGGTGGAGGCGCTTAAGGGCGTCAATCTGC
ATCTGCAAGATGGCGACCGGATCGGCCTGGTTGGGCACAACGGTGCCGGCAAGTCCACCCTGCTGCGCCTGCTCAGTGGG
ATCTACGAGCCGACGCGCGGGTCCGCGATTGTGCGCGGGCGCGTGGCACCCGTGTTTGACCTGGGTGTGGGCATGGATCC
GGAGATCTCCGGGTACGAGAACATCATGATCCGCGGGCTGTTCCTGGGGCAGACGCGGAAGGCGATGCGGCGGAAAATGG
ACGACATTGCGGAGTTCAGCGAACTTGGGGAATACCTGGATATGCCGCTGCGCACCTACTCGACGGGCATGCGGATTCGG
CTGGCTTTGGGGGTTGTGACCAGCATCGACCCGGAGATTCTGCTGCTGGACGAAGGCATCGGCGCCGTGGATGCTGCGTT
CATGGCTAAGGCGCGCGGGCGACTGGTGGAGATGGTTGAGCGGAGCGGCATCCTGGTTTTCGCCTCGCACAGCAACGAAT
TTTTGGCGCAGCTGTGTGACAGTGCGCTGTGGATCGACCACGGAGAGGTCCGGCAGGTGGGGCGCGTGGATGATGTGGTT
GAGGCCTATGAGGGGCCGGAGGCTGGTGCGCACGTCCGCCAGGTACTGAAGGACCTGGGCCGGGCTTAG

Upstream 100 bases:

>100_bases
CTGACCGTGCTGGGGCTTGCGCTGGCCCTGCTGGTGATGCGCCGCTGGCGCTTCCGCGTGAGCTACTGGGTTTAGGAAGC
TACAGGGTTTAGGAGATGAC

Downstream 100 bases:

>100_bases
CGCCGGTTGGGTGGGCGCCTGCTGGCCTCGCCCCCTCCTTTGTCTACTGCGAATGTCGCTAGAACGGGAGAACGCCAGCT
AAATCAGGCTGTGCAGGGCG

Product: putative ABC transport system, ATP-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MVSIDTYDACVDFPIFDAKTRSMKQTFLGAAGGAIGRNSSNTVMVEALKGVNLHLQDGDRIGLVGHNGAGKSTLLRLLSG
IYEPTRGSAIVRGRVAPVFDLGVGMDPEISGYENIMIRGLFLGQTRKAMRRKMDDIAEFSELGEYLDMPLRTYSTGMRIR
LALGVVTSIDPEILLLDEGIGAVDAAFMAKARGRLVEMVERSGILVFASHSNEFLAQLCDSALWIDHGEVRQVGRVDDVV
EAYEGPEAGAHVRQVLKDLGRA

Sequences:

>Translated_262_residues
MVSIDTYDACVDFPIFDAKTRSMKQTFLGAAGGAIGRNSSNTVMVEALKGVNLHLQDGDRIGLVGHNGAGKSTLLRLLSG
IYEPTRGSAIVRGRVAPVFDLGVGMDPEISGYENIMIRGLFLGQTRKAMRRKMDDIAEFSELGEYLDMPLRTYSTGMRIR
LALGVVTSIDPEILLLDEGIGAVDAAFMAKARGRLVEMVERSGILVFASHSNEFLAQLCDSALWIDHGEVRQVGRVDDVV
EAYEGPEAGAHVRQVLKDLGRA
>Mature_262_residues
MVSIDTYDACVDFPIFDAKTRSMKQTFLGAAGGAIGRNSSNTVMVEALKGVNLHLQDGDRIGLVGHNGAGKSTLLRLLSG
IYEPTRGSAIVRGRVAPVFDLGVGMDPEISGYENIMIRGLFLGQTRKAMRRKMDDIAEFSELGEYLDMPLRTYSTGMRIR
LALGVVTSIDPEILLLDEGIGAVDAAFMAKARGRLVEMVERSGILVFASHSNEFLAQLCDSALWIDHGEVRQVGRVDDVV
EAYEGPEAGAHVRQVLKDLGRA

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=216, Percent_Identity=26.3888888888889, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI31657092, Length=203, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27477115, Length=210, Percent_Identity=27.6190476190476, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI27436953, Length=233, Percent_Identity=24.4635193133047, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI47078218, Length=199, Percent_Identity=25.6281407035176, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI45446740, Length=199, Percent_Identity=25.6281407035176, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI6005701, Length=235, Percent_Identity=24.6808510638298, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI9665248, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790467, Length=209, Percent_Identity=29.6650717703349, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787758, Length=214, Percent_Identity=28.0373831775701, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789032, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081709, Length=215, Percent_Identity=25.1162790697674, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081791, Length=213, Percent_Identity=26.7605633802817, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788761, Length=212, Percent_Identity=26.8867924528302, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081782, Length=214, Percent_Identity=28.0373831775701, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787547, Length=216, Percent_Identity=25, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082267, Length=215, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789962, Length=227, Percent_Identity=24.2290748898678, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787712, Length=207, Percent_Identity=27.0531400966184, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48995001, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789873, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788225, Length=236, Percent_Identity=23.728813559322, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1789891, Length=226, Percent_Identity=26.1061946902655, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789751, Length=233, Percent_Identity=29.1845493562232, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994943, Length=206, Percent_Identity=23.3009708737864, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788450, Length=212, Percent_Identity=28.7735849056604, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786319, Length=197, Percent_Identity=26.3959390862944, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787759, Length=239, Percent_Identity=27.1966527196653, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=215, Percent_Identity=30.2325581395349, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24643674, Length=233, Percent_Identity=26.6094420600858, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007184, Length=184, Percent_Identity=26.6304347826087, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221500365, Length=184, Percent_Identity=26.6304347826087, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28415; Mature: 28415

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIDTYDACVDFPIFDAKTRSMKQTFLGAAGGAIGRNSSNTVMVEALKGVNLHLQDGDR
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEECCCE
IGLVGHNGAGKSTLLRLLSGIYEPTRGSAIVRGRVAPVFDLGVGMDPEISGYENIMIRGL
EEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCEEEEEE
FLGQTRKAMRRKMDDIAEFSELGEYLDMPLRTYSTGMRIRLALGVVTSIDPEILLLDEGI
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEEHHHCCCCCEEEEECCC
GAVDAAFMAKARGRLVEMVERSGILVFASHSNEFLAQLCDSALWIDHGEVRQVGRVDDVV
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHEECCCHHHHHHCHHHHHH
EAYEGPEAGAHVRQVLKDLGRA
HHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVSIDTYDACVDFPIFDAKTRSMKQTFLGAAGGAIGRNSSNTVMVEALKGVNLHLQDGDR
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEECCCE
IGLVGHNGAGKSTLLRLLSGIYEPTRGSAIVRGRVAPVFDLGVGMDPEISGYENIMIRGL
EEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCEEEEEE
FLGQTRKAMRRKMDDIAEFSELGEYLDMPLRTYSTGMRIRLALGVVTSIDPEILLLDEGI
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEEHHHCCCCCEEEEECCC
GAVDAAFMAKARGRLVEMVERSGILVFASHSNEFLAQLCDSALWIDHGEVRQVGRVDDVV
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHEECCCHHHHHHCHHHHHH
EAYEGPEAGAHVRQVLKDLGRA
HHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]