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The map label for this gene is yfhM [H]

Identifier: 68537062

GI number: 68537062

Start: 2294658

End: 2295578

Strand: Direct

Name: yfhM [H]

Synonym: jk1967

Alternate gene names: 68537062

Gene position: 2294658-2295578 (Clockwise)

Preceding gene: 68537061

Following gene: 68537067

Centisome position: 93.18

GC content: 64.28

Gene sequence:

>921_bases
ATGACATTCGAAGATTCTCACGCCGCAGATCCGCACACCCCGCATGCCACTTTCGTGCATTCGCGGGGTGTGCGTCTTCG
CGTGGACGTTCAAGGCCCCCGCAACGCGCCATTGGTACTTCTCATCCACGGTTTCGGCGGCGGAGCTTTCGACTGGCACC
CGCTGATGCGGGAACTCGCGGGTGAGGACCTGCGCCTGGCCGCCGTAGATCTGCGAGGCTATGGCCGTTCGGATAAAACC
CCGCGCGGATACGACCTCACCACCGCCGCCAGCGATATGGCCGGGGTTATCCGCGGCCTGGGTCACACGACCGCCACCGT
AGTTGGGCACGGTTTTGGAGGCATGGTCGCCTGGACTCTGGTGGCTCACAACCCGGAGCGCATCCGCAGCTTCGTCACCC
TTTCCGCCATCAATCCGCTGCTGCGTTTCCGCCGTATCCTGATCCAGCCATTCTCTCAGCCCCACTTCGCTCACCGCCTG
CTGTCTGCCCAGCTGCCGCGCCTTCCGGAGCGCAAGCTGTTGGCCGACAACGCCGCGGCGGCCGAAAAGATCTTTAGGGC
AGGTGTGGCTCCGGGCTTTAGGGACACGGACGCCTACTTCCGCAGCGCCGCACTCCGCCGCGAAGCAATGCAAGTGGATA
AGGTGGCGCATCTTTCCTGTGAGTACCTGCGCTGGCCCTTCCGCAGCCGCTTCCGCCCCGAGGCGATGCGCTTCGAGCGG
ACCTTCCCGGCGACCACACCCGTGCCGCTCTTAGCGGTGGACGGCAGCATGGATCCGATCTACGACGAATCCCTGGCGCA
AAAGTCCGCTCAGAAGGCCGCTACCGTCGAGCACAAGGTACTTTTCGGCGTGGGGCACTACCCACACGTAGAAGATCCAC
CGTGCGTCGCGGAGTTGCTCCGCGAGCACCTGCACGCTTGA

Upstream 100 bases:

>100_bases
GTGATCCCGCAGAAGGGCAAGAACTCTTCCTCCGCGCGCCGCCCGGGAATGTACACCTAAATCCCCCGTGCGCCTCAAGA
TAACTTGAGGATGAATTGAG

Downstream 100 bases:

>100_bases
ATCCCTAGTCCGCCACGCAGGCGCCCGTCGCCGGGCTGCCCTGGTGGGCGGTCACGTCTCCGACGTGCTTCATGACCTCT
TCCCTGGTCAGCGCGTAACC

Product: putative hydrolase

Products: glycol

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTFEDSHAADPHTPHATFVHSRGVRLRVDVQGPRNAPLVLLIHGFGGGAFDWHPLMRELAGEDLRLAAVDLRGYGRSDKT
PRGYDLTTAASDMAGVIRGLGHTTATVVGHGFGGMVAWTLVAHNPERIRSFVTLSAINPLLRFRRILIQPFSQPHFAHRL
LSAQLPRLPERKLLADNAAAAEKIFRAGVAPGFRDTDAYFRSAALRREAMQVDKVAHLSCEYLRWPFRSRFRPEAMRFER
TFPATTPVPLLAVDGSMDPIYDESLAQKSAQKAATVEHKVLFGVGHYPHVEDPPCVAELLREHLHA

Sequences:

>Translated_306_residues
MTFEDSHAADPHTPHATFVHSRGVRLRVDVQGPRNAPLVLLIHGFGGGAFDWHPLMRELAGEDLRLAAVDLRGYGRSDKT
PRGYDLTTAASDMAGVIRGLGHTTATVVGHGFGGMVAWTLVAHNPERIRSFVTLSAINPLLRFRRILIQPFSQPHFAHRL
LSAQLPRLPERKLLADNAAAAEKIFRAGVAPGFRDTDAYFRSAALRREAMQVDKVAHLSCEYLRWPFRSRFRPEAMRFER
TFPATTPVPLLAVDGSMDPIYDESLAQKSAQKAATVEHKVLFGVGHYPHVEDPPCVAELLREHLHA
>Mature_305_residues
TFEDSHAADPHTPHATFVHSRGVRLRVDVQGPRNAPLVLLIHGFGGGAFDWHPLMRELAGEDLRLAAVDLRGYGRSDKTP
RGYDLTTAASDMAGVIRGLGHTTATVVGHGFGGMVAWTLVAHNPERIRSFVTLSAINPLLRFRRILIQPFSQPHFAHRLL
SAQLPRLPERKLLADNAAAAEKIFRAGVAPGFRDTDAYFRSAALRREAMQVDKVAHLSCEYLRWPFRSRFRPEAMRFERT
FPATTPVPLLAVDGSMDPIYDESLAQKSAQKAATVEHKVLFGVGHYPHVEDPPCVAELLREHLHA

Specific function: Not Clear. Seems To Be Implicated In The Early Steps Of Biotin Biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=191, Percent_Identity=27.7486910994764, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI218777840, Length=170, Percent_Identity=34.1176470588235, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI13376164, Length=170, Percent_Identity=34.1176470588235, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI27597073, Length=164, Percent_Identity=27.4390243902439, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554012, Length=316, Percent_Identity=25.9493670886076, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.3.2.3

Molecular weight: Translated: 33980; Mature: 33849

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEDSHAADPHTPHATFVHSRGVRLRVDVQGPRNAPLVLLIHGFGGGAFDWHPLMRELA
CCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHC
GEDLRLAAVDLRGYGRSDKTPRGYDLTTAASDMAGVIRGLGHTTATVVGHGFGGMVAWTL
CCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHH
VAHNPERIRSFVTLSAINPLLRFRRILIQPFSQPHFAHRLLSAQLPRLPERKLLADNAAA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHH
AEKIFRAGVAPGFRDTDAYFRSAALRREAMQVDKVAHLSCEYLRWPFRSRFRPEAMRFER
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHH
TFPATTPVPLLAVDGSMDPIYDESLAQKSAQKAATVEHKVLFGVGHYPHVEDPPCVAELL
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHH
REHLHA
HHHHCC
>Mature Secondary Structure 
TFEDSHAADPHTPHATFVHSRGVRLRVDVQGPRNAPLVLLIHGFGGGAFDWHPLMRELA
CCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHC
GEDLRLAAVDLRGYGRSDKTPRGYDLTTAASDMAGVIRGLGHTTATVVGHGFGGMVAWTL
CCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHH
VAHNPERIRSFVTLSAINPLLRFRRILIQPFSQPHFAHRLLSAQLPRLPERKLLADNAAA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHH
AEKIFRAGVAPGFRDTDAYFRSAALRREAMQVDKVAHLSCEYLRWPFRSRFRPEAMRFER
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHH
TFPATTPVPLLAVDGSMDPIYDESLAQKSAQKAATVEHKVLFGVGHYPHVEDPPCVAELL
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHH
REHLHA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; epoxide

Specific reaction: H2O + an epoxide = a glycol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]