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The map label for this gene is menE [H]

Identifier: 68536970

GI number: 68536970

Start: 2190135

End: 2191499

Strand: Direct

Name: menE [H]

Synonym: jk1876

Alternate gene names: 68536970

Gene position: 2190135-2191499 (Clockwise)

Preceding gene: 68536969

Following gene: 68536971

Centisome position: 88.94

GC content: 67.11

Gene sequence:

>1365_bases
ATGCTGCCCGAGCCGGCTTGTACATTGGTCAGCATGACCGGAAACTCTTCCCTCGTCCCCCTGCAGCCGACCGTCATCAG
CGCGGCCTCGCCCGAGCTTTTCGCCACCTCCCTGGAGGCTCTAAAGCGGCTGCTCGCGGAGGAAATCTCCATCCTCCCCC
TCCCCGCGGACTGGGAACAATCCTGCCCCGACCTGCCGAAACAAATGCGCGCAGGGAGTTCCTTGGCAGACGATCTGGTG
GCGCCAGGCACGCTCATCGCCTGCACCTCTGGCTCCACAGGCACGCCGAAGGGTGCGGTGCTCGGGTCGCGGCAACTCAC
CACTAGTGCGGACGCCACCGCCTCCTTCATCGGGCGCCATTACCAAGCGCAGCCGGGGGCGTGGCTGCTCACACTTCCGG
CCCATCACATCGCAGGACTGCAAGTGATGCTCCGCAGCCTTCGAGCGGGCTACACTCCCCTGGCCGCCGCGCACCTGCAA
GCCCAACACCTACAAGCTCAGCGCGCGCACGCCGGCGCGCGCTTCACCGCAACCGGCTTCATCGCAGACACAGAAGCATT
GGCGGAAACCTACCCGGACGTGGACCTCTACACCTCCCTCGTACCCGCACAGCTGGAAAGAATCGCTGAAGACCCCGCCG
CCGTTAAAGCACTGAAACGCTACGCCTGCGTGCTGGTGGGTGGCGCGGCGGCGCGTGAGGGCGTCATAAAAAAGATGGCC
GAGGAAGGAGCACAGATCGCGCTGACCTACGGCTCCAGCGAGACGGCGGGCGGGATGGTGTACAACGGCCAACCCCTGCC
GGGCGCGCAGGTGCGGATCGACGGCGCGGACGAGCGCGGGGCCGGGCGTGTGCGGATCAGCGGGCCGATGGTGGCGGACG
GGTATCGCAACGTCGATTCGGCGGAGGCGTTTCCGGTGGCAGGTGAGTTCGTGACTTCCGACATCGGAGTTGTGGACGGC
GGCGAAGAGTCAGGCGGCACGCTGCGGATTCTCGGGCGCGCCGACGGGGCCATCAACACCGGCGGCTACAAGGTGCTCCC
AGAAGAGGTGGAAAAGGCCATCTGCCAGCAGGTTCCAGGAGCCGAGGCTGCCTTTACGGCAGGCGTGGAGGATCCCAAGT
ACGCGGAGTTCGGCGAGTCAATCGGGGCGCTGGTAGTACTGCGAGAGGCTGGCAACGGCGCGGCCGAGCACCCCCGCGAA
GTGACCGACCTGGTGCGCGAGGCAATGCGCGGGCAGGCGGGGCCAAGTCTGATTCCCCGGCGCGCCTGGGCTATCCGCGA
GATGCCGACCACTGGGCCGGGCAAGACCGACCGGCAGCAAGCCGCCGCGATGCTGGACGAGCTCTACGGGCAGCCGCACT
CGTAA

Upstream 100 bases:

>100_bases
TAAGCGCCTTCCCGGCCCGGCGGAGCTGGAATCTACCGTGAAGCGAGTCCAGGAGTTCGGCCTAGAGGCCAGCTAACCGC
ACAGCCCCACTGTTCGGAGA

Downstream 100 bases:

>100_bases
ACGCCCCGGCACCTACACACCCCAGCACCTACGAGCTTGGGTCCCGGCGCCTACGGACTAGACTGAAGAGGTTTGCGGCA
CCCCACAGGCGCGAGTGCCG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLPEPACTLVSMTGNSSLVPLQPTVISAASPELFATSLEALKRLLAEEISILPLPADWEQSCPDLPKQMRAGSSLADDLV
APGTLIACTSGSTGTPKGAVLGSRQLTTSADATASFIGRHYQAQPGAWLLTLPAHHIAGLQVMLRSLRAGYTPLAAAHLQ
AQHLQAQRAHAGARFTATGFIADTEALAETYPDVDLYTSLVPAQLERIAEDPAAVKALKRYACVLVGGAAAREGVIKKMA
EEGAQIALTYGSSETAGGMVYNGQPLPGAQVRIDGADERGAGRVRISGPMVADGYRNVDSAEAFPVAGEFVTSDIGVVDG
GEESGGTLRILGRADGAINTGGYKVLPEEVEKAICQQVPGAEAAFTAGVEDPKYAEFGESIGALVVLREAGNGAAEHPRE
VTDLVREAMRGQAGPSLIPRRAWAIREMPTTGPGKTDRQQAAAMLDELYGQPHS

Sequences:

>Translated_454_residues
MLPEPACTLVSMTGNSSLVPLQPTVISAASPELFATSLEALKRLLAEEISILPLPADWEQSCPDLPKQMRAGSSLADDLV
APGTLIACTSGSTGTPKGAVLGSRQLTTSADATASFIGRHYQAQPGAWLLTLPAHHIAGLQVMLRSLRAGYTPLAAAHLQ
AQHLQAQRAHAGARFTATGFIADTEALAETYPDVDLYTSLVPAQLERIAEDPAAVKALKRYACVLVGGAAAREGVIKKMA
EEGAQIALTYGSSETAGGMVYNGQPLPGAQVRIDGADERGAGRVRISGPMVADGYRNVDSAEAFPVAGEFVTSDIGVVDG
GEESGGTLRILGRADGAINTGGYKVLPEEVEKAICQQVPGAEAAFTAGVEDPKYAEFGESIGALVVLREAGNGAAEHPRE
VTDLVREAMRGQAGPSLIPRRAWAIREMPTTGPGKTDRQQAAAMLDELYGQPHS
>Mature_454_residues
MLPEPACTLVSMTGNSSLVPLQPTVISAASPELFATSLEALKRLLAEEISILPLPADWEQSCPDLPKQMRAGSSLADDLV
APGTLIACTSGSTGTPKGAVLGSRQLTTSADATASFIGRHYQAQPGAWLLTLPAHHIAGLQVMLRSLRAGYTPLAAAHLQ
AQHLQAQRAHAGARFTATGFIADTEALAETYPDVDLYTSLVPAQLERIAEDPAAVKALKRYACVLVGGAAAREGVIKKMA
EEGAQIALTYGSSETAGGMVYNGQPLPGAQVRIDGADERGAGRVRISGPMVADGYRNVDSAEAFPVAGEFVTSDIGVVDG
GEESGGTLRILGRADGAINTGGYKVLPEEVEKAICQQVPGAEAAFTAGVEDPKYAEFGESIGALVVLREAGNGAAEHPRE
VTDLVREAMRGQAGPSLIPRRAWAIREMPTTGPGKTDRQQAAAMLDELYGQPHS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=300, Percent_Identity=30.6666666666667, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 47324; Mature: 47324

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPEPACTLVSMTGNSSLVPLQPTVISAASPELFATSLEALKRLLAEEISILPLPADWEQ
CCCCCCEEEEEECCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHHHHCCEECCCCCHHH
SCPDLPKQMRAGSSLADDLVAPGTLIACTSGSTGTPKGAVLGSRQLTTSADATASFIGRH
CCCCHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
YQAQPGAWLLTLPAHHIAGLQVMLRSLRAGYTPLAAAHLQAQHLQAQRAHAGARFTATGF
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
IADTEALAETYPDVDLYTSLVPAQLERIAEDPAAVKALKRYACVLVGGAAAREGVIKKMA
EECHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCHHHHHHHHHHHH
EEGAQIALTYGSSETAGGMVYNGQPLPGAQVRIDGADERGAGRVRISGPMVADGYRNVDS
HCCCEEEEEECCCCCCCCEEECCCCCCCCEEEECCCCCCCCCEEEECCCEEECCCCCCCC
AEAFPVAGEFVTSDIGVVDGGEESGGTLRILGRADGAINTGGYKVLPEEVEKAICQQVPG
CCCCCCCCHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCEECHHHHHHHHHHHCCC
AEAAFTAGVEDPKYAEFGESIGALVVLREAGNGAAEHPREVTDLVREAMRGQAGPSLIPR
CCCEEECCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
RAWAIREMPTTGPGKTDRQQAAAMLDELYGQPHS
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLPEPACTLVSMTGNSSLVPLQPTVISAASPELFATSLEALKRLLAEEISILPLPADWEQ
CCCCCCEEEEEECCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHHHHCCEECCCCCHHH
SCPDLPKQMRAGSSLADDLVAPGTLIACTSGSTGTPKGAVLGSRQLTTSADATASFIGRH
CCCCHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
YQAQPGAWLLTLPAHHIAGLQVMLRSLRAGYTPLAAAHLQAQHLQAQRAHAGARFTATGF
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
IADTEALAETYPDVDLYTSLVPAQLERIAEDPAAVKALKRYACVLVGGAAAREGVIKKMA
EECHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCHHHHHHHHHHHH
EEGAQIALTYGSSETAGGMVYNGQPLPGAQVRIDGADERGAGRVRISGPMVADGYRNVDS
HCCCEEEEEECCCCCCCCEEECCCCCCCCEEEECCCCCCCCCEEEECCCEEECCCCCCCC
AEAFPVAGEFVTSDIGVVDGGEESGGTLRILGRADGAINTGGYKVLPEEVEKAICQQVPG
CCCCCCCCHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCEECHHHHHHHHHHHCCC
AEAAFTAGVEDPKYAEFGESIGALVVLREAGNGAAEHPREVTDLVREAMRGQAGPSLIPR
CCCEEECCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
RAWAIREMPTTGPGKTDRQQAAAMLDELYGQPHS
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]