Click here to switch to the map view.

The map label for this gene is metY [H]

Identifier: 68536784

GI number: 68536784

Start: 1974830

End: 1976161

Strand: Direct

Name: metY [H]

Synonym: jk1694

Alternate gene names: 68536784

Gene position: 1974830-1976161 (Clockwise)

Preceding gene: 68536783

Following gene: 68536785

Centisome position: 80.2

GC content: 65.62

Gene sequence:

>1332_bases
ATGCCGAAGTACAACAACGAGAACGCTGAGCAGTGGGGCTTCGCCACCCGCCAGATCCACGCAGGCCAGCCCGTCGGCGA
TACCGGCGCGCGCAACCTGCCGATCCACTTCACTACCAGCTACGTCTTCAACGACGCAGAGCACGCCAAGCAGCGCTTCC
ACCTGGAAGACCTGGGCCCCATCTACTCCCGCCTGACCAACCCCACCGTCGAGGCACTGGAGAACCGCCTGAACTCCCTG
GAAGGCGGCGTCCACGCAGTCGCTTTCGCCTCCGGCATGGCTGCTGAGACCGCCGCGATCCTCAACCTCGCCGGCGCAGG
CGACCACATCGTCGCCTCCCCGCGCCTCTACGGCGGCACCGAGACCCTGCTGCAGCACACGCTGCCGAAGCTGGGCATCG
AAACCACCTTCGTAGAAAACCCTGACGACCCAGAATCCTGGCAGGCTGCCGTCCGCCCGAACACCAAGGCCTTCTTCGCC
GAGACTTTCGCAAACCCGCAGGCAGACGTGCTGAACATCCCGGCCGTCGCGGAGGTCGCCCACGCTAACCAGGTCCCGCT
GATCGTGGACAACACCCTGGCCACCGCCGCGCTGGTCCGCCCGCTGGAGCTGGGCGCGGACGTCGTGGTTGCATCCCTGA
CGAAGTTCTACACGGGCAACGGCTCCGGCCTGGGCGGCATCATCGTCGACGGCGGCTCCTTCGACTGGTCCGTCGAGCGC
GACGGCCAGCCGGTCTTCCCTGGCTTCGTCAACCCGGATCCCGCCTACCACGGCCTGAAGTACGTGGACCTGGCCCCGGC
TGCCTTCGGCATGAAGCTCCGCGCGGGCATCATCCGCGACACGGGCGCCACCCTCTCCCCGTTCAACGCATGGGTGGTGG
CCCAGGGCCTAGATACCCTGACCCTGCGCATCGCCAAGCACAACGAGAACGCGAAGGCCGTGGCGGAGTTCCTGGCCGAG
CAGCCGCAGGTGGCCACCGTGAACTATGCGGGCCTGCCGACCTCTCCGTGGTTCAAGGTCAAGGAGGAGCTGGGCTTGGA
GTACACCGGCTCTGTTCTGAGCTTCGATCTGAAGGTCGACGACCCGACTTCGGAGGAGGCGAAGGAAAAGGCTTGGGCCT
TCATCGACGCTCTACAGCTGCACTCTGACCTGGCGAATGTCGGCGACGTGCGCTCCCTGGTGGTGCACCCAGCTACGACC
ACCCACTCGCAGTCCGACGCTCATGGTTTGGCCCGCGCAGGTGTGTCCCATGCGACGGTTCGCCTGTCTGTGGGCATCGA
AGACATCACCGACATCATCAACGACCTGAAGCTCGGCTTCGCAGCCATCTAA

Upstream 100 bases:

>100_bases
AAAAAAGTAGACAGAGCGGTTTACTTTTAGCGCGCGTGGGTCTATCGTCGTAAAACAACAAAGCCAAACAAACCAACTAG
CAGCCTAAAGGACCACACCA

Downstream 100 bases:

>100_bases
GGTTGTTAGGTTTCTAGGCTTTAGGCTGATTTCCATGAACCCCGAGTTCCTCCCCGCTTCTGGCGCGTCGCGCACTGTCT
CTATCGGCGACGTTGTCACG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MPKYNNENAEQWGFATRQIHAGQPVGDTGARNLPIHFTTSYVFNDAEHAKQRFHLEDLGPIYSRLTNPTVEALENRLNSL
EGGVHAVAFASGMAAETAAILNLAGAGDHIVASPRLYGGTETLLQHTLPKLGIETTFVENPDDPESWQAAVRPNTKAFFA
ETFANPQADVLNIPAVAEVAHANQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGIIVDGGSFDWSVER
DGQPVFPGFVNPDPAYHGLKYVDLAPAAFGMKLRAGIIRDTGATLSPFNAWVVAQGLDTLTLRIAKHNENAKAVAEFLAE
QPQVATVNYAGLPTSPWFKVKEELGLEYTGSVLSFDLKVDDPTSEEAKEKAWAFIDALQLHSDLANVGDVRSLVVHPATT
THSQSDAHGLARAGVSHATVRLSVGIEDITDIINDLKLGFAAI

Sequences:

>Translated_443_residues
MPKYNNENAEQWGFATRQIHAGQPVGDTGARNLPIHFTTSYVFNDAEHAKQRFHLEDLGPIYSRLTNPTVEALENRLNSL
EGGVHAVAFASGMAAETAAILNLAGAGDHIVASPRLYGGTETLLQHTLPKLGIETTFVENPDDPESWQAAVRPNTKAFFA
ETFANPQADVLNIPAVAEVAHANQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGIIVDGGSFDWSVER
DGQPVFPGFVNPDPAYHGLKYVDLAPAAFGMKLRAGIIRDTGATLSPFNAWVVAQGLDTLTLRIAKHNENAKAVAEFLAE
QPQVATVNYAGLPTSPWFKVKEELGLEYTGSVLSFDLKVDDPTSEEAKEKAWAFIDALQLHSDLANVGDVRSLVVHPATT
THSQSDAHGLARAGVSHATVRLSVGIEDITDIINDLKLGFAAI
>Mature_442_residues
PKYNNENAEQWGFATRQIHAGQPVGDTGARNLPIHFTTSYVFNDAEHAKQRFHLEDLGPIYSRLTNPTVEALENRLNSLE
GGVHAVAFASGMAAETAAILNLAGAGDHIVASPRLYGGTETLLQHTLPKLGIETTFVENPDDPESWQAAVRPNTKAFFAE
TFANPQADVLNIPAVAEVAHANQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGIIVDGGSFDWSVERD
GQPVFPGFVNPDPAYHGLKYVDLAPAAFGMKLRAGIIRDTGATLSPFNAWVVAQGLDTLTLRIAKHNENAKAVAEFLAEQ
PQVATVNYAGLPTSPWFKVKEELGLEYTGSVLSFDLKVDDPTSEEAKEKAWAFIDALQLHSDLANVGDVRSLVVHPATTT
HSQSDAHGLARAGVSHATVRLSVGIEDITDIINDLKLGFAAI

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=415, Percent_Identity=30.3614457831325, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI25453487, Length=414, Percent_Identity=27.2946859903382, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI299473759, Length=415, Percent_Identity=26.2650602409639, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1790375, Length=411, Percent_Identity=29.4403892944039, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789383, Length=435, Percent_Identity=28.735632183908, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=446, Percent_Identity=28.2511210762332, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=32.8571428571429, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=421, Percent_Identity=31.8289786223278, Blast_Score=168, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=439, Percent_Identity=40.5466970387244, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6319307, Length=441, Percent_Identity=31.9727891156463, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=258, Percent_Identity=28.6821705426357, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321904, Length=412, Percent_Identity=24.5145631067961, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922554, Length=430, Percent_Identity=33.0232558139535, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 47354; Mature: 47222

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYNNENAEQWGFATRQIHAGQPVGDTGARNLPIHFTTSYVFNDAEHAKQRFHLEDLGP
CCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEHEEECCHHHHHHHCCHHHHHH
IYSRLTNPTVEALENRLNSLEGGVHAVAFASGMAAETAAILNLAGAGDHIVASPRLYGGT
HHHHHCCHHHHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHEECCCCCCEEECCCCCCCH
ETLLQHTLPKLGIETTFVENPDDPESWQAAVRPNTKAFFAETFANPQADVLNIPAVAEVA
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCHHHHHH
HANQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGIIVDGGSFDWSVER
CCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECC
DGQPVFPGFVNPDPAYHGLKYVDLAPAAFGMKLRAGIIRDTGATLSPFNAWVVAQGLDTL
CCCCCCCCCCCCCCCCCCEEEEEECCHHHCHHHHCCEEECCCCCCCCCCEEEEECCCCEE
TLRIAKHNENAKAVAEFLAEQPQVATVNYAGLPTSPWFKVKEELGLEYTGSVLSFDLKVD
EEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEECCEEEEEEEEC
DPTSEEAKEKAWAFIDALQLHSDLANVGDVRSLVVHPATTTHSQSDAHGLARAGVSHATV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHCCCCEEEE
RLSVGIEDITDIINDLKLGFAAI
EEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKYNNENAEQWGFATRQIHAGQPVGDTGARNLPIHFTTSYVFNDAEHAKQRFHLEDLGP
CCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEHEEECCHHHHHHHCCHHHHHH
IYSRLTNPTVEALENRLNSLEGGVHAVAFASGMAAETAAILNLAGAGDHIVASPRLYGGT
HHHHHCCHHHHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHEECCCCCCEEECCCCCCCH
ETLLQHTLPKLGIETTFVENPDDPESWQAAVRPNTKAFFAETFANPQADVLNIPAVAEVA
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCHHHHHH
HANQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGIIVDGGSFDWSVER
CCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECC
DGQPVFPGFVNPDPAYHGLKYVDLAPAAFGMKLRAGIIRDTGATLSPFNAWVVAQGLDTL
CCCCCCCCCCCCCCCCCCEEEEEECCHHHCHHHHCCEEECCCCCCCCCCEEEEECCCCEE
TLRIAKHNENAKAVAEFLAEQPQVATVNYAGLPTSPWFKVKEELGLEYTGSVLSFDLKVD
EEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEECCEEEEEEEEC
DPTSEEAKEKAWAFIDALQLHSDLANVGDVRSLVVHPATTTHSQSDAHGLARAGVSHATV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHCCCCEEEE
RLSVGIEDITDIINDLKLGFAAI
EEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]