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The map label for this gene is prfC [H]

Identifier: 68536584

GI number: 68536584

Start: 1766448

End: 1768094

Strand: Direct

Name: prfC [H]

Synonym: jk1498

Alternate gene names: 68536584

Gene position: 1766448-1768094 (Clockwise)

Preceding gene: 68536583

Following gene: 68536585

Centisome position: 71.73

GC content: 64.42

Gene sequence:

>1647_bases
ATGAGTACAACCGCATCCGAAGCCTCGCGCCGCCGCACATTCGCCGTCATCGCCCACCCGGATGCCGGTAAGTCCACCCT
CACCGAGGCGCTCGCACTGCACGCCCACATGATCAAAGAAGCCGGCGCCGTGCACGGCAAGGCCGGCCGCAAGTCCACCG
TCTCCGACTGGATGGACATGGAGAAAGACCGTGGCATTTCCATCGCCTCCTCCGCCTTGCAGTTTGAATACGCCCCGGAG
GACCACGACGGCGAGCCCTTCATGATCAACCTGGTTGATACCCCGGGCCACGCGGACTTCTCGGAAGATACCTACCGAGT
TCTTTCCGCCGTGGATGCCGCGGTGATGCTCATTGACGGCGCAAAGGGCCTGGAGCCGCAGACGCTGAAGCTGTTCCGCG
TGTGCAAGGCCCGCGGTCTGCCGATCGTAACCGTCGTGAACAAGTGGGACCGCCCCGGCCGCAGCCCGCTGGAGCTGGTG
GACGAGATCGTCAACGAGATCCAGCTACAACCCACTCCCCTGTTCTGGCCCGTCGGCGAGGCCGGCGACTTCCGCGGATT
GGCGCGTATCAACGACGACGGAGAAGCCGAGGAGTACATCCACTTCCTCCGCACGGCCGGTGGTTCGACGATCGCACCGG
AGGAGCACTACACCCCGGAACAGGCCGTAGAGCGGGAAGAAGACGTATGGGAAACCGCCGCTGAAGAGGTCGAGCTGCTG
GCCGCCGACGGCGCGCTACACGACCAGGAGCTATTCCTGGAGTGCACCACCTCCCCGCTGATCTTCGCCTCCGCGATGCT
GAACTTCGGCGTGCACCAGATTCTGGATACCCTCTGTGCTTTGGCGCCCGCCCCAGCGGGCCGCGATTCCGACCCGAAGG
TGATCGAGGCCGCCGCGGGCGGAAACTCCGTCGCGGTGGACGAGCACCGCGACCCCACCGACGATTTCTCGGGCGTGGTG
TTCAAGGTGCAGGCGGGTATGGACCGCAACCACCGGGACTCCCTGGCTTTCATGCGCGTGGTCTCCGGCGTATTCGAGCG
TGGCATGCAGGTCACGCACGCGCAATCGGGTCGGACTTTCTCTACAAAGTACGCGCTGACCGTCTTCGGCCGCACCCGCG
CCACCGTGGATGCCGCCTACCCCGGCGATATCGTGGGTCTAGTCAACGCTGGTTCGCTCGCCCCGGGCGACACGATTTAC
GCGGGCAAGAAGGTACAGTTCCCGCCAATGCCGCAGTTCGCACCGGAGCACTTCTGCACACTGCGCGCGAAGTCGCTGGG
CAAGTACAAGCAGTTCCGCAAGGCCCTCGAACAGTTGGATTCCGAAGGCGTTGTGCAGATCCTGCGCAACGACGCACGCG
GTGACGCCAACCCGGTCATGGCCGCCGTCGGCCCCATGCAGTTCGAGGTTATGCAGGCTCGAATGGACGTTGAGTACAAC
GTGGAAACCGTGGCCGACCCCGTCCCTTACTCTGTCGCTCGTCGTACTGACGCCGAGTCCGCGCCGGTGCTGGCCAAGCA
GCGAGGCGTGGAGATCTTCACCCGCACAGACGGGGAGCTGATTGCCCTATTCGGCGACAAGTGGAAGCTAGCCTTCGTGG
AGAAGGAGCACCCGGAGCTGACGATGGAGACGCTGGTCGCCGACTAG

Upstream 100 bases:

>100_bases
CGCGAAGGCCTTCGACGAGGCGCTGGCCCAGGCCGCAGACCATAAGAAGAAGACCACGACTACCTGCGCCTAATGCCCAC
CCGCTAGACTTGTGGGCATT

Downstream 100 bases:

>100_bases
GCGGAGTAGCGATTCGCTGCGCCCTCATCAAAAATATTTGCACAAGTCTTGCAACGTTTCGCAGACTGTGCAAATATTTT
GCGCATGGCAAAATTCCTGT

Product: hypothetical protein

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSTTASEASRRRTFAVIAHPDAGKSTLTEALALHAHMIKEAGAVHGKAGRKSTVSDWMDMEKDRGISIASSALQFEYAPE
DHDGEPFMINLVDTPGHADFSEDTYRVLSAVDAAVMLIDGAKGLEPQTLKLFRVCKARGLPIVTVVNKWDRPGRSPLELV
DEIVNEIQLQPTPLFWPVGEAGDFRGLARINDDGEAEEYIHFLRTAGGSTIAPEEHYTPEQAVEREEDVWETAAEEVELL
AADGALHDQELFLECTTSPLIFASAMLNFGVHQILDTLCALAPAPAGRDSDPKVIEAAAGGNSVAVDEHRDPTDDFSGVV
FKVQAGMDRNHRDSLAFMRVVSGVFERGMQVTHAQSGRTFSTKYALTVFGRTRATVDAAYPGDIVGLVNAGSLAPGDTIY
AGKKVQFPPMPQFAPEHFCTLRAKSLGKYKQFRKALEQLDSEGVVQILRNDARGDANPVMAAVGPMQFEVMQARMDVEYN
VETVADPVPYSVARRTDAESAPVLAKQRGVEIFTRTDGELIALFGDKWKLAFVEKEHPELTMETLVAD

Sequences:

>Translated_548_residues
MSTTASEASRRRTFAVIAHPDAGKSTLTEALALHAHMIKEAGAVHGKAGRKSTVSDWMDMEKDRGISIASSALQFEYAPE
DHDGEPFMINLVDTPGHADFSEDTYRVLSAVDAAVMLIDGAKGLEPQTLKLFRVCKARGLPIVTVVNKWDRPGRSPLELV
DEIVNEIQLQPTPLFWPVGEAGDFRGLARINDDGEAEEYIHFLRTAGGSTIAPEEHYTPEQAVEREEDVWETAAEEVELL
AADGALHDQELFLECTTSPLIFASAMLNFGVHQILDTLCALAPAPAGRDSDPKVIEAAAGGNSVAVDEHRDPTDDFSGVV
FKVQAGMDRNHRDSLAFMRVVSGVFERGMQVTHAQSGRTFSTKYALTVFGRTRATVDAAYPGDIVGLVNAGSLAPGDTIY
AGKKVQFPPMPQFAPEHFCTLRAKSLGKYKQFRKALEQLDSEGVVQILRNDARGDANPVMAAVGPMQFEVMQARMDVEYN
VETVADPVPYSVARRTDAESAPVLAKQRGVEIFTRTDGELIALFGDKWKLAFVEKEHPELTMETLVAD
>Mature_547_residues
STTASEASRRRTFAVIAHPDAGKSTLTEALALHAHMIKEAGAVHGKAGRKSTVSDWMDMEKDRGISIASSALQFEYAPED
HDGEPFMINLVDTPGHADFSEDTYRVLSAVDAAVMLIDGAKGLEPQTLKLFRVCKARGLPIVTVVNKWDRPGRSPLELVD
EIVNEIQLQPTPLFWPVGEAGDFRGLARINDDGEAEEYIHFLRTAGGSTIAPEEHYTPEQAVEREEDVWETAAEEVELLA
ADGALHDQELFLECTTSPLIFASAMLNFGVHQILDTLCALAPAPAGRDSDPKVIEAAAGGNSVAVDEHRDPTDDFSGVVF
KVQAGMDRNHRDSLAFMRVVSGVFERGMQVTHAQSGRTFSTKYALTVFGRTRATVDAAYPGDIVGLVNAGSLAPGDTIYA
GKKVQFPPMPQFAPEHFCTLRAKSLGKYKQFRKALEQLDSEGVVQILRNDARGDANPVMAAVGPMQFEVMQARMDVEYNV
ETVADPVPYSVARRTDAESAPVLAKQRGVEIFTRTDGELIALFGDKWKLAFVEKEHPELTMETLVAD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=574, Percent_Identity=25.4355400696864, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI18390331, Length=529, Percent_Identity=23.4404536862004, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=412, Percent_Identity=26.6990291262136, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI25306287, Length=302, Percent_Identity=28.8079470198675, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI157426893, Length=152, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=140, Percent_Identity=33.5714285714286, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=35.5704697986577, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=537, Percent_Identity=40.7821229050279, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1789738, Length=530, Percent_Identity=27.5471698113208, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI48994988, Length=190, Percent_Identity=34.2105263157895, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788922, Length=161, Percent_Identity=34.7826086956522, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=492, Percent_Identity=25, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=512, Percent_Identity=23.828125, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=160, Percent_Identity=31.875, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=32.9192546583851, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=140, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=140, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=492, Percent_Identity=24.5934959349593, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322359, Length=543, Percent_Identity=24.6777163904236, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=31.6455696202532, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=36.875, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=36.875, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=174, Percent_Identity=29.8850574712644, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=497, Percent_Identity=25.7545271629779, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI221458488, Length=549, Percent_Identity=26.4116575591985, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI78706572, Length=161, Percent_Identity=36.0248447204969, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574573, Length=256, Percent_Identity=28.515625, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 59814; Mature: 59683

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTASEASRRRTFAVIAHPDAGKSTLTEALALHAHMIKEAGAVHGKAGRKSTVSDWMDM
CCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EKDRGISIASSALQFEYAPEDHDGEPFMINLVDTPGHADFSEDTYRVLSAVDAAVMLIDG
HHCCCCEEEHHHEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEC
AKGLEPQTLKLFRVCKARGLPIVTVVNKWDRPGRSPLELVDEIVNEIQLQPTPLFWPVGE
CCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
AGDFRGLARINDDGEAEEYIHFLRTAGGSTIAPEEHYTPEQAVEREEDVWETAAEEVELL
CCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
AADGALHDQELFLECTTSPLIFASAMLNFGVHQILDTLCALAPAPAGRDSDPKVIEAAAG
ECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
GNSVAVDEHRDPTDDFSGVVFKVQAGMDRNHRDSLAFMRVVSGVFERGMQVTHAQSGRTF
CCEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEE
STKYALTVFGRTRATVDAAYPGDIVGLVNAGSLAPGDTIYAGKKVQFPPMPQFAPEHFCT
EEEEEEEEECCCCEEEECCCCCCEEEEEECCCCCCCCEEECCCEECCCCCCCCCCHHHHH
LRAKSLGKYKQFRKALEQLDSEGVVQILRNDARGDANPVMAAVGPMQFEVMQARMDVEYN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCEEC
VETVADPVPYSVARRTDAESAPVLAKQRGVEIFTRTDGELIALFGDKWKLAFVEKEHPEL
HHHHCCCCCHHHHHCCCCCCCCCHHHHCCCEEEEECCCCEEEEECCCEEEEEEECCCCCH
TMETLVAD
HHHHHHCC
>Mature Secondary Structure 
STTASEASRRRTFAVIAHPDAGKSTLTEALALHAHMIKEAGAVHGKAGRKSTVSDWMDM
CCCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EKDRGISIASSALQFEYAPEDHDGEPFMINLVDTPGHADFSEDTYRVLSAVDAAVMLIDG
HHCCCCEEEHHHEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEC
AKGLEPQTLKLFRVCKARGLPIVTVVNKWDRPGRSPLELVDEIVNEIQLQPTPLFWPVGE
CCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
AGDFRGLARINDDGEAEEYIHFLRTAGGSTIAPEEHYTPEQAVEREEDVWETAAEEVELL
CCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
AADGALHDQELFLECTTSPLIFASAMLNFGVHQILDTLCALAPAPAGRDSDPKVIEAAAG
ECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
GNSVAVDEHRDPTDDFSGVVFKVQAGMDRNHRDSLAFMRVVSGVFERGMQVTHAQSGRTF
CCEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEE
STKYALTVFGRTRATVDAAYPGDIVGLVNAGSLAPGDTIYAGKKVQFPPMPQFAPEHFCT
EEEEEEEEECCCCEEEECCCCCCEEEEEECCCCCCCCEEECCCEECCCCCCCCCCHHHHH
LRAKSLGKYKQFRKALEQLDSEGVVQILRNDARGDANPVMAAVGPMQFEVMQARMDVEYN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCEEC
VETVADPVPYSVARRTDAESAPVLAKQRGVEIFTRTDGELIALFGDKWKLAFVEKEHPEL
HHHHCCCCCHHHHHCCCCCCCCCHHHHCCCEEEEECCCCEEEEECCCEEEEEEECCCCCH
TMETLVAD
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA