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The map label for this gene is dkgB [H]

Identifier: 68536403

GI number: 68536403

Start: 1561807

End: 1562625

Strand: Direct

Name: dkgB [H]

Synonym: jk1318

Alternate gene names: 68536403

Gene position: 1561807-1562625 (Clockwise)

Preceding gene: 68536402

Following gene: 68536407

Centisome position: 63.42

GC content: 66.67

Gene sequence:

>819_bases
ATGACTGAAACTTCAGCTCTTTCCCTCCCGGAGATCGGGCTCGGCACCTACAAGATGCAGGGCCAGGCGGGCGCGGACTC
CGTGGCTGCGGGCATCCGCGCGGGCTACCGTCTGATCGACACGGCCTACAACTACGAAAACGAAGGCGCGGTGGGCGCGG
GCGTCCGCGCCAGCGGGATCCGCCGCGACGAGGTGATCGTCACCTCCAAGCTGCCCGGCCGCTACCAGCGGCACGACGAC
GCTATCGCTTCCGTCGAGGAATCCCTCTTCCGATTGGGTTTGGACCGCATCGACCTGCATCTCATCCACTGGCCGAATCC
CAAGCAGGGTCACTACGTGGAGGCCTTCGAAACGTTGCTGGAGCTGCGCGAACGGGGGGTAATCCGCCACGTGGGCGTGT
GCAATTTCCTGCCCGAGCACCTGGAGGCCGTCCACGCCGCCACCGGCGAGTACCCCGTGGTCAACCAGATCGAGCTGCAC
CCGCACTTTCCACAGGCCGAGGCCGTCGCGGCACACCGCGAACTTGGCATCGTGACGCAGGCCTGGTCGCCGCTGTTGCG
CGGCGCGGTTCTGGAGGAGCCGGTCATTGCCGAGATTGCTGACGCCCACTCCGCAACCCCCGGGCAGGTCGTCCTAGCGT
GGCACCGCGGCCGCGGGGTGTTGGCGATCCCGAAGGCTGCCTCGGAGGAGCGGCAGCGCGAGAACCTGGAGTCGTTGAAC
GTCACCCTGAGTGATGCAGAAATCGAGGCCATCACGGCGCTGGGCCGTCCGGATGGGCGCCGGAAGAACCAGGATCCGGC
GGTCTACGAGGAGTTCTAG

Upstream 100 bases:

>100_bases
TGATGCAAGCACCAGCGCCGATAGTGTGGACGACTAGACACAGAGTTCTTGTGCAGGCCACGAACAACACAGACCGAACA
GATGCGAAAGGTAGAAACGA

Downstream 100 bases:

>100_bases
CGCGACTGGCGTTCAACCGCGCTCAGTCGCGTTCAGCGGTGCACGCCGCGCTCAGCGGTGCAGGCCGCGTTCAGCGGTGC
AGGCCGCGCTCAGGCGCGCC

Product: putative oxidoreductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTETSALSLPEIGLGTYKMQGQAGADSVAAGIRAGYRLIDTAYNYENEGAVGAGVRASGIRRDEVIVTSKLPGRYQRHDD
AIASVEESLFRLGLDRIDLHLIHWPNPKQGHYVEAFETLLELRERGVIRHVGVCNFLPEHLEAVHAATGEYPVVNQIELH
PHFPQAEAVAAHRELGIVTQAWSPLLRGAVLEEPVIAEIADAHSATPGQVVLAWHRGRGVLAIPKAASEERQRENLESLN
VTLSDAEIEAITALGRPDGRRKNQDPAVYEEF

Sequences:

>Translated_272_residues
MTETSALSLPEIGLGTYKMQGQAGADSVAAGIRAGYRLIDTAYNYENEGAVGAGVRASGIRRDEVIVTSKLPGRYQRHDD
AIASVEESLFRLGLDRIDLHLIHWPNPKQGHYVEAFETLLELRERGVIRHVGVCNFLPEHLEAVHAATGEYPVVNQIELH
PHFPQAEAVAAHRELGIVTQAWSPLLRGAVLEEPVIAEIADAHSATPGQVVLAWHRGRGVLAIPKAASEERQRENLESLN
VTLSDAEIEAITALGRPDGRRKNQDPAVYEEF
>Mature_271_residues
TETSALSLPEIGLGTYKMQGQAGADSVAAGIRAGYRLIDTAYNYENEGAVGAGVRASGIRRDEVIVTSKLPGRYQRHDDA
IASVEESLFRLGLDRIDLHLIHWPNPKQGHYVEAFETLLELRERGVIRHVGVCNFLPEHLEAVHAATGEYPVVNQIELHP
HFPQAEAVAAHRELGIVTQAWSPLLRGAVLEEPVIAEIADAHSATPGQVVLAWHRGRGVLAIPKAASEERQRENLESLNV
TLSDAEIEAITALGRPDGRRKNQDPAVYEEF

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=287, Percent_Identity=31.0104529616725, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI24497577, Length=283, Percent_Identity=30.0353356890459, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI5174391, Length=283, Percent_Identity=30.0353356890459, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI310109922, Length=268, Percent_Identity=31.7164179104478, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI5453543, Length=286, Percent_Identity=30.0699300699301, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI310109920, Length=286, Percent_Identity=30.0699300699301, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI45446745, Length=286, Percent_Identity=30.0699300699301, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI4503285, Length=286, Percent_Identity=30.0699300699301, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=26.4406779661017, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI24497585, Length=286, Percent_Identity=28.6713286713287, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI291291012, Length=265, Percent_Identity=30.5660377358491, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI24497583, Length=286, Percent_Identity=28.3216783216783, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI5174695, Length=288, Percent_Identity=29.8611111111111, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4502049, Length=283, Percent_Identity=29.6819787985866, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI300116273, Length=269, Percent_Identity=30.1115241635688, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI310109924, Length=149, Percent_Identity=30.8724832214765, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI87082198, Length=263, Percent_Identity=36.8821292775665, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1786400, Length=254, Percent_Identity=38.1889763779528, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=32.3420074349442, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788070, Length=309, Percent_Identity=25.5663430420712, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=33.0855018587361, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17564128, Length=284, Percent_Identity=29.5774647887324, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17537075, Length=286, Percent_Identity=31.4685314685315, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17537077, Length=286, Percent_Identity=31.1188811188811, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=266, Percent_Identity=34.2105263157895, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17561300, Length=268, Percent_Identity=32.089552238806, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71998625, Length=267, Percent_Identity=30.7116104868914, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=271, Percent_Identity=26.9372693726937, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537079, Length=293, Percent_Identity=27.3037542662116, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17566692, Length=302, Percent_Identity=29.1390728476821, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17550248, Length=285, Percent_Identity=27.0175438596491, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324694, Length=284, Percent_Identity=35.2112676056338, Blast_Score=171, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6320576, Length=290, Percent_Identity=33.7931034482759, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6322556, Length=264, Percent_Identity=30.6818181818182, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321896, Length=303, Percent_Identity=28.7128712871287, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319625, Length=310, Percent_Identity=29.0322580645161, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320079, Length=232, Percent_Identity=25.4310344827586, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24657054, Length=271, Percent_Identity=33.210332103321, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24663317, Length=274, Percent_Identity=29.5620437956204, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24644950, Length=296, Percent_Identity=28.0405405405405, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21356425, Length=271, Percent_Identity=31.7343173431734, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24662789, Length=282, Percent_Identity=27.3049645390071, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24662785, Length=268, Percent_Identity=32.4626865671642, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20129731, Length=271, Percent_Identity=30.6273062730627, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662781, Length=268, Percent_Identity=32.4626865671642, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI221378297, Length=316, Percent_Identity=25.9493670886076, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45553081, Length=283, Percent_Identity=27.208480565371, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=27.2373540856031, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29805; Mature: 29674

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETSALSLPEIGLGTYKMQGQAGADSVAAGIRAGYRLIDTAYNYENEGAVGAGVRASGI
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCC
RRDEVIVTSKLPGRYQRHDDAIASVEESLFRLGLDRIDLHLIHWPNPKQGHYVEAFETLL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
ELRERGVIRHVGVCNFLPEHLEAVHAATGEYPVVNQIELHPHFPQAEAVAAHRELGIVTQ
HHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
AWSPLLRGAVLEEPVIAEIADAHSATPGQVVLAWHRGRGVLAIPKAASEERQRENLESLN
HHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHHE
VTLSDAEIEAITALGRPDGRRKNQDPAVYEEF
EEEECCCEEEHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TETSALSLPEIGLGTYKMQGQAGADSVAAGIRAGYRLIDTAYNYENEGAVGAGVRASGI
CCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCC
RRDEVIVTSKLPGRYQRHDDAIASVEESLFRLGLDRIDLHLIHWPNPKQGHYVEAFETLL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
ELRERGVIRHVGVCNFLPEHLEAVHAATGEYPVVNQIELHPHFPQAEAVAAHRELGIVTQ
HHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
AWSPLLRGAVLEEPVIAEIADAHSATPGQVVLAWHRGRGVLAIPKAASEERQRENLESLN
HHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHHE
VTLSDAEIEAITALGRPDGRRKNQDPAVYEEF
EEEECCCEEEHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA