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The map label for this gene is ligA

Identifier: 68536402

GI number: 68536402

Start: 1559656

End: 1561743

Strand: Direct

Name: ligA

Synonym: jk1317

Alternate gene names: 68536402

Gene position: 1559656-1561743 (Clockwise)

Preceding gene: 68536401

Following gene: 68536403

Centisome position: 63.34

GC content: 64.46

Gene sequence:

>2088_bases
GTGAGTACTCAATCCCGCTGGCAAGAACTCGCTGACCAGGTGCGCCACCACCGGCAGCTCTACTACTACGGCGAACCGGA
GATCTCGGACGCGGACTTCGACGCCCTGCTGCAGGAGCTCAAAGACCTCGAGCAGGCCCACCCGGAGGTCGTCACCGGCG
CGTCTCCCACCGAGGAAGTCGCGCCCGCCCCGCCCACCAGCAGTCCGTTCCGCAACGTCGACCACCGCGAGCAGATGCTC
AGCCTGGACAACGTCTTTGATACTGAGCAACTCGCCGGCTGGCTGGACCGCACCCCCGCGAAGACATACCTGACGGAACT
GAAGATCGACGGGGCGTCAATAAACCTCCTCTATATCGACGGCCAGCTGGAACTCGCGCTGACCCGCGGCGACGGCACGA
CGGGCGAGGACATCACCCACAACGCGCGGACCCTCGACGACATCCCGGATACCCTCCACGGCACCGATGAATTCCCCGTC
CCCGAACTGGTAGAGATTCGCGGCGAGGTATTCATCGACGTCGAGGACTTTGCCGCCATGAACGCCCAGCGCCAGGCCGA
GGGGCTGAAAATGTTCGCCAACCCTCGCAACGCCGCTGCCGGCGCGATGCGGCAAAAGAATTCCGCGGACACGGCCAAAC
GCCCGCTGAAGTTGATCTGCCACGGTATCGGCGCGCGCGAGGGCTTCTCCCCCGCTTCCCAGCACGATGCGTACCGCGCG
ATTGCCGCGTGGGGGCTGCCGGTTAGCCCGTATACCAAGCAGGTTCACTCTGCCAAGGAGGTCCAGCAGCAGGTCGAGTA
CTGGGGAAACCACCGGCACGACGCGGCGCACGAGATGGACGGGCTGGTCGTCAAGGTCGATTCCCTGGAAGAGCAGCTGG
AGCTGGGCCACACCAGCCGTGCCCCACGCTGGGCGATCGCCTACAAGTACCCGCCGGAGGAGGCGATGACCACGCTGCAC
AACATCCGCGTCGGCATTGGCCGCACGGGTCGCGCCACACCTTATGCCGTCATGGCGCCGAAGTACGTCGCGGGGTCCAC
GGTCTCGATGGCCACGCTGCACAACCCGACGGAGGCCCACCGCAAGGGCGTATTGCTGGGCGATACGATCACCATTCGTA
AGGCCGGCGAGGTCATCCCGGAGGTGTTGGGCCCGGTCGAAGACAAGCGCACGGGCGCGGAGCGGCCTTTCCTATTCTCG
ACGTTCTGCCCCGAGTGCGGCACCCGTTTGGCTCCTTCGAAGGTTGGTGACGCCGACTGGCGTTGCCCCAACACTCAATA
CTGTCCCGGCCAGTTGCATACCCGCTTGACGTACCTGGCTTCCCGCAAGGCGTTTGATATCGACGCTTTGGGCGAAAAGG
GTGCTTATGATCTGATCCATTCCGGTGTGTTGGCCGACGAGTCGGAGCTGTTCGACTTGCGCGCAGAGGATCTGGAAAAG
ACCAGTTCTTATGCGACGAAGGCCGGGAAGCTGAATAAATCTGGCGAGACCCTGCTGGAGAAGCTGCAGTCTGCGAAGGA
GGCGGACCTGTGGCGGGTGCTGGTTGCGCTGTCGATCCGGCACGTGGGTCCGACTGCGGCGAAGGCTTTGGCGAAGCATT
TCGAGTCCGTGGAGGACATCGCCTCTGCTTCCGCGGAGGAGATGGCCGGCATCGACGGTGTGGCCGAGACGATCGCGGAG
TCGATCTCGGATTGGTTCACGGTGGATTGGCACCGTCGGATCGTGGACCGCTGGGCGGCAGCTGGCGTGCGGATGCGCCG
GGAGGCCGGGAACGTTCCCGGTGCGGCAGACGGCGTGGACTCTGCGCTGCTGGAGGGCCTGACGATCGTGGTCACCGGCT
CGTTGGAGGACTTCGACCGGACGGCGGCGAAGGAGGCCATCGAGGCTCGTGGCGGCAAGGCGGCGGGCAGCGTGTCAAAG
AAGACGGACTTCCTGGTGGCGGGCGAAAAGGCTGGCTCCAAGTTGAAGAAGGCTGAGGACCTGGGCGTGCCCGTGCTGGA
TGAGGCAGCCTTCAAGGAACTGCTGGAAAATGGCGCCCCAAGTTCCGGCGATGATGCAAGCACCAGCGCCGATAGTGTGG
ACGACTAG

Upstream 100 bases:

>100_bases
ATCGGCCTGGGAGTCCTAGTCCTCATTGTGCTCGCTGCAGTGGCCGTCTACTTCACCGCCGTCCGCCGCAAGTAGATCCC
CCGCAAGTAGACTAGCGGGG

Downstream 100 bases:

>100_bases
ACACAGAGTTCTTGTGCAGGCCACGAACAACACAGACCGAACAGATGCGAAAGGTAGAAACGAATGACTGAAACTTCAGC
TCTTTCCCTCCCGGAGATCG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSTQSRWQELADQVRHHRQLYYYGEPEISDADFDALLQELKDLEQAHPEVVTGASPTEEVAPAPPTSSPFRNVDHREQML
SLDNVFDTEQLAGWLDRTPAKTYLTELKIDGASINLLYIDGQLELALTRGDGTTGEDITHNARTLDDIPDTLHGTDEFPV
PELVEIRGEVFIDVEDFAAMNAQRQAEGLKMFANPRNAAAGAMRQKNSADTAKRPLKLICHGIGAREGFSPASQHDAYRA
IAAWGLPVSPYTKQVHSAKEVQQQVEYWGNHRHDAAHEMDGLVVKVDSLEEQLELGHTSRAPRWAIAYKYPPEEAMTTLH
NIRVGIGRTGRATPYAVMAPKYVAGSTVSMATLHNPTEAHRKGVLLGDTITIRKAGEVIPEVLGPVEDKRTGAERPFLFS
TFCPECGTRLAPSKVGDADWRCPNTQYCPGQLHTRLTYLASRKAFDIDALGEKGAYDLIHSGVLADESELFDLRAEDLEK
TSSYATKAGKLNKSGETLLEKLQSAKEADLWRVLVALSIRHVGPTAAKALAKHFESVEDIASASAEEMAGIDGVAETIAE
SISDWFTVDWHRRIVDRWAAAGVRMRREAGNVPGAADGVDSALLEGLTIVVTGSLEDFDRTAAKEAIEARGGKAAGSVSK
KTDFLVAGEKAGSKLKKAEDLGVPVLDEAAFKELLENGAPSSGDDASTSADSVDD

Sequences:

>Translated_695_residues
MSTQSRWQELADQVRHHRQLYYYGEPEISDADFDALLQELKDLEQAHPEVVTGASPTEEVAPAPPTSSPFRNVDHREQML
SLDNVFDTEQLAGWLDRTPAKTYLTELKIDGASINLLYIDGQLELALTRGDGTTGEDITHNARTLDDIPDTLHGTDEFPV
PELVEIRGEVFIDVEDFAAMNAQRQAEGLKMFANPRNAAAGAMRQKNSADTAKRPLKLICHGIGAREGFSPASQHDAYRA
IAAWGLPVSPYTKQVHSAKEVQQQVEYWGNHRHDAAHEMDGLVVKVDSLEEQLELGHTSRAPRWAIAYKYPPEEAMTTLH
NIRVGIGRTGRATPYAVMAPKYVAGSTVSMATLHNPTEAHRKGVLLGDTITIRKAGEVIPEVLGPVEDKRTGAERPFLFS
TFCPECGTRLAPSKVGDADWRCPNTQYCPGQLHTRLTYLASRKAFDIDALGEKGAYDLIHSGVLADESELFDLRAEDLEK
TSSYATKAGKLNKSGETLLEKLQSAKEADLWRVLVALSIRHVGPTAAKALAKHFESVEDIASASAEEMAGIDGVAETIAE
SISDWFTVDWHRRIVDRWAAAGVRMRREAGNVPGAADGVDSALLEGLTIVVTGSLEDFDRTAAKEAIEARGGKAAGSVSK
KTDFLVAGEKAGSKLKKAEDLGVPVLDEAAFKELLENGAPSSGDDASTSADSVDD
>Mature_694_residues
STQSRWQELADQVRHHRQLYYYGEPEISDADFDALLQELKDLEQAHPEVVTGASPTEEVAPAPPTSSPFRNVDHREQMLS
LDNVFDTEQLAGWLDRTPAKTYLTELKIDGASINLLYIDGQLELALTRGDGTTGEDITHNARTLDDIPDTLHGTDEFPVP
ELVEIRGEVFIDVEDFAAMNAQRQAEGLKMFANPRNAAAGAMRQKNSADTAKRPLKLICHGIGAREGFSPASQHDAYRAI
AAWGLPVSPYTKQVHSAKEVQQQVEYWGNHRHDAAHEMDGLVVKVDSLEEQLELGHTSRAPRWAIAYKYPPEEAMTTLHN
IRVGIGRTGRATPYAVMAPKYVAGSTVSMATLHNPTEAHRKGVLLGDTITIRKAGEVIPEVLGPVEDKRTGAERPFLFST
FCPECGTRLAPSKVGDADWRCPNTQYCPGQLHTRLTYLASRKAFDIDALGEKGAYDLIHSGVLADESELFDLRAEDLEKT
SSYATKAGKLNKSGETLLEKLQSAKEADLWRVLVALSIRHVGPTAAKALAKHFESVEDIASASAEEMAGIDGVAETIAES
ISDWFTVDWHRRIVDRWAAAGVRMRREAGNVPGAADGVDSALLEGLTIVVTGSLEDFDRTAAKEAIEARGGKAAGSVSKK
TDFLVAGEKAGSKLKKAEDLGVPVLDEAAFKELLENGAPSSGDDASTSADSVDD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=683, Percent_Identity=39.9707174231332, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI87082305, Length=514, Percent_Identity=23.5408560311284, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CORJK (Q4JUL8)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_251107.1
- HSSP:   P26996
- ProteinModelPortal:   Q4JUL8
- STRING:   Q4JUL8
- GeneID:   3433569
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk1317
- NMPDR:   fig|306537.3.peg.2071
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   CJEI306537:JK1317-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75611; Mature: 75480

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 108-108 BINDING 106-106 BINDING 129-129 BINDING 169-169 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQSRWQELADQVRHHRQLYYYGEPEISDADFDALLQELKDLEQAHPEVVTGASPTEEV
CCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHC
APAPPTSSPFRNVDHREQMLSLDNVFDTEQLAGWLDRTPAKTYLTELKIDGASINLLYID
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHEEECCCEEEEEEEC
GQLELALTRGDGTTGEDITHNARTLDDIPDTLHGTDEFPVPELVEIRGEVFIDVEDFAAM
CEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCHHHHCCCCEEEEHHHHHHH
NAQRQAEGLKMFANPRNAAAGAMRQKNSADTAKRPLKLICHGIGAREGFSPASQHDAYRA
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IAAWGLPVSPYTKQVHSAKEVQQQVEYWGNHRHDAAHEMDGLVVKVDSLEEQLELGHTSR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEHHHHHHHHCCCCCC
APRWAIAYKYPPEEAMTTLHNIRVGIGRTGRATPYAVMAPKYVAGSTVSMATLHNPTEAH
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHCCCCEEEHHCCCCHHHH
RKGVLLGDTITIRKAGEVIPEVLGPVEDKRTGAERPFLFSTFCPECGTRLAPSKVGDADW
HCCEEECCEEEEHHHHHHHHHHHCCCHHHCCCCCCCEEEHHHCHHHCCCCCCCCCCCCCC
RCPNTQYCPGQLHTRLTYLASRKAFDIDALGEKGAYDLIHSGVLADESELFDLRAEDLEK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCHHHHHCCHHHHHH
TSSYATKAGKLNKSGETLLEKLQSAKEADLWRVLVALSIRHVGPTAAKALAKHFESVEDI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ASASAEEMAGIDGVAETIAESISDWFTVDWHRRIVDRWAAAGVRMRREAGNVPGAADGVD
HHCCHHHHHCCHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SALLEGLTIVVTGSLEDFDRTAAKEAIEARGGKAAGSVSKKTDFLVAGEKAGSKLKKAED
HHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHCHHHHHHHH
LGVPVLDEAAFKELLENGAPSSGDDASTSADSVDD
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STQSRWQELADQVRHHRQLYYYGEPEISDADFDALLQELKDLEQAHPEVVTGASPTEEV
CCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHC
APAPPTSSPFRNVDHREQMLSLDNVFDTEQLAGWLDRTPAKTYLTELKIDGASINLLYID
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHEEECCCEEEEEEEC
GQLELALTRGDGTTGEDITHNARTLDDIPDTLHGTDEFPVPELVEIRGEVFIDVEDFAAM
CEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCHHHHCCCCEEEEHHHHHHH
NAQRQAEGLKMFANPRNAAAGAMRQKNSADTAKRPLKLICHGIGAREGFSPASQHDAYRA
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IAAWGLPVSPYTKQVHSAKEVQQQVEYWGNHRHDAAHEMDGLVVKVDSLEEQLELGHTSR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEHHHHHHHHCCCCCC
APRWAIAYKYPPEEAMTTLHNIRVGIGRTGRATPYAVMAPKYVAGSTVSMATLHNPTEAH
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHCCCCEEEHHCCCCHHHH
RKGVLLGDTITIRKAGEVIPEVLGPVEDKRTGAERPFLFSTFCPECGTRLAPSKVGDADW
HCCEEECCEEEEHHHHHHHHHHHCCCHHHCCCCCCCEEEHHHCHHHCCCCCCCCCCCCCC
RCPNTQYCPGQLHTRLTYLASRKAFDIDALGEKGAYDLIHSGVLADESELFDLRAEDLEK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCHHHHHCCHHHHHH
TSSYATKAGKLNKSGETLLEKLQSAKEADLWRVLVALSIRHVGPTAAKALAKHFESVEDI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ASASAEEMAGIDGVAETIAESISDWFTVDWHRRIVDRWAAAGVRMRREAGNVPGAADGVD
HHCCHHHHHCCHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SALLEGLTIVVTGSLEDFDRTAAKEAIEARGGKAAGSVSKKTDFLVAGEKAGSKLKKAED
HHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHCHHHHHHHH
LGVPVLDEAAFKELLENGAPSSGDDASTSADSVDD
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA