Click here to switch to the map view.

The map label for this gene is menF [H]

Identifier: 68536367

GI number: 68536367

Start: 1513629

End: 1514771

Strand: Direct

Name: menF [H]

Synonym: jk1285

Alternate gene names: 68536367

Gene position: 1513629-1514771 (Clockwise)

Preceding gene: 68536362

Following gene: 68536369

Centisome position: 61.47

GC content: 64.74

Gene sequence:

>1143_bases
ATGACCCACGAGCAGCGACCGGCCACCGCCCCGGATTTCCTGCTTTCCCGCCCAGATCGCAGCATCCGCACCCAGGGGCG
CAAGGCGACCTTCCCAGACCCGTTCGAAGCCTCCACTGCGCTTCGAAACAAGGAAGCAGACTTGATTGTCGGCGCTTTCC
CCTTCGACACTTCTCTACGTGCTGCACTCATGGAGCCGGAGACTGTCGTCCGTGCCGAAGGCCCGCTGGAGCCACCCGCT
TTCTTCCGTGGCAAGGATGCCGCCCGCTCGTTGACGGTGGAGTCCGTTGAAGCGCAAACCACCGCCGAAGAGCACACCGC
GATGGTCGCCGCCGCCGTTGCCACGATCCAGCAGTCCCGCCTGGAGAAGGTCGTGCTTGCCCGCGCCGTGGACATCACCT
ACGCCGAAACCGTGGATCCACTGTTGATTGCCGCCCGCATGATCGACCTGTCCGCTTACCGCGACGGATTCGCCGTGGAC
CTCTCCGCCACGGGCCGCTCTGAGTACCAGGGGGCGATGTTCGTCGGCAGTTCGCCAGAGATGCTGATTCGTCGCGATGG
CCGCGAGATCTCGGCCTTCCCCCTGGCCGGTTCCGCGCCGCGCACCGGCAGCCTTTCTATTGATGACGCCACAGCTCGCG
AACTCGGAAGCTCCGACAAGGATCTACGCGAGCACCGCTTCGTGGTGGATCACTACCGCCGCGTGCTAGAGCCATTGTGC
GAGACGCTGGACATCCCCGCCACCCCGGAGATCCACGAGACCAACGAGATGATCCACCTGGGCACTCAGATCGCGGGCAC
GCTGAAAGATGACGAGTATTCCGCGCTGGATCTGGCCTTGATGCTGCACCCCACCCCGGCCATCGGCGGCACGCCGACTG
ACGATGCGCTGGGGGTTATTGCGCAGGAGAAGTCCCCGCGCGAGTTCTACTCCGGTGCCGTGGGCTGGTGCGATTCCAAC
GGCGACGGGGAGTTTATGCTCTCTATCCGCTGCGCCATCGTGGAGGACAACACCGCCCGCGCCTGGGCTGGCGGAGGCAT
TGTCGTGGATTCGATTCCCGCCATGGAGGCGGAGGAAACGTCCGCGAAGCTGCAAACAGCGCTGCGGGCACTCAACGTGC
CTGCAGCGCTGCGGGAAGTGTAG

Upstream 100 bases:

>100_bases
CCCTGCCCATCCCGAACCCGGGGCACACCACAGAACACAAGCCAGAGCACAAACCGGGGCGAGGCAGCCCGTTGTCTTGC
CGGGCAGTAAAATGGCACCC

Downstream 100 bases:

>100_bases
CGAGCGCCCGGGGCCAGCCATAGCCGGCCGGCGCGCTCCCGCCTACTTGCCGTAGGCCTGAACCGGGTTGGCCAGCTTAC
CCAGCCCCGGAATTTCCACC

Product: putative isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTHEQRPATAPDFLLSRPDRSIRTQGRKATFPDPFEASTALRNKEADLIVGAFPFDTSLRAALMEPETVVRAEGPLEPPA
FFRGKDAARSLTVESVEAQTTAEEHTAMVAAAVATIQQSRLEKVVLARAVDITYAETVDPLLIAARMIDLSAYRDGFAVD
LSATGRSEYQGAMFVGSSPEMLIRRDGREISAFPLAGSAPRTGSLSIDDATARELGSSDKDLREHRFVVDHYRRVLEPLC
ETLDIPATPEIHETNEMIHLGTQIAGTLKDDEYSALDLALMLHPTPAIGGTPTDDALGVIAQEKSPREFYSGAVGWCDSN
GDGEFMLSIRCAIVEDNTARAWAGGGIVVDSIPAMEAEETSAKLQTALRALNVPAALREV

Sequences:

>Translated_380_residues
MTHEQRPATAPDFLLSRPDRSIRTQGRKATFPDPFEASTALRNKEADLIVGAFPFDTSLRAALMEPETVVRAEGPLEPPA
FFRGKDAARSLTVESVEAQTTAEEHTAMVAAAVATIQQSRLEKVVLARAVDITYAETVDPLLIAARMIDLSAYRDGFAVD
LSATGRSEYQGAMFVGSSPEMLIRRDGREISAFPLAGSAPRTGSLSIDDATARELGSSDKDLREHRFVVDHYRRVLEPLC
ETLDIPATPEIHETNEMIHLGTQIAGTLKDDEYSALDLALMLHPTPAIGGTPTDDALGVIAQEKSPREFYSGAVGWCDSN
GDGEFMLSIRCAIVEDNTARAWAGGGIVVDSIPAMEAEETSAKLQTALRALNVPAALREV
>Mature_379_residues
THEQRPATAPDFLLSRPDRSIRTQGRKATFPDPFEASTALRNKEADLIVGAFPFDTSLRAALMEPETVVRAEGPLEPPAF
FRGKDAARSLTVESVEAQTTAEEHTAMVAAAVATIQQSRLEKVVLARAVDITYAETVDPLLIAARMIDLSAYRDGFAVDL
SATGRSEYQGAMFVGSSPEMLIRRDGREISAFPLAGSAPRTGSLSIDDATARELGSSDKDLREHRFVVDHYRRVLEPLCE
TLDIPATPEIHETNEMIHLGTQIAGTLKDDEYSALDLALMLHPTPAIGGTPTDDALGVIAQEKSPREFYSGAVGWCDSNG
DGEFMLSIRCAIVEDNTARAWAGGGIVVDSIPAMEAEETSAKLQTALRALNVPAALREV

Specific function: Might be involved in the biosynthesis of menaquinone. Converts chorismate to isochorismate [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=386, Percent_Identity=36.2694300518135, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87082077, Length=279, Percent_Identity=34.0501792114695, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787518, Length=209, Percent_Identity=32.5358851674641, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788114, Length=205, Percent_Identity=28.780487804878, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=198, Percent_Identity=32.8282828282828, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 41038; Mature: 40906

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHEQRPATAPDFLLSRPDRSIRTQGRKATFPDPFEASTALRNKEADLIVGAFPFDTSLR
CCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHH
AALMEPETVVRAEGPLEPPAFFRGKDAARSLTVESVEAQTTAEEHTAMVAAAVATIQQSR
HHHCCCCCEEECCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKVVLARAVDITYAETVDPLLIAARMIDLSAYRDGFAVDLSATGRSEYQGAMFVGSSPE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCC
MLIRRDGREISAFPLAGSAPRTGSLSIDDATARELGSSDKDLREHRFVVDHYRRVLEPLC
EEEECCCCEEEEECCCCCCCCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ETLDIPATPEIHETNEMIHLGTQIAGTLKDDEYSALDLALMLHPTPAIGGTPTDDALGVI
HHCCCCCCCCCCCCCCEEEECHHHEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHEEE
AQEKSPREFYSGAVGWCDSNGDGEFMLSIRCAIVEDNTARAWAGGGIVVDSIPAMEAEET
ECCCCCHHHHCCCCEEECCCCCCEEEEEEEEEEEECCCCCEEECCCEEEECCCCCCCHHH
SAKLQTALRALNVPAALREV
HHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
THEQRPATAPDFLLSRPDRSIRTQGRKATFPDPFEASTALRNKEADLIVGAFPFDTSLR
CCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHH
AALMEPETVVRAEGPLEPPAFFRGKDAARSLTVESVEAQTTAEEHTAMVAAAVATIQQSR
HHHCCCCCEEECCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKVVLARAVDITYAETVDPLLIAARMIDLSAYRDGFAVDLSATGRSEYQGAMFVGSSPE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCC
MLIRRDGREISAFPLAGSAPRTGSLSIDDATARELGSSDKDLREHRFVVDHYRRVLEPLC
EEEECCCCEEEEECCCCCCCCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ETLDIPATPEIHETNEMIHLGTQIAGTLKDDEYSALDLALMLHPTPAIGGTPTDDALGVI
HHCCCCCCCCCCCCCCEEEECHHHEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHEEE
AQEKSPREFYSGAVGWCDSNGDGEFMLSIRCAIVEDNTARAWAGGGIVVDSIPAMEAEET
ECCCCCHHHHCCCCEEECCCCCCEEEEEEEEEEEECCCCCEEECCCEEEECCCCCCCHHH
SAKLQTALRALNVPAALREV
HHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]