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The map label for this gene is sigB [H]

Identifier: 68536171

GI number: 68536171

Start: 1286462

End: 1287493

Strand: Direct

Name: sigB [H]

Synonym: jk1094

Alternate gene names: 68536171

Gene position: 1286462-1287493 (Clockwise)

Preceding gene: 68536170

Following gene: 68536174

Centisome position: 52.24

GC content: 59.11

Gene sequence:

>1032_bases
ATGAAAAACTCCATGATTGATGACGAATACAACGGCATCAACAACGGTCAGGGCAACGGCGATTCGACCGACGATAGCGA
CAAAAAGGAAGACAAGGGGCGGCGCCGAGGCAACAACGAGAACCCGTCGGCAGATTTGGTACGCGTATACCTCAACGGCA
TCGGCAAGACAGCCTTGCTGACGGCCGAGGACGAGGTCGAACTATCCAAGCGCATCGAGGTTGGCCTGTACTCGGAGTAC
CTGCTGTCCAACGCTGAGAAGCTGACCCGCGCAAAGAAACGTGACCTGAAGGTTTTAGCTCGCGAAGGCAAGGCAGCCCG
CAGCCACCTGCTGGAAGCGAACCTGCGACTGGTGGTATCCCTGGCTAAGCGCTACACCGGTCGCGGAATGCCACTGCTGG
ACCTGATCCAGGAGGGCAACCTGGGCCTTATCCGCGCCATGGAGAAATTCGACTACACCAAGGGTTTTAAATTCTCCACC
TACGCAACGTGGTGGATCCGCCAGGCCATCACCCGTGGCATGGCCGACCAGTCCCGAACCATCCGCCTTCCCGTTCACCT
CGTTGAGCAAGTCAACAAGCTGTCCCGGATCAAGCGCGAAATGTATCAGCAGCTGGGCCGTGAAGCCACCAATGAGGAGC
TATCGGAGGAGTCCGGCATCGAAGAGTCCAAGATCGAGATGCTGCTGAAGCAGTCTCGCGATCCGGTGAGCCTGGACATG
CCCGTGGGCAGCGATGAGGAAGCCCCGCTGGGCGATTTCATTGAAGATTCCGATGCCACAGACGCGGAGACTGCCGTTGT
AGCTTCCCTGCGCCACTCCGATGTACGCGCGGTTCTGGGCACACTGGAAGAGCGCGAGCAAGACGTCATCAAGCTGCGCT
ACGGCCTAGACGATGGCATGCCCCGTACCCTCGATCAGATCGGCCGCCGCTTCGGGCTTTCCCGCGAGCGTGTGCGACAG
ATTGAGCGCGAGGTTATGGCAAAGCTGCGCGAAGGCGATCGAGCAGACAAGCTGCGCGAATACGCGGTCTAA

Upstream 100 bases:

>100_bases
CAACGAAGATGTCCGTTAACCCCTTAGAGCTTCGGCACATAACGAGAAAAATCTTGAACGAGCTTGAGCGCTATCGAGCC
ACGGGAGGCACACCAGTACC

Downstream 100 bases:

>100_bases
CCACCCCGCCGGAAAACAAGTACCTATACTTATGCCCCGTTTCCTAAGTGAACGGGGGGTTAAAGGACAAAATGTGTGTC
TTTCCCGGGTGTGTCGTGGT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKNSMIDDEYNGINNGQGNGDSTDDSDKKEDKGRRRGNNENPSADLVRVYLNGIGKTALLTAEDEVELSKRIEVGLYSEY
LLSNAEKLTRAKKRDLKVLAREGKAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFST
YATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLSRIKREMYQQLGREATNEELSEESGIEESKIEMLLKQSRDPVSLDM
PVGSDEEAPLGDFIEDSDATDAETAVVASLRHSDVRAVLGTLEEREQDVIKLRYGLDDGMPRTLDQIGRRFGLSRERVRQ
IEREVMAKLREGDRADKLREYAV

Sequences:

>Translated_343_residues
MKNSMIDDEYNGINNGQGNGDSTDDSDKKEDKGRRRGNNENPSADLVRVYLNGIGKTALLTAEDEVELSKRIEVGLYSEY
LLSNAEKLTRAKKRDLKVLAREGKAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFST
YATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLSRIKREMYQQLGREATNEELSEESGIEESKIEMLLKQSRDPVSLDM
PVGSDEEAPLGDFIEDSDATDAETAVVASLRHSDVRAVLGTLEEREQDVIKLRYGLDDGMPRTLDQIGRRFGLSRERVRQ
IEREVMAKLREGDRADKLREYAV
>Mature_343_residues
MKNSMIDDEYNGINNGQGNGDSTDDSDKKEDKGRRRGNNENPSADLVRVYLNGIGKTALLTAEDEVELSKRIEVGLYSEY
LLSNAEKLTRAKKRDLKVLAREGKAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFST
YATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLSRIKREMYQQLGREATNEELSEESGIEESKIEMLLKQSRDPVSLDM
PVGSDEEAPLGDFIEDSDATDAETAVVASLRHSDVRAVLGTLEEREQDVIKLRYGLDDGMPRTLDQIGRRFGLSRERVRQ
IEREVMAKLREGDRADKLREYAV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=51.0288065843621, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=39.7212543554007, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=30.2521008403361, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38910; Mature: 38910

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSMIDDEYNGINNGQGNGDSTDDSDKKEDKGRRRGNNENPSADLVRVYLNGIGKTALL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
TAEDEVELSKRIEVGLYSEYLLSNAEKLTRAKKRDLKVLAREGKAARSHLLEANLRLVVS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRT
HHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCE
IRLPVHLVEQVNKLSRIKREMYQQLGREATNEELSEESGIEESKIEMLLKQSRDPVSLDM
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEC
PVGSDEEAPLGDFIEDSDATDAETAVVASLRHSDVRAVLGTLEEREQDVIKLRYGLDDGM
CCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRTLDQIGRRFGLSRERVRQIEREVMAKLREGDRADKLREYAV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MKNSMIDDEYNGINNGQGNGDSTDDSDKKEDKGRRRGNNENPSADLVRVYLNGIGKTALL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
TAEDEVELSKRIEVGLYSEYLLSNAEKLTRAKKRDLKVLAREGKAARSHLLEANLRLVVS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRT
HHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCE
IRLPVHLVEQVNKLSRIKREMYQQLGREATNEELSEESGIEESKIEMLLKQSRDPVSLDM
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEC
PVGSDEEAPLGDFIEDSDATDAETAVVASLRHSDVRAVLGTLEEREQDVIKLRYGLDDGM
CCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRTLDQIGRRFGLSRERVRQIEREVMAKLREGDRADKLREYAV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]