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The map label for this gene is tnp3514a [H]

Identifier: 68535601

GI number: 68535601

Start: 641672

End: 642859

Strand: Direct

Name: tnp3514a [H]

Synonym: jk0529

Alternate gene names: 68535601

Gene position: 641672-642859 (Clockwise)

Preceding gene: 68535598

Following gene: 68535602

Centisome position: 26.06

GC content: 55.64

Gene sequence:

>1188_bases
ATGAATAGTCCCAACCGCAACCTCGCCATCGTCAAGGCAGTCCGCGAACAAGGCGAACCCGTCACCAAAGTCGCCAAACG
CTTCAGAATCTCACGGCAACGTATCTACAAAATCCTGTCCCAGTTCGATGCCGGAGGCGCTGATGCCATCGCCCCGAAAT
CCCGCGCCCCGCATACCCACCCGCAAGCAGTACCAACATCTTTACGCAACCAGATCATCGACATGCGTAAGCAACTGGTC
AGATCCGGTTTAGACGCAGGCCCTGAGACCATCGCATTCCACCTTCACCGCCAGGGGTTGCGAGTACCGTCAACGTCGAC
CATCCGACGGATCATCACCAACGCAGGCCTTGTCACGCCACAACCGCAGAAAAAGCCACGCAGTTCTTTCATCCGTTTCG
AAGCCGCTATGCCCAACGAATGCTGGCAGGCAGATATCACCCACCTACACTTGCTCGACGGCACTCGACTCGAAGTCCTC
GACTTCATCGACGATCACTCCCGTTACCTGCTATCGATCACCGCAGCAGCATCCTTCAGCGGACCGGCTGTTGCAGCTGA
ACTGCAACGACTCATCGCCACCTACGGCCCGCCGGCATCCACCCTCACCGACAACGGACTCGTATTCACCGCCCGCCTAG
CTGGAGCCAGAGGGGGACGCAACGCCTTCGAAAAAACCCTCAACAAATACCGCATCCAACAGAAAAATGGCCGGCCAGGA
CATCCCCAAACACAAGGGAAGATCGAACGGTTCCACCAAACCCTTAAAAAATGGATTGCTGCCCAATCCCCAGCGATCAC
CCTGGTCGAACTCCAACGGCAACTCGACACCTTTGCCGACTACTACAACACTGTCCGACCTCACCGAGCCCTCGGAAGAC
GGACACCACATGAGGTCTACACCACCGGCCCCAAAGCCGAACCCAACGACAAACCGGAAGAAGAATGGCGCGTCCGCAAT
GATGTCGTCACCCCAAACGGCAAGGTAACCGTCCGTTACGCAAGTAGGCTCTACCAACTTGGCATCGGGCGAAAATACAC
TGGCGAAACCATCCTTATGGTCATCACCGACAACCATGTCACCACATCATTAAAAGAAACTGGCGAGATCATCACCGAGC
ACTACATCGACACGTCTCGCAATTACCAAAAACCTTATTGGAAGAAAGGCCAACCACCCCTGACCTAA

Upstream 100 bases:

>100_bases
GTTGTGACGGTTGTCAGATCAGCCGCGACATAGTTGACAGTTAGCCACCACAAAGCAAAAGCGGTGATGGTTGACATCTC
AGCTGCGACATTGTTGACAG

Downstream 100 bases:

>100_bases
AAGCGAAACCGGCGCCTCGGAAAAAGAAAAACCGAGACGCCAGTTTGTCCATGATGTCGCGACTGAAGTGTCAACTATGT
CGCGACTCATGACAATGGCG

Product: transposase for IS3514a

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNSPNRNLAIVKAVREQGEPVTKVAKRFRISRQRIYKILSQFDAGGADAIAPKSRAPHTHPQAVPTSLRNQIIDMRKQLV
RSGLDAGPETIAFHLHRQGLRVPSTSTIRRIITNAGLVTPQPQKKPRSSFIRFEAAMPNECWQADITHLHLLDGTRLEVL
DFIDDHSRYLLSITAAASFSGPAVAAELQRLIATYGPPASTLTDNGLVFTARLAGARGGRNAFEKTLNKYRIQQKNGRPG
HPQTQGKIERFHQTLKKWIAAQSPAITLVELQRQLDTFADYYNTVRPHRALGRRTPHEVYTTGPKAEPNDKPEEEWRVRN
DVVTPNGKVTVRYASRLYQLGIGRKYTGETILMVITDNHVTTSLKETGEIITEHYIDTSRNYQKPYWKKGQPPLT

Sequences:

>Translated_395_residues
MNSPNRNLAIVKAVREQGEPVTKVAKRFRISRQRIYKILSQFDAGGADAIAPKSRAPHTHPQAVPTSLRNQIIDMRKQLV
RSGLDAGPETIAFHLHRQGLRVPSTSTIRRIITNAGLVTPQPQKKPRSSFIRFEAAMPNECWQADITHLHLLDGTRLEVL
DFIDDHSRYLLSITAAASFSGPAVAAELQRLIATYGPPASTLTDNGLVFTARLAGARGGRNAFEKTLNKYRIQQKNGRPG
HPQTQGKIERFHQTLKKWIAAQSPAITLVELQRQLDTFADYYNTVRPHRALGRRTPHEVYTTGPKAEPNDKPEEEWRVRN
DVVTPNGKVTVRYASRLYQLGIGRKYTGETILMVITDNHVTTSLKETGEIITEHYIDTSRNYQKPYWKKGQPPLT
>Mature_395_residues
MNSPNRNLAIVKAVREQGEPVTKVAKRFRISRQRIYKILSQFDAGGADAIAPKSRAPHTHPQAVPTSLRNQIIDMRKQLV
RSGLDAGPETIAFHLHRQGLRVPSTSTIRRIITNAGLVTPQPQKKPRSSFIRFEAAMPNECWQADITHLHLLDGTRLEVL
DFIDDHSRYLLSITAAASFSGPAVAAELQRLIATYGPPASTLTDNGLVFTARLAGARGGRNAFEKTLNKYRIQQKNGRPG
HPQTQGKIERFHQTLKKWIAAQSPAITLVELQRQLDTFADYYNTVRPHRALGRRTPHEVYTTGPKAEPNDKPEEEWRVRN
DVVTPNGKVTVRYASRLYQLGIGRKYTGETILMVITDNHVTTSLKETGEIITEHYIDTSRNYQKPYWKKGQPPLT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=354, Percent_Identity=29.6610169491525, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 44486; Mature: 44486

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPNRNLAIVKAVREQGEPVTKVAKRFRISRQRIYKILSQFDAGGADAIAPKSRAPHTH
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PQAVPTSLRNQIIDMRKQLVRSGLDAGPETIAFHLHRQGLRVPSTSTIRRIITNAGLVTP
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHCCCCCCCHHHHHHHHHHCCCCCC
QPQKKPRSSFIRFEAAMPNECWQADITHLHLLDGTRLEVLDFIDDHSRYLLSITAAASFS
CCCCCCHHHHEEEECCCCCHHHHHCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCC
GPAVAAELQRLIATYGPPASTLTDNGLVFTARLAGARGGRNAFEKTLNKYRIQQKNGRPG
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
HPQTQGKIERFHQTLKKWIAAQSPAITLVELQRQLDTFADYYNTVRPHRALGRRTPHEVY
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHE
TTGPKAEPNDKPEEEWRVRNDVVTPNGKVTVRYASRLYQLGIGRKYTGETILMVITDNHV
ECCCCCCCCCCCHHHHHHHCCEECCCCCEEEHHHHHHHHHCCCCEECCCEEEEEEECCCH
TTSLKETGEIITEHYIDTSRNYQKPYWKKGQPPLT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSPNRNLAIVKAVREQGEPVTKVAKRFRISRQRIYKILSQFDAGGADAIAPKSRAPHTH
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PQAVPTSLRNQIIDMRKQLVRSGLDAGPETIAFHLHRQGLRVPSTSTIRRIITNAGLVTP
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHCCCCCCCHHHHHHHHHHCCCCCC
QPQKKPRSSFIRFEAAMPNECWQADITHLHLLDGTRLEVLDFIDDHSRYLLSITAAASFS
CCCCCCHHHHEEEECCCCCHHHHHCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCC
GPAVAAELQRLIATYGPPASTLTDNGLVFTARLAGARGGRNAFEKTLNKYRIQQKNGRPG
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
HPQTQGKIERFHQTLKKWIAAQSPAITLVELQRQLDTFADYYNTVRPHRALGRRTPHEVY
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHE
TTGPKAEPNDKPEEEWRVRNDVVTPNGKVTVRYASRLYQLGIGRKYTGETILMVITDNHV
ECCCCCCCCCCCHHHHHHHCCEECCCCCEEEHHHHHHHHHCCCCEECCCEEEEEEECCCH
TTSLKETGEIITEHYIDTSRNYQKPYWKKGQPPLT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8113168 [H]