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The map label for this gene is nrdE [H]

Identifier: 68535548

GI number: 68535548

Start: 581056

End: 583218

Strand: Direct

Name: nrdE [H]

Synonym: jk0478

Alternate gene names: 68535548

Gene position: 581056-583218 (Clockwise)

Preceding gene: 68535547

Following gene: 68535550

Centisome position: 23.6

GC content: 58.76

Gene sequence:

>2163_bases
ATGACTGGCACCGAGCTTGGCAAGACCGTCCAGGAGCCCGTTGACCAGCGGGAGCAGCTGGATTATCACGCTCTGAACGC
GATGTTGAACCTGTACAACGATGCAGGGGAGATCCAGTTCGACAAGGATCGCGAGGCTGCAAACCAGTACTTCCTGCAGC
ATGTCAACCAGAACACCGTTTACTTCCACGATCTGGAAGAAAAGGTTAAGTACCTGGTGGACAACGAGTACTACGACCCC
GAGGTGCTGGATCAGTACGAGTTCAGCTTTGTGAAGTCCCTGTTTAAGCAGGCCTACGCGAAGAAGTTCCGCTTCCAGTC
GTTCCTGGGTGCGTACAAGTACTACACCTCCTACACACTGAAGACCTTCGACGGTCGCCGTTACCTGGAGCGTTTCGAGG
ACCGCGTGTGCATGGTTGCATTGGGGCTGGCGGCGGGGGATACCGAGTTGGCGTCCAACCTGGTCGACGAGATCATGGAC
GGCCGCTTCCAGCCTGCAACGCCAACCTTCCTCAACATCGGCAAGAAGCAGCGCGGTGAGCCGGTTTCGTGCTTCCTGCT
GCGCATCGAGGACAACATGGAGTCCATCGGCCGTTCCATCAACTCCGCGCTGCAGCTGTCCAAGCGCGGCGGCGGTGTGG
CTCTGCTGCTGTCGAACGTGCGTGAAGCCGGCGCGCCGATTAAGCACATCGAGAACCAGTCCTCCGGCGTGATCCCCGTG
ATGAAGCTGCTGGAAGATGCCTTCAGCTACGCCAACCAGCTGGGCGCCCGCCAGGGTGCCGGTGCGGTCTACCTGAGCGC
GCACCACCCGGACATCCTGCGCTTCCTGGATACCAAGCGTGAGAACGCGGACGAAAAGATTCGCATTAAGACCCTCTCGC
TGGGCGTGGTGATCCCGGACATTACCTTCGAGCTAGCCAAGCGCAACGACGACATGTACCTGTTCAGCCCGTACGACGTC
GAGCGCGTGTACGGCAAGCCGTTCGCCGACGTCTCCATCACCGAACACTACGAGGAGATGGTGGAGGATCCGCGAATCCG
CAAGTCCAAGATCAACGCGCGTGCGTTCTTCCAGACCCTGGCGGAGATCCAGTTCGAGTCCGGATACCCGTACATCATGT
TCGAGGACACGGTTAATAAGGCCAACCCGATCGAGGGCCGCATTACCCACTCGAACCTATGCTCCGAGATTCTGCAGGTC
TCCACCCCGTCGGAGTTCAATGCTGACCTGTCGTACAAGCAGATCGGCGACGATATTTCTTGCAACCTGGGCTCGATGAA
TATTGCGAAGGCGATGGATTCGGAGGACTTCTCCAAGACCATCGAGACCGCCATCCGTGGCCTGACCGCCGTGGCGGACC
AGACCTCCATTGACTCCGTGCCTTCGGTGCGCCAGGGTAACGACAACTCCCACGCCATCGGCCTGGGCCAGATGAACCTG
CACGGATTCCTGGGGCGTGAGCACATCCACTACGGTTCCGAAGAGGCGCTGGACTTCACCAACGCCTACTTTGCGACCGT
GATGTATGAGGCGTTGAAGGCTTCCAACAAGATCGCCCAGGAGCGCGGGGAGAAGTTCCACAACTTCGAAAACTCCCAGT
ACGCCACCGGAGAGTTCTTCGACCGCTACGATCCGGCCGAGTTCGCCCCCAAGACGGAGCGTATCCGCCAAATCATCGGC
AACTCCAGCATCCAGGTGCCAACCGCGCAGGACTGGCAACAGCTGAAGGACGATGTGCAGGCTCACGGTCTATACAACCG
CAACCTGCAGGCTATTCCGCCGACGGGTTCCATCTCCTACATCAACAACTCCACGTCCTCGATTCACCCGATCGCCTCCA
AGATCGAAATTCGTAAGGAGGGCAAGATCGGTCGTGTGTACTACCCGGCTCCGTACATGACGAACGACAACCTGGAGTAC
TACGAGGACGCGTACGAGATCGGCTACGAAAAGATCATCGACACTTACGCAGTCGCGACGAAGTACGTGGATCAGGGCCT
ATCGCTGACCCTGTTCTTCAAGGACACCGCCACCACGCGCGACATCAACCGTGCGCAGATCTATGCGTGGCGCAAGGGGA
TCAAGACGATCTACTACATCCGCCTCCGCCAGACGGCCTTGACCGGCACCGAGGTTGAGGGCTGCGTGAGCTGCATGCTC
TAA

Upstream 100 bases:

>100_bases
ACCCGCCCCGCACATGTCCGGGGCACTGATTAGAATCGAAACGAACGCACGAGCGCGAGACATTTTTCTTAGCGCGAGTT
TTTGAGGAGAACACATACAC

Downstream 100 bases:

>100_bases
GTGGGGAGGGGAGCGGCCTGCTCTAGGTGGAGGGCGGCCTCCACCGAGCATGAAAAAGCCGACTGGGAGGAATCCCAGTC
GGCTTTTGTTATTGGCCGTT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTGTELGKTVQEPVDQREQLDYHALNAMLNLYNDAGEIQFDKDREAANQYFLQHVNQNTVYFHDLEEKVKYLVDNEYYDP
EVLDQYEFSFVKSLFKQAYAKKFRFQSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALGLAAGDTELASNLVDEIMD
GRFQPATPTFLNIGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNVREAGAPIKHIENQSSGVIPV
MKLLEDAFSYANQLGARQGAGAVYLSAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDV
ERVYGKPFADVSITEHYEEMVEDPRIRKSKINARAFFQTLAEIQFESGYPYIMFEDTVNKANPIEGRITHSNLCSEILQV
STPSEFNADLSYKQIGDDISCNLGSMNIAKAMDSEDFSKTIETAIRGLTAVADQTSIDSVPSVRQGNDNSHAIGLGQMNL
HGFLGREHIHYGSEEALDFTNAYFATVMYEALKASNKIAQERGEKFHNFENSQYATGEFFDRYDPAEFAPKTERIRQIIG
NSSIQVPTAQDWQQLKDDVQAHGLYNRNLQAIPPTGSISYINNSTSSIHPIASKIEIRKEGKIGRVYYPAPYMTNDNLEY
YEDAYEIGYEKIIDTYAVATKYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTIYYIRLRQTALTGTEVEGCVSCML

Sequences:

>Translated_720_residues
MTGTELGKTVQEPVDQREQLDYHALNAMLNLYNDAGEIQFDKDREAANQYFLQHVNQNTVYFHDLEEKVKYLVDNEYYDP
EVLDQYEFSFVKSLFKQAYAKKFRFQSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALGLAAGDTELASNLVDEIMD
GRFQPATPTFLNIGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNVREAGAPIKHIENQSSGVIPV
MKLLEDAFSYANQLGARQGAGAVYLSAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDV
ERVYGKPFADVSITEHYEEMVEDPRIRKSKINARAFFQTLAEIQFESGYPYIMFEDTVNKANPIEGRITHSNLCSEILQV
STPSEFNADLSYKQIGDDISCNLGSMNIAKAMDSEDFSKTIETAIRGLTAVADQTSIDSVPSVRQGNDNSHAIGLGQMNL
HGFLGREHIHYGSEEALDFTNAYFATVMYEALKASNKIAQERGEKFHNFENSQYATGEFFDRYDPAEFAPKTERIRQIIG
NSSIQVPTAQDWQQLKDDVQAHGLYNRNLQAIPPTGSISYINNSTSSIHPIASKIEIRKEGKIGRVYYPAPYMTNDNLEY
YEDAYEIGYEKIIDTYAVATKYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTIYYIRLRQTALTGTEVEGCVSCML
>Mature_719_residues
TGTELGKTVQEPVDQREQLDYHALNAMLNLYNDAGEIQFDKDREAANQYFLQHVNQNTVYFHDLEEKVKYLVDNEYYDPE
VLDQYEFSFVKSLFKQAYAKKFRFQSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALGLAAGDTELASNLVDEIMDG
RFQPATPTFLNIGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNVREAGAPIKHIENQSSGVIPVM
KLLEDAFSYANQLGARQGAGAVYLSAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVE
RVYGKPFADVSITEHYEEMVEDPRIRKSKINARAFFQTLAEIQFESGYPYIMFEDTVNKANPIEGRITHSNLCSEILQVS
TPSEFNADLSYKQIGDDISCNLGSMNIAKAMDSEDFSKTIETAIRGLTAVADQTSIDSVPSVRQGNDNSHAIGLGQMNLH
GFLGREHIHYGSEEALDFTNAYFATVMYEALKASNKIAQERGEKFHNFENSQYATGEFFDRYDPAEFAPKTERIRQIIGN
SSIQVPTAQDWQQLKDDVQAHGLYNRNLQAIPPTGSISYINNSTSSIHPIASKIEIRKEGKIGRVYYPAPYMTNDNLEYY
EDAYEIGYEKIIDTYAVATKYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTIYYIRLRQTALTGTEVEGCVSCML

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=26.0670731707317, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1789030, Length=715, Percent_Identity=70.4895104895105, Blast_Score=1066, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=540, Percent_Identity=25.9259259259259, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17555242, Length=644, Percent_Identity=26.8633540372671, Blast_Score=195, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6322123, Length=645, Percent_Identity=27.1317829457364, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=27.217125382263, Blast_Score=197, Evalue=5e-51,
Organism=Drosophila melanogaster, GI17352481, Length=665, Percent_Identity=25.7142857142857, Blast_Score=193, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81751; Mature: 81620

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTELGKTVQEPVDQREQLDYHALNAMLNLYNDAGEIQFDKDREAANQYFLQHVNQNTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEE
YFHDLEEKVKYLVDNEYYDPEVLDQYEFSFVKSLFKQAYAKKFRFQSFLGAYKYYTSYTL
EEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
KTFDGRRYLERFEDRVCMVALGLAAGDTELASNLVDEIMDGRFQPATPTFLNIGKKQRGE
EECCCHHHHHHHHCCEEEEHEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCC
PVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNVREAGAPIKHIENQSSGVIPV
CEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCHHHHCCCCCCCHHH
MKLLEDAFSYANQLGARQGAGAVYLSAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPD
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECC
ITFELAKRNDDMYLFSPYDVERVYGKPFADVSITEHYEEMVEDPRIRKSKINARAFFQTL
HHHHHHHCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AEIQFESGYPYIMFEDTVNKANPIEGRITHSNLCSEILQVSTPSEFNADLSYKQIGDDIS
HHHHCCCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCC
CNLGSMNIAKAMDSEDFSKTIETAIRGLTAVADQTSIDSVPSVRQGNDNSHAIGLGQMNL
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCH
HGFLGREHIHYGSEEALDFTNAYFATVMYEALKASNKIAQERGEKFHNFENSQYATGEFF
HHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
DRYDPAEFAPKTERIRQIIGNSSIQVPTAQDWQQLKDDVQAHGLYNRNLQAIPPTGSISY
HCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCEEE
INNSTSSIHPIASKIEIRKEGKIGRVYYPAPYMTNDNLEYYEDAYEIGYEKIIDTYAVAT
ECCCCCCCCHHHHHHEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTIYYIRLRQTALTGTEVEGCVSCML
HHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
TGTELGKTVQEPVDQREQLDYHALNAMLNLYNDAGEIQFDKDREAANQYFLQHVNQNTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEE
YFHDLEEKVKYLVDNEYYDPEVLDQYEFSFVKSLFKQAYAKKFRFQSFLGAYKYYTSYTL
EEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
KTFDGRRYLERFEDRVCMVALGLAAGDTELASNLVDEIMDGRFQPATPTFLNIGKKQRGE
EECCCHHHHHHHHCCEEEEHEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCC
PVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNVREAGAPIKHIENQSSGVIPV
CEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCHHHHCCCCCCCHHH
MKLLEDAFSYANQLGARQGAGAVYLSAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPD
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECC
ITFELAKRNDDMYLFSPYDVERVYGKPFADVSITEHYEEMVEDPRIRKSKINARAFFQTL
HHHHHHHCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AEIQFESGYPYIMFEDTVNKANPIEGRITHSNLCSEILQVSTPSEFNADLSYKQIGDDIS
HHHHCCCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCC
CNLGSMNIAKAMDSEDFSKTIETAIRGLTAVADQTSIDSVPSVRQGNDNSHAIGLGQMNL
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCH
HGFLGREHIHYGSEEALDFTNAYFATVMYEALKASNKIAQERGEKFHNFENSQYATGEFF
HHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
DRYDPAEFAPKTERIRQIIGNSSIQVPTAQDWQQLKDDVQAHGLYNRNLQAIPPTGSISY
HCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCEEE
INNSTSSIHPIASKIEIRKEGKIGRVYYPAPYMTNDNLEYYEDAYEIGYEKIIDTYAVAT
ECCCCCCCCHHHHHHEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTIYYIRLRQTALTGTEVEGCVSCML
HHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]