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The map label for this gene is prfB [H]

Identifier: 68535523

GI number: 68535523

Start: 546429

End: 547562

Strand: Direct

Name: prfB [H]

Synonym: jk0453

Alternate gene names: 68535523

Gene position: 546429-547562 (Clockwise)

Preceding gene: 68535520

Following gene: 68535526

Centisome position: 22.19

GC content: 62.79

Gene sequence:

>1134_bases
ATGCAACCGGAAGTTTCTGCAGACCTAAGCGACCTGGACGGCACCCTAACCACGATCGAGAAGGTGCTGGATCTCGACGA
GCTGGGCGACCGCGCCCGCGAACTGGAGGAAAAGTCCGCCGACCCGTCCCTGTGGGACGACCCGGACCACGCGCAGCAGG
TCACTTCCGAACTCTCCCGAGTGCAGTCAAAGTTGAAGAAAGTCAAGGGGTTACGCCAACGCCTGGATGACCTTCCGGTG
CTCTACGACATGGCTGAGGAGGAAGCCGGGGACGATCCGGACGCGGCTCAGGAAGCGGTGCAGCTGGCCGACACGGAACG
GGAGGAGCTGCGCCAGGAGATCGAATCGCTCGAGGTCACGACGATGCTGTCGGGGGAGTACGACGAGCGTGAGGCAGTGC
TGAACATCCGCAGTGCGGCCGGCGGCGTGGATGCCGCCGACTGGGCAGAAATGCTGCTGCGCATGTACACCCGCTGGGCC
GAAAAGGCCGGGCACAAGGTGGAGGTCTACGACATCTCCTACGCCGAGGAAGCCGGCATTAAGTCCGCGACCATCGTCAT
CCACGGCGAATACATGTACGGCACCCTCTCCGTGGAGCAGGGAACCCACCGCCTGGTGCGCATTTCCCCCTTCGACAACC
AGGGCCGCCGCCAGACTTCGTTTGCCGAGGTGGAGGTGCTGCCAGTGGTGGAGCAGACCGACCACATCGACATTGACGAA
AACGACGTACGAGTGGACGTCTACCGCTCCTCTGGCCCCGGCGGGCAGTCGGTGAACACCACCGACTCGGCGGTGCGCCT
AACCCACGTGCCCACCGGCATTGTGGTGACATGCCAGAACGAAAAATCGCAGATTCAGAACAAGGCATCCGCTATGCGGG
TGCTGCAGGCGAAGCTGCTGGAGCGTAAGCGTCAAGAAGAGCAGGCCGAAATGGATGCGCTGAAGGGCGACGGCTCGAAC
TCCTGGGGTAACCAGATGCGCAACTATGTGCTGCACCCGTACCAGATGGTGAAGGACCTACGCACGAACGTCGAGGTCAA
CGACCCCAGTAAGGTGCTGGATGGTGACCTCGACGGCTTCCTGGAGGCCGGCATCCGCTGGCGCATGGCGCAGCAGCAGG
AAGCACAGGCCTAG

Upstream 100 bases:

>100_bases
TGATTGCGTTCAGCATGTCCTCTGGGATCGGGGAAGAAGAATCAGAACCGTTAGTAGCCATACATTCCATTGTGCATGTC
GCCGGGTAGACTTGCGCATT

Downstream 100 bases:

>100_bases
CGCGCCGGGCTGTACGCGAGCGCGCGCCGGGCTGCGTGCCTGGCGGTGGCTGAGCCAGGGCTGCGTGCCCGGTCAGGCTC
AGCGCTGCGCCTTAAGGGGT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MQPEVSADLSDLDGTLTTIEKVLDLDELGDRARELEEKSADPSLWDDPDHAQQVTSELSRVQSKLKKVKGLRQRLDDLPV
LYDMAEEEAGDDPDAAQEAVQLADTEREELRQEIESLEVTTMLSGEYDEREAVLNIRSAAGGVDAADWAEMLLRMYTRWA
EKAGHKVEVYDISYAEEAGIKSATIVIHGEYMYGTLSVEQGTHRLVRISPFDNQGRRQTSFAEVEVLPVVEQTDHIDIDE
NDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEEQAEMDALKGDGSN
SWGNQMRNYVLHPYQMVKDLRTNVEVNDPSKVLDGDLDGFLEAGIRWRMAQQQEAQA

Sequences:

>Translated_377_residues
MQPEVSADLSDLDGTLTTIEKVLDLDELGDRARELEEKSADPSLWDDPDHAQQVTSELSRVQSKLKKVKGLRQRLDDLPV
LYDMAEEEAGDDPDAAQEAVQLADTEREELRQEIESLEVTTMLSGEYDEREAVLNIRSAAGGVDAADWAEMLLRMYTRWA
EKAGHKVEVYDISYAEEAGIKSATIVIHGEYMYGTLSVEQGTHRLVRISPFDNQGRRQTSFAEVEVLPVVEQTDHIDIDE
NDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEEQAEMDALKGDGSN
SWGNQMRNYVLHPYQMVKDLRTNVEVNDPSKVLDGDLDGFLEAGIRWRMAQQQEAQA
>Mature_377_residues
MQPEVSADLSDLDGTLTTIEKVLDLDELGDRARELEEKSADPSLWDDPDHAQQVTSELSRVQSKLKKVKGLRQRLDDLPV
LYDMAEEEAGDDPDAAQEAVQLADTEREELRQEIESLEVTTMLSGEYDEREAVLNIRSAAGGVDAADWAEMLLRMYTRWA
EKAGHKVEVYDISYAEEAGIKSATIVIHGEYMYGTLSVEQGTHRLVRISPFDNQGRRQTSFAEVEVLPVVEQTDHIDIDE
NDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEEQAEMDALKGDGSN
SWGNQMRNYVLHPYQMVKDLRTNVEVNDPSKVLDGDLDGFLEAGIRWRMAQQQEAQA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=32.9931972789116, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI34577120, Length=269, Percent_Identity=33.457249070632, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI166795305, Length=177, Percent_Identity=33.8983050847458, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI2367172, Length=363, Percent_Identity=44.3526170798898, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1787462, Length=249, Percent_Identity=41.3654618473896, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=218, Percent_Identity=32.1100917431193, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=32.5732899022801, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19921226, Length=232, Percent_Identity=32.7586206896552, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42262; Mature: 42262

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPEVSADLSDLDGTLTTIEKVLDLDELGDRARELEEKSADPSLWDDPDHAQQVTSELSR
CCCCCCCCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VQSKLKKVKGLRQRLDDLPVLYDMAEEEAGDDPDAAQEAVQLADTEREELRQEIESLEVT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TMLSGEYDEREAVLNIRSAAGGVDAADWAEMLLRMYTRWAEKAGHKVEVYDISYAEEAGI
HEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCC
KSATIVIHGEYMYGTLSVEQGTHRLVRISPFDNQGRRQTSFAEVEVLPVVEQTDHIDIDE
CEEEEEEEECEEEEEEEECCCCCEEEEECCCCCCCCCCCCHHEEEEEEEECCCCCCCCCC
NDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGIVVTCQNEKSQIQNKASAMRVLQAKLL
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ERKRQEEQAEMDALKGDGSNSWGNQMRNYVLHPYQMVKDLRTNVEVNDPSKVLDGDLDGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCHHHH
LEAGIRWRMAQQQEAQA
HHHCHHHHHHHHHHCCC
>Mature Secondary Structure
MQPEVSADLSDLDGTLTTIEKVLDLDELGDRARELEEKSADPSLWDDPDHAQQVTSELSR
CCCCCCCCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VQSKLKKVKGLRQRLDDLPVLYDMAEEEAGDDPDAAQEAVQLADTEREELRQEIESLEVT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TMLSGEYDEREAVLNIRSAAGGVDAADWAEMLLRMYTRWAEKAGHKVEVYDISYAEEAGI
HEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCC
KSATIVIHGEYMYGTLSVEQGTHRLVRISPFDNQGRRQTSFAEVEVLPVVEQTDHIDIDE
CEEEEEEEECEEEEEEEECCCCCEEEEECCCCCCCCCCCCHHEEEEEEEECCCCCCCCCC
NDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGIVVTCQNEKSQIQNKASAMRVLQAKLL
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ERKRQEEQAEMDALKGDGSNSWGNQMRNYVLHPYQMVKDLRTNVEVNDPSKVLDGDLDGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCHHHH
LEAGIRWRMAQQQEAQA
HHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA