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The map label for this gene is pbuG [H]

Identifier: 68535491

GI number: 68535491

Start: 504972

End: 506465

Strand: Direct

Name: pbuG [H]

Synonym: jk0421

Alternate gene names: 68535491

Gene position: 504972-506465 (Clockwise)

Preceding gene: 68535490

Following gene: 68535492

Centisome position: 20.51

GC content: 61.04

Gene sequence:

>1494_bases
ATGGCAGATGCACAGGCCGCAAAGGCCGCAGAGACAAAATCTGGGAAACCGCAGGACCGTGGCTTCCTAGACCGCTTTTT
CCATATTTCGGAGCGCCACTCGACGGTGGGCACCGAGGTCCGCGGTGGTGTGGTCACGTTCTTCGCGATGGCCTACATCA
TCATCCTGAACCCGTTGATCCTGGGCACCAGTGCAGACTTCAACGGCGACGAGCTGGGCACCGCCCGCGTAGCTGCGGTT
ACCGCCCTGGCGGCCGGTATCATGACCATCGCCTTCGGCATCATCGCCCGCTATCCCTTCGGCATCGCTGCCGGTCTGGG
CATTAATACCCTGGTGGCCGTCACCTTCGTCGGCACAGAGGGGCTGACCTGGCCGGAAGCCATGGGGCTGGTGGTCATCG
ACGGTATCCTCATCGTGCTGCTGGCCATCACGGGCTTCCGCGAGGCTGTGTTTAACTCCATCCCGAGCTCGCTGAAGGCG
GCCATCGGCGTGGGTATTGGCCTGTTTATTGCAATGATTGGCTTGGTGGACTCCGGCTTCGTCCGCCGCATTCCGGATGC
CGCGGGCACCACCGTCCCGGTTGGCCTGGGCATCAATGGCTCCATTGCTTCTTGGCCCACGTTCGTCTTCATTCTCGGCC
TGCTAATCTGCGGCACTCTGGTGGTCCGCAAGGTTCGCGGCGGCCTATTCATCGGTATCGTCGCCAACACCGTCATTGCC
ATGATCGTTGAGGCGCTCACTCACACCGGCCCGTCCTTCGTCGATGGCAAGCCAAACCCTGCAGGCTGGAACCTGGCGGT
GCCGCAGAGCCCGGATTCTCTGGGCGGTATGCCGGACCTTTCCCTGGTCGGCGCTGTGGATCTGTTCGGTGCGTTCAGCC
GGGTGGGCGTGCTGGCAGCGTGCATGCTGATCTTCACCCTGGTGCTGGCGAACTTCTTCGACGCTATGGGAACCATGACT
GCACTGGGCAAGCAGGCTCAGCTGGTAGATGAGGAAGATCGTCTGCCAAACCTGCGCACGGCCCTGATCGTCGAAGGTGG
CGGCGCGATTGTTGGTGGCGCGGTCTCTGGCTCGTCGAACACCGTGTTTGTGGACTCTGCGGCGGGTATTGCCGACGGCG
CCCGCACGGGTCTGGCCAATGTGGTTACTGGCCTACTGTTCATTCTGGCGATGTTCTTCACTCCTCTGTACGAGGTTGTG
CCGATCGAGGCTGCCGCCCCGGTTCTGGTGATCGTGGGTGCGATGATGATGGCACAGATTAAGGACATTGACTTTGCGCG
CTTCGATATTGCCCTGCCGGCCTTCCTGACCATCGTGGTCATGCCATTTACCTATTCCATCGCCAACGGCATCGGCGTGG
GCTTCATTACTTACGTCGTGATGGCACTAGTCGGCGGTCGTGCGAAGAAGGTCCACCCGCTGTTGTACTTGGTGGCTGTG
ATGTTCGTGGTCTACTTCGCCGCTGATCCGGTGATGGATCTGCTGCGAGGCTAG

Upstream 100 bases:

>100_bases
GGTTACTTATGACTTGTCGTTTCAAATTGTGGTGGCTACCTACGGTTCTGCTGGAGGGTGGCTCCCAAGCACAGTCCGAA
CAGGTAGGGGAGAAACCCGC

Downstream 100 bases:

>100_bases
CAATGGCCATTTTTTCGCCCATCCGGATCGATGACCCGCGTGACTCGCGCCTGGACGACTTTCGGAACCTCAACGCCTCC
GACAAGAAGTCCGGCGAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MADAQAAKAAETKSGKPQDRGFLDRFFHISERHSTVGTEVRGGVVTFFAMAYIIILNPLILGTSADFNGDELGTARVAAV
TALAAGIMTIAFGIIARYPFGIAAGLGINTLVAVTFVGTEGLTWPEAMGLVVIDGILIVLLAITGFREAVFNSIPSSLKA
AIGVGIGLFIAMIGLVDSGFVRRIPDAAGTTVPVGLGINGSIASWPTFVFILGLLICGTLVVRKVRGGLFIGIVANTVIA
MIVEALTHTGPSFVDGKPNPAGWNLAVPQSPDSLGGMPDLSLVGAVDLFGAFSRVGVLAACMLIFTLVLANFFDAMGTMT
ALGKQAQLVDEEDRLPNLRTALIVEGGGAIVGGAVSGSSNTVFVDSAAGIADGARTGLANVVTGLLFILAMFFTPLYEVV
PIEAAAPVLVIVGAMMMAQIKDIDFARFDIALPAFLTIVVMPFTYSIANGIGVGFITYVVMALVGGRAKKVHPLLYLVAV
MFVVYFAADPVMDLLRG

Sequences:

>Translated_497_residues
MADAQAAKAAETKSGKPQDRGFLDRFFHISERHSTVGTEVRGGVVTFFAMAYIIILNPLILGTSADFNGDELGTARVAAV
TALAAGIMTIAFGIIARYPFGIAAGLGINTLVAVTFVGTEGLTWPEAMGLVVIDGILIVLLAITGFREAVFNSIPSSLKA
AIGVGIGLFIAMIGLVDSGFVRRIPDAAGTTVPVGLGINGSIASWPTFVFILGLLICGTLVVRKVRGGLFIGIVANTVIA
MIVEALTHTGPSFVDGKPNPAGWNLAVPQSPDSLGGMPDLSLVGAVDLFGAFSRVGVLAACMLIFTLVLANFFDAMGTMT
ALGKQAQLVDEEDRLPNLRTALIVEGGGAIVGGAVSGSSNTVFVDSAAGIADGARTGLANVVTGLLFILAMFFTPLYEVV
PIEAAAPVLVIVGAMMMAQIKDIDFARFDIALPAFLTIVVMPFTYSIANGIGVGFITYVVMALVGGRAKKVHPLLYLVAV
MFVVYFAADPVMDLLRG
>Mature_496_residues
ADAQAAKAAETKSGKPQDRGFLDRFFHISERHSTVGTEVRGGVVTFFAMAYIIILNPLILGTSADFNGDELGTARVAAVT
ALAAGIMTIAFGIIARYPFGIAAGLGINTLVAVTFVGTEGLTWPEAMGLVVIDGILIVLLAITGFREAVFNSIPSSLKAA
IGVGIGLFIAMIGLVDSGFVRRIPDAAGTTVPVGLGINGSIASWPTFVFILGLLICGTLVVRKVRGGLFIGIVANTVIAM
IVEALTHTGPSFVDGKPNPAGWNLAVPQSPDSLGGMPDLSLVGAVDLFGAFSRVGVLAACMLIFTLVLANFFDAMGTMTA
LGKQAQLVDEEDRLPNLRTALIVEGGGAIVGGAVSGSSNTVFVDSAAGIADGARTGLANVVTGLLFILAMFFTPLYEVVP
IEAAAPVLVIVGAMMMAQIKDIDFARFDIALPAFLTIVVMPFTYSIANGIGVGFITYVVMALVGGRAKKVHPLLYLVAVM
FVVYFAADPVMDLLRG

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=466, Percent_Identity=32.618025751073, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790150, Length=475, Percent_Identity=32.4210526315789, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790499, Length=474, Percent_Identity=32.2784810126582, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI48994909, Length=472, Percent_Identity=30.2966101694915, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 51535; Mature: 51403

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAQAAKAAETKSGKPQDRGFLDRFFHISERHSTVGTEVRGGVVTFFAMAYIIILNPLI
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHH
LGTSADFNGDELGTARVAAVTALAAGIMTIAFGIIARYPFGIAAGLGINTLVAVTFVGTE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHEEEECCC
GLTWPEAMGLVVIDGILIVLLAITGFREAVFNSIPSSLKAAIGVGIGLFIAMIGLVDSGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
VRRIPDAAGTTVPVGLGINGSIASWPTFVFILGLLICGTLVVRKVRGGLFIGIVANTVIA
HHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
MIVEALTHTGPSFVDGKPNPAGWNLAVPQSPDSLGGMPDLSLVGAVDLFGAFSRVGVLAA
HHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
CMLIFTLVLANFFDAMGTMTALGKQAQLVDEEDRLPNLRTALIVEGGGAIVGGAVSGSSN
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCEEEEEEECCCEEEECEECCCCC
TVFVDSAAGIADGARTGLANVVTGLLFILAMFFTPLYEVVPIEAAAPVLVIVGAMMMAQI
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KDIDFARFDIALPAFLTIVVMPFTYSIANGIGVGFITYVVMALVGGRAKKVHPLLYLVAV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
MFVVYFAADPVMDLLRG
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADAQAAKAAETKSGKPQDRGFLDRFFHISERHSTVGTEVRGGVVTFFAMAYIIILNPLI
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHH
LGTSADFNGDELGTARVAAVTALAAGIMTIAFGIIARYPFGIAAGLGINTLVAVTFVGTE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHEEEECCC
GLTWPEAMGLVVIDGILIVLLAITGFREAVFNSIPSSLKAAIGVGIGLFIAMIGLVDSGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
VRRIPDAAGTTVPVGLGINGSIASWPTFVFILGLLICGTLVVRKVRGGLFIGIVANTVIA
HHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
MIVEALTHTGPSFVDGKPNPAGWNLAVPQSPDSLGGMPDLSLVGAVDLFGAFSRVGVLAA
HHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
CMLIFTLVLANFFDAMGTMTALGKQAQLVDEEDRLPNLRTALIVEGGGAIVGGAVSGSSN
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCEEEEEEECCCEEEECEECCCCC
TVFVDSAAGIADGARTGLANVVTGLLFILAMFFTPLYEVVPIEAAAPVLVIVGAMMMAQI
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KDIDFARFDIALPAFLTIVVMPFTYSIANGIGVGFITYVVMALVGGRAKKVHPLLYLVAV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
MFVVYFAADPVMDLLRG
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]