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The map label for this gene is purD [H]

Identifier: 68535411

GI number: 68535411

Start: 415728

End: 416984

Strand: Direct

Name: purD [H]

Synonym: jk0346

Alternate gene names: 68535411

Gene position: 415728-416984 (Clockwise)

Preceding gene: 68535407

Following gene: 68535412

Centisome position: 16.88

GC content: 65.08

Gene sequence:

>1257_bases
ATGCGCATTCTTGTTATCGGCAGCGGTGCCCGCGAGCACGCACTGGCTTATTCTCTGTCCCTGGATCCCTCCGTCGAACA
GGTCCACGTTAGCCCGGGTAACGCCGGCATGACGGCCGTGGCCACCCTGCACCCGGACGGCGATCCGGTGGCTCTAGCCC
GCGAGATCGAAGCAGACCTGGTTGTTATCGGCCCCGAGATCCCGCTGGTCGCCGGAGTGGCCGACGAGCTGCGCGCGGCG
GGCTTTGCCGTATTCGGTCCGTCGGCCGCCGCCGCACAGATCGAAGGTTCGAAGGCCTTTGCGAAGGACATTATGGCCAA
GGCGCAGGTTCGTACCGCCGATGCCGTTTCTGTTCCTGTCGGGTCTAGCGATTCTTTTATTGACGCCACCCTAGACAAAT
TCGGCCCAACTTACGTTGTCAAGGACGATGGCCTGGCTGGCGGCAAGGGTGTCGTGGTTACGGCCGATCGCGCGGAGGCT
CTGGAGCACATCCACGCCGTTCACGCCGGCGGCAACCCGGTGCTTCTGGAAAGCTTCCTGGACGGCCCCGAGGTTTCCCT
GTTCTGCCTAGTGGATGGCGAGACCGTGGTGCCGCTGCTGCCTGCACAGGATTTCAAGCGAGTGGGGGATAACGACAAAG
GCCCGAACACCGGCGGCATGGGCGCCTACACCCCGCTGCCGTGGCTGCCGGAAGACGGCGTGCAGCGCATCGTGACCGAG
GTCGTGGAGCCGGTGGCCAAGCAGATGGTCGCCGAGGGTGTGCCCTTCAACGGCCTGCTGTACGCAGGTCTGGCGTGGGG
TAGCGAAGGTCCTGCTGTGGTGGAGTTCAACTGCCGCTTCGGCGACCCGGAGACTCAGCCCGTGCTGCGTTTGCTGAAGA
CCCCGCTGGGTGGAGTGCTGAATGCTGTGGCGACCGGCACCCTGGATCAGTTGCCGCCGTTGGAGTGGGAGGACGGCTAC
GCACTGACCGTCGTGCTGGCCGCCGAGAACTATCCGGCCAGCCCGGTTACCGGCAAACCGATTGTCGGGGCGGAGCCGGG
CAACGAATCCCGGGGCATCTTGGCTGCGGGCGTGGCAGAGCAGAATGGTCAGCTGGTGAGCTCCGGCGGCCGCGTGCTGA
ACGTCATCGGCACCGGCGCCACCCTGGCGGAGGCACGCAAGCAGGCCTATGAGACCCTGCAGGGCATCCAGCTGGAAGGT
TCGCACTACCGCTCCGATATCGCTCTTCCCGCAGTTGAGGGCCGCATTTCCATCTAG

Upstream 100 bases:

>100_bases
CTCGCCGTTCAGGATTTTCGTGAATACCGTAGCCATGCTGCCGATGTTAGCGCGTTGAGCGTATCTGGTGGTGGGGGTTA
GCTTCGCTAGACTGGGAGCC

Downstream 100 bases:

>100_bases
TGCCCGAACGCTGTTTTGGCAGTATTTGGGTGTAAAGATTGCATAAGGGTTTCGCACTGGGTGCAATGGGTTTAAGGTAA
CCTTTTGTGATTGCACACCC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRILVIGSGAREHALAYSLSLDPSVEQVHVSPGNAGMTAVATLHPDGDPVALAREIEADLVVIGPEIPLVAGVADELRAA
GFAVFGPSAAAAQIEGSKAFAKDIMAKAQVRTADAVSVPVGSSDSFIDATLDKFGPTYVVKDDGLAGGKGVVVTADRAEA
LEHIHAVHAGGNPVLLESFLDGPEVSLFCLVDGETVVPLLPAQDFKRVGDNDKGPNTGGMGAYTPLPWLPEDGVQRIVTE
VVEPVAKQMVAEGVPFNGLLYAGLAWGSEGPAVVEFNCRFGDPETQPVLRLLKTPLGGVLNAVATGTLDQLPPLEWEDGY
ALTVVLAAENYPASPVTGKPIVGAEPGNESRGILAAGVAEQNGQLVSSGGRVLNVIGTGATLAEARKQAYETLQGIQLEG
SHYRSDIALPAVEGRISI

Sequences:

>Translated_418_residues
MRILVIGSGAREHALAYSLSLDPSVEQVHVSPGNAGMTAVATLHPDGDPVALAREIEADLVVIGPEIPLVAGVADELRAA
GFAVFGPSAAAAQIEGSKAFAKDIMAKAQVRTADAVSVPVGSSDSFIDATLDKFGPTYVVKDDGLAGGKGVVVTADRAEA
LEHIHAVHAGGNPVLLESFLDGPEVSLFCLVDGETVVPLLPAQDFKRVGDNDKGPNTGGMGAYTPLPWLPEDGVQRIVTE
VVEPVAKQMVAEGVPFNGLLYAGLAWGSEGPAVVEFNCRFGDPETQPVLRLLKTPLGGVLNAVATGTLDQLPPLEWEDGY
ALTVVLAAENYPASPVTGKPIVGAEPGNESRGILAAGVAEQNGQLVSSGGRVLNVIGTGATLAEARKQAYETLQGIQLEG
SHYRSDIALPAVEGRISI
>Mature_418_residues
MRILVIGSGAREHALAYSLSLDPSVEQVHVSPGNAGMTAVATLHPDGDPVALAREIEADLVVIGPEIPLVAGVADELRAA
GFAVFGPSAAAAQIEGSKAFAKDIMAKAQVRTADAVSVPVGSSDSFIDATLDKFGPTYVVKDDGLAGGKGVVVTADRAEA
LEHIHAVHAGGNPVLLESFLDGPEVSLFCLVDGETVVPLLPAQDFKRVGDNDKGPNTGGMGAYTPLPWLPEDGVQRIVTE
VVEPVAKQMVAEGVPFNGLLYAGLAWGSEGPAVVEFNCRFGDPETQPVLRLLKTPLGGVLNAVATGTLDQLPPLEWEDGY
ALTVVLAAENYPASPVTGKPIVGAEPGNESRGILAAGVAEQNGQLVSSGGRVLNVIGTGATLAEARKQAYETLQGIQLEG
SHYRSDIALPAVEGRISI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=41.8224299065421, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=41.8224299065421, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=41.8224299065421, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=41.8224299065421, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1790438, Length=439, Percent_Identity=43.0523917995444, Blast_Score=311, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17567511, Length=417, Percent_Identity=37.1702637889688, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=38.0313199105145, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI62471619, Length=439, Percent_Identity=38.9521640091116, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24582400, Length=439, Percent_Identity=38.9521640091116, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43135; Mature: 43135

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVIGSGAREHALAYSLSLDPSVEQVHVSPGNAGMTAVATLHPDGDPVALAREIEADL
CEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEECCCCE
VVIGPEIPLVAGVADELRAAGFAVFGPSAAAAQIEGSKAFAKDIMAKAQVRTADAVSVPV
EEECCCCCEEECCHHHHHHCCEEEECCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECC
GSSDSFIDATLDKFGPTYVVKDDGLAGGKGVVVTADRAEALEHIHAVHAGGNPVLLESFL
CCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEHHHC
DGPEVSLFCLVDGETVVPLLPAQDFKRVGDNDKGPNTGGMGAYTPLPWLPEDGVQRIVTE
CCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VVEPVAKQMVAEGVPFNGLLYAGLAWGSEGPAVVEFNCRFGDPETQPVLRLLKTPLGGVL
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHH
NAVATGTLDQLPPLEWEDGYALTVVLAAENYPASPVTGKPIVGAEPGNESRGILAAGVAE
HHHHCCCHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCEEEECCCC
QNGQLVSSGGRVLNVIGTGATLAEARKQAYETLQGIQLEGSHYRSDIALPAVEGRISI
CCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHCEEECCCCCCCCEECEECCCEECC
>Mature Secondary Structure
MRILVIGSGAREHALAYSLSLDPSVEQVHVSPGNAGMTAVATLHPDGDPVALAREIEADL
CEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEECCCCE
VVIGPEIPLVAGVADELRAAGFAVFGPSAAAAQIEGSKAFAKDIMAKAQVRTADAVSVPV
EEECCCCCEEECCHHHHHHCCEEEECCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECC
GSSDSFIDATLDKFGPTYVVKDDGLAGGKGVVVTADRAEALEHIHAVHAGGNPVLLESFL
CCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEHHHC
DGPEVSLFCLVDGETVVPLLPAQDFKRVGDNDKGPNTGGMGAYTPLPWLPEDGVQRIVTE
CCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VVEPVAKQMVAEGVPFNGLLYAGLAWGSEGPAVVEFNCRFGDPETQPVLRLLKTPLGGVL
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHH
NAVATGTLDQLPPLEWEDGYALTVVLAAENYPASPVTGKPIVGAEPGNESRGILAAGVAE
HHHHCCCHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCEEEECCCC
QNGQLVSSGGRVLNVIGTGATLAEARKQAYETLQGIQLEGSHYRSDIALPAVEGRISI
CCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHCEEECCCCCCCCEECEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]