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The map label for this gene is ispF

Identifier: 68535374

GI number: 68535374

Start: 377401

End: 377892

Strand: Direct

Name: ispF

Synonym: jk0309

Alternate gene names: 68535374

Gene position: 377401-377892 (Clockwise)

Preceding gene: 68535373

Following gene: 68535375

Centisome position: 15.33

GC content: 66.46

Gene sequence:

>492_bases
TTGAACACCCCACACATCATTCCCCGCGTAGGCATTGCGACGGATGCTCACCAGGTGCAGCCCGGTAAGGACTGCTGGAT
GGCTTGCCTCTTGTGGGAGGACCAGGACGGGTGCGAGGGGCACTCCGACGGCGACGTGGTGGCGCACGCGGTAGTGGATG
CGCTGCTCTCCGCCGCAGGACTGGGGGATCTGGGCAGCTTCGTCGGGGTGGGGCGCCAAGAATACGACAACGTCTCCGGT
GAGCGCCTACTGCGCGAGTGCCGGGAGCTGCTGGAATCCAAGGGCTTCGTAATCGGCAACGCGGCGGTGCAGATGGTGGG
TAACCGCCCGAAAATGGGCACCCGGCGCGAAGAGGCCGAGAAGGTGATGGGCGAGCTGATCGGCGCGCCGGTCTCCGTCT
CTGCGACCACGACCGATCACATGGGCTTCACCGGCCGCGGCGAGGGTGTGGCGGCCGTGGCGACAGCGGTCGTGTGGCAG
GCACAGAATTAG

Upstream 100 bases:

>100_bases
AGGATTACCGCGTGGCGCAGATGATCCTGAATGGCAGCGAAGGCTAGAAAGCCCCCAGCAGACAAGTAGAACGCAACCAG
AGGCGAGAGGCAGACCGCAA

Downstream 100 bases:

>100_bases
TTATTGACAATCCTTTTATGTCTTCGTAGGTTTGTTCCATCACACAGAATCTCTGCGCACTAAAGGTCTGAAACTTCCTT
GTCGAATAGCTACTCATTTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MNTPHIIPRVGIATDAHQVQPGKDCWMACLLWEDQDGCEGHSDGDVVAHAVVDALLSAAGLGDLGSFVGVGRQEYDNVSG
ERLLRECRELLESKGFVIGNAAVQMVGNRPKMGTRREEAEKVMGELIGAPVSVSATTTDHMGFTGRGEGVAAVATAVVWQ
AQN

Sequences:

>Translated_163_residues
MNTPHIIPRVGIATDAHQVQPGKDCWMACLLWEDQDGCEGHSDGDVVAHAVVDALLSAAGLGDLGSFVGVGRQEYDNVSG
ERLLRECRELLESKGFVIGNAAVQMVGNRPKMGTRREEAEKVMGELIGAPVSVSATTTDHMGFTGRGEGVAAVATAVVWQ
AQN
>Mature_163_residues
MNTPHIIPRVGIATDAHQVQPGKDCWMACLLWEDQDGCEGHSDGDVVAHAVVDALLSAAGLGDLGSFVGVGRQEYDNVSG
ERLLRECRELLESKGFVIGNAAVQMVGNRPKMGTRREEAEKVMGELIGAPVSVSATTTDHMGFTGRGEGVAAVATAVVWQ
AQN

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=34.8101265822785, Blast_Score=93, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CORJK (Q4JXJ6)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250079.1
- ProteinModelPortal:   Q4JXJ6
- SMR:   Q4JXJ6
- STRING:   Q4JXJ6
- GeneID:   3432464
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0309
- NMPDR:   fig|306537.3.peg.1123
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   CJEI306537:JK0309-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17229; Mature: 17229

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPHIIPRVGIATDAHQVQPGKDCWMACLLWEDQDGCEGHSDGDVVAHAVVDALLSAAG
CCCCCCCCCCCCCCCCHHCCCCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
LGDLGSFVGVGRQEYDNVSGERLLRECRELLESKGFVIGNAAVQMVGNRPKMGTRREEAE
CCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCCCCCHHHHH
KVMGELIGAPVSVSATTTDHMGFTGRGEGVAAVATAVVWQAQN
HHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTPHIIPRVGIATDAHQVQPGKDCWMACLLWEDQDGCEGHSDGDVVAHAVVDALLSAAG
CCCCCCCCCCCCCCCCHHCCCCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
LGDLGSFVGVGRQEYDNVSGERLLRECRELLESKGFVIGNAAVQMVGNRPKMGTRREEAE
CCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCCCCCHHHHH
KVMGELIGAPVSVSATTTDHMGFTGRGEGVAAVATAVVWQAQN
HHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA