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The map label for this gene is ispD

Identifier: 68535373

GI number: 68535373

Start: 376520

End: 377347

Strand: Direct

Name: ispD

Synonym: jk0308

Alternate gene names: 68535373

Gene position: 376520-377347 (Clockwise)

Preceding gene: 68535372

Following gene: 68535374

Centisome position: 15.29

GC content: 65.22

Gene sequence:

>828_bases
GTGACAGCGAACGTCTACGCACTCGTCGCCGCCGCCGGTAGCGGCACTCGCCTGGGTTTCGATACCCCGAAGGCTTTCGT
CGAGCTCAACGGTCGGTCGTTGCTGGAACGGTCGCTGGACGGCCTGGCGGCGTCCCAAAGCATCGAAGAAACGATCGTGC
TGGTCAGCGAGAACATGCGCGACCACGCCGAGCGCATCGTCAATGATCCCATCAACGTCGCGGAGTGGGCACCCATGACC
GTCAGCGTGGAGCTCGGCGGCGGGGAGCGGTTCGACTCTGTGTATGCGGGCCTGGTTGCGATTCAACGGCGACTAAGCGC
TGCGGACGAGGCTCCTGAGGGGCGTTTCGTAGCCGTCCACGATGCCGCCCGCTGCCTCACCCCACCCGCCATGATCGCCG
AGGTTGTAGACCGCGCGCGCCGTGGCGTCGCAGATGGTTCCTGGTACGGGGCGATCCCGGTGTTGCCGGTAGTAGACACT
ATTAAGGTTATTGACGCCCCCACCTCCGGCCCGCGCGCAGGGCAGACGGTCGTGGAGCAAACCCCCGACCGCGCTAGCCT
GCGGGCAGCGGCCACGCCACAGGTATTCGACCTGGGGAAGCTGATTGAAGCCAATGAGCAGTACCTGCGCACTACGGAAA
TCAGTTCCGCGCACGCGGTGGACCGGGTGGGTGCCTCGCCGCTGCCCCTGGTGACGGACGATGCCTCGCTGATGGAGATG
GCAGGCTTCCCGGTGGTGGCAGTGGATGCCGATCCGCTGGCGATGAAGATCACCACAGCCCAGGATTACCGCGTGGCGCA
GATGATCCTGAATGGCAGCGAAGGCTAG

Upstream 100 bases:

>100_bases
CTGCAGCCCGTGCCGCAGGCGTGGACGAGGACTCCAAGTCCGGTGGCTCCATCGATCTGGACGACGAGCTGGACGGCGAA
GACTAAGCCGGGCAGGCGCT

Downstream 100 bases:

>100_bases
AAAGCCCCCAGCAGACAAGTAGAACGCAACCAGAGGCGAGAGGCAGACCGCAATTGAACACCCCACACATCATTCCCCGC
GTAGGCATTGCGACGGATGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTANVYALVAAAGSGTRLGFDTPKAFVELNGRSLLERSLDGLAASQSIEETIVLVSENMRDHAERIVNDPINVAEWAPMT
VSVELGGGERFDSVYAGLVAIQRRLSAADEAPEGRFVAVHDAARCLTPPAMIAEVVDRARRGVADGSWYGAIPVLPVVDT
IKVIDAPTSGPRAGQTVVEQTPDRASLRAAATPQVFDLGKLIEANEQYLRTTEISSAHAVDRVGASPLPLVTDDASLMEM
AGFPVVAVDADPLAMKITTAQDYRVAQMILNGSEG

Sequences:

>Translated_275_residues
MTANVYALVAAAGSGTRLGFDTPKAFVELNGRSLLERSLDGLAASQSIEETIVLVSENMRDHAERIVNDPINVAEWAPMT
VSVELGGGERFDSVYAGLVAIQRRLSAADEAPEGRFVAVHDAARCLTPPAMIAEVVDRARRGVADGSWYGAIPVLPVVDT
IKVIDAPTSGPRAGQTVVEQTPDRASLRAAATPQVFDLGKLIEANEQYLRTTEISSAHAVDRVGASPLPLVTDDASLMEM
AGFPVVAVDADPLAMKITTAQDYRVAQMILNGSEG
>Mature_274_residues
TANVYALVAAAGSGTRLGFDTPKAFVELNGRSLLERSLDGLAASQSIEETIVLVSENMRDHAERIVNDPINVAEWAPMTV
SVELGGGERFDSVYAGLVAIQRRLSAADEAPEGRFVAVHDAARCLTPPAMIAEVVDRARRGVADGSWYGAIPVLPVVDTI
KVIDAPTSGPRAGQTVVEQTPDRASLRAAATPQVFDLGKLIEANEQYLRTTEISSAHAVDRVGASPLPLVTDDASLMEMA
GFPVVAVDADPLAMKITTAQDYRVAQMILNGSEG

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=269, Percent_Identity=28.996282527881, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CORJK (Q4JXJ7)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250078.1
- ProteinModelPortal:   Q4JXJ7
- STRING:   Q4JXJ7
- GeneID:   3432462
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0308
- NMPDR:   fig|306537.3.peg.1122
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   HATRSAC
- ProtClustDB:   PRK00155
- BioCyc:   CJEI306537:JK0308-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 29110; Mature: 28979

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANVYALVAAAGSGTRLGFDTPKAFVELNGRSLLERSLDGLAASQSIEETIVLVSENMR
CCCCEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEEEHHHHH
DHAERIVNDPINVAEWAPMTVSVELGGGERFDSVYAGLVAIQRRLSAADEAPEGRFVAVH
HHHHHHHCCCCCCHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DAARCLTPPAMIAEVVDRARRGVADGSWYGAIPVLPVVDTIKVIDAPTSGPRAGQTVVEQ
CCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEECCCCCCCCCCHHHHC
TPDRASLRAAATPQVFDLGKLIEANEQYLRTTEISSAHAVDRVGASPLPLVTDDASLMEM
CCCHHHEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCCEEECCHHHHHH
AGFPVVAVDADPLAMKITTAQDYRVAQMILNGSEG
CCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TANVYALVAAAGSGTRLGFDTPKAFVELNGRSLLERSLDGLAASQSIEETIVLVSENMR
CCCEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEEEHHHHH
DHAERIVNDPINVAEWAPMTVSVELGGGERFDSVYAGLVAIQRRLSAADEAPEGRFVAVH
HHHHHHHCCCCCCHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DAARCLTPPAMIAEVVDRARRGVADGSWYGAIPVLPVVDTIKVIDAPTSGPRAGQTVVEQ
CCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEECCCCCCCCCCHHHHC
TPDRASLRAAATPQVFDLGKLIEANEQYLRTTEISSAHAVDRVGASPLPLVTDDASLMEM
CCCHHHEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCCEEECCHHHHHH
AGFPVVAVDADPLAMKITTAQDYRVAQMILNGSEG
CCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA