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The map label for this gene is lysS [H]

Identifier: 68535359

GI number: 68535359

Start: 357265

End: 358920

Strand: Direct

Name: lysS [H]

Synonym: jk0294

Alternate gene names: 68535359

Gene position: 357265-358920 (Clockwise)

Preceding gene: 68535357

Following gene: 68535360

Centisome position: 14.51

GC content: 63.65

Gene sequence:

>1656_bases
ATGCCTCAATCGATTACCTGTCCGCCCCATGCGCTAGGCTTTGTAAGCGTGACTGACGCAGCAAATACCCCCGCACAAGA
CCTGCCCGAACAGCTACGCATCCGCCGTGAAAAGCGGCAGCGCATCCTCGAATCCGGCAAGGACGCTTACCCGGTAGAGG
TGCCACGCACCCACTCCCTGGCCGAGGTGCGCGCAAAGTGGGCAGTGAAGAAGACTGACGGTGAAACTGAAGAGCAGCCG
GTTGAGGCAGAGGGCGTCACTTACCTCGCCGCAGGCGACGAAACCCAGGACGTAGTGAGCGTGACCGGGCGCCTGATCTT
CATGCGCAACACCGGCAAGCTTTGCTTCGCCACCCTGCAGGATGGCGACGGGACGCAGCTGCAGGCCATGCTCTCCCTGG
CGGAGGTCGGCGAGGACGCACTCGCGGCCTGGAAGGCTGACGTGGACCTCGGCGACTTCATCGGTGTGACCGGTCGCGTG
ATCGCTTCCCGCCGCGGCGAGCTATCCGTCATGGCCAGCGAGTGGGTCATGGCTGCCAAGGCCCTGCGTCCGCTGCCCGT
GGCGTTCGCAGAAATGAACGAAGAAACTCGCGTGCGTCAGCGCTACAACGACCTGATCGTGCGCGAGGAGGCCCGCAAGA
ATGCAATGACGCGCGTGAAGGTCATCCGCGCGCTGCGCGACTACATGGAGGGCGAGGGCTTCGTAGAGATCGAAACCCCA
ATGCTGCAGACTCTGCACGGTGGCGCGGCGGCTCGACCGTTCGTCACGCACTCCAACGCGCTGGACATCGACCTGTACCT
GCGAATCGCGCCGGAGCTGTTCCTGAAGCGCGCCGTGGTGGGCGGCATCGACCGCGTCTTCGAGATCAACCGCAACTTCC
GTAACGAGGGCGTGGACAAGTCCCACTCCCCGGAGTTCGCAATGCTGGAGACCTACGCCGCGTGGGGTGACTACGACGAC
TGTGCCCGCACCACCCGCGAATCCATCCAGTACATCGTGGACCAGCTAACCAAGGGCACCGACCTGGAGGGCACTGGCAA
GGTGCGTCTCGCCGACGGCACCGAGTTCGACTTCGGCGGGGAGTGGAAGGAAATCGAGATGTACCCCTCGCTGAACGAGG
CACTTGCGCGCAAGTACCCCGGCCAGCCAGAGGTGACGATCGACTCCACCGTTGAGGAGCTCAAGGAAATCGCTAAGGTC
ATTGGCCTGGACGTTCCCGCTAAGGGCGGCTGGGGTCACGGCAAGCTGGTGGAGGAGATCTGGGAGGTCTTGTGCGAGGA
CCAGCTGGAAGGCCCCATCTTCGTGCGTAACTTCCCCGTAGAGACCTCCCCGCTGACCCGCCAGCACCGCAGCAAGCCGG
GTGTGACAGAGAAGTGGGATCTGTACGTCCGCGGATTCGAGCTGGCCACCGGCTACTCCGAGCTGATCGACCCGGTTATT
CAGCGCGAGCGCTTTGAGGATCAGGCACGCCTGGCGGCCAATGGCGATGATGAAGCCATGCGCCTGGACGAGGACTTCCT
GGCTGCCATGGAGCAGGGCATGCCGCCGACAGCCGGCACTGGCATGGGTATCGACCGACTGCTGATGGCCTTGACCGGTC
TGGGTATCCGCGAGACCGTGCTGTTCCCGATCGTGAAGCCGGAGCGCGATAACTAG

Upstream 100 bases:

>100_bases
GTTATGGCTGGTGAAACTTACTGACTGGTGGAAGTTACCTAGGAGTAAGGGGAACAAAACGTCTTATGTTCCCTAAAGCT
AACACCCCCGGGCGACCTGC

Downstream 100 bases:

>100_bases
ACGCCTATCAACGGCAGTAGATTGTGGTGGGATCTACTGCCGGGGCCGGTTGGCTCGAGGGGGAGAACTAGAGGGAGTTT
TTCGGGGGTGGCTCGGGGGC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MPQSITCPPHALGFVSVTDAANTPAQDLPEQLRIRREKRQRILESGKDAYPVEVPRTHSLAEVRAKWAVKKTDGETEEQP
VEAEGVTYLAAGDETQDVVSVTGRLIFMRNTGKLCFATLQDGDGTQLQAMLSLAEVGEDALAAWKADVDLGDFIGVTGRV
IASRRGELSVMASEWVMAAKALRPLPVAFAEMNEETRVRQRYNDLIVREEARKNAMTRVKVIRALRDYMEGEGFVEIETP
MLQTLHGGAAARPFVTHSNALDIDLYLRIAPELFLKRAVVGGIDRVFEINRNFRNEGVDKSHSPEFAMLETYAAWGDYDD
CARTTRESIQYIVDQLTKGTDLEGTGKVRLADGTEFDFGGEWKEIEMYPSLNEALARKYPGQPEVTIDSTVEELKEIAKV
IGLDVPAKGGWGHGKLVEEIWEVLCEDQLEGPIFVRNFPVETSPLTRQHRSKPGVTEKWDLYVRGFELATGYSELIDPVI
QRERFEDQARLAANGDDEAMRLDEDFLAAMEQGMPPTAGTGMGIDRLLMALTGLGIRETVLFPIVKPERDN

Sequences:

>Translated_551_residues
MPQSITCPPHALGFVSVTDAANTPAQDLPEQLRIRREKRQRILESGKDAYPVEVPRTHSLAEVRAKWAVKKTDGETEEQP
VEAEGVTYLAAGDETQDVVSVTGRLIFMRNTGKLCFATLQDGDGTQLQAMLSLAEVGEDALAAWKADVDLGDFIGVTGRV
IASRRGELSVMASEWVMAAKALRPLPVAFAEMNEETRVRQRYNDLIVREEARKNAMTRVKVIRALRDYMEGEGFVEIETP
MLQTLHGGAAARPFVTHSNALDIDLYLRIAPELFLKRAVVGGIDRVFEINRNFRNEGVDKSHSPEFAMLETYAAWGDYDD
CARTTRESIQYIVDQLTKGTDLEGTGKVRLADGTEFDFGGEWKEIEMYPSLNEALARKYPGQPEVTIDSTVEELKEIAKV
IGLDVPAKGGWGHGKLVEEIWEVLCEDQLEGPIFVRNFPVETSPLTRQHRSKPGVTEKWDLYVRGFELATGYSELIDPVI
QRERFEDQARLAANGDDEAMRLDEDFLAAMEQGMPPTAGTGMGIDRLLMALTGLGIRETVLFPIVKPERDN
>Mature_550_residues
PQSITCPPHALGFVSVTDAANTPAQDLPEQLRIRREKRQRILESGKDAYPVEVPRTHSLAEVRAKWAVKKTDGETEEQPV
EAEGVTYLAAGDETQDVVSVTGRLIFMRNTGKLCFATLQDGDGTQLQAMLSLAEVGEDALAAWKADVDLGDFIGVTGRVI
ASRRGELSVMASEWVMAAKALRPLPVAFAEMNEETRVRQRYNDLIVREEARKNAMTRVKVIRALRDYMEGEGFVEIETPM
LQTLHGGAAARPFVTHSNALDIDLYLRIAPELFLKRAVVGGIDRVFEINRNFRNEGVDKSHSPEFAMLETYAAWGDYDDC
ARTTRESIQYIVDQLTKGTDLEGTGKVRLADGTEFDFGGEWKEIEMYPSLNEALARKYPGQPEVTIDSTVEELKEIAKVI
GLDVPAKGGWGHGKLVEEIWEVLCEDQLEGPIFVRNFPVETSPLTRQHRSKPGVTEKWDLYVRGFELATGYSELIDPVIQ
RERFEDQARLAANGDDEAMRLDEDFLAAMEQGMPPTAGTGMGIDRLLMALTGLGIRETVLFPIVKPERDN

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=535, Percent_Identity=38.6915887850467, Blast_Score=334, Evalue=2e-91,
Organism=Homo sapiens, GI194272210, Length=535, Percent_Identity=38.6915887850467, Blast_Score=333, Evalue=3e-91,
Organism=Escherichia coli, GI1790571, Length=530, Percent_Identity=40.377358490566, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1789256, Length=523, Percent_Identity=39.961759082218, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI87082379, Length=339, Percent_Identity=27.1386430678466, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788173, Length=260, Percent_Identity=30, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=549, Percent_Identity=35.8834244080146, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17535927, Length=549, Percent_Identity=35.8834244080146, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=492, Percent_Identity=37.3983739837398, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32566633, Length=207, Percent_Identity=28.5024154589372, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320242, Length=467, Percent_Identity=39.8286937901499, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=28.1512605042017, Blast_Score=170, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24640849, Length=537, Percent_Identity=39.292364990689, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640851, Length=537, Percent_Identity=39.292364990689, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24584738, Length=285, Percent_Identity=28.7719298245614, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 61390; Mature: 61259

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSITCPPHALGFVSVTDAANTPAQDLPEQLRIRREKRQRILESGKDAYPVEVPRTHSL
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
AEVRAKWAVKKTDGETEEQPVEAEGVTYLAAGDETQDVVSVTGRLIFMRNTGKLCFATLQ
HHHHHHHEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCEEEEEECCCCEEEEEEC
DGDGTQLQAMLSLAEVGEDALAAWKADVDLGDFIGVTGRVIASRRGELSVMASEWVMAAK
CCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCHHHHHHCCCCCEEEHHHHHHHHHH
ALRPLPVAFAEMNEETRVRQRYNDLIVREEARKNAMTRVKVIRALRDYMEGEGFVEIETP
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCH
MLQTLHGGAAARPFVTHSNALDIDLYLRIAPELFLKRAVVGGIDRVFEINRNFRNEGVDK
HHHHHCCCCCCCCEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SHSPEFAMLETYAAWGDYDDCARTTRESIQYIVDQLTKGTDLEGTGKVRLADGTEFDFGG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCC
EWKEIEMYPSLNEALARKYPGQPEVTIDSTVEELKEIAKVIGLDVPAKGGWGHGKLVEEI
CCEEEEECCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
WEVLCEDQLEGPIFVRNFPVETSPLTRQHRSKPGVTEKWDLYVRGFELATGYSELIDPVI
HHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCEEEEEEEEEHHCCHHHHHHHHH
QRERFEDQARLAANGDDEAMRLDEDFLAAMEQGMPPTAGTGMGIDRLLMALTGLGIRETV
HHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHE
LFPIVKPERDN
EEEEECCCCCC
>Mature Secondary Structure 
PQSITCPPHALGFVSVTDAANTPAQDLPEQLRIRREKRQRILESGKDAYPVEVPRTHSL
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
AEVRAKWAVKKTDGETEEQPVEAEGVTYLAAGDETQDVVSVTGRLIFMRNTGKLCFATLQ
HHHHHHHEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCEEEEEECCCCEEEEEEC
DGDGTQLQAMLSLAEVGEDALAAWKADVDLGDFIGVTGRVIASRRGELSVMASEWVMAAK
CCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHCCHHHHHHCCCCCEEEHHHHHHHHHH
ALRPLPVAFAEMNEETRVRQRYNDLIVREEARKNAMTRVKVIRALRDYMEGEGFVEIETP
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCH
MLQTLHGGAAARPFVTHSNALDIDLYLRIAPELFLKRAVVGGIDRVFEINRNFRNEGVDK
HHHHHCCCCCCCCEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SHSPEFAMLETYAAWGDYDDCARTTRESIQYIVDQLTKGTDLEGTGKVRLADGTEFDFGG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCC
EWKEIEMYPSLNEALARKYPGQPEVTIDSTVEELKEIAKVIGLDVPAKGGWGHGKLVEEI
CCEEEEECCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
WEVLCEDQLEGPIFVRNFPVETSPLTRQHRSKPGVTEKWDLYVRGFELATGYSELIDPVI
HHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCEEEEEEEEEHHCCHHHHHHHHH
QRERFEDQARLAANGDDEAMRLDEDFLAAMEQGMPPTAGTGMGIDRLLMALTGLGIRETV
HHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHE
LFPIVKPERDN
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA