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The map label for this gene is purT

Identifier: 68535304

GI number: 68535304

Start: 295771

End: 297006

Strand: Direct

Name: purT

Synonym: jk0239

Alternate gene names: 68535304

Gene position: 295771-297006 (Clockwise)

Preceding gene: 68535302

Following gene: 68535305

Centisome position: 12.01

GC content: 65.53

Gene sequence:

>1236_bases
ATGTACACTCCCGAGGCAATTGGCACTCCACTGACTCCCTCCTCCCTCAAGGTTCTCCTGCTCGGCGCCAGCGAGCTGGG
GCGGGAGCTCGCCATCGCCCTGCAACGCCTGGGCATAGAGGTGCACGCTGCGGACCGCTACGCCGGCGCGCCCGCGCACC
TCGTGGCCAACAAGGCCCACGTGCTGGACATCCACGACGAGGACGCCATCCGCCAGCTGGTTCGCGAGGTTGGGCCGCAC
TTCGTCATCCCGGATTCGGAGACCATCCCCGCCGAAGTGCTGCAGGAGATCGAGACCAACGAGGTCGCCTCCGTCGTGCC
GACCGCCCTGGCCAATCGCCTGACCATGAACCGCGAAGGCATCCGCACCTTCGCCCACGACAAGCTGGGCCTGCCGAACA
GCACCTTTCGCTTCGCCTCCACCGCCGACGAACTTGCCCGCGCCGCCGAGGAAATCGGCTACCCGTGCGTGGTCAAGCCC
GTCATGAGCAGCTCCGGCGCAGGCCAAAGCTACGTTGGGGGAGTGGACGAACTGGAGGCCGCTTGGAATGCAGCACTGAA
GCACTCCCGCACCAACGCCCAGCGGGTAATGGTCGAGCAGTACATCCCCTTCGACTACGAAGTCACCATCCTGGCCGTAC
GCTCCATCGACCCCGCTACCGGCCGGGAGGCCACCTGGTTCTGCGAGCCCATCGGCCACCGCCAAGACCGCGGCGACTAC
GTGGAATCCTGGCAGCCCGCGCACATGTCCGAGGATGCGCTGGATACCGCCCGCAGCATCGCTGCCCGCATCGCCACCGC
CCTGGGAGGCCGCGGAGTGTACGGCGTGGAACTGTTCGTCAAGGGCGACGATGTCTACTTCTCTGAGGTCAGCCCCCGCC
TGCACGATACCGGCATGGTCACCCTCGGCACCCAGCGGTTCTCTGAATTCGACCTGCACGCACGCGCCATCCTTGGGCTG
CCGATCGATACCACGCTGATCTCTCCGGGAGCCTCCGCCGTTATCCGCTGCGAGGATAAACACGACGGCGATATTGAATA
CGCGGGCGTCAATAAGGCAATGGCAGTAGAAGAAACCAACATCTACCTATTCGGCAAGCCCCGAGCACTCGCGCGCCGGC
GAATGGGAGTGGCAGTAGCCACCGCCGAGAACATCGACCAAGCCCGGCAGCGCGCCGAAGAGGCCGCCGGGTACATCGAG
GTGCGGCCGACGGTTTTCGCAGAAGGCGGGCAGTAG

Upstream 100 bases:

>100_bases
TCAGGCCGCACCGGCTACAAGCTCGCGCGCGGCGGGGCTGGCACACAAGTGCGGCGGGGCTGGCAGCGTGAGTATGTGGG
CATAAGTAAGGTGAAAGACT

Downstream 100 bases:

>100_bases
ACCTAAAATCCGAGGTATGGGACATAGCGCAACCGACCTCAACGAAACTCGAATCCTGCTGTACTACTGCTTCACCCCGA
TCGACGACCCGACCGCGGTG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MYTPEAIGTPLTPSSLKVLLLGASELGRELAIALQRLGIEVHAADRYAGAPAHLVANKAHVLDIHDEDAIRQLVREVGPH
FVIPDSETIPAEVLQEIETNEVASVVPTALANRLTMNREGIRTFAHDKLGLPNSTFRFASTADELARAAEEIGYPCVVKP
VMSSSGAGQSYVGGVDELEAAWNAALKHSRTNAQRVMVEQYIPFDYEVTILAVRSIDPATGREATWFCEPIGHRQDRGDY
VESWQPAHMSEDALDTARSIAARIATALGGRGVYGVELFVKGDDVYFSEVSPRLHDTGMVTLGTQRFSEFDLHARAILGL
PIDTTLISPGASAVIRCEDKHDGDIEYAGVNKAMAVEETNIYLFGKPRALARRRMGVAVATAENIDQARQRAEEAAGYIE
VRPTVFAEGGQ

Sequences:

>Translated_411_residues
MYTPEAIGTPLTPSSLKVLLLGASELGRELAIALQRLGIEVHAADRYAGAPAHLVANKAHVLDIHDEDAIRQLVREVGPH
FVIPDSETIPAEVLQEIETNEVASVVPTALANRLTMNREGIRTFAHDKLGLPNSTFRFASTADELARAAEEIGYPCVVKP
VMSSSGAGQSYVGGVDELEAAWNAALKHSRTNAQRVMVEQYIPFDYEVTILAVRSIDPATGREATWFCEPIGHRQDRGDY
VESWQPAHMSEDALDTARSIAARIATALGGRGVYGVELFVKGDDVYFSEVSPRLHDTGMVTLGTQRFSEFDLHARAILGL
PIDTTLISPGASAVIRCEDKHDGDIEYAGVNKAMAVEETNIYLFGKPRALARRRMGVAVATAENIDQARQRAEEAAGYIE
VRPTVFAEGGQ
>Mature_411_residues
MYTPEAIGTPLTPSSLKVLLLGASELGRELAIALQRLGIEVHAADRYAGAPAHLVANKAHVLDIHDEDAIRQLVREVGPH
FVIPDSETIPAEVLQEIETNEVASVVPTALANRLTMNREGIRTFAHDKLGLPNSTFRFASTADELARAAEEIGYPCVVKP
VMSSSGAGQSYVGGVDELEAAWNAALKHSRTNAQRVMVEQYIPFDYEVTILAVRSIDPATGREATWFCEPIGHRQDRGDY
VESWQPAHMSEDALDTARSIAARIATALGGRGVYGVELFVKGDDVYFSEVSPRLHDTGMVTLGTQRFSEFDLHARAILGL
PIDTTLISPGASAVIRCEDKHDGDIEYAGVNKAMAVEETNIYLFGKPRALARRRMGVAVATAENIDQARQRAEEAAGYIE
VRPTVFAEGGQ

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=397, Percent_Identity=49.1183879093199, Blast_Score=371, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324702, Length=267, Percent_Identity=24.7191011235955, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_CORJK (Q4JXR6)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250009.1
- HSSP:   P33221
- ProteinModelPortal:   Q4JXR6
- SMR:   Q4JXR6
- STRING:   Q4JXR6
- GeneID:   3431906
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0239
- NMPDR:   fig|306537.3.peg.671
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   CJEI306537:JK0239-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44630; Mature: 44630

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 118-118 BINDING 159-159 BINDING 207-207 BINDING 277-277 BINDING 289-289 BINDING 296-296 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTPEAIGTPLTPSSLKVLLLGASELGRELAIALQRLGIEVHAADRYAGAPAHLVANKAH
CCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHCCCCEE
VLDIHDEDAIRQLVREVGPHFVIPDSETIPAEVLQEIETNEVASVVPTALANRLTMNREG
EEEECCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IRTFAHDKLGLPNSTFRFASTADELARAAEEIGYPCVVKPVMSSSGAGQSYVGGVDELEA
HHHHHHHCCCCCCCCEEHHCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHCCCHHHHHH
AWNAALKHSRTNAQRVMVEQYIPFDYEVTILAVRSIDPATGREATWFCEPIGHRQDRGDY
HHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCH
VESWQPAHMSEDALDTARSIAARIATALGGRGVYGVELFVKGDDVYFSEVSPRLHDTGMV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TLGTQRFSEFDLHARAILGLPIDTTLISPGASAVIRCEDKHDGDIEYAGVNKAMAVEETN
EECCHHHHHHCCEEEEEECCCCCCEEECCCCCEEEEECCCCCCCEEEECCCCEEEEEECE
IYLFGKPRALARRRMGVAVATAENIDQARQRAEEAAGYIEVRPTVFAEGGQ
EEEEECCHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCEEEECCEEEECCCC
>Mature Secondary Structure
MYTPEAIGTPLTPSSLKVLLLGASELGRELAIALQRLGIEVHAADRYAGAPAHLVANKAH
CCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHCCCCEE
VLDIHDEDAIRQLVREVGPHFVIPDSETIPAEVLQEIETNEVASVVPTALANRLTMNREG
EEEECCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IRTFAHDKLGLPNSTFRFASTADELARAAEEIGYPCVVKPVMSSSGAGQSYVGGVDELEA
HHHHHHHCCCCCCCCEEHHCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHCCCHHHHHH
AWNAALKHSRTNAQRVMVEQYIPFDYEVTILAVRSIDPATGREATWFCEPIGHRQDRGDY
HHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCH
VESWQPAHMSEDALDTARSIAARIATALGGRGVYGVELFVKGDDVYFSEVSPRLHDTGMV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TLGTQRFSEFDLHARAILGLPIDTTLISPGASAVIRCEDKHDGDIEYAGVNKAMAVEETN
EECCHHHHHHCCEEEEEECCCCCCEEECCCCCEEEEECCCCCCCEEEECCCCEEEEEECE
IYLFGKPRALARRRMGVAVATAENIDQARQRAEEAAGYIEVRPTVFAEGGQ
EEEEECCHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCEEEECCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA