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The map label for this gene is purA

Identifier: 68535287

GI number: 68535287

Start: 276092

End: 277381

Strand: Direct

Name: purA

Synonym: jk0222

Alternate gene names: 68535287

Gene position: 276092-277381 (Clockwise)

Preceding gene: 68535285

Following gene: 68535289

Centisome position: 11.21

GC content: 64.65

Gene sequence:

>1290_bases
ATGGCAGCGATCATCGTCGTAGGCGCCCAGTGGGGCGACGAAGGTAAGGGCAAAGCTACCGACATTCTGGGCGGAAAGGT
CGACTACGTAGTCAAGCCCAACGGCGGCAACAACGCCGGCCACACCGTCGTCGTCGGCGGCGAAAAGTACGAGCTCAAGC
TGCTGCCCGCAGGCGTGCTTTCCGAAAACGCCACGCCCATCATCGGCAACGGTTGCGTGGTGAACCTGGAGGCACTGTTC
GAGGAGATCGACGGGCTGGAAGCTCGCGGCGCAAACGCCTCCCGCCTGCGCGTTTCCGCCAACGCACAGCTGGTGGCGCC
CTACCACGTGGCCATCGACCGCGTGGCCGAGCGCTTCTTAGGCAAGCGCGCCATCGGCACCACCGGCCGCGGCATCGGCC
CGACCTACGCCGACAAGGTCTCCCGCGTGGGTATCCGCGCCCAGGACCTGCTGGACGAATCCATCCTGCGCCAGAAGATC
GAATCCGCGCTGGACCAGAAGAACCAGATCCTCGTGAAGATCTACAACCGTCGCGCCGTCGACGTGGACGAGGTCGTGGA
ATATTTCCTCTCCTACGCCGATCGCCTCAAGCCGATGCTGATCGATGCGACGACGGAGCTGAATAAGGCTCTGGACGCCG
GTAAGTCCGTGCTGATGGAGGGCGGCCAGGCCACGATGCTAGACGTGGACCACGGCACGTATCCCTTCGTGACTTCCTCT
AACCCCACCACCGGCGGTGCGTGCGTGGGTACGGGCATTGGGCCGACGCGCATTACCGCATCGCTGGGCATCATCAAGGC
CTACACCACGCGCGTGGGTGCCGGTCCGTTCCCCACGGAGCTGTTCGACAAGTGGGGCGAGTTCCTGCAGACCACCGGCG
GCGAGGTCGGTGTGAACACCGGACGCAAGCGCCGCTGCGGCTGGTACGACTCCGTGATCGCCCGCTACGCCTCCCGCGTA
AATGGCTTCACCGACTACTTCCTGACGAAGCTGGACGTGCTGACCGGCATCGGCGAAATCCCGATCTGTGTGGCCTACGA
CGTGGACGGTGTGCGCCACGACGAGATGCCGATGACGCAGACCGAGTTCCACCACGCCACCCCGATCTACGAGACCATGC
CCGCGTGGGACGAGGACATCACCGACTGCAAGACCTTCGAGGAGCTGCCAGAGAAGGCGCAGGATTACGTCAAGCGCCTG
GAGGAGCTGTCCGGCTGCCGGATCTCCTACATCGGCGTGGGCCCGGGCCGCGACCAGACGATCGTCATCAACGACGTCGC
GGAGGGCTAG

Upstream 100 bases:

>100_bases
CGATCTCTACCCGAAGCTACAGTAGATGCAGCAGATGCTTGGAGCGGGGCTACACCCATGAGTACAGAAGTACCAACGCA
GAGGACGTGTTTTAGTTTCC

Downstream 100 bases:

>100_bases
GCCTAGCGGCGCTAGAGTGCGCATCGCTGGCCGGCTTCATGCGGCAGCGGCCTGTGCACTTAGGTGTGGCCGGGGGGGTG
TCCCTATGTTTTTGTCAAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAAIIVVGAQWGDEGKGKATDILGGKVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSENATPIIGNGCVVNLEALF
EEIDGLEARGANASRLRVSANAQLVAPYHVAIDRVAERFLGKRAIGTTGRGIGPTYADKVSRVGIRAQDLLDESILRQKI
ESALDQKNQILVKIYNRRAVDVDEVVEYFLSYADRLKPMLIDATTELNKALDAGKSVLMEGGQATMLDVDHGTYPFVTSS
NPTTGGACVGTGIGPTRITASLGIIKAYTTRVGAGPFPTELFDKWGEFLQTTGGEVGVNTGRKRRCGWYDSVIARYASRV
NGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPMTQTEFHHATPIYETMPAWDEDITDCKTFEELPEKAQDYVKRL
EELSGCRISYIGVGPGRDQTIVINDVAEG

Sequences:

>Translated_429_residues
MAAIIVVGAQWGDEGKGKATDILGGKVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSENATPIIGNGCVVNLEALF
EEIDGLEARGANASRLRVSANAQLVAPYHVAIDRVAERFLGKRAIGTTGRGIGPTYADKVSRVGIRAQDLLDESILRQKI
ESALDQKNQILVKIYNRRAVDVDEVVEYFLSYADRLKPMLIDATTELNKALDAGKSVLMEGGQATMLDVDHGTYPFVTSS
NPTTGGACVGTGIGPTRITASLGIIKAYTTRVGAGPFPTELFDKWGEFLQTTGGEVGVNTGRKRRCGWYDSVIARYASRV
NGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPMTQTEFHHATPIYETMPAWDEDITDCKTFEELPEKAQDYVKRL
EELSGCRISYIGVGPGRDQTIVINDVAEG
>Mature_428_residues
AAIIVVGAQWGDEGKGKATDILGGKVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSENATPIIGNGCVVNLEALFE
EIDGLEARGANASRLRVSANAQLVAPYHVAIDRVAERFLGKRAIGTTGRGIGPTYADKVSRVGIRAQDLLDESILRQKIE
SALDQKNQILVKIYNRRAVDVDEVVEYFLSYADRLKPMLIDATTELNKALDAGKSVLMEGGQATMLDVDHGTYPFVTSSN
PTTGGACVGTGIGPTRITASLGIIKAYTTRVGAGPFPTELFDKWGEFLQTTGGEVGVNTGRKRRCGWYDSVIARYASRVN
GFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPMTQTEFHHATPIYETMPAWDEDITDCKTFEELPEKAQDYVKRLE
ELSGCRISYIGVGPGRDQTIVINDVAEG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=43.3962264150943, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=42.9577464788732, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=42.6767676767677, Blast_Score=315, Evalue=7e-86,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=48.1132075471698, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=42.6573426573427, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=42.6573426573427, Blast_Score=334, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=43.1554524361949, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.8679245283019, Blast_Score=354, Evalue=8e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CORJK (Q4JXT3)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_249992.1
- ProteinModelPortal:   Q4JXT3
- SMR:   Q4JXT3
- STRING:   Q4JXT3
- GeneID:   3432998
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0222
- NMPDR:   fig|306537.3.peg.531
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   CJEI306537:JK0222-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46383; Mature: 46252

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIIVVGAQWGDEGKGKATDILGGKVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVL
CEEEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEECCCEEEEEEECCHHH
SENATPIIGNGCVVNLEALFEEIDGLEARGANASRLRVSANAQLVAPYHVAIDRVAERFL
CCCCCEEECCCCEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHH
GKRAIGTTGRGIGPTYADKVSRVGIRAQDLLDESILRQKIESALDQKNQILVKIYNRRAV
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
DVDEVVEYFLSYADRLKPMLIDATTELNKALDAGKSVLMEGGQATMLDVDHGTYPFVTSS
CHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCHHHHHCCCCEEEEECCCCCCCEEECC
NPTTGGACVGTGIGPTRITASLGIIKAYTTRVGAGPFPTELFDKWGEFLQTTGGEVGVNT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRKRRCGWYDSVIARYASRVNGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPMTQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCH
TEFHHATPIYETMPAWDEDITDCKTFEELPEKAQDYVKRLEELSGCRISYIGVGPGRDQT
HHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
IVINDVAEG
EEEECCCCC
>Mature Secondary Structure 
AAIIVVGAQWGDEGKGKATDILGGKVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVL
EEEEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEECCCEEEEEEECCHHH
SENATPIIGNGCVVNLEALFEEIDGLEARGANASRLRVSANAQLVAPYHVAIDRVAERFL
CCCCCEEECCCCEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHH
GKRAIGTTGRGIGPTYADKVSRVGIRAQDLLDESILRQKIESALDQKNQILVKIYNRRAV
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
DVDEVVEYFLSYADRLKPMLIDATTELNKALDAGKSVLMEGGQATMLDVDHGTYPFVTSS
CHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCHHHHHCCCCEEEEECCCCCCCEEECC
NPTTGGACVGTGIGPTRITASLGIIKAYTTRVGAGPFPTELFDKWGEFLQTTGGEVGVNT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRKRRCGWYDSVIARYASRVNGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPMTQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCH
TEFHHATPIYETMPAWDEDITDCKTFEELPEKAQDYVKRLEELSGCRISYIGVGPGRDQT
HHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
IVINDVAEG
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA