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The map label for this gene is folD [H]

Identifier: 66768254

GI number: 66768254

Start: 2333066

End: 2333950

Strand: Direct

Name: folD [H]

Synonym: XC_1933

Alternate gene names: 66768254

Gene position: 2333066-2333950 (Clockwise)

Preceding gene: 66768252

Following gene: 66768255

Centisome position: 45.31

GC content: 69.04

Gene sequence:

>885_bases
ATGACCGCTCCCGCGCCAGCTGCCCTCGCTCCGGCCCGCCTCCTCGATGGTCGCCGCATTGCCGAGGAATTGCTCGACGG
TCTGAAGCTGCGCGTGGATGCGCGCCTGGCGGCGGGCAAGACCCGGCCCGGGCTGGCGGTGGTGCTGGTAGGCGGCGATC
CGGCTTCGTCGGTGTATGTGCGCAACAAGCGCCGTGCGGCCGAAAAAGTGGGCATCGAGGCCTTCGACTACGACCTGCCA
CAAGGCACCACCGAAGCCGAGCTGGCCGCGTTGATCGACCAGCTCAACACCGATCCCAAGATCCACGGCATCCTGATCCA
GTTGCCGCTGCCGGGCATTCCCGATGCCAACCGGTTGATCCAGCGCATCGACCCGCGCAAGGACGTGGACGGGTTCCATC
CGCAGAACGTCGGGCATCTGGCGCTGCGCGAATTCGGTTTGCGGCCGTGTACCCCGCGCGGCATCGTGACCTTGCTGGCG
CATACCGACCAGCCGGTGCGCGGGCGCAACGCCACCATCGTCGGGGTGAGTAACCATGTGGGCCGCCCGATGGGGCTGGA
GCTGCTGATTGCCGGGTGCACGGTGACCAGCTGCCACAAGTTCACCCCGCCGGATGTGCTGGAAGCCAGCGTGCGCAACG
CCGACATCCTGGTGGTGGCGGTGGGCCGGCCGGGGCTGATCCCGGGCGAATGGGTCAAGCCTGGGGCGGTGGTCATCGAC
GTGGGCATCAACCGCCTGGACGATGGGCGCCTGGTCGGCGATGTGGGGTTCGAGGCCGCAGCGCAGCGCGCCGGCTGGAT
CACCCCGGTGCCGGGCGGGGTGGGGCCGATGACGGTGGCCACGCTGATGCAGAACACGCTCGAGGCGGCCGATGCGGCTG
GTTGA

Upstream 100 bases:

>100_bases
CTGCAGCAGGCGGCGGAACGCGGCGGCTTCGATCGAAGTGGTGTCGGTCATGGCGGAATCGGGGACAATAGACGGCCTTC
GACTCTACACCTGACGCCCC

Downstream 100 bases:

>100_bases
GCCGTTGGTGTAGCGCAGCATTGCGGCGGCACTCAGCTGCCGCAGTGTCTTCGCGGCCGCTTTCAGGGTAAAATGTCGCG
CTTCCCCAAACTCGGGTCCG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTAPAPAALAPARLLDGRRIAEELLDGLKLRVDARLAAGKTRPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QGTTEAELAALIDQLNTDPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLA
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFEAAAQRAGWITPVPGGVGPMTVATLMQNTLEAADAAG

Sequences:

>Translated_294_residues
MTAPAPAALAPARLLDGRRIAEELLDGLKLRVDARLAAGKTRPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QGTTEAELAALIDQLNTDPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLA
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFEAAAQRAGWITPVPGGVGPMTVATLMQNTLEAADAAG
>Mature_293_residues
TAPAPAALAPARLLDGRRIAEELLDGLKLRVDARLAAGKTRPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLPQ
GTTEAELAALIDQLNTDPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLAH
TDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVIDV
GINRLDDGRLVGDVGFEAAAQRAGWITPVPGGVGPMTVATLMQNTLEAADAAG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=44.3686006825939, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=44.1780821917808, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=43.4931506849315, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI36796743, Length=208, Percent_Identity=25.4807692307692, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=59.4982078853047, Blast_Score=331, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=43.75, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=41.8918918918919, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=41.6083916083916, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322933, Length=323, Percent_Identity=26.0061919504644, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=48.972602739726, Blast_Score=247, Evalue=6e-66,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=48.972602739726, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI62472483, Length=299, Percent_Identity=42.4749163879599, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45551871, Length=299, Percent_Identity=42.4749163879599, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=42.8082191780822, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=42.8082191780822, Blast_Score=211, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30932; Mature: 30801

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPAPAALAPARLLDGRRIAEELLDGLKLRVDARLAAGKTRPGLAVVLVGGDPASSVYV
CCCCCCCHHHHHHHHCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQGTTEAELAALIDQLNTDPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLAHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHEEEEEECCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFEAAAQRAGWITPVPGGVGPMTVATLMQNTLEAADAAG
ECCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAPAPAALAPARLLDGRRIAEELLDGLKLRVDARLAAGKTRPGLAVVLVGGDPASSVYV
CCCCCCHHHHHHHHCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQGTTEAELAALIDQLNTDPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLAHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHEEEEEECCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFEAAAQRAGWITPVPGGVGPMTVATLMQNTLEAADAAG
ECCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]