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The map label for this gene is rluB [H]

Identifier: 66768225

GI number: 66768225

Start: 2299205

End: 2300833

Strand: Reverse

Name: rluB [H]

Synonym: XC_1904

Alternate gene names: 66768225

Gene position: 2300833-2299205 (Counterclockwise)

Preceding gene: 66768226

Following gene: 66768223

Centisome position: 44.69

GC content: 71.03

Gene sequence:

>1629_bases
ATGAGTGACACCCCCCGCAAGCTGTCGTTGAACAAGCTTTCCCTCAAGCGCGACAGCGCGCCCGAAGCGCCGAAACTGGA
AGAGCGCCTGCACAAGGTGCTGGCCCAGGCCGGCCTGGGCTCGCGCCGCGCGCTGGAGCAGCGCATTGCCGATGGCCTGA
TCAAGGTCAATGGCGCCGTCGCGCAGACCGGCATGTCGGTGCGCAGCGGCGACAAGATCGAACTGGACGGCCGCAGCTTC
GTCGCCAGCGCGCTGACCGAATCCTCGCGCGTGCTGGTCTACAACAAGCCCGAAGGCGAAGTGACCACCCGCGAAGATCC
CGAAGGCCGCCCGACCGTATTCGAGTCGCTGCCGGCGCTGAAGGGCTCGCGCTGGATCGCTATCGGCCGCCTGGACATCA
ACACCACCGGCCTGCTGCTGCTCACCACCGATGGCGAGCTGGCCAACGCGATGATGCACCCCTCCTACGAGGTTGAACGC
GAGTACGTGGTGCGCGTGCGCGCCCCGGAAGGCGAAGAGAAGGTGCCCGACAGCATGATCGAGCGGCTCTCGCGCGGCGT
GTTGCTGGAAGACGGCGGCGCCAAGTTCGACGAGATCGAACGCATCGGTGGCACCGATTCGCACGACTGGTTCCGCGTGG
TGGTCAAGGAAGGCCGTAACCGCGAAGTGCGCCGCCTGTGGGAGTCGCAAGGCTGCCAGGTCAGCCGCCTCAAGCGCACC
CGCTACGGCAAGGTGTCGCTGCCACGCGAGCTGTTGCGCGGGCAATCGGTGGAACTGGCACAGGAAAAGGTCGACGCCCT
GCGCGCCGAACTCAAGCTGGAAGAAGGCGCGCCGTCGGCACTGACGCTGCAGCCGGTGATCGGCCAGCGCCGCGCCGCCA
AGTCGACCGTGCACGTGTCGCGCGACGGCCGCAGCAATGCCTACGTCAACGGCCAGACCTCCGGCGCCGATGAAGGCCGT
GAGTTGCGCCGCTTCGACAACCTGCGCGAGGATCGCGGCGGCCGTGGTGGCCGCGGCAAGCCCGGCGGCTTCAAGGGCGG
CCTGACGGTCAGCGGCGAAGCCGCTGCCAAGCAATCGCAGCAGCGCCCGTTCAAGCAGCGTGGCCCGGCCAAGGGCGACC
GCTCGCTGCCGGACGGCAACCCGGCCGCGTTCCGCAGCTGGTATGTGCCCGATGGCGTGAGCACCGGCCCGAGCGGCCAC
CTGCAACGCAGGTCCGGGCGGCGCCGGCACCGGCCAGCCGCGTCCCTACGCCAAGAAGGGTCCGGGCGGTGCACGCCCGG
GCGCTGGCGGCCAGGCCGGCCGCGAGTGTGGGCAAGGTCAGTCGCAGGGTCAAGGTCAAGGCCAGCGCAACGACCCCTAC
GGCCACCCGGGCAACGCGCCGAGCTTCCCGTCCGATCACGCCAACCCGGGTTTCAGCCCGTATGGCGCCGCACGGCGGCC
ACCGGTCGTCCAACCGGCGCCCGCCCCGGCGGCAACCGTGGCCCGAATGCCAACCGCGGCCCCGGTGGCCCGGGTGGTGC
CGCACGTGGACCGGGCGGTCCTGGCGGCGCACCGCGCGGCGGCCCGGGCGGCCGTCCGCCGGGCGGCGGCAACCGTCGGC
CACCAGGTGGCGGCAATCGCGGTCGCTAA

Upstream 100 bases:

>100_bases
GAAGACGAAGACAACGCCGTCGCGACGACGACCGTGGCTGTTGACGAAGCCGATTCCGACCCAGAGGCCGACCCAGAACG
CGTCGGCCGGAGCCAGACAC

Downstream 100 bases:

>100_bases
GCTACAAGACGTGCAATAAAAAAACGGCCCTGCGAAGGGCCGTTTTTCGTTATCGCTTGGATCATGTCTGCTTGAATCAC
GCGATACATCGAAGTAAGCG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAVAQTGMSVRSGDKIELDGRSF
VASALTESSRVLVYNKPEGEVTTREDPEGRPTVFESLPALKGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVER
EYVVRVRAPEGEEKVPDSMIERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRT
RYGKVSLPRELLRGQSVELAQEKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVSRDGRSNAYVNGQTSGADEGR
ELRRFDNLREDRGGRGGRGKPGGFKGGLTVSGEAAAKQSQQRPFKQRGPAKGDRSLPDGNPAAFRSWYVPDGVSTGPSGH
LQRRSGRRRHRPAASLRQEGSGRCTPGRWRPGRPRVWARSVAGSRSRPAQRPLRPPGQRAELPVRSRQPGFQPVWRRTAA
TGRPTGARPGGNRGPNANRGPGGPGGAARGPGGPGGAPRGGPGGRPPGGGNRRPPGGGNRGR

Sequences:

>Translated_542_residues
MSDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAVAQTGMSVRSGDKIELDGRSF
VASALTESSRVLVYNKPEGEVTTREDPEGRPTVFESLPALKGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVER
EYVVRVRAPEGEEKVPDSMIERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRT
RYGKVSLPRELLRGQSVELAQEKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVSRDGRSNAYVNGQTSGADEGR
ELRRFDNLREDRGGRGGRGKPGGFKGGLTVSGEAAAKQSQQRPFKQRGPAKGDRSLPDGNPAAFRSWYVPDGVSTGPSGH
LQRRSGRRRHRPAASLRQEGSGRCTPGRWRPGRPRVWARSVAGSRSRPAQRPLRPPGQRAELPVRSRQPGFQPVWRRTAA
TGRPTGARPGGNRGPNANRGPGGPGGAARGPGGPGGAPRGGPGGRPPGGGNRRPPGGGNRGR
>Mature_541_residues
SDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAVAQTGMSVRSGDKIELDGRSFV
ASALTESSRVLVYNKPEGEVTTREDPEGRPTVFESLPALKGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVERE
YVVRVRAPEGEEKVPDSMIERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRTR
YGKVSLPRELLRGQSVELAQEKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVSRDGRSNAYVNGQTSGADEGRE
LRRFDNLREDRGGRGGRGKPGGFKGGLTVSGEAAAKQSQQRPFKQRGPAKGDRSLPDGNPAAFRSWYVPDGVSTGPSGHL
QRRSGRRRHRPAASLRQEGSGRCTPGRWRPGRPRVWARSVAGSRSRPAQRPLRPPGQRAELPVRSRQPGFQPVWRRTAAT
GRPTGARPGGNRGPNANRGPGGPGGAARGPGGPGGAPRGGPGGRPPGGGNRRPPGGGNRGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=53.7848605577689, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1790453, Length=219, Percent_Identity=32.8767123287671, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788510, Length=240, Percent_Identity=31.25, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=32.5714285714286, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 58577; Mature: 58446

Theoretical pI: Translated: 11.80; Mature: 11.80

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAV
CCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCHH
AQTGMSVRSGDKIELDGRSFVASALTESSRVLVYNKPEGEVTTREDPEGRPTVFESLPAL
HHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCC
KGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVEREYVVRVRAPEGEEKVPDSMI
CCCCEEEEEEEEECCCEEEEEECCCHHHHHHHCCCHHCCCEEEEEEECCCCCCCCCHHHH
ERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRT
HHHHCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHH
RYGKVSLPRELLRGQSVELAQEKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVS
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHCCEEEEE
RDGRSNAYVNGQTSGADEGRELRRFDNLREDRGGRGGRGKPGGFKGGLTVSGEAAAKQSQ
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHH
QRPFKQRGPAKGDRSLPDGNPAAFRSWYVPDGVSTGPSGHLQRRSGRRRHRPAASLRQEG
CCCHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCCCHHHHHCCCHHCCCHHHHHHCC
SGRCTPGRWRPGRPRVWARSVAGSRSRPAQRPLRPPGQRAELPVRSRQPGFQPVWRRTAA
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
TGRPTGARPGGNRGPNANRGPGGPGGAARGPGGPGGAPRGGPGGRPPGGGNRRPPGGGNR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GR
CC
>Mature Secondary Structure 
SDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAV
CCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCHH
AQTGMSVRSGDKIELDGRSFVASALTESSRVLVYNKPEGEVTTREDPEGRPTVFESLPAL
HHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCC
KGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVEREYVVRVRAPEGEEKVPDSMI
CCCCEEEEEEEEECCCEEEEEECCCHHHHHHHCCCHHCCCEEEEEEECCCCCCCCCHHHH
ERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRT
HHHHCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHH
RYGKVSLPRELLRGQSVELAQEKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVS
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHCCEEEEE
RDGRSNAYVNGQTSGADEGRELRRFDNLREDRGGRGGRGKPGGFKGGLTVSGEAAAKQSQ
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHH
QRPFKQRGPAKGDRSLPDGNPAAFRSWYVPDGVSTGPSGHLQRRSGRRRHRPAASLRQEG
CCCHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCCCHHHHHCCCHHCCCHHHHHHCC
SGRCTPGRWRPGRPRVWARSVAGSRSRPAQRPLRPPGQRAELPVRSRQPGFQPVWRRTAA
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
TGRPTGARPGGNRGPNANRGPGGPGGAARGPGGPGGAPRGGPGGRPPGGGNRRPPGGGNR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]