Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 66768095

GI number: 66768095

Start: 2157895

End: 2159481

Strand: Direct

Name: emrB [H]

Synonym: XC_1774

Alternate gene names: 66768095

Gene position: 2157895-2159481 (Clockwise)

Preceding gene: 66768094

Following gene: 66768096

Centisome position: 41.91

GC content: 64.78

Gene sequence:

>1587_bases
ATGAATGCCGCGGCGGCCACCGGCGAGCGTGCCGGCGGCAGCGCCGGACGCGAGAAAGCCGAACCGGGCGCGTGGCTGGC
GGTACTGGCCGGCACCATCGGCTCGTTCATGGCGACGCTGGACATCTCCATCGTCAACGCCGCACTGCCCACCATCCAGG
GCGAAGTGGGCGCCAGCGGCACCGAGGGCACCTGGATCTCCACCGCCTACCTGGTGGCGGAAATCATCATGATTCCGCTC
ACCGGCTGGTTCGTGCGCACGCTGGGCCTGCGTAACTTCCTGCTGATCTGCGCGGTGATGTTTACTGCTTTCTCGGTGGT
CTGCGGGCTGTCCACCTCGCTGACCATGATGATCATCGGCCGCGTGGGCCAGGGCCTGGCCGGCGGTGCGTTGATCCCTA
CCGCGCTGACCATTGTCGCCACACGCTTGCCGCCCAGCCAGCAGACCATGGGCACCGCCTTGTTCGGCATGACCGTGATC
ATGGGCCCGGTGATCGGCCCGCTGCTCGGTGGCTGGCTGACCGAAAACGTCAGCTGGCACTACGCGTTTTTCATCAACGT
GCCGATCTGCGTGGGCTTGGTGGCGCTGTTGCTGCTCGGGCTCAAGCACGAAAAAGGCAACTGGGCCGGCCTGCTGGATG
CGGACTGGCTGGGCATCTACGGGCTCACCGCCGGCCTGGGCGGGCTCACCGTGGTGCTGGAAGAAGGCCAGCGCGAGCGC
TGGTTCGAATCCAGCGAAATCAACACGCTCAGCGTATTCGCGCTGAGTGGATTTTTGGCGCTGGTGATCTCGCAGTTTGG
CCAGCGCGCACCGGTGATCCGGCTGTCGCTGCTGCTGCACCGCAGCTTCGGCGCGGTGTTCATCATGATCATGGCGGTGG
GCATGATCCTGTTCGGGGTCATGTACATGATCCCGCAGTTCCTGTCGGTGATTTCCGGCTACAACACCGAACAGGCCGGC
TATGTGTTGCTGCTGTCCGGCCTGCCCACGGTGTTGTTGATGCCGATGATGCCCAAGCTGCTGGAGGTGGTGGACGTGCG
CATCCTGGTGATCGCCGGGCTGCTGTGTTTTGCCGCGGCATGCTTCGTCAACCTGTCGCTGACCGCCGACACCGTGGGCA
TGCATTTTGTCGCCGGGCAGTTGTTGCAGGGCTGCGGCCTGGCGCTGGCGATGATGTCGCTCAACCAGGCGGCCATTTCC
TCGGTGCCGCCCGAGCTGGCCGGCGATGCGTCCGGCCTGTTCAACGCCGGCCGCAACCTGGGTGGCTCGGTTGGATTGGC
GCTGATCTCCACCTTCCAGGAGCGGCGCATGACCTTCCACACCGAGACCATCGGCAGCGCCGTCACTGCAAACGCCAGCC
GCGCGCAGGATTTCCTGGCCGGGTTGAACGCGCAGATGCAGGGCAGCGCCGGTGGCGAGGCGGCGATTCGCGCGGTTGCC
CAACTTGCGCGCCTGGTGCAACAGCAGGCGCTGGTGATGACCTATAGCGACCTGTTCTGGATCTTTGGCGTGATCGTGGT
CTGCACGATTCCGCTGGCCTTTTTGCTCAAGCCGTTACCCAAGGGGACGCACCTTGCAATGCACTGA

Upstream 100 bases:

>100_bases
GCATGTCGGTGGAGGTCACCGTGGATACCCGCTCGGCCAAGGACGCCAAGCAGCGCGCCGAGGAGGAATCGGACCGCGTG
CAGGCGCAGGAGCGCGCGCG

Downstream 100 bases:

>100_bases
TTCCCTGCGCCTGGCGCGCATGCCGCTGGCGGCAGCGCTGGCCTCACTGCTGCTGGGCGGCTGCATGCTCGGCCCCAACT
ACACCAAGCCGCCCGCGGTG

Product: transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MNAAAATGERAGGSAGREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASGTEGTWISTAYLVAEIIMIPL
TGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLTMMIIGRVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVI
MGPVIGPLLGGWLTENVSWHYAFFINVPICVGLVALLLLGLKHEKGNWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
WFESSEINTLSVFALSGFLALVISQFGQRAPVIRLSLLLHRSFGAVFIMIMAVGMILFGVMYMIPQFLSVISGYNTEQAG
YVLLLSGLPTVLLMPMMPKLLEVVDVRILVIAGLLCFAAACFVNLSLTADTVGMHFVAGQLLQGCGLALAMMSLNQAAIS
SVPPELAGDASGLFNAGRNLGGSVGLALISTFQERRMTFHTETIGSAVTANASRAQDFLAGLNAQMQGSAGGEAAIRAVA
QLARLVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGTHLAMH

Sequences:

>Translated_528_residues
MNAAAATGERAGGSAGREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASGTEGTWISTAYLVAEIIMIPL
TGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLTMMIIGRVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVI
MGPVIGPLLGGWLTENVSWHYAFFINVPICVGLVALLLLGLKHEKGNWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
WFESSEINTLSVFALSGFLALVISQFGQRAPVIRLSLLLHRSFGAVFIMIMAVGMILFGVMYMIPQFLSVISGYNTEQAG
YVLLLSGLPTVLLMPMMPKLLEVVDVRILVIAGLLCFAAACFVNLSLTADTVGMHFVAGQLLQGCGLALAMMSLNQAAIS
SVPPELAGDASGLFNAGRNLGGSVGLALISTFQERRMTFHTETIGSAVTANASRAQDFLAGLNAQMQGSAGGEAAIRAVA
QLARLVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGTHLAMH
>Mature_528_residues
MNAAAATGERAGGSAGREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASGTEGTWISTAYLVAEIIMIPL
TGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLTMMIIGRVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVI
MGPVIGPLLGGWLTENVSWHYAFFINVPICVGLVALLLLGLKHEKGNWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
WFESSEINTLSVFALSGFLALVISQFGQRAPVIRLSLLLHRSFGAVFIMIMAVGMILFGVMYMIPQFLSVISGYNTEQAG
YVLLLSGLPTVLLMPMMPKLLEVVDVRILVIAGLLCFAAACFVNLSLTADTVGMHFVAGQLLQGCGLALAMMSLNQAAIS
SVPPELAGDASGLFNAGRNLGGSVGLALISTFQERRMTFHTETIGSAVTANASRAQDFLAGLNAQMQGSAGGEAAIRAVA
QLARLVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGTHLAMH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=507, Percent_Identity=32.5443786982249, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788710, Length=497, Percent_Identity=30.9859154929577, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=27.2076372315036, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790195, Length=352, Percent_Identity=28.125, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=27.2506082725061, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6322958, Length=213, Percent_Identity=27.2300469483568, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=28.8590604026846, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=167, Percent_Identity=26.9461077844311, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55486; Mature: 55486

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAAATGERAGGSAGREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEGTWISTAYLVAEIIMIPLTGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLTMMIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVIMGPVIGPLLGGWLTENVSWH
HHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
YAFFINVPICVGLVALLLLGLKHEKGNWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
EEEEECCHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCHHH
WFESSEINTLSVFALSGFLALVISQFGQRAPVIRLSLLLHRSFGAVFIMIMAVGMILFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYMIPQFLSVISGYNTEQAGYVLLLSGLPTVLLMPMMPKLLEVVDVRILVIAGLLCFAAA
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFVNLSLTADTVGMHFVAGQLLQGCGLALAMMSLNQAAISSVPPELAGDASGLFNAGRNL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCCC
GGSVGLALISTFQERRMTFHTETIGSAVTANASRAQDFLAGLNAQMQGSAGGEAAIRAVA
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QLARLVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGTHLAMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNAAAATGERAGGSAGREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEGTWISTAYLVAEIIMIPLTGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLTMMIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVIMGPVIGPLLGGWLTENVSWH
HHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
YAFFINVPICVGLVALLLLGLKHEKGNWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
EEEEECCHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCHHH
WFESSEINTLSVFALSGFLALVISQFGQRAPVIRLSLLLHRSFGAVFIMIMAVGMILFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYMIPQFLSVISGYNTEQAGYVLLLSGLPTVLLMPMMPKLLEVVDVRILVIAGLLCFAAA
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFVNLSLTADTVGMHFVAGQLLQGCGLALAMMSLNQAAISSVPPELAGDASGLFNAGRNL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCCC
GGSVGLALISTFQERRMTFHTETIGSAVTANASRAQDFLAGLNAQMQGSAGGEAAIRAVA
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QLARLVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGTHLAMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]