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The map label for this gene is ogt [H]

Identifier: 66767790

GI number: 66767790

Start: 1765410

End: 1765952

Strand: Reverse

Name: ogt [H]

Synonym: XC_1464

Alternate gene names: 66767790

Gene position: 1765952-1765410 (Counterclockwise)

Preceding gene: 66767791

Following gene: 66767789

Centisome position: 34.3

GC content: 69.43

Gene sequence:

>543_bases
ATGAGCACGCTGCACTACGACACCTTCCCCTCGCCGATTGGTGCGCTGACCGTGGCGGCCGACACCACCGGCGTGCGCCA
CATCCTGTTCGCGCAGAATCGCCACGATGCCCCCGGGCGCGCACTCTGGCAGCACGGTCCCGACGCGCCGCTGGTGCAGG
CTGCACGCGAGCAGCTGCTGGATTATCTGTATGGCGGGCGGCGCAGCTTCGACCTGCCACTGGCCCCGGCCGGCACGCCA
TTCCAGTTGCAGGTCTGGCAGACGCTGGCCCGCATTCCGTTCGGTGAAACCTGGAGTTACGCGCAACTGGCGCAGGCAGT
GGGCCGGCCGGCTGCCAGCCGTGCGGTGGGCGCCGCAAACGGCCGCAATCCGCTGCCGATCGTGCTGCCCTGTCACCGCG
TCATCGGTGCCAGTGGCGCACTGACCGGATTCGGTGGCGGGCTGCCGACCAAACAGGCCTTGCTGCAGCTGGAAGGCTGG
TCGCCGCAGGCGTCGGCGCGCCGGGTTGCAGCCGTGGTTGGCGAAGATCTGTTTGCCCGCTGA

Upstream 100 bases:

>100_bases
AACGTGCTACCGAAGCGCGCTCGCAGGCATGGCGGCCCTGGCGCGCCTACGCCGTGCTGCACCTGTGGCATCTTGCCGTC
GACCGAAAGGACACCCGCTC

Downstream 100 bases:

>100_bases
GCTGGGCGAGTGCCGCCGCCGTTCGCGTACGCCGTGGGTGTGCATGTGGTCGCATCTGCGCGCGCGCATTGCCACACACG
AGCGCAACGTAGATCGCCTC

Product: 6-O-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSTLHYDTFPSPIGALTVAADTTGVRHILFAQNRHDAPGRALWQHGPDAPLVQAAREQLLDYLYGGRRSFDLPLAPAGTP
FQLQVWQTLARIPFGETWSYAQLAQAVGRPAASRAVGAANGRNPLPIVLPCHRVIGASGALTGFGGGLPTKQALLQLEGW
SPQASARRVAAVVGEDLFAR

Sequences:

>Translated_180_residues
MSTLHYDTFPSPIGALTVAADTTGVRHILFAQNRHDAPGRALWQHGPDAPLVQAAREQLLDYLYGGRRSFDLPLAPAGTP
FQLQVWQTLARIPFGETWSYAQLAQAVGRPAASRAVGAANGRNPLPIVLPCHRVIGASGALTGFGGGLPTKQALLQLEGW
SPQASARRVAAVVGEDLFAR
>Mature_179_residues
STLHYDTFPSPIGALTVAADTTGVRHILFAQNRHDAPGRALWQHGPDAPLVQAAREQLLDYLYGGRRSFDLPLAPAGTPF
QLQVWQTLARIPFGETWSYAQLAQAVGRPAASRAVGAANGRNPLPIVLPCHRVIGASGALTGFGGGLPTKQALLQLEGWS
PQASARRVAAVVGEDLFAR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=171, Percent_Identity=35.0877192982456, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=53.3333333333333, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788542, Length=92, Percent_Identity=50, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=48.1481481481481, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=48.1481481481481, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=36.6666666666667, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137554, Length=155, Percent_Identity=34.8387096774194, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19154; Mature: 19023

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLHYDTFPSPIGALTVAADTTGVRHILFAQNRHDAPGRALWQHGPDAPLVQAAREQLL
CCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHH
DYLYGGRRSFDLPLAPAGTPFQLQVWQTLARIPFGETWSYAQLAQAVGRPAASRAVGAAN
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCC
GRNPLPIVLPCHRVIGASGALTGFGGGLPTKQALLQLEGWSPQASARRVAAVVGEDLFAR
CCCCCEEEEECHHHHCCCCCCCCCCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLHYDTFPSPIGALTVAADTTGVRHILFAQNRHDAPGRALWQHGPDAPLVQAAREQLL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHH
DYLYGGRRSFDLPLAPAGTPFQLQVWQTLARIPFGETWSYAQLAQAVGRPAASRAVGAAN
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCC
GRNPLPIVLPCHRVIGASGALTGFGGGLPTKQALLQLEGWSPQASARRVAAVVGEDLFAR
CCCCCEEEEECHHHHCCCCCCCCCCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]