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The map label for this gene is nfi

Identifier: 66767729

GI number: 66767729

Start: 1692181

End: 1692891

Strand: Reverse

Name: nfi

Synonym: XC_1403

Alternate gene names: 66767729

Gene position: 1692891-1692181 (Counterclockwise)

Preceding gene: 66767733

Following gene: 66767728

Centisome position: 32.88

GC content: 69.62

Gene sequence:

>711_bases
ATGCAGACATCCATCGACCCCGTGTTCGCCGGTTGGGACGGCAGCGTCGCTCAGGCGCGACAGTTGCAGCAGCAACTGGC
CCAGCGCGTGGCGCTGCGCGATGAGGTTTCCGCCGCGCCTGCGCTGCTGGCCGGCTTCGATGTGGGCTTCGAAGACGATG
GCCAGACCACGCGCGCGGCGGCCGTGCTGCTGGATGCGCAGACGCTGCTGCCGCTGGAAACCCACGTCGCCCGCGTGCCG
ACCTCGATGCCGTATGTGCCCGGCCTGCTGAGTTTTCGCGAGCTGCCCGCATTGCTGCGCGCGCTTGCGCTGCTGGCGCG
CACGCCGGACCTGGTGTTCATCGATGGCCAGGGCATCGCGCATCCGCGCAGGTTCGGCATCGCCGCGCACTTTGGCGTGG
TCACTGGCCTGCCCAGCATCGGCGTGGCCAAGCAACGCCTGGCCGGAACCTTCATCGAGCCCGGTGGGGAACGCGGCGAC
CACAGCCCCATCCTGCTGGCCGGTGCGCAGATCGGCTGGGCACTGCGCAGCAAGCCGCGCTGCAATCCGTTGATCGTCTC
GCCCGGTCATCGCGTGTCGATGCAAGGCGCACTCGACTGGACCCTGCGCACGCTGCGCGCCTACCGCCTGCCCGAACCGA
CGCGGCTGGCCGACCGGCTGGCTTCACGGCGTGGCGAAATCGAGCTGCAGACGCAGCCGACGTTGCTGTAG

Upstream 100 bases:

>100_bases
GGTTCTCCGATTGGGGGCCGATCAACCGCTGATTGTAGCGGCAGCCGATGCCTGCCTGGCGTGCACGGCATCGCCACGGC
GTGCGCGCGATGCTGCGGCC

Downstream 100 bases:

>100_bases
CTCGCGTGCTGCGGGGCAAGCGTGCAATTGCGGGCATCGATGCATGGTTCGTTGGATGTGGCTTGTCAGTTCTGGCGTGT
GGATTCTGACTTCAAGCTTC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MQTSIDPVFAGWDGSVAQARQLQQQLAQRVALRDEVSAAPALLAGFDVGFEDDGQTTRAAAVLLDAQTLLPLETHVARVP
TSMPYVPGLLSFRELPALLRALALLARTPDLVFIDGQGIAHPRRFGIAAHFGVVTGLPSIGVAKQRLAGTFIEPGGERGD
HSPILLAGAQIGWALRSKPRCNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEIELQTQPTLL

Sequences:

>Translated_236_residues
MQTSIDPVFAGWDGSVAQARQLQQQLAQRVALRDEVSAAPALLAGFDVGFEDDGQTTRAAAVLLDAQTLLPLETHVARVP
TSMPYVPGLLSFRELPALLRALALLARTPDLVFIDGQGIAHPRRFGIAAHFGVVTGLPSIGVAKQRLAGTFIEPGGERGD
HSPILLAGAQIGWALRSKPRCNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEIELQTQPTLL
>Mature_236_residues
MQTSIDPVFAGWDGSVAQARQLQQQLAQRVALRDEVSAAPALLAGFDVGFEDDGQTTRAAAVLLDAQTLLPLETHVARVP
TSMPYVPGLLSFRELPALLRALALLARTPDLVFIDGQGIAHPRRFGIAAHFGVVTGLPSIGVAKQRLAGTFIEPGGERGD
HSPILLAGAQIGWALRSKPRCNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEIELQTQPTLL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=205, Percent_Identity=37.0731707317073, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI257467548, Length=121, Percent_Identity=40.495867768595, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI257467550, Length=121, Percent_Identity=40.495867768595, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87082357, Length=210, Percent_Identity=52.3809523809524, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_XANC8 (Q4UWV2)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_242491.1
- ProteinModelPortal:   Q4UWV2
- STRING:   Q4UWV2
- GeneID:   3382340
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_1403
- eggNOG:   COG1515
- HOGENOM:   HBG716360
- OMA:   MPYIPGL
- PhylomeDB:   Q4UWV2
- ProtClustDB:   PRK11617
- BioCyc:   XCAM314565:XC_1403-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25453; Mature: 25453

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSIDPVFAGWDGSVAQARQLQQQLAQRVALRDEVSAAPALLAGFDVGFEDDGQTTRAA
CCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AVLLDAQTLLPLETHVARVPTSMPYVPGLLSFRELPALLRALALLARTPDLVFIDGQGIA
HHEECHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
HPRRFGIAAHFGVVTGLPSIGVAKQRLAGTFIEPGGERGDHSPILLAGAQIGWALRSKPR
CHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCC
CNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEIELQTQPTLL
CCEEEECCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MQTSIDPVFAGWDGSVAQARQLQQQLAQRVALRDEVSAAPALLAGFDVGFEDDGQTTRAA
CCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AVLLDAQTLLPLETHVARVPTSMPYVPGLLSFRELPALLRALALLARTPDLVFIDGQGIA
HHEECHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
HPRRFGIAAHFGVVTGLPSIGVAKQRLAGTFIEPGGERGDHSPILLAGAQIGWALRSKPR
CHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCC
CNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEIELQTQPTLL
CCEEEECCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA