Click here to switch to the map view.

The map label for this gene is apbE [H]

Identifier: 66767662

GI number: 66767662

Start: 1621753

End: 1622673

Strand: Reverse

Name: apbE [H]

Synonym: XC_1335

Alternate gene names: 66767662

Gene position: 1622673-1621753 (Counterclockwise)

Preceding gene: 66767663

Following gene: 66767661

Centisome position: 31.52

GC content: 68.62

Gene sequence:

>921_bases
ATGGGCACCACCTGGAGCGTGAAAATGGTGGCGCCGCGCGGCCGCGATCTGCATCCGTTGCATGCGGTGATCCAGGGTGC
GCTCGATCGCGTGGTCGCGCAGATGAGCACCTGGGACGCAGACTCGGACATCACCCGCTTCAACCGTGCCGCCGCCGGCA
CCTGGCAGCGGTTGCCGGACGCGTTCCACCGTGTGCTGACCACGGCGCTGGAGATTGCGCGTGCCAGCGATGGCGCGTTC
GATCCAACCGTCGGCCCGATGGTAGACCTGTGGGGCTTCGGTGCCGCCGGTGGGCCGCGCAAGGTGCCCAGTGCCGAGCA
GATCGCCCTGGCCCGGCAGCGCTGTGGCTGGCAGCGCCTGCAACTGGACGCAGACAGAGCACTGCAACCCGGCGCGCTTG
CATTGGATTTGTCGGGCATCGCCAAGGGCTTCGGTGTGGACTGCGTGCAGCGCGCGTTGGCGCAGGCGGGCATCGCCAGC
GCGTTGATCGATGTCGGCGGCGAGCTGTTTGGATACGGCCGCAAACCCGATGGCAGCGCATGGCGTGTCCTGGTGGAATC
AGCGCCCGACGAAGACGCAGGCACCGCACTGCCCGCGCGCGTGCTGGCACTGGATGGACTGGCCGTGGCCACCTCCGGCG
ACCGCTGGCACCGCTTCGAGGCCGGCGGCGTACGTTACGCGCATACCTTCGACCCACGCCTGGGCGCGCCGGTTGCGCAG
GCAGCGGCCGCGGTCACCGTGCTGGCGCACGATGCGATGCATGCCGATGCCTGGGCAACCGCAATGACCGTGCTCGGTGT
TGACGCGGGCCTGGCTTATGCGACGAACGCAGGGCTTGCGGTGCGCTTTTTGCAGCGCCGCGGCGCGGATTTGCAGGAAT
CCATGAGCCCGGCGTTTGAAACGCATCTGGCTGCCGCATGA

Upstream 100 bases:

>100_bases
GTCACCATGGCTGCCTCAAGGCAGCGCCTGCTCGACCGGTCAACACATGCACCCAGTTTCTTCCGCAGATGACACCGTTG
CCACGCTGGGCGGCCGCAGC

Downstream 100 bases:

>100_bases
GCCTCGCCTCCCCACGCGTGTGGCTTGGCAACGGCGTCATCCTGCTGGCCTTGGCGGTACTGGCCGCCTGGTTGGGCAGC
CTGCATGCGGGCCCGTGGTG

Product: thiamine biosynthesis lipoprotein ApbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MGTTWSVKMVAPRGRDLHPLHAVIQGALDRVVAQMSTWDADSDITRFNRAAAGTWQRLPDAFHRVLTTALEIARASDGAF
DPTVGPMVDLWGFGAAGGPRKVPSAEQIALARQRCGWQRLQLDADRALQPGALALDLSGIAKGFGVDCVQRALAQAGIAS
ALIDVGGELFGYGRKPDGSAWRVLVESAPDEDAGTALPARVLALDGLAVATSGDRWHRFEAGGVRYAHTFDPRLGAPVAQ
AAAAVTVLAHDAMHADAWATAMTVLGVDAGLAYATNAGLAVRFLQRRGADLQESMSPAFETHLAAA

Sequences:

>Translated_306_residues
MGTTWSVKMVAPRGRDLHPLHAVIQGALDRVVAQMSTWDADSDITRFNRAAAGTWQRLPDAFHRVLTTALEIARASDGAF
DPTVGPMVDLWGFGAAGGPRKVPSAEQIALARQRCGWQRLQLDADRALQPGALALDLSGIAKGFGVDCVQRALAQAGIAS
ALIDVGGELFGYGRKPDGSAWRVLVESAPDEDAGTALPARVLALDGLAVATSGDRWHRFEAGGVRYAHTFDPRLGAPVAQ
AAAAVTVLAHDAMHADAWATAMTVLGVDAGLAYATNAGLAVRFLQRRGADLQESMSPAFETHLAAA
>Mature_305_residues
GTTWSVKMVAPRGRDLHPLHAVIQGALDRVVAQMSTWDADSDITRFNRAAAGTWQRLPDAFHRVLTTALEIARASDGAFD
PTVGPMVDLWGFGAAGGPRKVPSAEQIALARQRCGWQRLQLDADRALQPGALALDLSGIAKGFGVDCVQRALAQAGIASA
LIDVGGELFGYGRKPDGSAWRVLVESAPDEDAGTALPARVLALDGLAVATSGDRWHRFEAGGVRYAHTFDPRLGAPVAQA
AAAVTVLAHDAMHADAWATAMTVLGVDAGLAYATNAGLAVRFLQRRGADLQESMSPAFETHLAAA

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=310, Percent_Identity=33.8709677419355, Blast_Score=174, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 32164; Mature: 32032

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTWSVKMVAPRGRDLHPLHAVIQGALDRVVAQMSTWDADSDITRFNRAAAGTWQRLPD
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AFHRVLTTALEIARASDGAFDPTVGPMVDLWGFGAAGGPRKVPSAEQIALARQRCGWQRL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHEE
QLDADRALQPGALALDLSGIAKGFGVDCVQRALAQAGIASALIDVGGELFGYGRKPDGSA
EECHHHCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCC
WRVLVESAPDEDAGTALPARVLALDGLAVATSGDRWHRFEAGGVRYAHTFDPRLGAPVAQ
EEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEECCCCEEEEEECCCCCCCHHHH
AAAAVTVLAHDAMHADAWATAMTVLGVDAGLAYATNAGLAVRFLQRRGADLQESMSPAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCHHHHHCHHHH
THLAAA
HHHCCC
>Mature Secondary Structure 
GTTWSVKMVAPRGRDLHPLHAVIQGALDRVVAQMSTWDADSDITRFNRAAAGTWQRLPD
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AFHRVLTTALEIARASDGAFDPTVGPMVDLWGFGAAGGPRKVPSAEQIALARQRCGWQRL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHEE
QLDADRALQPGALALDLSGIAKGFGVDCVQRALAQAGIASALIDVGGELFGYGRKPDGSA
EECHHHCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCC
WRVLVESAPDEDAGTALPARVLALDGLAVATSGDRWHRFEAGGVRYAHTFDPRLGAPVAQ
EEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEECCCCEEEEEECCCCCCCHHHH
AAAAVTVLAHDAMHADAWATAMTVLGVDAGLAYATNAGLAVRFLQRRGADLQESMSPAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCHHHHHCHHHH
THLAAA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]