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The map label for this gene is rmlA2 [C]

Identifier: 66767655

GI number: 66767655

Start: 1614668

End: 1615378

Strand: Reverse

Name: rmlA2 [C]

Synonym: XC_1328

Alternate gene names: 66767655

Gene position: 1615378-1614668 (Counterclockwise)

Preceding gene: 66767656

Following gene: 77761149

Centisome position: 31.37

GC content: 65.82

Gene sequence:

>711_bases
ATGAAGGCGTTGATCTTTGCTGCCGGATTCGGTGAGCGCATGCGTCCGTTGACCGAACACACGCCCAAGCCGCTGTTGTC
GGTGGGCGGCACGCCGTTGATCGTCTGGCATCTGCGCAAGTTGGCTGCACTGGGCGTGGACGAGGTGGTCATCAATACGT
CGTGGCTGGCTGCGCAGTTTCCGCAGGTGCTGGGCGATGGCAGCGCCTTCGGGCTGCGTCTGACCTATTCCTACGAAGGC
AGCACCCCGCTGGAAACCGGCGGCGGCATGCTGCACGCGCTGCCGTTGCTGGGCGATGCGCCCTTCCTGCTGGTCAATGG
CGACATCTGGAGCGACATCGACTTCGCCACGCTCGACATCGCAGCCGACAGCCTGGCAACGCTGGTGCTGGTCGACGCGC
CAGCCTATGCGGCACGCGCGGATTTTGCGTTGACTCACGAGGGCAAGATCAGCAGCACGGCGATGCCGACGCTCACCTAT
GCCGGCATCGGTCTGTATCGCCCGGCCTTACTGCACGCGTGGGAACGTGTGATCGGGCCGCTTCCCAGCACCGGTGAGGC
CCCGCCGCGCTTCCCGTTGGCGCCGATCCTGCGCGCGCAAATGGCGGCTGGCCGGATCGATGGCGTGCACCACCGTGGTC
GCTGGACCGATGTAGGCACACCACAGCGGCTGGCCGAGCTGGATGCACAACTGACTCAGCGCCGCGACTGA

Upstream 100 bases:

>100_bases
GCCATGCCGAATTGGCACCCTTGATCGACTTGCTCGACCAGCAGATCAAACCCGCACTGGCCGCACGCATGGCACACGGC
AACGCCAATGGAACCGCCGC

Downstream 100 bases:

>100_bases
GCGCGGCAGCGCCGTCGTCAGGACGTGCGGTCGCCGAGCACGCGGCGCAGGAACGGCACCGTCACCCGCCGCTGCGCAGC
CAGGGATTCACGGTCCAACC

Product: nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: Nucleotidyltransferase Family Protein; Sugar-Phosphate Nucleotidyl Transferase; Nucleoside-Diphosphate-Sugar Pyrophosphorylase; Nucleotidyl Transferase Family Protein; Mannose-1-Phosphate Guanylyltransferase; Nucleotidyltransferase; Nucleotidyl Transferase Superfamily; Nucleotide Phosphorylase; Mannose-1-Phosphate Guanyltransferase-Related Protein; Mannose-1-Phosphate Guanyltransferase; Transaldolase AB; ADP-Glucose Pyrophosphorylase; Mannose-1-Phosphate Guanyltransferase Protein; D-Alpha-D-Mannose-1-Phosphate Guanylyltransferase ManB; Aminoglycoside Phosphotransferase; Nucleotidyl Transferase Superfamily Protein; Nucleotidyl Transferase Protein; Sugar Phosphate Nucleotidyltransferase; Glucose-1-Phosphate Thymidylyltransferase; Sugar-Phosphate Nucleotide Transferase; Nucleotidyltransferase Protein

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKALIFAAGFGERMRPLTEHTPKPLLSVGGTPLIVWHLRKLAALGVDEVVINTSWLAAQFPQVLGDGSAFGLRLTYSYEG
STPLETGGGMLHALPLLGDAPFLLVNGDIWSDIDFATLDIAADSLATLVLVDAPAYAARADFALTHEGKISSTAMPTLTY
AGIGLYRPALLHAWERVIGPLPSTGEAPPRFPLAPILRAQMAAGRIDGVHHRGRWTDVGTPQRLAELDAQLTQRRD

Sequences:

>Translated_236_residues
MKALIFAAGFGERMRPLTEHTPKPLLSVGGTPLIVWHLRKLAALGVDEVVINTSWLAAQFPQVLGDGSAFGLRLTYSYEG
STPLETGGGMLHALPLLGDAPFLLVNGDIWSDIDFATLDIAADSLATLVLVDAPAYAARADFALTHEGKISSTAMPTLTY
AGIGLYRPALLHAWERVIGPLPSTGEAPPRFPLAPILRAQMAAGRIDGVHHRGRWTDVGTPQRLAELDAQLTQRRD
>Mature_236_residues
MKALIFAAGFGERMRPLTEHTPKPLLSVGGTPLIVWHLRKLAALGVDEVVINTSWLAAQFPQVLGDGSAFGLRLTYSYEG
STPLETGGGMLHALPLLGDAPFLLVNGDIWSDIDFATLDIAADSLATLVLVDAPAYAARADFALTHEGKISSTAMPTLTY
AGIGLYRPALLHAWERVIGPLPSTGEAPPRFPLAPILRAQMAAGRIDGVHHRGRWTDVGTPQRLAELDAQLTQRRD

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11761621, Length=235, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI11761619, Length=235, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=236, Percent_Identity=30.5084745762712, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320148, Length=121, Percent_Identity=33.0578512396694, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21355443, Length=233, Percent_Identity=26.1802575107296, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644084, Length=233, Percent_Identity=26.1802575107296, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 25319; Mature: 25319

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALIFAAGFGERMRPLTEHTPKPLLSVGGTPLIVWHLRKLAALGVDEVVINTSWLAAQF
CCEEEEECCCCCHHCCHHHCCCCCHHHCCCCEEHHHHHHHHHHCCCCCEEECCHHHHHHH
PQVLGDGSAFGLRLTYSYEGSTPLETGGGMLHALPLLGDAPFLLVNGDIWSDIDFATLDI
HHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEEHHCCCCCEEEECCCCCCCCCEEEEHH
AADSLATLVLVDAPAYAARADFALTHEGKISSTAMPTLTYAGIGLYRPALLHAWERVIGP
HHHHHEEEEEEECCCHHHHCCEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCC
LPSTGEAPPRFPLAPILRAQMAAGRIDGVHHRGRWTDVGTPQRLAELDAQLTQRRD
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKALIFAAGFGERMRPLTEHTPKPLLSVGGTPLIVWHLRKLAALGVDEVVINTSWLAAQF
CCEEEEECCCCCHHCCHHHCCCCCHHHCCCCEEHHHHHHHHHHCCCCCEEECCHHHHHHH
PQVLGDGSAFGLRLTYSYEGSTPLETGGGMLHALPLLGDAPFLLVNGDIWSDIDFATLDI
HHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEEHHCCCCCEEEECCCCCCCCCEEEEHH
AADSLATLVLVDAPAYAARADFALTHEGKISSTAMPTLTYAGIGLYRPALLHAWERVIGP
HHHHHEEEEEEECCCHHHHCCEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCC
LPSTGEAPPRFPLAPILRAQMAAGRIDGVHHRGRWTDVGTPQRLAELDAQLTQRRD
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA