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The map label for this gene is ptsN [H]

Identifier: 66767636

GI number: 66767636

Start: 1599762

End: 1600220

Strand: Reverse

Name: ptsN [H]

Synonym: XC_1309

Alternate gene names: 66767636

Gene position: 1600220-1599762 (Counterclockwise)

Preceding gene: 66767637

Following gene: 66767635

Centisome position: 31.08

GC content: 67.1

Gene sequence:

>459_bases
ATGCCTTTAACCGATCTGATGGCGGCCGTGCGCACCATGGTGTCGCCGGCCGCCGATCGCGACACCGTCCTGCATACCGC
CGCCGAGCTGCTGTCCTGCCGCCAGGCCGGTACCGACGAACTGTTTGCCAACCTGTGCGAGCGCGAAGCCCTTGGCAGCA
CCGCCATCGGCCACGGCATTGCCATTCCGCACGGGCGCTGCCCCAACCTCACCGAACCACGTGGCGCCTTGCTGCGGCTG
CAGACGCCGGTGGAGTTCGGCGCCGAAGAAGCCGTGGACCTGGTGTTTGCGATGGCGGTGCCGGCGCACTACACGCATCA
GCACCTGATGCTGTTGTCGGAACTGGCCGAGCGCTTTTCTGACAGCCATTTTCGCCAGCAGCTGCGCGCCGCGCCCGATG
CGCAAGCGCTGATGGCGCTGCTGACTGAGGTACCGCCTCCACACGCGAGCGCCGCATGA

Upstream 100 bases:

>100_bases
GCTCGACCGCCTGGTGCTCAAGCACAAAGAGAAGAAATGTAACCACCACGCCCGCGAGGTGCGCGACAATCCCGCATGAC
GCTTCTCGCTTGATGCCCTC

Downstream 100 bases:

>100_bases
ATACCAGCATCACCGCACGTGAACTGTTCGACCAGCAGCGCGACAAGCTGGCACTGCGCTGGGTTGCGGGGCAAAAGGGG
GAACACCGCGAGATCCAGGC

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MPLTDLMAAVRTMVSPAADRDTVLHTAAELLSCRQAGTDELFANLCEREALGSTAIGHGIAIPHGRCPNLTEPRGALLRL
QTPVEFGAEEAVDLVFAMAVPAHYTHQHLMLLSELAERFSDSHFRQQLRAAPDAQALMALLTEVPPPHASAA

Sequences:

>Translated_152_residues
MPLTDLMAAVRTMVSPAADRDTVLHTAAELLSCRQAGTDELFANLCEREALGSTAIGHGIAIPHGRCPNLTEPRGALLRL
QTPVEFGAEEAVDLVFAMAVPAHYTHQHLMLLSELAERFSDSHFRQQLRAAPDAQALMALLTEVPPPHASAA
>Mature_151_residues
PLTDLMAAVRTMVSPAADRDTVLHTAAELLSCRQAGTDELFANLCEREALGSTAIGHGIAIPHGRCPNLTEPRGALLRLQ
TPVEFGAEEAVDLVFAMAVPAHYTHQHLMLLSELAERFSDSHFRQQLRAAPDAQALMALLTEVPPPHASAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=106, Percent_Identity=41.5094339622642, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1788726, Length=103, Percent_Identity=33.0097087378641, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1786951, Length=140, Percent_Identity=29.2857142857143, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI48994992, Length=133, Percent_Identity=32.3308270676692, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16341; Mature: 16210

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDLMAAVRTMVSPAADRDTVLHTAAELLSCRQAGTDELFANLCEREALGSTAIGHGI
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCCC
AIPHGRCPNLTEPRGALLRLQTPVEFGAEEAVDLVFAMAVPAHYTHQHLMLLSELAERFS
CCCCCCCCCCCCCCCCEEEECCCHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
DSHFRQQLRAAPDAQALMALLTEVPPPHASAA
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PLTDLMAAVRTMVSPAADRDTVLHTAAELLSCRQAGTDELFANLCEREALGSTAIGHGI
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCCC
AIPHGRCPNLTEPRGALLRLQTPVEFGAEEAVDLVFAMAVPAHYTHQHLMLLSELAERFS
CCCCCCCCCCCCCCCCEEEECCCHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
DSHFRQQLRAAPDAQALMALLTEVPPPHASAA
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]