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The map label for this gene is garL [H]

Identifier: 66767437

GI number: 66767437

Start: 1339192

End: 1339953

Strand: Reverse

Name: garL [H]

Synonym: XC_1109

Alternate gene names: 66767437

Gene position: 1339953-1339192 (Counterclockwise)

Preceding gene: 66767438

Following gene: 66767436

Centisome position: 26.03

GC content: 67.72

Gene sequence:

>762_bases
ATGTCACTGCGTGTTGGCGTTCTGAATGCACTGCCGAGCGTGCAGCTGTGCGAACTGCTGGGCCGGATCGGCTACGGGTT
TGTGGTGCTGGACCTGGAACATATGCTGCGCGCGCCGGACGAACTGGAACACGCCATCCGCGCCTGCGAGCTGTCCGGCT
GCGAGGCCTGGGTGCGGGTGCCGGAGGTGGACGAGAAACTGATCGGCCGCGTGCTGGACGCCGGCGCGCGTGGCATCGTG
GTTCCGCGCGCCGAATCGGCTGCGCAGATCGCTAGTGCGATTGCCGCGGCACGCTTTCCGCCGCTGGGCCGGCGCGGCAT
CACCGGGGGCCGCGTCACCGGCTTCGGCAACGTCGATCTGCCCACCTATATCGCGCAGGCCAACCGCGACATCCGCATTG
TGCCGATGATCGAAAGCGCGGCCGGCATTGCGGCGTTGCCGCAGATTCTGGCCGTGCCCGGCGTGACCCTGGTGATGGAA
GGCGCGCTCGATCTTGCGCTGGATCTGGGCCTTGGGCCACAGCCCACGCATCCACAGGTGTGGGCCTTGCTGCTGCAAAT
GGACGCGCAATGCCGCAGCGCTGCGGTTCCGTTCTGCCCCAACCCGCGCACTGACGCGCAACGCGCACACTGGTTGCAGC
AACCGGATCCGCGCTGGCTGCTTGCCGGCGAAGACCGTGCCCTGATCCAACGCGCATTGCGCGGCCAGTTGGCCACCTTT
GCCGCACCCCTTTCATCACCCGCCTGCAGGAGCACGCCGTAG

Upstream 100 bases:

>100_bases
GAAAATGCGACTCGCGATCCAGAAAAACACCCTTCAACACACTTGATAATCATTCCCATCAAGCGCAGGATGAGCGCCCG
TTCCCCTGGCGGCCTCATCC

Downstream 100 bases:

>100_bases
ATGCCGACGTTCCGTTTCCACGCCCTGGCCATTGCCATCGCCGCCACCACCTTGAGCGCTGCACCGCTGGCGCGGGCCGG
CGAGACCGACGGCACTGCGG

Product: 4-hydroxy-2-oxovalerate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSLRVGVLNALPSVQLCELLGRIGYGFVVLDLEHMLRAPDELEHAIRACELSGCEAWVRVPEVDEKLIGRVLDAGARGIV
VPRAESAAQIASAIAAARFPPLGRRGITGGRVTGFGNVDLPTYIAQANRDIRIVPMIESAAGIAALPQILAVPGVTLVME
GALDLALDLGLGPQPTHPQVWALLLQMDAQCRSAAVPFCPNPRTDAQRAHWLQQPDPRWLLAGEDRALIQRALRGQLATF
AAPLSSPACRSTP

Sequences:

>Translated_253_residues
MSLRVGVLNALPSVQLCELLGRIGYGFVVLDLEHMLRAPDELEHAIRACELSGCEAWVRVPEVDEKLIGRVLDAGARGIV
VPRAESAAQIASAIAAARFPPLGRRGITGGRVTGFGNVDLPTYIAQANRDIRIVPMIESAAGIAALPQILAVPGVTLVME
GALDLALDLGLGPQPTHPQVWALLLQMDAQCRSAAVPFCPNPRTDAQRAHWLQQPDPRWLLAGEDRALIQRALRGQLATF
AAPLSSPACRSTP
>Mature_252_residues
SLRVGVLNALPSVQLCELLGRIGYGFVVLDLEHMLRAPDELEHAIRACELSGCEAWVRVPEVDEKLIGRVLDAGARGIVV
PRAESAAQIASAIAAARFPPLGRRGITGGRVTGFGNVDLPTYIAQANRDIRIVPMIESAAGIAALPQILAVPGVTLVMEG
ALDLALDLGLGPQPTHPQVWALLLQMDAQCRSAAVPFCPNPRTDAQRAHWLQQPDPRWLLAGEDRALIQRALRGQLATFA
APLSSPACRSTP

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=182, Percent_Identity=34.6153846153846, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788578, Length=255, Percent_Identity=26.2745098039216, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27015; Mature: 26884

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRVGVLNALPSVQLCELLGRIGYGFVVLDLEHMLRAPDELEHAIRACELSGCEAWVRV
CCCCCCHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHCCCCHHEEEC
PEVDEKLIGRVLDAGARGIVVPRAESAAQIASAIAAARFPPLGRRGITGGRVTGFGNVDL
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCH
PTYIAQANRDIRIVPMIESAAGIAALPQILAVPGVTLVMEGALDLALDLGLGPQPTHPQV
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHEEHHHHHHHHEECCCCCCCCCHHH
WALLLQMDAQCRSAAVPFCPNPRTDAQRAHWLQQPDPRWLLAGEDRALIQRALRGQLATF
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
AAPLSSPACRSTP
HHCCCCCCCCCCC
>Mature Secondary Structure 
SLRVGVLNALPSVQLCELLGRIGYGFVVLDLEHMLRAPDELEHAIRACELSGCEAWVRV
CCCCCHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHCCCCHHEEEC
PEVDEKLIGRVLDAGARGIVVPRAESAAQIASAIAAARFPPLGRRGITGGRVTGFGNVDL
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCH
PTYIAQANRDIRIVPMIESAAGIAALPQILAVPGVTLVMEGALDLALDLGLGPQPTHPQV
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHEEHHHHHHHHEECCCCCCCCCHHH
WALLLQMDAQCRSAAVPFCPNPRTDAQRAHWLQQPDPRWLLAGEDRALIQRALRGQLATF
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
AAPLSSPACRSTP
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA