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The map label for this gene is otsB [H]

Identifier: 66767406

GI number: 66767406

Start: 1299710

End: 1300468

Strand: Reverse

Name: otsB [H]

Synonym: XC_1078

Alternate gene names: 66767406

Gene position: 1300468-1299710 (Counterclockwise)

Preceding gene: 66767407

Following gene: 66767405

Centisome position: 25.26

GC content: 67.59

Gene sequence:

>759_bases
ATGGCAGACTTGCATTCCCCCCTTCCGTCGCCGCCATTGCTGGACGATGCCTGCGCATTGTTCCTGGACGTGGACGGCAC
CCTGATCGACTTTGCGCACACCCCCGAAGCAGTGCAGTTGCTGCCGGAGGTACGCGACGCAATCGCGCGCCTTAGCGAGC
GCCTTGGCGGCGCCGTCGCCCTGGTCAGCGGGCGGCCGCTATCCCAACTGGACGCCCTGTTCGCACCCCTGCTGCTGCCG
GCCGCCGGCCTGCACGGGCACGAGTTGCGCAGCGACGCAGCCGCACGCGCGGCAATGCCGCAAGACACCTCCGAATGGTT
GCACGGCTTGCATAAACGCGCCGCTGCACTCACGCATCAACATCCTGGCGTGCTGGTCGAAGACAAAGGCGTCAGCGTCG
CTCTGCATTGGCGCGCGCAACCACTTGCCGGCCCCGACGTACTCGCCTTCGCACAGCAGGAAATCGCGGCGCTGTCGGGT
TACCGCCTACAGCCCGGCGATCACGTCGTTGAATTTGTTCCCGAAGGCAGCAACAAGGGCCTGGCGGTCGAGCAGCTGAT
GCAGCAGCCGGCATTTGCGGGCCGCACGCCGGTGTTTGTCGGCGACGACCTCACCGACGAATTCGGTTTCCAGGCGGCCA
ATCGCCTCGGCGGCTGGAGCGTGCTGGTCGGCGACCGCGCCCAGACCGACGCACGCTTCCGCGTGTCGGGCACCGCCGCC
GTGCATGCCTGGTTGCAGCGCAGCGCGCACACCGCGTGA

Upstream 100 bases:

>100_bases
CCTCATCCGCCCCTGCGGGGCACCTTCTCCCGACGGGAGAAGGGATGGCAGTCGCCGTTGCATAACCTGAATATCACATC
GCTTTGCGCACACTAGCGCG

Downstream 100 bases:

>100_bases
GCGCGTTACGCGCTCCCCATCGTTTTTCCCCCAAGACGTTACGCATTTCATGACCGAGTCTTCTCTGGATCTGGGCGTCA
TCGGCAATTGCAGTTTTGGT

Product: trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MADLHSPLPSPPLLDDACALFLDVDGTLIDFAHTPEAVQLLPEVRDAIARLSERLGGAVALVSGRPLSQLDALFAPLLLP
AAGLHGHELRSDAAARAAMPQDTSEWLHGLHKRAAALTHQHPGVLVEDKGVSVALHWRAQPLAGPDVLAFAQQEIAALSG
YRLQPGDHVVEFVPEGSNKGLAVEQLMQQPAFAGRTPVFVGDDLTDEFGFQAANRLGGWSVLVGDRAQTDARFRVSGTAA
VHAWLQRSAHTA

Sequences:

>Translated_252_residues
MADLHSPLPSPPLLDDACALFLDVDGTLIDFAHTPEAVQLLPEVRDAIARLSERLGGAVALVSGRPLSQLDALFAPLLLP
AAGLHGHELRSDAAARAAMPQDTSEWLHGLHKRAAALTHQHPGVLVEDKGVSVALHWRAQPLAGPDVLAFAQQEIAALSG
YRLQPGDHVVEFVPEGSNKGLAVEQLMQQPAFAGRTPVFVGDDLTDEFGFQAANRLGGWSVLVGDRAQTDARFRVSGTAA
VHAWLQRSAHTA
>Mature_251_residues
ADLHSPLPSPPLLDDACALFLDVDGTLIDFAHTPEAVQLLPEVRDAIARLSERLGGAVALVSGRPLSQLDALFAPLLLPA
AGLHGHELRSDAAARAAMPQDTSEWLHGLHKRAAALTHQHPGVLVEDKGVSVALHWRAQPLAGPDVLAFAQQEIAALSGY
RLQPGDHVVEFVPEGSNKGLAVEQLMQQPAFAGRTPVFVGDDLTDEFGFQAANRLGGWSVLVGDRAQTDARFRVSGTAAV
HAWLQRSAHTA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=250, Percent_Identity=38.8, Blast_Score=152, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221473368, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129307, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582490, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26799; Mature: 26667

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLHSPLPSPPLLDDACALFLDVDGTLIDFAHTPEAVQLLPEVRDAIARLSERLGGAVA
CCCCCCCCCCCCCHHHHHEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
LVSGRPLSQLDALFAPLLLPAAGLHGHELRSDAAARAAMPQDTSEWLHGLHKRAAALTHQ
EECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
HPGVLVEDKGVSVALHWRAQPLAGPDVLAFAQQEIAALSGYRLQPGDHVVEFVPEGSNKG
CCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
LAVEQLMQQPAFAGRTPVFVGDDLTDEFGFQAANRLGGWSVLVGDRAQTDARFRVSGTAA
HHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCHHH
VHAWLQRSAHTA
HHHHHHHHCCCC
>Mature Secondary Structure 
ADLHSPLPSPPLLDDACALFLDVDGTLIDFAHTPEAVQLLPEVRDAIARLSERLGGAVA
CCCCCCCCCCCCHHHHHEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
LVSGRPLSQLDALFAPLLLPAAGLHGHELRSDAAARAAMPQDTSEWLHGLHKRAAALTHQ
EECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
HPGVLVEDKGVSVALHWRAQPLAGPDVLAFAQQEIAALSGYRLQPGDHVVEFVPEGSNKG
CCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
LAVEQLMQQPAFAGRTPVFVGDDLTDEFGFQAANRLGGWSVLVGDRAQTDARFRVSGTAA
HHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCHHH
VHAWLQRSAHTA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]