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The map label for this gene is xpsO

Identifier: 66767384

GI number: 66767384

Start: 1271594

End: 1272457

Strand: Reverse

Name: xpsO

Synonym: XC_1056

Alternate gene names: 66767384

Gene position: 1272457-1271594 (Counterclockwise)

Preceding gene: 77761128

Following gene: 66767383

Centisome position: 24.71

GC content: 64.35

Gene sequence:

>864_bases
ATGGCATTTCTCGACCAGCATCCCGGTCTCGGCTTTCCCGCCGCGGCCGGACTGGGACTGCTGATCGGCAGCTTCCTGAA
TGTAGTGATCCTGCGCTTGCCCAAGCGCATGGAGTGGCAGTGGCGGCGCGATGCGCGCGAAATCCTGGAACTGCCCGACA
TCTACGAGCCGCCGCCGCCGGGCATTGTGGTGGAGCCATCGCACGATCCGGTCACCGGCGACAAGCTCAAGTGGTGGGAG
AACATTCCGCTGTTCAGCTGGCTGATGCTGCGCGGCAAGTCGCGCTACAGCGGCAAGCCGATCTCCATCCAGTACCCGCT
GGTGGAGCTGTTGACCTCGATCCTGTGCGTGGCCAGCGTCTGGCGCTTCGGCTTCGGCTGGCAGGGCTTCGGTGCGATCG
TGCTGAGCTGCTTTCTGGTGGCGATGTCGGGTATCGACCTGCGCCACAAGCTGCTGCCGGACCAGCTGACCTTGCCGCTG
ATGTGGTTGGGCTTGGTCGGTTCGATGGACAACCTCTACATGCCAGCCAAGCCCGCCCTGCTGGGTGCGGCGGTCGGCTA
TGTCTCGCTCTGGACGGTGTGGTGGCTGTTCAAGCAGCTCACCGGCAAGGAAGGGATGGGCCACGGCGACTTCAAGTTGC
TGGCTGCGCTGGGGGCGTGGTGCGGGTTGAAGGGCATTCTGCCGATCATCCTGATCTCCTCGCTGGTCGGCGCCGTGCTC
GGTTCGATCTGGCTGTTCGCCAAGGGGCGCGACCGCGCCACGCCGATCCCGTTCGGACCTTATCTGGCCATCGCCGGCTG
GGTAGTGTTCTTCTGGGGTAACGACCTGGTGGATGGCTACCTGCGTTTCGCAGGCCTGCGTTGA

Upstream 100 bases:

>100_bases
GGAACCATTGATCATGGTTTTTATTGGCACAATTGTGGGCGGCATGGTCATTGGCATGTATCTTCCCATCTTCAAACTTG
GCTCAGTGGTCTAAAACGTA

Downstream 100 bases:

>100_bases
GCAAGCCACGACGATGAGCAACTTCATCGTCGGCCTCACCGGCGGCATCGCCTCCGGCAAAAGCGCACTTGCCGCTGAGT
TCGAGAAGTTGGGCGTGCCG

Product: type IV pre-pilin leader peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPPGIVVEPSHDPVTGDKLKWWE
NIPLFSWLMLRGKSRYSGKPISIQYPLVELLTSILCVASVWRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPL
MWLGLVGSMDNLYMPAKPALLGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAVL
GSIWLFAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLRFAGLR

Sequences:

>Translated_287_residues
MAFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPPGIVVEPSHDPVTGDKLKWWE
NIPLFSWLMLRGKSRYSGKPISIQYPLVELLTSILCVASVWRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPL
MWLGLVGSMDNLYMPAKPALLGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAVL
GSIWLFAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLRFAGLR
>Mature_286_residues
AFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPPGIVVEPSHDPVTGDKLKWWEN
IPLFSWLMLRGKSRYSGKPISIQYPLVELLTSILCVASVWRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPLM
WLGLVGSMDNLYMPAKPALLGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAVLG
SIWLFAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLRFAGLR

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family

Homologues:

Organism=Escherichia coli, GI87082194, Length=256, Percent_Identity=34.765625, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789732, Length=128, Percent_Identity=46.875, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP4_XANCP (Q56763)

Other databases:

- EMBL:   U12432
- EMBL:   AE008922
- PIR:   S70810
- RefSeq:   NP_638448.1
- MEROPS:   A24.001
- GeneID:   998351
- GenomeReviews:   AE008922_GR
- KEGG:   xcc:XCC3101
- HOGENOM:   HBG710101
- OMA:   VFWLFKL
- ProtClustDB:   CLSK446534
- BioCyc:   XCAM190485:XCC3101-MONOMER
- BRENDA:   3.4.23.43
- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045
- PRINTS:   PR00864

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24

EC number: =3.4.23.43

Molecular weight: Translated: 31843; Mature: 31712

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x138af148)-; HASH(0x141cd998)-; HASH(0x1373e42c)-; HASH(0x1456cfbc)-; HASH(0x13f018c8)-; HASH(0x11f677c0)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GIVVEPSHDPVTGDKLKWWENIPLFSWLMLRGKSRYSGKPISIQYPLVELLTSILCVASV
CEEECCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
WRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPLMWLGLVGSMDNLYMPAKPAL
HHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
LGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSIWLFAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLRFAGLR
HHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCH
>Mature Secondary Structure 
AFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GIVVEPSHDPVTGDKLKWWENIPLFSWLMLRGKSRYSGKPISIQYPLVELLTSILCVASV
CEEECCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
WRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPLMWLGLVGSMDNLYMPAKPAL
HHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
LGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSIWLFAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLRFAGLR
HHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8817497; 12024217