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The map label for this gene is mscL [H]

Identifier: 66767341

GI number: 66767341

Start: 1222188

End: 1222619

Strand: Reverse

Name: mscL [H]

Synonym: XC_1009

Alternate gene names: 66767341

Gene position: 1222619-1222188 (Counterclockwise)

Preceding gene: 66767342

Following gene: 66767340

Centisome position: 23.75

GC content: 59.95

Gene sequence:

>432_bases
ATGGGAATGGTCAGCGAATTCAAGCAATTCGCGATGCGTGGCAATGTCATCGACCTGGCGGTCGGTGTGGTCATCGGCGC
GGCGTTCGGCAAGATCGTCACCGCGCTGGTGGAAAAGATCATCATGCCGCCGATCGGCTGGGCCATCGGCAACGTGGATT
TTTCGCGGCTGGCGTGGGTGCTCAAACCGGCGGGCGTGGATGCCACCGGCAAGGAAATTCCTGCCGTGGTGATCGGCTAC
GGTGACTTCATCAACACCGTCGTGCAGTTCGTGATCATTGCCTTTGCGATCTTCCTGGTGGTCAAACTGATCAACCGCCT
CAGCCAGCGCAAGCCGGATGCTCCCAAGGGCCCGAGCGAAGAAGTGCTGCTGCTGCGCGAGATCCGCGACTCGCTCAAGA
ACGACACGCTCAAGAATCCGACCGTGCCGTAA

Upstream 100 bases:

>100_bases
AAAACGTGGCCGAACGCCACGGCATCATCGCGGACTGACATCGGCCGGGCTACGATCGCTGCAGCGGCAGTGGGTCCGCG
TCCCCCATCTGGAGTGCACT

Downstream 100 bases:

>100_bases
ACAAGCGGCCACATCCTTGCGCGCACTTCGCACACGAGGATGACTTTTCGCACGCAGACGGGGGACCTCGCGCTGATAAG
CTCGGAGTCCCCCTTGTCGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MGMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWVLKPAGVDATGKEIPAVVIGY
GDFINTVVQFVIIAFAIFLVVKLINRLSQRKPDAPKGPSEEVLLLREIRDSLKNDTLKNPTVP

Sequences:

>Translated_143_residues
MGMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWVLKPAGVDATGKEIPAVVIGY
GDFINTVVQFVIIAFAIFLVVKLINRLSQRKPDAPKGPSEEVLLLREIRDSLKNDTLKNPTVP
>Mature_142_residues
GMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWVLKPAGVDATGKEIPAVVIGYG
DFINTVVQFVIIAFAIFLVVKLINRLSQRKPDAPKGPSEEVLLLREIRDSLKNDTLKNPTVP

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=138, Percent_Identity=55.7971014492754, Blast_Score=146, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15487; Mature: 15355

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWV
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHH
LKPAGVDATGKEIPAVVIGYGDFINTVVQFVIIAFAIFLVVKLINRLSQRKPDAPKGPSE
HCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EVLLLREIRDSLKNDTLKNPTVP
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWV
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHH
LKPAGVDATGKEIPAVVIGYGDFINTVVQFVIIAFAIFLVVKLINRLSQRKPDAPKGPSE
HCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EVLLLREIRDSLKNDTLKNPTVP
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]