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The map label for this gene is rfbB [C]

Identifier: 66048330

GI number: 66048330

Start: 6058111

End: 6059106

Strand: Direct

Name: rfbB [C]

Synonym: Psyr_5106

Alternate gene names: 66048330

Gene position: 6058111-6059106 (Clockwise)

Preceding gene: 66048329

Following gene: 66048331

Centisome position: 99.42

GC content: 61.45

Gene sequence:

>996_bases
ATGACGGTACTGGTGACAGGTGCTGCAGGGTTCATCGGGTTTCATGTCGCCAAGCGTCTGTGCGAGCTCGGCGTAGAGGT
CGTCGGCATCGACAACCTCAACGATTACTACAGCGTCGAGCTCAAACAATCGCGCCTGGCCCTACTCCAGCGCCTGCCAG
GTTTCACGTTCCACAGGCTGGACATCACCGATGCCGAAGGGCTGTCCGCGCTCTTCGCGCAAAATGGCTTCGAACAGGTC
ATTCATCTGGCGGCTCAGGCCGGTGTGCGTTATTCGCTTGAACAACCGAATGTTTACGCCCAATCCAATCTGGTCGGCTT
TATCAACGTGCTCGAGGCCTGTCGGCAGTATCGACCGGCGCACCTGATCTACGCGTCGAGCAGCTCGGTCTACGGCGCCA
ATACGCGCATGCCGTTTCAGGTCGAGGATGCGGTCGACCGGCCGCTGTCCCTGTATGCCGCCACCAAGCGCGCCAACGAA
CTCACCGCCTACAGCTATTGCCACTTGTACGGCCTGCGCGCCACAGGGCTGCGCTTTTTCACCGTCTACGGCCCCTGGGG
CCGGCCGGACATGGCGCTGTTCAAGTTCACCAAAGCCATGCTGGCCGGCCAGCCTGTGGATATCTACAACCACGGCGAAA
TGGCGCGGGACTTCACCTACATCGACGACATTGTCGAAAGCATCCTGCGCCTGCGTCTGCTGCCGCCCGATGCGGTTGGC
AGCGAGCCGCCCCATCAACTCTTCAACATCGGACGGGGTCAGCCCGTCAAGCTGCTGGAGTTCGTCGATTGCCTGGAGGC
CGCGCTAGGCCTGCGCGCGGAACGGCGCTACCTGCCCTTGCAAGCGGGCGACGTGCTGCAAACCTGGGCTGACGTCAGTG
CGCTGAGCCAATGGATCGACTTTCAACCACAGGTCTCGGTCGACACCGGCGTCAGGGCCTTCGTCGACTGGTATCGGGAG
CACTATCAGGCCCGGATCTGCGAGCCACTTCAATAA

Upstream 100 bases:

>100_bases
ATCGCGTGGTGTTCGATGGCCGCAACCTGTTCGACCCGTTGCAGCTGCGTGAGGCGGGCTTTACCTACTACGGCATTGGT
CGTGGCCAGGCGACGCCGGC

Downstream 100 bases:

>100_bases
CAAGGACTTCCATGACCACGCCCACTTTTCTCTCCGTCGTGATCCCGGCCAAAAACGAAGCCGATAACCTGCCCAGCCTG
ATAAAAGAAATTTGCACAGC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTVLVTGAAGFIGFHVAKRLCELGVEVVGIDNLNDYYSVELKQSRLALLQRLPGFTFHRLDITDAEGLSALFAQNGFEQV
IHLAAQAGVRYSLEQPNVYAQSNLVGFINVLEACRQYRPAHLIYASSSSVYGANTRMPFQVEDAVDRPLSLYAATKRANE
LTAYSYCHLYGLRATGLRFFTVYGPWGRPDMALFKFTKAMLAGQPVDIYNHGEMARDFTYIDDIVESILRLRLLPPDAVG
SEPPHQLFNIGRGQPVKLLEFVDCLEAALGLRAERRYLPLQAGDVLQTWADVSALSQWIDFQPQVSVDTGVRAFVDWYRE
HYQARICEPLQ

Sequences:

>Translated_331_residues
MTVLVTGAAGFIGFHVAKRLCELGVEVVGIDNLNDYYSVELKQSRLALLQRLPGFTFHRLDITDAEGLSALFAQNGFEQV
IHLAAQAGVRYSLEQPNVYAQSNLVGFINVLEACRQYRPAHLIYASSSSVYGANTRMPFQVEDAVDRPLSLYAATKRANE
LTAYSYCHLYGLRATGLRFFTVYGPWGRPDMALFKFTKAMLAGQPVDIYNHGEMARDFTYIDDIVESILRLRLLPPDAVG
SEPPHQLFNIGRGQPVKLLEFVDCLEAALGLRAERRYLPLQAGDVLQTWADVSALSQWIDFQPQVSVDTGVRAFVDWYRE
HYQARICEPLQ
>Mature_330_residues
TVLVTGAAGFIGFHVAKRLCELGVEVVGIDNLNDYYSVELKQSRLALLQRLPGFTFHRLDITDAEGLSALFAQNGFEQVI
HLAAQAGVRYSLEQPNVYAQSNLVGFINVLEACRQYRPAHLIYASSSSVYGANTRMPFQVEDAVDRPLSLYAATKRANEL
TAYSYCHLYGLRATGLRFFTVYGPWGRPDMALFKFTKAMLAGQPVDIYNHGEMARDFTYIDDIVESILRLRLLPPDAVGS
EPPHQLFNIGRGQPVKLLEFVDCLEAALGLRAERRYLPLQAGDVLQTWADVSALSQWIDFQPQVSVDTGVRAFVDWYREH
YQARICEPLQ

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=25.5192878338279, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=343, Percent_Identity=25.9475218658892, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI56118217, Length=343, Percent_Identity=25.9475218658892, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI189083684, Length=343, Percent_Identity=25.9475218658892, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=25.1497005988024, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=26.0869565217391, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=26.2536873156342, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=24.6376811594203, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=25.2808988764045, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=25.4237288135593, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=328, Percent_Identity=26.219512195122, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=243, Percent_Identity=27.5720164609054, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=347, Percent_Identity=22.7665706051873, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=357, Percent_Identity=27.7310924369748, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19923002, Length=332, Percent_Identity=27.1084337349398, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=24.4648318042813, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37176; Mature: 37045

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVTGAAGFIGFHVAKRLCELGVEVVGIDNLNDYYSVELKQSRLALLQRLPGFTFHRL
CEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHCCCCEEEEE
DITDAEGLSALFAQNGFEQVIHLAAQAGVRYSLEQPNVYAQSNLVGFINVLEACRQYRPA
ECCCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHHHHHHHCCCE
HLIYASSSSVYGANTRMPFQVEDAVDRPLSLYAATKRANELTAYSYCHLYGLRATGLRFF
EEEEECCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHCHHHHHHHHHHEEEEECCEEEE
TVYGPWGRPDMALFKFTKAMLAGQPVDIYNHGEMARDFTYIDDIVESILRLRLLPPDAVG
EEECCCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
SEPPHQLFNIGRGQPVKLLEFVDCLEAALGLRAERRYLPLQAGDVLQTWADVSALSQWID
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHC
FQPQVSVDTGVRAFVDWYREHYQARICEPLQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVLVTGAAGFIGFHVAKRLCELGVEVVGIDNLNDYYSVELKQSRLALLQRLPGFTFHRL
EEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEHHHHHHHHHHCCCCEEEEE
DITDAEGLSALFAQNGFEQVIHLAAQAGVRYSLEQPNVYAQSNLVGFINVLEACRQYRPA
ECCCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHHHHHHHCCCE
HLIYASSSSVYGANTRMPFQVEDAVDRPLSLYAATKRANELTAYSYCHLYGLRATGLRFF
EEEEECCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHCHHHHHHHHHHEEEEECCEEEE
TVYGPWGRPDMALFKFTKAMLAGQPVDIYNHGEMARDFTYIDDIVESILRLRLLPPDAVG
EEECCCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
SEPPHQLFNIGRGQPVKLLEFVDCLEAALGLRAERRYLPLQAGDVLQTWADVSALSQWID
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHC
FQPQVSVDTGVRAFVDWYREHYQARICEPLQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]