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The map label for this gene is puuA [H]

Identifier: 66048092

GI number: 66048092

Start: 5778347

End: 5779723

Strand: Direct

Name: puuA [H]

Synonym: Psyr_4868

Alternate gene names: 66048092

Gene position: 5778347-5779723 (Clockwise)

Preceding gene: 66048084

Following gene: 66048093

Centisome position: 94.82

GC content: 58.61

Gene sequence:

>1377_bases
ATGTCGGTACCCCCGCGTGCCGTTCAGCTTAACGAAGCGAACGCGTTCCTTAAGGAACATCCTGAGGTTCTGTACGTTGA
CCTTCTGATTGCAGATATGAATGGTGTGGTGCGTGGCAAACGCATCGAACGCACCAGCCTCCATAAGGTTTACGAGAAGG
GCATCAACCTGCCTGCCTCTTTATTTGCTCTGGATATCAATGGCTCCACGGTGGAGAGCACCGGTCTGGGCCTGGACATC
GGTGATGCTGACCGAATCTGTTATCCCATCCCCGACACCCTGTGCAACGAGCCATGGCAAAAGCGCCCTACCGCGCAATT
GCTGATGACCATGCACGAACTCGAAGGCGATCCGTTTTTCGCCGACCCGCGTGAAGTATTGCGCCAGGTCGTCGCCAAAT
TCGACGAAATGGGCCTGACCATCTGCGCCGCGTTCGAGCTGGAGTTCTACCTGATCGATCAGGAGAACGTGAACGGTCGC
CCACAACCGCCCCGCTCGCCGATCTCCGGCAAACGCCCGCATTCGACACAGGTCTACCTGATCGACGACCTCGACGAATA
CGTCGACTGCCTTCAGGACATCCTCGAAGGTGCGAAGGAACAAGGCATTCCTGCCGACGCCATCGTCAAGGAAAGCGCCC
CGGCCCAGTTCGAAGTCAACCTGCATCACGTCGCCGACGCGATCAAGGCGTGCGATTACGCGGTATTGCTCAAGCGCCTG
ATCAAGAACATCGCCTACGATCACGAGATGGACACGACCTTCATGGCCAAGCCCTATCCGGGTCAGGCGGGTAATGGTCT
GCACGTTCATATTTCGATTCTGGATCGCGACGGCAAGAACATCTTCACCAGCGAGGATCCCGAGCAGAACGCCGCATTGC
GTCACGCAATCGGCGGTGTGCTCGAGACCCTGCCGGCACAAATGGCCTTCCTGTGCCCGAACGTCAACTCGTACCGCCGG
TTCGGTGCGCAGTTCTATGTGCCCAACTCACCGACCTGGGGCCTGGATAACCGTACGGTCGCCGTTCGCGTACCGACCGG
CACCGCCGATGCGGTACGTATCGAACACCGCGTTGCCGGCGCCGACGCCAACCCGTATCTGGTGATGGCTTCGGTCCTGG
CGGGCGTGCACCACGGCCTGGTCAACAAGGTCGAGCCTGGCGCTCCGGTCGAAGGCAACTCCTACGAGCAACACGAACAA
AGCCTGCCGAACAACCTGCGCGACGCATTGCGCGAGCTGGACGACAACCCGGTGATGGCCAAGTACATCGACCCGAAATA
CATCGACATTTTTGTCGCCTGTAAAGAGAGCGAGCTGGAAGAGTTCGAACACTCCATCTCCGACCTCGAGTACAACTGGT
ACCTGCATACGGTCTGA

Upstream 100 bases:

>100_bases
AGGGCAAAAAAAGGCTCTATTGACTTCATCATGGCTTTCGGATTGACTGAGATCCGCAATCATTGATGATTGATATTTTT
AACAACAAAGGTGTTGCATC

Downstream 100 bases:

>100_bases
TTGAGGCCGAGTAACACAAAACGCCGCAGGCTTTGAGCCTTGCGGCGTTTTGGTTTGGCGTGCATGCCCTCCCCCGGACA
CCTGTCGTTCCTCACGCTCC

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDI
GDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDEMGLTICAAFELEFYLIDQENVNGR
PQPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADAIKACDYAVLLKRL
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDRDGKNIFTSEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRR
FGAQFYVPNSPTWGLDNRTVAVRVPTGTADAVRIEHRVAGADANPYLVMASVLAGVHHGLVNKVEPGAPVEGNSYEQHEQ
SLPNNLRDALRELDDNPVMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV

Sequences:

>Translated_458_residues
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDI
GDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDEMGLTICAAFELEFYLIDQENVNGR
PQPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADAIKACDYAVLLKRL
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDRDGKNIFTSEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRR
FGAQFYVPNSPTWGLDNRTVAVRVPTGTADAVRIEHRVAGADANPYLVMASVLAGVHHGLVNKVEPGAPVEGNSYEQHEQ
SLPNNLRDALRELDDNPVMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV
>Mature_457_residues
SVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDIG
DADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDEMGLTICAAFELEFYLIDQENVNGRP
QPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADAIKACDYAVLLKRLI
KNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDRDGKNIFTSEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRRF
GAQFYVPNSPTWGLDNRTVAVRVPTGTADAVRIEHRVAGADANPYLVMASVLAGVHHGLVNKVEPGAPVEGNSYEQHEQS
LPNNLRDALRELDDNPVMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=429, Percent_Identity=23.0769230769231, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI87081870, Length=445, Percent_Identity=43.8202247191011, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1790301, Length=480, Percent_Identity=24.1666666666667, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51035; Mature: 50904

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
CCCCCCEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LFALDINGSTVESTGLGLDIGDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFF
EEEEECCCCEEECCCCCEECCCCCCEEECCCHHHCCCCHHCCCHHHHHHHHHHHCCCCCC
ADPREVLRQVVAKFDEMGLTICAAFELEFYLIDQENVNGRPQPPRSPISGKRPHSTQVYL
CCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEE
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADAIKACDYAVLLKRL
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHCCHHHHHHHH
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDRDGKNIFTSEDPEQNAALRHAIGGV
HHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPTWGLDNRTVAVRVPTGTADAVRIEHRVAG
HHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEHEECC
ADANPYLVMASVLAGVHHGLVNKVEPGAPVEGNSYEQHEQSLPNNLRDALRELDDNPVMA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCEEE
KYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV
EECCCCEEEEEEEECHHHHHHHHHHHHCCEEEEEEEEC
>Mature Secondary Structure 
SVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
CCCCCEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LFALDINGSTVESTGLGLDIGDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFF
EEEEECCCCEEECCCCCEECCCCCCEEECCCHHHCCCCHHCCCHHHHHHHHHHHCCCCCC
ADPREVLRQVVAKFDEMGLTICAAFELEFYLIDQENVNGRPQPPRSPISGKRPHSTQVYL
CCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEE
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADAIKACDYAVLLKRL
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHCCHHHHHHHH
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDRDGKNIFTSEDPEQNAALRHAIGGV
HHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPTWGLDNRTVAVRVPTGTADAVRIEHRVAG
HHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEHEECC
ADANPYLVMASVLAGVHHGLVNKVEPGAPVEGNSYEQHEQSLPNNLRDALRELDDNPVMA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCEEE
KYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV
EECCCCEEEEEEEECHHHHHHHHHHHHCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]