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The map label for this gene is ligB

Identifier: 66048020

GI number: 66048020

Start: 5685673

End: 5687358

Strand: Direct

Name: ligB

Synonym: Psyr_4796

Alternate gene names: 66048020

Gene position: 5685673-5687358 (Clockwise)

Preceding gene: 66048018

Following gene: 66048021

Centisome position: 93.3

GC content: 62.4

Gene sequence:

>1686_bases
ATGCTGCCTACCTTTCGCCTGATGACGTGCGCTATTGTGTTTGTTCTGCCCGGCCTCGGCCATGCCAGCCAATGCCCTGA
CTGGCCTGCAGCCAAGGCGCGCAGTGAGGTCACCGCCTTGCAACAGCAGATTGCCGAGTGGGATGACAGCTACCACCGGC
AGGGCATTTCGCAAATCGCGGACGAGCTCTACGATCAGGCGCGGCAAAAACTGAACCTGTGGCGCTCGTGCTTTGCCGTT
TCCACCCCCGAGTCCGATCCGCTAAAGACAGCAGTCGGCCCGTTGCCTCATCCCGTTCCGCACACGGGCGTCAACAAGCT
GGCCGACGAAGGCAGCGTCAAGGGCTGGCTCAAGGGCCGCAACGATCTGTGGATTCAACCCAAGGTCGATGGCGTAGCCG
TCACGCTGGTCTACGAAAAGGGCCGGCTTGTGCAGGCGATCAGTCGTGGTGACGGGCGCAAGGGGCAGGACTGGACCTCG
CAGGCGCGGCTGATCAAGGCCATTGCTCAACAACTGCCCCAGGCTGAATCCACAATCCTGCAAGGCGAGCTGTACTGGCG
GCTGGACGATCATATTCAGGCCGCATCCGGCAGCGTCAACGCGCGCAGCAAGGTCGCGGGACTGCTGGCACGGCAGACGA
TCAGCCCACAGCAGGCCGACGCCATCGGGCTGTTCGTCTGGGACTGGCCGAACGGGCCTGCCGATATGGCGCAACGACTC
GCGGGCCTGCAAGCCATGGGCTTTGAGGACAGCGCGGCCTATACGCATCCACTGGAAAACTACGTCCAGGCCGCCCGATG
GCGTGAGCATTGGTATCGCAATCCCCTGCCGTTCGCCACGGATGGCGTGATCCTGCGCCAGGGTCAGCGCCCACCTGCGC
AGCGCTGGCAAGCCAGTGCGCCTTACTGGATTGCCGCCTGGAAACATCCCTACGCCCAGGCGCTGGCCGAGGTGCGCAAG
GTCAACTTCAAGATCGGCCGCAGTGGCAGGATTACGCCGGTGCTGGAGCTGACCCCGGTGCGCCTCGATGATCGTACCGT
CAGCCGCATCAGCACGGGCTCTCTGCAGCGCTGGCAAACACTGGATATCAGGCCTGGCGATCAGATCGCGGTGAGTCTGG
CCGGGCTGACGATTCCAAGACTCGACGGCGTGGTGTCGCGTGCTGCCGAACGAGCCGACATGATCATTCCGCGAGCCGAC
GACTTTCATGAACTCAGCTGCTGGCAAGCCACTCCGGGCTGCGAAAGTCAGTTCCGCGCCAGACTGGCATGGCTCAGTGG
CAAGAAAGGCCTGGCGTTGCCCGGCGTTGGTCCGGGGACCTGGAACACACTTATCGACAACGGCCAAATCATGGGCTTAC
TCGATTGGATGACCCTGAATCATGCTGAGCTTGTTAACATTCCCGGCTTCGCTGAACGCAGTAGCGCTAAACTGCTCGAC
AGCCTGCAAACCGCACGCGAACGGCCGTTTCAGACATGGCTCAAGGCCATCGGACTGCCGCCCACGGGAGGCGCCAGACT
GCCCGACAACTGGCACGAGCTGGCTGGCCGCAGCGTCGAGCAATGGCAAGCAGAGCCCGGCATCGGTCCTGGTCGTGCAG
CCAGGCTCAGGGCTTTTTTTCAGGACCCACAGGTACAGGCACTGAGCCAGCAATTGCAGGCACAGAGCATTTCAGGTTTT
AAGTAG

Upstream 100 bases:

>100_bases
CGGCCTGCGCAATGGCAAAACCCGGTAACCAAGCCCCACGCCTTCACCACGCGACAACGACTAGCCTCATCGGGCCCATT
CCTAAAGCAAGGATGCTTCG

Downstream 100 bases:

>100_bases
CGGACGTTCATTTTTCTGTCCGTATTCAAACGGAGTTCATATGCAGTTTTTATCACCACTGATTCTTTTCACCAGTTGCA
CGCTGCTCGCCACAACCGTA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MLPTFRLMTCAIVFVLPGLGHASQCPDWPAAKARSEVTALQQQIAEWDDSYHRQGISQIADELYDQARQKLNLWRSCFAV
STPESDPLKTAVGPLPHPVPHTGVNKLADEGSVKGWLKGRNDLWIQPKVDGVAVTLVYEKGRLVQAISRGDGRKGQDWTS
QARLIKAIAQQLPQAESTILQGELYWRLDDHIQAASGSVNARSKVAGLLARQTISPQQADAIGLFVWDWPNGPADMAQRL
AGLQAMGFEDSAAYTHPLENYVQAARWREHWYRNPLPFATDGVILRQGQRPPAQRWQASAPYWIAAWKHPYAQALAEVRK
VNFKIGRSGRITPVLELTPVRLDDRTVSRISTGSLQRWQTLDIRPGDQIAVSLAGLTIPRLDGVVSRAAERADMIIPRAD
DFHELSCWQATPGCESQFRARLAWLSGKKGLALPGVGPGTWNTLIDNGQIMGLLDWMTLNHAELVNIPGFAERSSAKLLD
SLQTARERPFQTWLKAIGLPPTGGARLPDNWHELAGRSVEQWQAEPGIGPGRAARLRAFFQDPQVQALSQQLQAQSISGF
K

Sequences:

>Translated_561_residues
MLPTFRLMTCAIVFVLPGLGHASQCPDWPAAKARSEVTALQQQIAEWDDSYHRQGISQIADELYDQARQKLNLWRSCFAV
STPESDPLKTAVGPLPHPVPHTGVNKLADEGSVKGWLKGRNDLWIQPKVDGVAVTLVYEKGRLVQAISRGDGRKGQDWTS
QARLIKAIAQQLPQAESTILQGELYWRLDDHIQAASGSVNARSKVAGLLARQTISPQQADAIGLFVWDWPNGPADMAQRL
AGLQAMGFEDSAAYTHPLENYVQAARWREHWYRNPLPFATDGVILRQGQRPPAQRWQASAPYWIAAWKHPYAQALAEVRK
VNFKIGRSGRITPVLELTPVRLDDRTVSRISTGSLQRWQTLDIRPGDQIAVSLAGLTIPRLDGVVSRAAERADMIIPRAD
DFHELSCWQATPGCESQFRARLAWLSGKKGLALPGVGPGTWNTLIDNGQIMGLLDWMTLNHAELVNIPGFAERSSAKLLD
SLQTARERPFQTWLKAIGLPPTGGARLPDNWHELAGRSVEQWQAEPGIGPGRAARLRAFFQDPQVQALSQQLQAQSISGF
K
>Mature_561_residues
MLPTFRLMTCAIVFVLPGLGHASQCPDWPAAKARSEVTALQQQIAEWDDSYHRQGISQIADELYDQARQKLNLWRSCFAV
STPESDPLKTAVGPLPHPVPHTGVNKLADEGSVKGWLKGRNDLWIQPKVDGVAVTLVYEKGRLVQAISRGDGRKGQDWTS
QARLIKAIAQQLPQAESTILQGELYWRLDDHIQAASGSVNARSKVAGLLARQTISPQQADAIGLFVWDWPNGPADMAQRL
AGLQAMGFEDSAAYTHPLENYVQAARWREHWYRNPLPFATDGVILRQGQRPPAQRWQASAPYWIAAWKHPYAQALAEVRK
VNFKIGRSGRITPVLELTPVRLDDRTVSRISTGSLQRWQTLDIRPGDQIAVSLAGLTIPRLDGVVSRAAERADMIIPRAD
DFHELSCWQATPGCESQFRARLAWLSGKKGLALPGVGPGTWNTLIDNGQIMGLLDWMTLNHAELVNIPGFAERSSAKLLD
SLQTARERPFQTWLKAIGLPPTGGARLPDNWHELAGRSVEQWQAEPGIGPGRAARLRAFFQDPQVQALSQQLQAQSISGF
K

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily

Homologues:

Organism=Escherichia coli, GI87082305, Length=542, Percent_Identity=42.619926199262, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1788750, Length=578, Percent_Identity=24.9134948096886, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIGB_PSEU2 (Q4ZLZ9)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_237861.1
- ProteinModelPortal:   Q4ZLZ9
- SMR:   Q4ZLZ9
- STRING:   Q4ZLZ9
- GeneID:   3370346
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_4796
- NMPDR:   fig|205918.4.peg.3859
- eggNOG:   COG0272
- HOGENOM:   HBG336755
- OMA:   NARSKVA
- ProtClustDB:   PRK08097
- BioCyc:   PSYR205918:PSYR_4796-MONOMER
- HAMAP:   MF_01587
- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00532

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 62111; Mature: 62111

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPTFRLMTCAIVFVLPGLGHASQCPDWPAAKARSEVTALQQQIAEWDDSYHRQGISQIA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELYDQARQKLNLWRSCFAVSTPESDPLKTAVGPLPHPVPHTGVNKLADEGSVKGWLKGR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCHHCCCC
NDLWIQPKVDGVAVTLVYEKGRLVQAISRGDGRKGQDWTSQARLIKAIAQQLPQAESTIL
CCEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHEE
QGELYWRLDDHIQAASGSVNARSKVAGLLARQTISPQQADAIGLFVWDWPNGPADMAQRL
CCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHH
AGLQAMGFEDSAAYTHPLENYVQAARWREHWYRNPLPFATDGVILRQGQRPPAQRWQASA
HCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHCCCC
PYWIAAWKHPYAQALAEVRKVNFKIGRSGRITPVLELTPVRLDDRTVSRISTGSLQRWQT
CEEEEECCCHHHHHHHHHHHHHHEECCCCCCCEEEEECCCEECCHHHHHHCCCCCCCCEE
LDIRPGDQIAVSLAGLTIPRLDGVVSRAAERADMIIPRADDFHELSCWQATPGCESQFRA
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCHHHHHH
RLAWLSGKKGLALPGVGPGTWNTLIDNGQIMGLLDWMTLNHAELVNIPGFAERSSAKLLD
HHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEEHHHHCCCHHEECCCCCCCHHHHHHHH
SLQTARERPFQTWLKAIGLPPTGGARLPDNWHELAGRSVEQWQAEPGIGPGRAARLRAFF
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCHHHHHHHHH
QDPQVQALSQQLQAQSISGFK
CCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLPTFRLMTCAIVFVLPGLGHASQCPDWPAAKARSEVTALQQQIAEWDDSYHRQGISQIA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELYDQARQKLNLWRSCFAVSTPESDPLKTAVGPLPHPVPHTGVNKLADEGSVKGWLKGR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCHHCCCC
NDLWIQPKVDGVAVTLVYEKGRLVQAISRGDGRKGQDWTSQARLIKAIAQQLPQAESTIL
CCEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHEE
QGELYWRLDDHIQAASGSVNARSKVAGLLARQTISPQQADAIGLFVWDWPNGPADMAQRL
CCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHH
AGLQAMGFEDSAAYTHPLENYVQAARWREHWYRNPLPFATDGVILRQGQRPPAQRWQASA
HCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHCCCC
PYWIAAWKHPYAQALAEVRKVNFKIGRSGRITPVLELTPVRLDDRTVSRISTGSLQRWQT
CEEEEECCCHHHHHHHHHHHHHHEECCCCCCCEEEEECCCEECCHHHHHHCCCCCCCCEE
LDIRPGDQIAVSLAGLTIPRLDGVVSRAAERADMIIPRADDFHELSCWQATPGCESQFRA
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCHHHHHH
RLAWLSGKKGLALPGVGPGTWNTLIDNGQIMGLLDWMTLNHAELVNIPGFAERSSAKLLD
HHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEEHHHHCCCHHEECCCCCCCHHHHHHHH
SLQTARERPFQTWLKAIGLPPTGGARLPDNWHELAGRSVEQWQAEPGIGPGRAARLRAFF
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCHHHHHHHHH
QDPQVQALSQQLQAQSISGFK
CCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA