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The map label for this gene is metK [H]

Identifier: 66048018

GI number: 66048018

Start: 5682740

End: 5683930

Strand: Direct

Name: metK [H]

Synonym: Psyr_4794

Alternate gene names: 66048018

Gene position: 5682740-5683930 (Clockwise)

Preceding gene: 66048017

Following gene: 66048020

Centisome position: 93.26

GC content: 61.29

Gene sequence:

>1191_bases
ATGAGCGAATACTCACTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGACAAGATCGCCGACCAGATTTCCGACGC
GGTTCTGGACGCCATCATCGCTGAAGACAAATACGCCCGTGTAGCGTGCGAAACACTGGTCAAGACCGGTGTGGCGATCA
TCGCTGGCGAAGTGTCGACTTCGGCCTGGGTCGATCTGGAAGACATCGTGCGTAACGTCATCCTCGACATCGGCTACAAC
AGCTCCGACGTCGGTTTCGACGGCGCGACCTGCGGCGTGATGAACATCATCGGCAAGCAGTCGGTAGACATCGCCCAGGG
CGTTGACCGCAGCAAGCCAGAAGATCAGGGCGCTGGCGACCAGGGCCTGATGTTCGGCTATGCCAGCAACGAGACTGACG
TGCTGATGCCAGCGCCGATCACCTTCTCCCATCAGTTGGTGCAGCGTCAGGCCGAAGCGCGTAAATCCGGCCTGCTGCCG
TGGCTGCGCCCGGATGCCAAATCCCAGGTCACCTGCCGTTACGAAAACGGCAAGGTGGTCGGCGTCGACGCTATCGTATT
GTCGACCCAGCACAACCCGGATGTGTCGTACAAGGACCTGCGCGAAGGCGTGATGGAGCTGATCGTCAAGCACGTCATCC
CTGCGCACCTGCTGCACAAGGACACCCAGTTCCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTAGGCGAC
TGCGGTCTGACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCATGGCCCGTCACGGCGGCGGTGCTTTCTCCGGCAA
GGACCCATCGAAGGTTGACCGTTCGGCTGCCTACGCCGGTCGCTACGTCGCCAAGAACATCGTCGCTGCCGGCCTGGCCG
AGCGTTGCGAGATTCAGGTGTCCTACGCCATCGGCGTCGCTCAACCGACTTCGATCTCGCTGAACACGTTCGGCACCGGC
AAGCTGTCCGATGACAAGATCATCAAGCTGGTCCGCGACAACTTCGACCTGCGTCCTTACGCAATCACCACCATGCTCGA
CCTGCTGCACCCGATGTACCAGGCCACTGCAGCCTACGGCCACTTCGGTCGTATCCCGGTCGAAATGACCGTTGGCGATG
ACACCTTCACCGCCTTCACCTGGGAAAAAACCGACCGCGCCGACGCCCTGCGCGCAGCTGCCGGTCTGTAA

Upstream 100 bases:

>100_bases
TCTATGTAGGCTTACGTAATGGTTTCCAGATCCAGGTTCGCCATTTTCAGCGACCGGCTGGCGACACTCACCATCGGTAA
AATGAAGGAAACCCGTAGAG

Downstream 100 bases:

>100_bases
AAACCCAGAAAAAAGCCCCGAACGACCTGGTCGTTTCGGGGCTTTTTTTCGTCTGCGCGGCAGTCATTCCACGGTCACAC
TTCAAGCCCGCCGCGACACC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKYARVACETLVKTGVAIIAGEVSTSAWVDLEDIVRNVILDIGYN
SSDVGFDGATCGVMNIIGKQSVDIAQGVDRSKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLP
WLRPDAKSQVTCRYENGKVVGVDAIVLSTQHNPDVSYKDLREGVMELIVKHVIPAHLLHKDTQFHINPTGNFIIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTG
KLSDDKIIKLVRDNFDLRPYAITTMLDLLHPMYQATAAYGHFGRIPVEMTVGDDTFTAFTWEKTDRADALRAAAGL

Sequences:

>Translated_396_residues
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKYARVACETLVKTGVAIIAGEVSTSAWVDLEDIVRNVILDIGYN
SSDVGFDGATCGVMNIIGKQSVDIAQGVDRSKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLP
WLRPDAKSQVTCRYENGKVVGVDAIVLSTQHNPDVSYKDLREGVMELIVKHVIPAHLLHKDTQFHINPTGNFIIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTG
KLSDDKIIKLVRDNFDLRPYAITTMLDLLHPMYQATAAYGHFGRIPVEMTVGDDTFTAFTWEKTDRADALRAAAGL
>Mature_395_residues
SEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKYARVACETLVKTGVAIIAGEVSTSAWVDLEDIVRNVILDIGYNS
SDVGFDGATCGVMNIIGKQSVDIAQGVDRSKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPW
LRPDAKSQVTCRYENGKVVGVDAIVLSTQHNPDVSYKDLREGVMELIVKHVIPAHLLHKDTQFHINPTGNFIIGGPVGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTGK
LSDDKIIKLVRDNFDLRPYAITTMLDLLHPMYQATAAYGHFGRIPVEMTVGDDTFTAFTWEKTDRADALRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=383, Percent_Identity=54.8302872062663, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI5174529, Length=383, Percent_Identity=54.5691906005222, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=70.076726342711, Blast_Score=577, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=55.6135770234987, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=55.012853470437, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=54.4041450777202, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=388, Percent_Identity=53.0927835051546, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=49.6143958868895, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=52.9761904761905, Blast_Score=333, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=51.622418879056, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=52.411575562701, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=89, Percent_Identity=58.4269662921348, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=66, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=52.5641025641026, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=52.4804177545692, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=383, Percent_Identity=52.2193211488251, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=383, Percent_Identity=52.2193211488251, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=383, Percent_Identity=52.2193211488251, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=383, Percent_Identity=52.2193211488251, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=383, Percent_Identity=52.2193211488251, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=383, Percent_Identity=52.2193211488251, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=383, Percent_Identity=52.2193211488251, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=383, Percent_Identity=52.2193211488251, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=49.8701298701299, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=52.1367521367521, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42600; Mature: 42468

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKYARVACETLVKTGVAIIAGEVST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCC
SAWVDLEDIVRNVILDIGYNSSDVGFDGATCGVMNIIGKQSVDIAQGVDRSKPEDQGAGD
CCEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPWLRPDAKSQVTCRYENGKVV
CCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEE
GVDAIVLSTQHNPDVSYKDLREGVMELIVKHVIPAHLLHKDTQFHINPTGNFIIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEE
SYAIGVAQPTSISLNTFGTGKLSDDKIIKLVRDNFDLRPYAITTMLDLLHPMYQATAAYG
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
HFGRIPVEMTVGDDTFTAFTWEKTDRADALRAAAGL
CCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKYARVACETLVKTGVAIIAGEVST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCC
SAWVDLEDIVRNVILDIGYNSSDVGFDGATCGVMNIIGKQSVDIAQGVDRSKPEDQGAGD
CCEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPWLRPDAKSQVTCRYENGKVV
CCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEE
GVDAIVLSTQHNPDVSYKDLREGVMELIVKHVIPAHLLHKDTQFHINPTGNFIIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEE
SYAIGVAQPTSISLNTFGTGKLSDDKIIKLVRDNFDLRPYAITTMLDLLHPMYQATAAYG
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
HFGRIPVEMTVGDDTFTAFTWEKTDRADALRAAAGL
CCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA