Click here to switch to the map view.

The map label for this gene is rlmI [H]

Identifier: 66047988

GI number: 66047988

Start: 5638696

End: 5639892

Strand: Direct

Name: rlmI [H]

Synonym: Psyr_4764

Alternate gene names: 66047988

Gene position: 5638696-5639892 (Clockwise)

Preceding gene: 66047987

Following gene: 66047990

Centisome position: 92.53

GC content: 61.57

Gene sequence:

>1197_bases
ATGTCCCTGCCTAGCTTGCGCCTCAAAGCCAACGCCGACCGCCGCCTGCGCGCCGGTCATCTGTGGGTCTACAGCAACGA
GATCGATGTAGCCGCCACCCCACTGCATGGTTTTGCAGCCGGTGACCAGGCCATCCTCGAAGCTGCGGGCGGCAAGCCGC
TGGGCATCGTCGCCATGAGCCCCAACAACCTGATCTGCGCACGCCTGCTGTCGCGCGACATCAAGCTGCCGCTCGACAAG
TCTCTGCTCGTCCATCGCCTCAACGTTGCGCTGTCGCTGCGTGAGCGCCTGTTCGACAAGCCCTTCTACCGCCTGGTCTA
CGGTGACTCCGACCTGCTGCCGGGTCTGGTGGTCGATCGTTTCGGTGACATTCTGGTCGTTCAGCTGGCCTCGGCCACCA
TGGAAAACCACAAGGAAGACATCATTGCGGCGCTGGTTCAGGTCATCAAGCCAAGCGGCATCCTGTTCAAGAACGATTCG
GCGGCTCGCGATGCGGAAGGCCTGAATCGTTACGTCGAGACTGTTTTCGGTCTGGTACCGGAATGGGTCGCGCTGGAAGA
GAACGGCGTGAAGTTCGAAGCACCGGTCATGGCCGGGCAAAAAACCGGCTGGTTCTATGACCACCGCATGAACCGCGCGC
GCATCGCCCCTTACGTCAAAGGCAAGCGTGTGCTGGACCTGTACAGCTATATCGGCGGCTGGGGGATTCAGGCCGCGGCG
TTCGGTGCCAGCGACGTCACCTGCGTCGACGCGTCGTCCTTCGCCCTCGACGGCGTGGAGCGCAACGCAGCCCTGAACGG
CTTTGCAGAAAAAATGACCTGCATCGAAGGCGATGTCTTCGAAGCGCTCAAGGAACTGAAAGCCGCTGAAGAGCGTTTCG
ACGTGATCGTCGCTGATCCGCCTGCGTTCATCAAACGCAAGAAAGACATGAAAAACGGTGAAGGCGCCTACCGCCGCCTG
AACGAGCAAGCCATGCGCCTGCTCAGCAAGGACGGCATTCTGGTCAGCGCGTCGTGCTCGATGCACCTGCCGGAAGACGA
CCTGCAAAACATCCTCCTGACCAGCGCCCGCCATCTGGATCGCAACATCCAGCTGCTGGAACGCGGCGGCCAGGGCCCGG
ACCATCCGGTACACCCGGCGATCGCCGAAACGCGCTACATCAAGAGCATCACCTGCCGGTTGCTGCCCAACAGCTGA

Upstream 100 bases:

>100_bases
GCACTGTCTGCCTGACAGATTCCGTCAGTTAATTGCAGTCATCCCGGCCCAGCGCCGGGATGACGCGCTATAATCCCGCT
CTTTTTTCAGGAGCGACGTC

Downstream 100 bases:

>100_bases
TACCGAAGCGCCCCGACCTCTTCGCTCCTGCAAGAGCTCGCTGTTCCTGACTATGACTATCGTGCCAATGCTCTGCGTTG
GCACGATAGTCAAGACCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSLPSLRLKANADRRLRAGHLWVYSNEIDVAATPLHGFAAGDQAILEAAGGKPLGIVAMSPNNLICARLLSRDIKLPLDK
SLLVHRLNVALSLRERLFDKPFYRLVYGDSDLLPGLVVDRFGDILVVQLASATMENHKEDIIAALVQVIKPSGILFKNDS
AARDAEGLNRYVETVFGLVPEWVALEENGVKFEAPVMAGQKTGWFYDHRMNRARIAPYVKGKRVLDLYSYIGGWGIQAAA
FGASDVTCVDASSFALDGVERNAALNGFAEKMTCIEGDVFEALKELKAAEERFDVIVADPPAFIKRKKDMKNGEGAYRRL
NEQAMRLLSKDGILVSASCSMHLPEDDLQNILLTSARHLDRNIQLLERGGQGPDHPVHPAIAETRYIKSITCRLLPNS

Sequences:

>Translated_398_residues
MSLPSLRLKANADRRLRAGHLWVYSNEIDVAATPLHGFAAGDQAILEAAGGKPLGIVAMSPNNLICARLLSRDIKLPLDK
SLLVHRLNVALSLRERLFDKPFYRLVYGDSDLLPGLVVDRFGDILVVQLASATMENHKEDIIAALVQVIKPSGILFKNDS
AARDAEGLNRYVETVFGLVPEWVALEENGVKFEAPVMAGQKTGWFYDHRMNRARIAPYVKGKRVLDLYSYIGGWGIQAAA
FGASDVTCVDASSFALDGVERNAALNGFAEKMTCIEGDVFEALKELKAAEERFDVIVADPPAFIKRKKDMKNGEGAYRRL
NEQAMRLLSKDGILVSASCSMHLPEDDLQNILLTSARHLDRNIQLLERGGQGPDHPVHPAIAETRYIKSITCRLLPNS
>Mature_397_residues
SLPSLRLKANADRRLRAGHLWVYSNEIDVAATPLHGFAAGDQAILEAAGGKPLGIVAMSPNNLICARLLSRDIKLPLDKS
LLVHRLNVALSLRERLFDKPFYRLVYGDSDLLPGLVVDRFGDILVVQLASATMENHKEDIIAALVQVIKPSGILFKNDSA
ARDAEGLNRYVETVFGLVPEWVALEENGVKFEAPVMAGQKTGWFYDHRMNRARIAPYVKGKRVLDLYSYIGGWGIQAAAF
GASDVTCVDASSFALDGVERNAALNGFAEKMTCIEGDVFEALKELKAAEERFDVIVADPPAFIKRKKDMKNGEGAYRRLN
EQAMRLLSKDGILVSASCSMHLPEDDLQNILLTSARHLDRNIQLLERGGQGPDHPVHPAIAETRYIKSITCRLLPNS

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=35.1010101010101, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1787181, Length=246, Percent_Identity=32.9268292682927, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43858; Mature: 43727

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPSLRLKANADRRLRAGHLWVYSNEIDVAATPLHGFAAGDQAILEAAGGKPLGIVAMS
CCCCCCEEECCCCCCEEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCCCEEEEEEC
PNNLICARLLSRDIKLPLDKSLLVHRLNVALSLRERLFDKPFYRLVYGDSDLLPGLVVDR
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
FGDILVVQLASATMENHKEDIIAALVQVIKPSGILFKNDSAARDAEGLNRYVETVFGLVP
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
EWVALEENGVKFEAPVMAGQKTGWFYDHRMNRARIAPYVKGKRVLDLYSYIGGWGIQAAA
CCEEECCCCCEEECCEECCCCCCCCHHHCCCCHHCCCCCCCHHHHHHHHHHCCCCCHHEE
FGASDVTCVDASSFALDGVERNAALNGFAEKMTCIEGDVFEALKELKAAEERFDVIVADP
CCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECC
PAFIKRKKDMKNGEGAYRRLNEQAMRLLSKDGILVSASCSMHLPEDDLQNILLTSARHLD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHC
RNIQLLERGGQGPDHPVHPAIAETRYIKSITCRLLPNS
CHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEEECCCC
>Mature Secondary Structure 
SLPSLRLKANADRRLRAGHLWVYSNEIDVAATPLHGFAAGDQAILEAAGGKPLGIVAMS
CCCCCEEECCCCCCEEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCCCEEEEEEC
PNNLICARLLSRDIKLPLDKSLLVHRLNVALSLRERLFDKPFYRLVYGDSDLLPGLVVDR
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
FGDILVVQLASATMENHKEDIIAALVQVIKPSGILFKNDSAARDAEGLNRYVETVFGLVP
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
EWVALEENGVKFEAPVMAGQKTGWFYDHRMNRARIAPYVKGKRVLDLYSYIGGWGIQAAA
CCEEECCCCCEEECCEECCCCCCCCHHHCCCCHHCCCCCCCHHHHHHHHHHCCCCCHHEE
FGASDVTCVDASSFALDGVERNAALNGFAEKMTCIEGDVFEALKELKAAEERFDVIVADP
CCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECC
PAFIKRKKDMKNGEGAYRRLNEQAMRLLSKDGILVSASCSMHLPEDDLQNILLTSARHLD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHC
RNIQLLERGGQGPDHPVHPAIAETRYIKSITCRLLPNS
CHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA