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The map label for this gene is purU [H]

Identifier: 66047941

GI number: 66047941

Start: 5590242

End: 5591099

Strand: Direct

Name: purU [H]

Synonym: Psyr_4717

Alternate gene names: 66047941

Gene position: 5590242-5591099 (Clockwise)

Preceding gene: 66047940

Following gene: 66047942

Centisome position: 91.74

GC content: 57.93

Gene sequence:

>858_bases
ATGAGCCGCGCGCCCGACACATGGATTTTGACCGCCGACTGCCCCAGCATGCTGGGCACGGTAGACGCAGTGACGCGCTT
TCTGTTCGAGCAGGGTTGCTACGTCACAGAACACCACTCCTTCGATGACCGCCTCTCGGGACGCTTCTTCATCCGTGTGG
AGTTTCGTCAGCCCGAGGCTTTCGACGAACAGGCGTTCAGGGCCGGGCTGAGTGAGCGCGGCGAAACGTTTGGCATGATC
TTCGAACTCACCGCACCCAACTACCGGCCAAAAGTAGTGATCATGGTCTCGAAAGCCGATCATTGTCTCAATGACCTGCT
CTATCGCCAGCGTATCAATCAACTGTCCATGGACGTGGTCGCGGTGGTGTCCAATCACCCTGATCTTGAGCCACTGGCTG
GCTGGCACGGCATTACGTACTACCATTTCCCACTGGACCCGAACGACAAGCCTGCGCAGGAAGCCAAAGTCTGGCAGGTC
ATCGAAGAGTCTGGTGCAGAACTGGTTATTCTTGCCCGTTACATGCAGGTCCTGTCGCCGGACCTGTGTCGCAAGCTGGA
CGGCAAGGCGATCAATATCCACCACTCGTTGCTGCCCGGTTTCAAGGGCGCCAAGCCCTACCATCAGGCGTACAACAAGG
GGGTGAAGCTGGTCGGTGCCACGGCGCATTACATCAACAACGATCTGGACGAAGGTCCGATCATTGCCCAGGGCGTGGAA
GTGGTCGATCACAGTCACTATCCCGAAGACCTGATCGCCAAGGGTCGAGACATCGAAGGGCTGACACTGGCCCGCGCGGT
GGGTTATCACATCGAACGGCGGGTGTTCCTCAACGCCAACAGGACAGTGGTTTTGTAG

Upstream 100 bases:

>100_bases
CGCCCCGGTCGCTCCTACGGCCTTCGGCCAGAATCAAAAGCGGACGTGTTTACCACAACAAGTGCCTCGCTCAGGGGCAC
CGTACAGGAATCATTACCCT

Downstream 100 bases:

>100_bases
GCGCAAGCTGCAAGCTGCAAGCGATCCGTATTTCTCGCCGCTTGAAGCTTGCAACTGTTCTTCTCTGCTCTAAATCTGCT
TCATCACAATAAAAGCGAGG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSRAPDTWILTADCPSMLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPEAFDEQAFRAGLSERGETFGMI
FELTAPNYRPKVVIMVSKADHCLNDLLYRQRINQLSMDVVAVVSNHPDLEPLAGWHGITYYHFPLDPNDKPAQEAKVWQV
IEESGAELVILARYMQVLSPDLCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
VVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL

Sequences:

>Translated_285_residues
MSRAPDTWILTADCPSMLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPEAFDEQAFRAGLSERGETFGMI
FELTAPNYRPKVVIMVSKADHCLNDLLYRQRINQLSMDVVAVVSNHPDLEPLAGWHGITYYHFPLDPNDKPAQEAKVWQV
IEESGAELVILARYMQVLSPDLCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
VVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL
>Mature_284_residues
SRAPDTWILTADCPSMLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPEAFDEQAFRAGLSERGETFGMIF
ELTAPNYRPKVVIMVSKADHCLNDLLYRQRINQLSMDVVAVVSNHPDLEPLAGWHGITYYHFPLDPNDKPAQEAKVWQVI
EESGAELVILARYMQVLSPDLCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVEV
VDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=78, Percent_Identity=44.8717948717949, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI209869995, Length=78, Percent_Identity=44.8717948717949, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI209869993, Length=78, Percent_Identity=44.8717948717949, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=37.9061371841155, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=25.9067357512953, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=173, Percent_Identity=36.4161849710983, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24582400, Length=156, Percent_Identity=30.1282051282051, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32185; Mature: 32054

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAPDTWILTADCPSMLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPEA
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCCC
FDEQAFRAGLSERGETFGMIFELTAPNYRPKVVIMVSKADHCLNDLLYRQRINQLSMDVV
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHE
AVVSNHPDLEPLAGWHGITYYHFPLDPNDKPAQEAKVWQVIEESGAELVILARYMQVLSP
EEECCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCH
DLCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
HHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHCCEEEEEEHHHHHCCCCCCCCCHHHHHH
VVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL
HHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCEEEC
>Mature Secondary Structure 
SRAPDTWILTADCPSMLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPEA
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCCC
FDEQAFRAGLSERGETFGMIFELTAPNYRPKVVIMVSKADHCLNDLLYRQRINQLSMDVV
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHE
AVVSNHPDLEPLAGWHGITYYHFPLDPNDKPAQEAKVWQVIEESGAELVILARYMQVLSP
EEECCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCH
DLCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
HHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHCCEEEEEEHHHHHCCCCCCCCCHHHHHH
VVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL
HHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]