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The map label for this gene is dnaG [H]

Identifier: 66047867

GI number: 66047867

Start: 5500196

End: 5502151

Strand: Direct

Name: dnaG [H]

Synonym: Psyr_4640

Alternate gene names: 66047867

Gene position: 5500196-5502151 (Clockwise)

Preceding gene: 66047866

Following gene: 66047868

Centisome position: 90.26

GC content: 59.66

Gene sequence:

>1956_bases
ATGGCCGGGCTGATTCCCCAGAGCTTTATCGACGACCTTCTGAACCGCACCGATATTGTCGATGTTGTCAGTTCGCGCGT
GCAGCTCAAGAAAGCCGGGAAGAACTACACAGCGTGCTGCCCGTTCCACAAAGAGAAAACACCCTCTTTCAGCGTCAGCC
CGGACAAGCAGTTCTATTACTGCTTCGGTTGCGGCGCAGGCGGCAACGCGCTCGGCTTCATCATGGACCACGACAACCTG
GACTTCCCCCAGGCTGTCGAAGACCTGGCCAAGGCCGCCGGCATGGAAGTGCCTCGCGAACAAAGCGTAAAAGGCCAGAA
GCCTCGGCAACCGACCGATTCCCCGCTCTACCCGCTGCTGACCGCCGCCGCCGAGTTCTATCGCCAGGCCCTGAAAAGCC
ACCCGTCACGCAAGGCTGCGGTGGATTACCTCAAGGGCCGCGGGCTGTCCGGCGAAATCGCCAGGGACTTTGGCCTGGGC
TTCGCGCCGCCCGGCTGGGACAATCTGTACAAGCACCTGAGCAGCGACTCCTTGCAGCAAAAGGTGATGATCGATGCCGG
GCTGCTGATCGAGAACGCCGAAACCGGCAAACGCTATGACCGCTTCCGCGACCGGGTGATGTTTCCGATCCGTGATTCGC
GCGGGCGCGTCATTGCCTTTGGCGGCCGTGTACTGGGCGATGACAAACCCAAGTACCTGAACTCCCCGGAAACACCGGTT
TTCCACAAGGGCCAGGAGCTGTACGGGCTGTTCGAGGCGCGCAAATTCAATCGCAACCTCGATGAAATCATCGTCGTTGA
AGGCTACATGGACGTCATCGCCCTGGCCCAGCAAGGCTTGCGCAATGCCGTGGCGACGCTGGGCACTGCGACCAGCGAAG
AGCATTTGAAGCGCCTGTTCAGGGTCGTGCCCAACGTACTGTTCTGCTTCGACGGCGATCAGGCCGGACGTAACGCTGCA
TGGCGTGCGCTGGAAGCGACCCTGTCGAACCTGCAGGACGGACGCCGGGCGCGCTTTCTGTTTCTTCCCGAAGGCGAAGA
TCCGGACACACTGGTGCGCTCCGAAGGCACCGACGCGTTCAAGGCACGCATCAATCAGCACGCGCAGCCGCTGGCCGATT
ATTTTTTCGAGCAACTGACCAAAGAGTCCGACCCGCGCTCGCTGGAGGGCAAGGCGCACATGGCCACCCTCGCGGCGCCA
TTGATCGACAAAGTGCCCGGCGCGAACCTGCGTATTCTGATGCGTCAGCGCCTGACCGAAATTACCGGGCTGACCGGCGA
GGCCGTCAGCCATCTGGTGCAGAGCGCGCCGGCAGAGGCGCCACCGAGCTACGACCCGTATGTCGACTACGACGCCATGC
CTGATTTTGCCGATTACCAGCCACAAGGCGGTTACGAGCCTCAGCAGGAATGGACGCAAAAGAAAACCGGCGGTCAGAAT
CAAAAAAAATGGGACAACAAATCCTGGGGCAAAAAAGGCAAGCGCCAGGACTTCGAACCTCGAACGCCGCGTGTACCCAC
CGCCGTCGAGCCGCCCATGCAGGCCGCACTGAGAACCTTGCTGCATCACCCCGAATTGGCGGAAAAGGTCGAAAATGCCA
GCCATTTCGCTGCAGAAGACCAATCCAACGCACAACTGCTGGTCGCTCTTATCGAGGCCCGGCAAAAGAATCCGAACCTT
CGGTCGTTACAGTTGATAGCGCGATGGCACGGGACTGAACAGGGCCGCTTGCTGCGCGCACTGGCAGAAAAGGAATGGCT
GATTGAGGCCGATAACCTTGAACAACAGTTTTTCGACACTATAACTAGCTTATCCGCTCGCCAGCGCGAGCGCAGTCTGG
AACATCTGATCAGCAAGGCTCGGCAGACCGAGCTGAGCGCAGAAGAGAAAATTCAGCTTAGGGAACTGCTAAATCGCAAT
GTTCCCGCACAAACCCCGACCTCAACTGGCGCGTGA

Upstream 100 bases:

>100_bases
GTTTATTGCCGACGTCTTCAAAAAAGACGGCTGACAGGCTGACGGAACGCTCGTAGAGTCATCCGATACTTTTGCACCAC
GCTTGAATGATGAGAACGCC

Downstream 100 bases:

>100_bases
GGTCATAGCTCGGGTATAATCCTCGGCTTGTTTTTTGCCCGCCAAGACCTTCAGTGGATAGGGTGTTATGTCCGGAAAAG
CGCAACAGCAATCTCGTATC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDNL
DFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLLTAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLG
FAPPGWDNLYKHLSSDSLQQKVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV
FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAA
WRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAFKARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAP
LIDKVPGANLRILMRQRLTEITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN
QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAEDQSNAQLLVALIEARQKNPNL
RSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDTITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRN
VPAQTPTSTGA

Sequences:

>Translated_651_residues
MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDNL
DFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLLTAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLG
FAPPGWDNLYKHLSSDSLQQKVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV
FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAA
WRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAFKARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAP
LIDKVPGANLRILMRQRLTEITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN
QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAEDQSNAQLLVALIEARQKNPNL
RSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDTITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRN
VPAQTPTSTGA
>Mature_650_residues
AGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDNLD
FPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLLTAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLGF
APPGWDNLYKHLSSDSLQQKVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPVF
HKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAAW
RALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAFKARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAPL
IDKVPGANLRILMRQRLTEITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQNQ
KKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAEDQSNAQLLVALIEARQKNPNLR
SLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDTITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRNV
PAQTPTSTGA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=424, Percent_Identity=54.7169811320755, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72925; Mature: 72794

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNALGFIMDHDNLDFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLL
EEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCHHHHH
TAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLGFAPPGWDNLYKHLSSDSLQQ
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHH
KVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV
HHHHHCCCEEECCCCCHHHHHHHHHEECEEECCCCCEEEECCEEECCCCCCCCCCCCCCC
FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLF
CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RVVPNVLFCFDGDQAGRNAAWRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAF
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCHHH
KARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAPLIDKVPGANLRILMRQRLTE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAED
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
QSNAQLLVALIEARQKNPNLRSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCHHHHHHHH
ITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRNVPAQTPTSTGA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNALGFIMDHDNLDFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLL
EEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCHHHHH
TAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLGFAPPGWDNLYKHLSSDSLQQ
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHH
KVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV
HHHHHCCCEEECCCCCHHHHHHHHHEECEEECCCCCEEEECCEEECCCCCCCCCCCCCCC
FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLF
CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RVVPNVLFCFDGDQAGRNAAWRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAF
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCHHH
KARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAPLIDKVPGANLRILMRQRLTE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAED
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
QSNAQLLVALIEARQKNPNLRSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCHHHHHHHH
ITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRNVPAQTPTSTGA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]