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The map label for this gene is cca [H]

Identifier: 66047861

GI number: 66047861

Start: 5495417

End: 5496643

Strand: Direct

Name: cca [H]

Synonym: Psyr_4634

Alternate gene names: 66047861

Gene position: 5495417-5496643 (Clockwise)

Preceding gene: 66047860

Following gene: 66047864

Centisome position: 90.18

GC content: 60.23

Gene sequence:

>1227_bases
ATGCAGATTTATAAAGTGGGCGGTGCTGTACGCGACCGTCTTCTGGGTCAACCTGTCACGGACATCGACTGGGTCGTGGT
CGGCGCAAGCACCGAGGACATGCTGATCAAGGGCTATCGCCCGGTCGGTACAGATTTCCCGGTGTTTTTGCATCCGCTGA
CCAATGAGGAATACGCGCTGGCACGCACCGAGCGCAAGAGCGGCGTGGGCTATGGGGGCTTTGTTTTCCACGCCAGCCCC
GAGGTGACGCTTGAGCAGGATTTGATTCGGCGTGACCTGACCATCAATGCCATGGCTGAAGACAAGGACGGCAATTTAAC
CGATCCCTACAACGGCCAGAAGGATTTGCAAGCGCGGATATTGCGTCACGTTTCACCCGCATTCGCCGAAGATCCGCTCC
GGGTCTTGCGTGTGGCTCGATTCGCAGCACGCTACGCCGGTTACGGTTTTACCATCGCGCCCGAGACCCTGGGACTGATG
CGACAGCTCAGTGCATCCGGCGAACTCAAGGCGCTGACTGCCGAGCGAAGCTGGAAGGAAATCTCGCGCGCGCTCATGGA
AGAACAGCCTCAGGTGTTCATTCAGGTGCTGCACGACTGCGGCGCGCTCAAAGAGCTGATGCCCGAGGTCGAAGCTCTTT
TTGGTGTACCGCAGCCGGCGGCGCATCACCCGGAAATAGACACCGGCGTACACGTGCTCAGTGTTCTTGAGCAATCCGCA
CGCCACAAACACCCGCTGACGGTGCGCTGGGCCTGCTTGCTGCACGACCTCGGTAAAGGGCTGACGCCGGAAGCCGAATG
GCCAAGGCACATCGCTCATGAACACACCGGCCTGCGCCTGATAAAAGCGGTCAACGAGCGTTTTCGTGTGCCGAGGGACT
GCCAGGAACTGGCGCTGCTGGTCGGCCAGTACCATACCCACGGCCACCGTGCGCTGGAGTTGAAACCTTCGACCCTGCTG
GAGTTGCTGCAAAGCTTCGACGTCTACCGCCGCCCGCAGCGCTTTGAAGAGTTCATTGCGGCGTGCGAGATGGACGCCCG
AGGACGTCACGGCTTCGAACAACGCAGTTATCCACAGGCCGACTACCTGCGCGGCGCCGCCGAGGCCGCACGCGCGGTAT
CAGTACAGCCGTTGCTGGAACAGGGTCTGAAGGGCAAGGAACTGGGTGACGCGCTGAAGAACGAGCGCTTGAAGGCACTG
AAAACCTACAAGACCAGCACTGTGTGA

Upstream 100 bases:

>100_bases
TAAGCACTAGGGGAATAGGGTTTTAAACGCTTGTTCATGCAGCTTTAGCCTCCGCAAGTACGACACAAGGTTTATCCTGT
CGCGCTTACGGAGGCTTTTT

Downstream 100 bases:

>100_bases
CGCGGAGCGTGAGGAACGAGAGGTGTCCGGGAGAGCACTTTTCGTACCTATGCGCAGCCATGGACGCTCTGCACTCGGGC
TGAAAGACGAGGATGACCTC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQIYKVGGAVRDRLLGQPVTDIDWVVVGASTEDMLIKGYRPVGTDFPVFLHPLTNEEYALARTERKSGVGYGGFVFHASP
EVTLEQDLIRRDLTINAMAEDKDGNLTDPYNGQKDLQARILRHVSPAFAEDPLRVLRVARFAARYAGYGFTIAPETLGLM
RQLSASGELKALTAERSWKEISRALMEEQPQVFIQVLHDCGALKELMPEVEALFGVPQPAAHHPEIDTGVHVLSVLEQSA
RHKHPLTVRWACLLHDLGKGLTPEAEWPRHIAHEHTGLRLIKAVNERFRVPRDCQELALLVGQYHTHGHRALELKPSTLL
ELLQSFDVYRRPQRFEEFIAACEMDARGRHGFEQRSYPQADYLRGAAEAARAVSVQPLLEQGLKGKELGDALKNERLKAL
KTYKTSTV

Sequences:

>Translated_408_residues
MQIYKVGGAVRDRLLGQPVTDIDWVVVGASTEDMLIKGYRPVGTDFPVFLHPLTNEEYALARTERKSGVGYGGFVFHASP
EVTLEQDLIRRDLTINAMAEDKDGNLTDPYNGQKDLQARILRHVSPAFAEDPLRVLRVARFAARYAGYGFTIAPETLGLM
RQLSASGELKALTAERSWKEISRALMEEQPQVFIQVLHDCGALKELMPEVEALFGVPQPAAHHPEIDTGVHVLSVLEQSA
RHKHPLTVRWACLLHDLGKGLTPEAEWPRHIAHEHTGLRLIKAVNERFRVPRDCQELALLVGQYHTHGHRALELKPSTLL
ELLQSFDVYRRPQRFEEFIAACEMDARGRHGFEQRSYPQADYLRGAAEAARAVSVQPLLEQGLKGKELGDALKNERLKAL
KTYKTSTV
>Mature_408_residues
MQIYKVGGAVRDRLLGQPVTDIDWVVVGASTEDMLIKGYRPVGTDFPVFLHPLTNEEYALARTERKSGVGYGGFVFHASP
EVTLEQDLIRRDLTINAMAEDKDGNLTDPYNGQKDLQARILRHVSPAFAEDPLRVLRVARFAARYAGYGFTIAPETLGLM
RQLSASGELKALTAERSWKEISRALMEEQPQVFIQVLHDCGALKELMPEVEALFGVPQPAAHHPEIDTGVHVLSVLEQSA
RHKHPLTVRWACLLHDLGKGLTPEAEWPRHIAHEHTGLRLIKAVNERFRVPRDCQELALLVGQYHTHGHRALELKPSTLL
ELLQSFDVYRRPQRFEEFIAACEMDARGRHGFEQRSYPQADYLRGAAEAARAVSVQPLLEQGLKGKELGDALKNERLKAL
KTYKTSTV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=226, Percent_Identity=31.4159292035398, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=56.9306930693069, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI87081691, Length=172, Percent_Identity=32.5581395348837, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45811; Mature: 45811

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYKVGGAVRDRLLGQPVTDIDWVVVGASTEDMLIKGYRPVGTDFPVFLHPLTNEEYAL
CEEEECCHHHHHHHCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGVGYGGFVFHASPEVTLEQDLIRRDLTINAMAEDKDGNLTDPYNGQKDLQARI
HHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYAGYGFTIAPETLGLMRQLSASGELKALTAERSWKE
HHHCCHHHHHCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCEEEHHHHHHHHH
ISRALMEEQPQVFIQVLHDCGALKELMPEVEALFGVPQPAAHHPEIDTGVHVLSVLEQSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
RHKHPLTVRWACLLHDLGKGLTPEAEWPRHIAHEHTGLRLIKAVNERFRVPRDCQELALL
HCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VGQYHTHGHRALELKPSTLLELLQSFDVYRRPQRFEEFIAACEMDARGRHGFEQRSYPQA
HHHHHCCCCEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHCCCCHH
DYLRGAAEAARAVSVQPLLEQGLKGKELGDALKNERLKALKTYKTSTV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQIYKVGGAVRDRLLGQPVTDIDWVVVGASTEDMLIKGYRPVGTDFPVFLHPLTNEEYAL
CEEEECCHHHHHHHCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGVGYGGFVFHASPEVTLEQDLIRRDLTINAMAEDKDGNLTDPYNGQKDLQARI
HHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYAGYGFTIAPETLGLMRQLSASGELKALTAERSWKE
HHHCCHHHHHCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCEEEHHHHHHHHH
ISRALMEEQPQVFIQVLHDCGALKELMPEVEALFGVPQPAAHHPEIDTGVHVLSVLEQSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
RHKHPLTVRWACLLHDLGKGLTPEAEWPRHIAHEHTGLRLIKAVNERFRVPRDCQELALL
HCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VGQYHTHGHRALELKPSTLLELLQSFDVYRRPQRFEEFIAACEMDARGRHGFEQRSYPQA
HHHHHCCCCEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHCCCCHH
DYLRGAAEAARAVSVQPLLEQGLKGKELGDALKNERLKALKTYKTSTV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA