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The map label for this gene is ureF2 [H]

Identifier: 66047679

GI number: 66047679

Start: 5317743

End: 5318417

Strand: Direct

Name: ureF2 [H]

Synonym: Psyr_4452

Alternate gene names: 66047679

Gene position: 5317743-5318417 (Clockwise)

Preceding gene: 66047678

Following gene: 66047680

Centisome position: 87.27

GC content: 64.59

Gene sequence:

>675_bases
GTGAACAGCGCCTGGGCGCTGTTGCGCCTGGCCAGTCCGCAGTTGCCTATCGGTGGCTACAGCTATTCGCAAGGGCTGGA
AATGGCAGTGGAGCAATCCATTGTGGTGGACCCGCAGACTGCGGGCCGCTGGATCGGCGATCAACTGCTGCTCAACCTCG
CGCGTTTCGAGGCCCCGCTGCTGCTGGCTCATTGCGATGCAGCGGCAGCCGGTGACTGGGGCCAGTTGCTGCAAGTCAGC
GAGCAGCATCGCGCCAGCCGCGAGACTCGCGAGCTGCATCTGGAAAGCCGGCAGATGGGCTACTCGCTGAAGCAGTTGCT
CAATGGCCTGCCTGAACTGGATCGTGATGCACGGCATTTTCTGCAGCAGACTGCCGAGCCGCACCTGGCCCTTGGCTGGG
CGCTGGCCGCACGCGCCTGGCAGATCAGCCCGCAGGATGCGCTGGCCGCCTGGCTATGGAGCTGGCTTGAAAACCAGCTG
GCCGTGCTGATGAAAACCCTGCCGCTGGGCCAGCAAGCCGCCCAGCGCCTGACCAGCGAGCTGTTGCCGTTATTGCAGCA
GGCGCAGGTCAGTGCCAGCGCACAAGATCCCTGCCATGCCGGCAGCGCAGCCTTCGGTCTGTCCCTGGCGAGCATGGCGC
ACGAACGTCAATACAGCCGGTTATTCCGGTCCTGA

Upstream 100 bases:

>100_bases
AGCCGGAACATGGCGCCTACGGCGGTGGGCATCATCACTCGCGTGCAGGCGAGGAGGATTTCAACTATCCGCCGCGCATG
CACCAGTTCGGCGTGCGCAA

Downstream 100 bases:

>100_bases
TCAGTCCTTATGGAGAACTCAATGAACAGCCAACCTCTGCGTGTCGGAATCGGTGGCCCGGTCGGATCCGGCAAGACGGC
GCTGACCCTGGCGCTGTGCC

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNSAWALLRLASPQLPIGGYSYSQGLEMAVEQSIVVDPQTAGRWIGDQLLLNLARFEAPLLLAHCDAAAAGDWGQLLQVS
EQHRASRETRELHLESRQMGYSLKQLLNGLPELDRDARHFLQQTAEPHLALGWALAARAWQISPQDALAAWLWSWLENQL
AVLMKTLPLGQQAAQRLTSELLPLLQQAQVSASAQDPCHAGSAAFGLSLASMAHERQYSRLFRS

Sequences:

>Translated_224_residues
MNSAWALLRLASPQLPIGGYSYSQGLEMAVEQSIVVDPQTAGRWIGDQLLLNLARFEAPLLLAHCDAAAAGDWGQLLQVS
EQHRASRETRELHLESRQMGYSLKQLLNGLPELDRDARHFLQQTAEPHLALGWALAARAWQISPQDALAAWLWSWLENQL
AVLMKTLPLGQQAAQRLTSELLPLLQQAQVSASAQDPCHAGSAAFGLSLASMAHERQYSRLFRS
>Mature_224_residues
MNSAWALLRLASPQLPIGGYSYSQGLEMAVEQSIVVDPQTAGRWIGDQLLLNLARFEAPLLLAHCDAAAAGDWGQLLQVS
EQHRASRETRELHLESRQMGYSLKQLLNGLPELDRDARHFLQQTAEPHLALGWALAARAWQISPQDALAAWLWSWLENQL
AVLMKTLPLGQQAAQRLTSELLPLLQQAQVSASAQDPCHAGSAAFGLSLASMAHERQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter [H]

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002639 [H]

Pfam domain/function: PF01730 UreF [H]

EC number: NA

Molecular weight: Translated: 24751; Mature: 24751

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAWALLRLASPQLPIGGYSYSQGLEMAVEQSIVVDPQTAGRWIGDQLLLNLARFEAPL
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCHH
LLAHCDAAAAGDWGQLLQVSEQHRASRETRELHLESRQMGYSLKQLLNGLPELDRDARHF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LQQTAEPHLALGWALAARAWQISPQDALAAWLWSWLENQLAVLMKTLPLGQQAAQRLTSE
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPLLQQAQVSASAQDPCHAGSAAFGLSLASMAHERQYSRLFRS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSAWALLRLASPQLPIGGYSYSQGLEMAVEQSIVVDPQTAGRWIGDQLLLNLARFEAPL
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCHH
LLAHCDAAAAGDWGQLLQVSEQHRASRETRELHLESRQMGYSLKQLLNGLPELDRDARHF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LQQTAEPHLALGWALAARAWQISPQDALAAWLWSWLENQLAVLMKTLPLGQQAAQRLTSE
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPLLQQAQVSASAQDPCHAGSAAFGLSLASMAHERQYSRLFRS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]